禽营养与饲料 POULTRY NUTRITION AND FEED

微生态制剂对产蛋后期种母鸡生产性能、肠道菌群和消化吸收的影响

  • 陈猜猜 ,
  • 张红星 ,
  • 金君华 ,
  • 谢远红 ,
  • 熊利霞 ,
  • 刘慧
展开
  • 北京农学院食品科学与工程学院, 微生态制剂关键技术开发北京市工程实验室, 食品质量与安全北京实验室, 农产品有害微生物及农残安全检测与控制北京市重点实验室, 北京 102206
陈猜猜(1996-),女,河南商丘人,硕士研究生,研究方向为食品微生物学技术。E-mail:935570764@qq.com

收稿日期: 2021-05-06

  网络出版日期: 2021-12-16

基金资助

宁夏回族自治区重点研发计划项目(2020BBF02023)

Effects of Microecologics on Performance, Intestinal Flora and Digestive Absorption of Breeding Hens in Late Laying Period

  • CHEN Caicai ,
  • ZHANG Hongxing ,
  • JIN Junhua ,
  • XIE Yuanhong ,
  • XIONG Lixia ,
  • LIU Hui
Expand
  • Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Beijing Laboratory of Food Quality and Safety, Beijing Engineering Laboratory of Probiotics Key Technology Development, Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China

Received date: 2021-05-06

  Online published: 2021-12-16

摘要

本试验旨在研究副干酪乳杆菌KL1和枯草芽孢杆菌Liu-c1微生态制剂对产蛋后期种母鸡的生产性能、肠道菌群和消化吸收能力的影响。将384只54周龄的京红1号父母代种母鸡随机分为4组(每组6个重复,每个重复16只),空白组饲喂基础饲粮,KL1组、Liu-c1组和复合组在基础饲粮中分别添加8.0×107 CFU/只的副干酪乳杆菌KL1、枯草芽孢杆菌Liu-c1和复合微生态制剂(副干酪乳杆菌KL1:枯草芽孢杆菌Liu-c1=1:1)。结果表明:1)复合组产蛋率较空白组提高8.84%(P<0.01),较KL1组和Liu-c1组分别提高2.90%和3.29%(P<0.05)。复合组种蛋合格率较空白组提高14.43%(P<0.01),较Liu-c1组提高4.84%(P<0.05),与KL1组无显著性差异(P>0.05)。复合组料蛋比较空白组降低5.45%(P<0.01),较KL1组降低3.26%(P<0.05),与Liu-c1组无显著性差异(P>0.05)。2)与空白组回肠菌群多样性相比,复合组厚壁菌门相对丰度增加35.68%(P<0.05),变形菌门相对丰度减少92.02%(P<0.01),乳杆菌属和芽孢菌属相对丰度分别增加111.73%和300.00%(P<0.01)。与空白组相比,复合组乳酸杆菌和芽孢杆菌数量分别增加34.51%和23.37%(P<0.05)。3)与空白组相比,复合组十二指肠鸡胰蛋白酶活性增加15.98%(P<0.01),中性蛋白酶活性和粗蛋白质表观消化率分别增加13.13%和48.50%(P>0.05),十二指肠和回肠绒腺比分别增加150.44%和51.87%(P<0.01)。综上所述,复合微生态制剂(副干酪乳杆菌KL1:枯草芽孢杆菌Liu-c1=1:1)对提高产蛋后期种母鸡的肠道蛋白酶活性、绒腺比、粗蛋白质表观消化率及调节肠道菌群等效果最佳。

本文引用格式

陈猜猜 , 张红星 , 金君华 , 谢远红 , 熊利霞 , 刘慧 . 微生态制剂对产蛋后期种母鸡生产性能、肠道菌群和消化吸收的影响[J]. 动物营养学报, 2021 , 33(12) : 6792 -6801 . DOI: 10.3969/j.issn.1006-267x.2021.12.021

Abstract

This experiment was conducted to investigate the effects of Lactobacillus paracasei KL1 and Bacillus subtilis Liu-c1 microecologics on the performance, intestinal flora and digestive absorption of breeding hens in late laying hens. A total of 384 Jinghong No.1 laying hens at 54 weeks of age were randomly assigned to 4 groups, with 6 replicates of 16 birds. Blank group were fed a basal diet, and those in KL1, Liu-c1 and compound groups were fed the basal diets supplemented with 8.0×107 CFU/hen of KL1, Liu-c1 and compound microecologics (Lactobacillus paracasei KL1:Bacillus subtilis Liu-c1=1:1), respectively. The results showed as follows:1) laying rate in compound group was significantly increased by 8.84% (P<0.01), and that in KL1 group and Liu-c1 group was increased by 2.90% and 3.29% (P<0.05) compared with blank group. Hatchable egg rate in the compound group was significantly increased by 14.43% compared with blank group (P<0.01), and by 4.84% compared with Liu-c1 group (P<0.05), but there was no significant difference with the KL1 group (P>0.05). Feed/egg in the compound group was significantly increased by 5.45% compared with blank group (P<0.01), and by 3.26% compared with KL1 group (P<0.05), but there was no significant difference with the Liu-c1 group (P>0.05). 2) Compared with blank group, the relative abundance of Firmicutes in compound group was significantly increased by 35.68% (P<0.05), that of Proteobacteria was significantly decreased by 92.02% (P<0.01), and that of Lactobacillus and Bacillus were significantly increased by 111.73% and 300.00% (P<0.01), respectively, the number of Lactobacillus and Bacillus was significantly increased by 34.51% and 23.37% (P<0.05), respectively. 3) Compared with blank group, the trypsin activity in duodenum in compound group was significantly increased by 15.98% (P<0.01), the activity of neutral protease and the apparent digestibility of crude protein were increased by 13.13% and 48.50% (P>0.05), respectively, and the villous height/crypt depth in duodenum and ileum were significantly increased by 150.44% and 51.87% (P<0.01), respectively. In conclusion, compound probiotics (Lactobacillus paracei KL1:Bacillus subtilis Liuc-c1=1:1) has the best effect on improving intestinal protease activity, villous height/crypt depth, crude protein apparent digestibility and intestinal flora of breeding hens in late laying period.

参考文献

[1] DOREEN L,MÉSZÁROS G,ELLEN E D.Survival analysis of white leghorn laying hens in the early and late production period[J].Agriculture Conspectus Scientificus,2017,82(2):179-183.
[2] LIU Y,LI Y,LIU H N,et al.Effect of quercetin on performance and egg quality during the late laying period of hens[J].British Poultry Science,2013,54(4):510-514.  
[3] 中华人民共和国农业部.中华人民共和国农业部公告第2045号[EB/OL].(2013-12-30)[2021-4-12].http://www.moa.gov.cn/nybgb/2014/dyq/201712/t20171219_6104350.htm. The Ministry of Agriculture of the People's Republic of China.Announcement of the ministry of agriculture of the people's republic of China No. 2045[EB/OL].(2013-12-30)[2021-4-12].http://www.moa.gov.cn/nybgb/2014/dyq/201712/t20171219_6104350.htm.(in Chinese)
[4] LANGE L D.Nutribiotics could replace antibiotics in feed[J].Poultry World,2005,21(10):26-28.
[5] GAGGA F,MATTARELLI P,BIAVATI B.Probiotics and prebiotics in animal feeding for safe food production[J].International Journal of Food Microbiology,2010,141(S1):S15-S28.
[6] PATTERSON J A,BURKHOLDER K M.Application of prebiotics and probiotics in poultry production[J].Poultry Science,2003,82(4):627-631.  
[7] WAN X Q,CUI Z X,LI H M,et al.Prevention of Escherichia coli-induced diarrhea with microecological complex preparation in swine[J].Biomedical Research,2014,25(1):79-83.
[8] ZHANG S,ZHONG G,SHAO D,et al.Dietary supplementation with Bacillus subtilis promotes growth performance of broilers by altering the dominant microbial community[J].Poultry Science,2021,100(3):100935.
[9] JEONG J S,KIM I H.Effect of Bacillus subtilis C-3102 spores as a probiotic feed supplement on growth performance,noxious gas emission,and intestinal microflora in broilers[J].Poultry Science,2014,93(12):3097-3103.  
[10] BEHNAMIFAR A,RAHIMI S,KARIMI TORSHIZI M A.Effect of probiotic,thyme,garlic and caraway herbal extracts on the quality and quantity of eggs,blood parameters,intestinal bacterial population and histomorphology in laying hens[J].Journal of Medicinal Plants and By-products,2015,4(1):121-128.
[11] 黄晨轩,岳巧娴,徐静,等.复合微生态制剂对产蛋后期蛋鸡养分消化率、免疫功能和肠道菌群的影响[J].中国家禽,2018,40(6):24-28. HUANG C X,YUE Q X,XU J,et al.Effects of compound probiotics on nutrient utilization,immune function and intestinal microflora of laying hens at late laying period[J].China Poultry,2018,40(6):24-28.(in Chinese)
[12] ZHANG J L,XIE Q M,JI J,et al.Different combinations of probiotics improve the production performance,egg quality,and immune response of layer hens[J].Poultry Science,2012,91(11):2755-2760.  
[13] LEI K,LI Y L,YU D Y,et al.Influence of dietary inclusion of Bacillus licheniformis on laying performance,egg quality,antioxidant enzyme activities,and intestinal barrier function of laying hens[J].Poultry Science,2013,92(9):2389-2395.  
[14] 刘慧,熊利霞,易欣欣,等.藏灵菇中高产胞外多糖乳酸菌的筛选及其发酵性能的研究[J].食品科学,2007,28(5):211-215. LIU H,XIONG L X,YI X X,et al.Study on screening and fermentation capability of lactobacter yielding exopolysaccharide from kefir grains[J].Food Science,2007,28(5):211-215.(in Chinese)
[15] HUI L,XIE Y H,HAN T,et al.Purification and structure study on exopolysaccharides produced by Lactobacillus paracasei KL1-Liu from Tibetan Kefir[J].Advanced Materials Research,2013,781/784:1513-1518.
[16] 刘慧,张红星,熊利霞,等.一种产胆盐水解酶副干酪乳杆菌干粉活菌制剂的生产技术:CN104357359A[P].2015-02-18. LIU H,ZHANG H X,XIONG L X,et al.Production technology of a live dry powder preparation of Lactobacillus paracasei producing bile brine hydrolysase:CN104357359A[P].2015-02-18.(in Chinese)
[17] STACKEBRANDT E,GOEBEL B M.Taxonomic note:a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology[J].International Journal of Systematic Bacteriology,1994,44(4):846-849.
[18] 彭豫东,康克浪,曲湘勇,等.枯草芽孢杆菌对石门土鸡生长性能、屠宰性能、血清抗氧化指标和肠道形态的影响[J].动物营养学报,2019,31(5):2119-2126. PENG Y D,KANG K L,QU X Y,et al.Effects of Bacillus subtilis on growth performance,slaughter performance,serum antioxidant indexes and intestinal morphology of Shimen chickens[J].Chinese Journal of Animal Nutrition,2019,31(5):2119-2126. (in Chinese)
[19] CHEN C C,LI J Y,ZHANG H X,et al.Effects of a probiotic on the growth performance,intestinal flora,and immune function of chicks infected with Salmonella pullorum[J].Poultry Science,2020,99(11):5316-5323.  
[20] 刘慧,张红星,谢远红,等.一种利用产胆盐水解酶的副干酪乳杆菌降低鸡蛋胆固醇的方法:CN106635886A[P].2017-05-10. LIU H,ZHANG H X,XIE Y H,et al.A method for reducing cholesterol in eggs using Lactobacillus paracasei producing bile brine hydrolysase:CN106635886A[P].2017-05-10.(in Chinese)
[21] 于雷,程高宾,邹本革,等.芽孢杆菌微生态制剂对京红1号蛋鸡产蛋性能和蛋品质的影响[J].中国家禽,2020,42(4):58-62. YU L,CHENG G B,ZOU B G,et al.Effects of microecological preparations of Bacillus on laying performance and egg quality of Jinghong laying hens[J].China Poultry,2020,42(4):58-62.(in Chinese)
[22] ABDELQADER A,IRSHAID R,AL-FATAFTAH A R.Effects of dietary probiotic inclusion on performance,eggshell quality,cecal microflora composition,and tibia traits of laying hens in the late phase of production[J].Tropical Animal Health and Production,2013,45(4):1017-1024.  
[23] 胡昌艳.复合微生物制剂对蛋鸡生产性能、养分代谢及发酵粪中氨气和硫化氢散发量的影响[D].硕士学位论文.乌鲁木齐:新疆农业大学,2016. HU C Y.The effect of compound microecological preparation on production performance,nutrient utilization and fecal emission of ammonia and sulfureted hydrogen of layers[D].Master's Thesis.Urumqi:Xinjiang Agricultural University,2016.(in Chinese)
[24] 闫昭明,李文睿,刘霜莉,等.单端孢霉烯族毒素的致病机理及防治措施研究进展[J].动物营养学报,2020,32(6):2543-2550. YAN Z M,LI W R,LIU S L,et al.Research advances on pathogenic mechanism and control measures of trichothecenes mycotoxin[J].Chinese Journal of Animal Nutrition,2020,32(6):2543-2550.(in Chinese)
[25] ROSSI C N,TAKABAYASHI C R,ONO M A,et al.Exposure of laying hens to mycotoxins through naturally contaminated feed[J].World Mycotoxin Journal,2013,6(2):199-207.  
[26] AKANDE T O,SALAMI T K,SALAKO A O.Nutrient stability in mould-infested feed and mitigating effect of dietary supplemental vitamins in brown laying hens[J].Tropical Animal Health and Production,2020,52(1):293-299.  
[27] 刘慧,王世平,冉冉,等.藏灵菇源酸奶复合菌发酵剂对肠道菌群平衡的作用[J].中国食品学报,2011,11(6):7-12. LIU H,WANG S P,RAN R,et al.The effection on enterobacteria balance from complex bacteria leaven of Tibetan Kefir[J].Journal of Chinese Institute of Food Science and Technology,2011,11(6):7-12.(in Chinese)
[28] GANDRA J R,OLIVEIRA E R,DE SENA GANDRA E R,et al.Inoculation of Lactobacillus buchneri alone or with Bacillus subtilis and total losses,aerobic stability,and microbiological quality of sunflower silage[J].Journal of Applied Animal Research,2017,45(1):609-614.  
[29] ABDELQADER A,AL-FATAFTAH A R,DAŞ G.Effects of dietary Bacillus subtilis and inulin supplementation on performance,eggshell quality,intestinal morphology and microflora composition of laying hens in the late phase of production[J].Animal Feed Science and Technology,2013,179(1/4):103-111.
[30] YANG J J,ZHAN K,ZHANG M H.Effects of the use of a combination of two Bacillus species on performance,egg quality,small intestinal mucosal morphology,and cecal microbiota profile in aging laying hens[J].Probiotics and Antimicrobial Proteins,2020,12(1):204-213.  
[31] WANG H S,NI X E,QING X D,et al.Live probiotic Lactobacillus johnsonii BS15 promotes growth performance and lowers fat deposition by improving lipid metabolism, intestinal development,and gut microflora in broilers[J].Frontiers in Microbiology,2017,8:1073.
[32] RAMLUCKEN U,RAMCHURAN S O,MOONSAMY G,et al.A novel Bacillus based multi-strain probiotic improves growth performance and intestinal properties of Clostridium perfringens challenged broilers[J].Poultry Science,2020,99(1):331-341.  
[33] LARA E C,BASSO F C,DE ASSIS F D,et al.Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum[J].Animal Production Science,2016,56(11):1867-1874.  
[34] XING G R,LANG X R,WU J A,et al.Mechanism of the effect of Bacillus coagulans on production performance of laying hens in late laying period[J].Animal Husbandry and Feed Science,2019,11(Z2):125-130.
文章导航

/