动物营养学报 >

2021 , Vol. 33 >Issue 12: 6853 - 6863

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.12.027

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

胍基乙酸对肉牛生长性能、血浆抗氧化和糖代谢指标及血液相关基因表达的影响

  • 王子苑 ,
  • 陈光吉 ,
  • 舒健虹 ,
  • 张蓉 ,
  • 马广智 ,
  • 何廷章 ,
  • 王小利
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  • 1. 贵州省农业科学院草业研究所, 贵阳 550006;
    2. 安徽万合佳尔生物科技有限公司, 滁州 239000;
    3. 绥阳县枧坝镇农业服务中心, 绥阳 563307
王子苑(1990-),女,贵州遵义人,助理研究员,硕士,从事反刍动物营养与饲料研究。E-mail:wangziyuan525@163.com

收稿日期: 2021-04-28

  网络出版日期: 2021-12-16

基金资助

贵州省农业科学院基金项目（黔农科院青年基金[2018]50号）；贵州省科技创新人才团队（黔科合平台人才[2020]5005）

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Effects of Guanidine Acetic Acid on Growth Performance, Plasma Antioxidant and Glycometabolism Indexes and Blood Related Genes Expression of Beef Cattle

  • WANG Ziyuan ,
  • CHEN Guangji ,
  • SHU Jianhong ,
  • ZHANG Rong ,
  • MA Guangzhi ,
  • HE Tingzhang ,
  • WANG Xiaoli
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  • 1. Guizhou Institute of Prataculture, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;
    2. Anhui Wanhejiaer Biological Technology Co., Ltd., Chuzhou 239000, China;
    3. Agricultural Service Center of Jianba Town of Suiyang County, Suiyang 563307, China

Received date: 2021-04-28

  Online published: 2021-12-16

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摘要

本试验旨在研究胍基乙酸对肉牛生长性能、血浆抗氧化和糖代谢指标及血液相关基因表达的影响。选取平均体重为（473.58±64.40）kg的健康西门塔尔牛24头，随机分为3组，每组8个重复，每个重复1头牛。对照组饲喂基础饲粮，试验组分别在基础饲粮中添加400（G1组）和800 mg/kg（G2组）的胍基乙酸。预试期7 d，正试期49 d。结果表明：1）与对照组相比，G2组的平均日增重显著升高（P<0.05），料重比显著下降（P<0.05）。各组间的末重和平均日采食量无显著差异（P>0.05）。2）与对照组相比，G1组和G2组的血浆总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）活性显著升高（P<0.05），血浆过氧化氢酶（CAT）活性极显著升高（P<0.01），血浆丙二醛（MDA）含量极显著降低（P<0.01）。各组间血浆超氧化物歧化酶（SOD）活性无显著差异（P>0.05）。3）与对照组相比，G1组和G2组的血浆葡萄糖-6-磷酸脱氢酶（G-6-PDH）活性极显著降低（P<0.01），血浆丙酮酸激酶（PK）活性显著降低（P<0.05）；G2组的血浆磷酸果糖激酶（PFK）活性显著降低（P<0.05）；G1组和G2组的血浆肌酸激酶（CK）活性显著升高（P<0.05）。各组间血浆肌酐（CRE）含量无显著差异（P>0.05）。4）与对照组相比，G1组和G2组血液中肌酸转运蛋白（CrT）、腺苷酸活化蛋白激酶（AMPK）和γ-氨基丁酸转运蛋白2（GAT2）mRNA相对表达量均极显著降低（P<0.01）。由此可见，饲粮中添加胍基乙酸能下调血液中CrT、AMPK、GAT2的mRNA相对表达量，降低糖类分解代谢，提升机体抗氧化能力，从而提高肉牛生长性能。

关键词： 胍基乙酸; 肉牛; 生长性能; 抗氧化能力; 糖代谢; 基因表达

本文引用格式

王子苑 , 陈光吉 , 舒健虹 , 张蓉 , 马广智 , 何廷章 , 王小利 . 胍基乙酸对肉牛生长性能、血浆抗氧化和糖代谢指标及血液相关基因表达的影响[J]. 动物营养学报, 2021 , 33(12) : 6853 -6863 . DOI: 10.3969/j.issn.1006-267x.2021.12.027

Abstract

This experiment was conducted to investigate the effects of guanidine acetic acid on growth performance, plasma antioxidant and glycometabolism indexes and blood related genes expression of beef cattle. Twenty-four healthy Simmental beef cattle with an average weight of (473.58±64.40) kg were selected and randomly divided into three groups with eight replicates per group and one cattle per replicate. The cattle in the control group were fed a basal diet, and the others in experimental groups were fed the basal diets supplemented with 400 (G1 group) and 800 mg/kg (G2 group) guanidine acetic acid, respectively. The pretest lasted for 7 days,and the experimental period lasted for 49 days. The results showed as follows:1) compared with the control group, the average daily gain of G2 group was significantly increased (P<0.05), and the feed to gain ratio was significantly decreased (P<0.05). There were no significant differences in final weight and average daily feed intake among all groups (P>0.05). 2) Compared with the control group, the total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) activity in plasma of G1 group and G2 group were significantly increased (P<0.05), the plasma catalase (CAT) activity was significantly increased (P<0.01), and the plasma malondialdehyde (MDA) content was significantly decreased (P<0.01). There was no significant difference in plasma superoxide dismutase (SOD) activity among all groups (P>0.05). 3) Compared with the control group, the plasma glucose-6-phosphate dehydrogenase (G-6-PDH) activity of G1 group and G2 group was significantly decreased (P<0.01), and the plasma pyruvate kinase (PK) activity was significantly decreased (P<0.05); the plasma phosphofructokinase (PFK) activity of G2 group was significantly decreased (P<0.05); the plasma creatine kinase (CK) activity of G1 group and G2 group was significantly increased (P<0.05). There was no significant difference in plasma creatinine (CRE) content among all groups (P>0.05). 4) Compared with the control group, the mRNA relative expression levels of creatine transporter (CrT), adenylate activated protein kinase (AMPK) and γ-aminobutyric acid transporter 2 (GAT2) in blood of G1 group and G2 group were significantly decreased (P<0.01). In summary, the addition of guanidine acetic acid in diets can down-regulate the mRNA expression of CrT, AMPK and GAT2 in blood, reduce catabolism of carbohydrate, and enhance the body antioxidant capacity, thereby improve the growth performance of beef cattle.

Key words： guanidine acetic acid; beef cattle; growth performance; antioxidant capacity; glycometabolism; gene expression

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