水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

饲料中添加黑水虻幼虫粉对加州鲈血清代谢物、抗氧化与免疫指标及肠道组织结构的影响

  • 彭凯 ,
  • 萧鸿发 ,
  • 莫文艳 ,
  • 王国霞 ,
  • 黄燕华
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  • 1. 广东省农业科学院动物科学研究所, 广东省畜禽育种与营养研究重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广州 510640;
    2. 广州飞禧特生物科技有限公司, 广州 510640;
    3. 仲恺农业工程学院健康养殖创新研究院, 广州 510225
彭凯(1987-),男,河南信阳人,副研究员,博士,从事水产动物营养与饲料研究。E-mail:pengkai1016@126.com

收稿日期: 2021-04-27

  网络出版日期: 2021-12-16

基金资助

广东省现代农业产业技术体系创新团队建设项目(2019KJ115);科技创新战略专项(高水平农科院建设)(R2018QD-075)

Effects of Dietary Black Soldier Fly Larvae Meal on Serum Metabolites, Antioxidant and Immune Indexes and Intestinal Histological Appearance of Micropterus salmoides

  • PENG Kai ,
  • XIAO Hongfa ,
  • MO Wenyan ,
  • WANG Guoxia ,
  • HUANG Yanhua
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  • 1. Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture in Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Guangzhou Fishtech Biotechnology Co., Ltd., Guangzhou 510640, China;
    3. Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

Received date: 2021-04-27

  Online published: 2021-12-16

摘要

本试验旨在研究饲料中添加黑水虻幼虫粉替代鱼粉对加州鲈血清代谢物、抗氧化与免疫指标和肠道组织结构的影响。选择初始体重为(4.9±0.1)g的加州鲈鱼苗525尾,随机分为5组,每组3个重复,每个重复35尾。以黑水虻幼虫粉替代鱼粉,分别投喂G0(基础饲料,鱼粉添加比例为45.0%)、G7.5(替代鱼粉比例为7.5%)、G15.0(替代鱼粉比例为15.0%)、G22.5(替代鱼粉比例为22.5%)和G30.0(替代鱼粉比例为30.0%)5种等氮等脂饲料,养殖周期为62 d。结果表明:与G0组相比,G15.0~G30.0组血清白蛋白含量显著升高(P<0.05);G22.5和G30.0组血清球蛋白含量显著升高(P<0.05),胆固醇含量和谷草转氨酶活性显著降低(P<0.05);G30.0组血清高密度脂蛋白胆固醇含量显著增加(P<0.05);G7.5~G30.0组血清谷胱甘肽过氧化物酶活性和补体3含量显著增加(P<0.05);G15.0~G30.0组血清酸性磷酸酶活性显著升高(P<0.05);G7.5~G30.0组肠道组织有不同程度损伤,且随着黑水虻幼虫粉替代鱼粉比例越高,肠道组织损伤越严重。综上所述,饲料中添加黑水虻幼虫粉替代鱼粉提高了加州血清抗氧化和免疫指标,但不同程度地损伤了加州鲈肝脏功能和肠道组织结构,建议饲料中应当谨慎添加。

本文引用格式

彭凯 , 萧鸿发 , 莫文艳 , 王国霞 , 黄燕华 . 饲料中添加黑水虻幼虫粉对加州鲈血清代谢物、抗氧化与免疫指标及肠道组织结构的影响[J]. 动物营养学报, 2021 , 33(12) : 6964 -6972 . DOI: 10.3969/j.issn.1006-267x.2021.12.037

Abstract

This experiment was aimed to study the effects of dietary black soldier fly larvae meal replacing fish meal on serum metabolites, antioxidant and immune indexes and intestinal histological appearance of Micropterus salmoides. A total of 525 juvenile Micropterus salmoides with an initial body weight of (4.9±0.1) g were divided into 5 groups with 3 replicates in each group and 35 fish in each replicate. Five isoproteic and isolipidic diets were formulated by adding black soldier fly larvae meal to replace 0 (G0), 7.5% (G7.5), 15.0% (G15.0), 22.5% (G22.5) and 30.0% (G30.0) of fish meal in diets. The feeding period was 62 days. Results showed as follows:compared with G0 group, serum albumin content in G15.0 to G30.0 groups was significantly increased (P<0.05), and serum globulin content in G22.5 and G30.0 groups was significantly increased (P<0.05), but cholesterol content and aspartate aminotransferase activity were significantly decreased (P<0.05); serum high density lipoprotein cholesterol content in G30.0 group were significantly increased (P<0.05);serum glutathion peroxidase activity and complement C3 content in G7.5 to G30.0 groups was significantly increased (P<0.05), and serum acid phosphatase activity in G15.0 to G30.0 groups was significantly increased (P<0.05). The intestine tissue in G7.5 to G30.0 groups had varying degrees of injury, and the higher replacement ratio of larvae meal to fish meal, the more serious damage of intestine tissue was. In conclusion, black soldier fly larvae meal replacing fish meal in diets significantly improves serum antioxidant and immunity, but damages liver function and intestinal histological appearance of Micropterus salmoides. Therefore, it is suggested that dietary supplementation of black soldier fly larvae meal should be cautious.

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