禽营养与饲料 POULTRY NUTRITION AND FEED

饲粮添加化香果单宁对肉鸡免疫器官指数、血浆抗氧化和生化指标及盲肠微生物区系的影响

  • 从光雷 ,
  • 肖蕴祺 ,
  • 张倩雲 ,
  • 张雪 ,
  • 张倚剑 ,
  • 邵丹 ,
  • 张安 ,
  • 施寿荣
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  • 1. 中国农业科学院家禽研究所, 扬州 225125;
    2. 扬州大学动物科学与技术学院, 扬州 225009
从光雷(1996-),男,安徽六安人,硕士研究生,从事动物营养与肠道健康研究。E-mail:MZ120181027@yzu.edu.cn

收稿日期: 2021-04-28

  网络出版日期: 2021-12-16

基金资助

江苏现代农业(肉鸡)产业技术体系建设项目(JATS[2019]379)

Effects of Dietary Plotytarya strohilacea Sieb. et Zuce Tannin on Immune Organ Indexes, Plasma Antioxidant and Biochemical Indexes and Cecal Microflora of Broilers

  • CONG Guanglei ,
  • XIAO Yunqi ,
  • ZHANG Qianyun ,
  • ZHANG Xue ,
  • ZHANG Yijian ,
  • SHAO Dan ,
  • ZHANG An ,
  • SHI Shourong
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  • 1. Institute of Poultry, Chinese Academy of Agricultural Sciences, Yangzhou 225125, China;
    2. School of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2021-04-28

  Online published: 2021-12-16

摘要

本试验旨在研究饲粮添加化香果单宁对肉鸡免疫器官指数、血浆抗氧化和生化指标及盲肠微生物区系的影响。选取288羽1日龄雄性爱拔益加(AA)肉鸡,随机分为4组,每组6个重复,每个重复12羽。对照组(PT0组)饲喂基础饲粮,试验组分别在基础饲粮中添加100(PT100组)、500(PT500组)和1 000 mg/kg(PT1 000组)化香果单宁。试验期42 d。结果表明:1)PT500和PT1 000组21日龄肉鸡的法氏囊指数显著高于PT0和PT100组(P<0.05)。饲粮添加化香果单宁具有提高21日龄肉鸡的胸腺指数的趋势(P=0.076)。2)PT100组42日龄肉鸡的血浆过氧化氢酶(CAT)活性显著高于PT0、PT500和PT1 000组(P<0.05),且血浆CAT活性与化香果单宁添加量呈显著二次曲线相关(P<0.05)。PT0、PT100和PT500组42日龄肉鸡的血浆谷胱甘肽过氧化物酶(GSH-Px)活性显著高于PT1 000组(P<0.05),且血浆GSH-Px活性与化香果单宁添加量呈显著二次曲线相关(P<0.05)。PT100组42日龄肉鸡的血浆总抗氧化能力(T-AOC)显著高于PT0组(P<0.05),且血浆T-AOC与化香果单宁添加量呈显著线性和二次曲线相关(P<0.05)。3)PT100、PT500和PT1 000组42日龄肉鸡的血浆肌酐(CREA)含量显著低于PT0组(P<0.05),且血浆CREA含量与化香果单宁添加量呈显著线性和二次曲线相关(P<0.05)。PT100和PT500组21日龄肉鸡的血浆高密度脂蛋白(HDL)含量显著高于PT0组(P<0.05)。饲粮添加化香果单宁具有降低42日龄肉鸡的血浆尿素氮(UN)和尿酸(UA)含量的趋势(P=0.096和P=0.070)。4)PT100组的盲肠坦纳菌科(Tannerellaceae)相对丰度显著高于PT0组(P<0.05),盲肠副拟杆菌属(Parabacteroides)相对丰度显著高于PT0组(P<0.05)。由此可见,饲粮添加化香果单宁能够增加肉鸡盲肠Parabacteroides相对丰度,提高抗氧化功能,改善血浆生化指标,并且有利于肉鸡免疫器官发育。

本文引用格式

从光雷 , 肖蕴祺 , 张倩雲 , 张雪 , 张倚剑 , 邵丹 , 张安 , 施寿荣 . 饲粮添加化香果单宁对肉鸡免疫器官指数、血浆抗氧化和生化指标及盲肠微生物区系的影响[J]. 动物营养学报, 2021 , 33(12) : 6802 -6812 . DOI: 10.3969/j.issn.1006-267x.2021.12.022

Abstract

This experiment was conducted to study the effects of dietary Plotytarya strohilacea Sieb. et Zuce tannin on immune organ indexes, plasma antioxidant and biochemical indexes and cecal microflora of broilers. A total of 288 one-day-old male Arbor Acres (AA) broilers were randomly divided into 4 groups with 6 replicates in each group and 12 birds in each replicate. Broilers in the control group (PT0 group) were fed a basal diet, and the others in experimental groups were fed basal diets supplemented with 100 (PT100 group), 500 (PT500 group) and 1 000 mg/kg (PT1 000 group) Plotytarya strohilacea Sieb. et Zuce tannin, respectively. The experiment lasted for 42 days. The results showed as follows:1) the bursa of Fabricius index of broilers at 21 days of age of PT500 and PT1 000 groups was significantly higher than that of PT0 and PT100 groups (P<0.05). Dietary Plotytarya strohilacea Sieb. et Zuce tannin had a tendency to increase the thymus index of broilers at 21 days of age (P=0.076). 2) The plasma catalase (CAT) activity of broilers at 42 days of age of PT100 group was significantly higher than that of PT0, PT500 and PT1 000 groups (P<0.05), and there was a significant quadratic curve correlation between the plasma CAT activity and Plotytarya strohilacea Sieb. et Zuce tannin addition amount (P<0.05). The plasma glutathione peroxidase (GSH-Px) activity of broilers at 42 days of age of PT0, PT100 and PT500 groups was significantly higher than that of PT1 000 group (P<0.05), and there was a significant quadratic curve correlation between the plasma GSH-Px activity and Plotytarya strohilacea Sieb. et Zuce tannin addition amount (P<0.05). The plasma total antioxidant capacity (T-AOC) of broilers at 42 days of age of PT100 group was significantly higher than that of PT0 group (P<0.05), and there were significant linear and quadratic curve correlations between the plasma T-AOC and Plotytarya strohilacea Sieb. et Zuce tannin addition amount (P<0.05). 3) The plasma creatinine (CREA) content of broilers at 42 days of age of PT100, PT500 and PT1 000 groups was significantly lower than that of PT0 group (P<0.05), and there was a significant quadratic curve correlation between the plasma CREA content and Plotytarya strohilacea Sieb. et Zuce tannin addition amount (P<0.05). The plasma high-density lipoprotein (HDL) content of broilers at 21 days of age of PT100 and PT500 groups was significantly higher than that of PT0 group (P<0.05). Dietary Plotytarya strohilacea Sieb. et Zuce tannin had a tendency to reduce the plasma urea nitrogen (UN) and uric acid (UA) contents of broilers at 42 days of age (P=0.096 and P=0.070). 4) The cecal Tannerellaceae relative abundance of PT100 group was significantly higher than that of PT0 group (P<0.05), and the cecal Parabacteroides relative abundance was significantly higher than that of PT0 group (P<0.05). In conclusion, dietary Plotytarya strohilacea Sieb. et Zuce tannin can increase the cecal Parabacteroides relative abundance of broilers, improve antioxidant function, improve plasma biochemical indexes, and is beneficial to the immune organ development of broilers.

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