水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

饲料中添加甘露寡糖对珍珠龙胆石斑鱼生长性能、血清免疫指标、转录组及肠道菌群的影响

  • 王红明 ,
  • 丁雪婧 ,
  • 陈俭 ,
  • 宋守钢 ,
  • 谭北平 ,
  • 章双
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  • 1. 广东海洋大学水产动物营养与饲料实验室, 湛江 524088;
    2. 农业部华南水产与畜禽饲料重点实验室, 湛江 524088
王红明(1997-),男,四川大竹人,硕士研究生,从事水产动物营养与饲料学研究。E-mail:631255674@qq.com

收稿日期: 2021-04-27

  网络出版日期: 2021-12-16

基金资助

国家重点研发计划项目(2019YFD0900200)

Effects of Dietary Mannan-Oligosaccharides on Growth Performance, Serum Immune Indices, Transcriptome and Intestinal Microflora of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂

  • WANG Hongming ,
  • DING Xuejing ,
  • CHEN Jian ,
  • SONG Shougang ,
  • TAN Beiping ,
  • ZHANG Shuang
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  • 1. Laboratory of Aquatic Animal Nutrition and Feeding, Guangdong Ocean University, Zhanjiang 524088, China;
    2. Key Laboratory of Aquatic and Livestock Feed in South China, Ministry of Agriculture, Zhanjiang 524088, China

Received date: 2021-04-27

  Online published: 2021-12-16

摘要

本试验旨在研究饲料中添加甘露寡糖对珍珠龙胆石斑鱼(Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂)生长性能、血清免疫指标、转录组及肠道菌群的影响。选择240尾初始平均体重为(6.00±0.32)g的珍珠龙胆石斑鱼幼鱼,随机分为2组,每组3个重复,每个重复40尾鱼。对照组饲喂基础饲料,甘露寡糖组在基础饲料中添加100 mg/kg甘露寡糖。养殖8周后,分析甘露寡糖对珍珠龙胆石斑鱼生长性能、血清免疫指标、转录组和肠道菌群的影响。结果显示:1)甘露寡糖组的终末体重、增重率、特定生长率和肥满度均显著高于对照组(P<0.05)。2)甘露寡糖组的血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、溶菌酶(LZM)和碱性磷酸酶(AKP)活性显著高于对照组(P<0.05),血清丙二醛(MDA)含量显著低于对照组(P<0.05)。3)转录组测序分析共鉴定出9 964个差异表达基因,其中3 801个表达上调,6 163个表达下调。GO功能富集分析结果表明,差异表达基因在生物过程、细胞组成和分子功能显著富集。KEEG通路富集分析发现,差异表达基因在吞噬体、细胞黏附分子、细胞因子-细胞因子受体相互作用、免疫球蛋白A产生的肠道免疫网络相关通路中显著富集。4)甘露寡糖组肠道菌群的操作分类单元(OTU)数量、Ace指数、Chao1指数和Shannon指数均显著高于对照组(P<0.05)。甘露寡糖组肠道拟杆菌门和厚壁菌门相对丰度显著高于对照组(P<0.05)。甘露寡糖组肠道发光杆菌属相对丰度显著低于对照组(P<0.05),而肠道德沃斯氏菌属、盐单胞菌属和甲基杆菌属相对丰度显著高于对照组(P<0.05)。综上所述,饲料中添加100 mg/kg甘露寡糖可促进珍珠龙胆石斑鱼的生长,提高免疫功能,改善肠道菌群结构。

本文引用格式

王红明 , 丁雪婧 , 陈俭 , 宋守钢 , 谭北平 , 章双 . 饲料中添加甘露寡糖对珍珠龙胆石斑鱼生长性能、血清免疫指标、转录组及肠道菌群的影响[J]. 动物营养学报, 2021 , 33(12) : 6982 -6998 . DOI: 10.3969/j.issn.1006-267x.2021.12.039

Abstract

This experiment was conducted to study the effects of dietary mannan-oligosaccharides on growth performance, serum immune indices, transcriptome and intestinal microflora of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂. A total of 240 juvenile Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂ with initial average body weight of (6.00±0.32) g were randomly divided into 2 groups with 3 replicates per group and 40 fish per replicate. Fish in the control group were fed a basal diet, and others in mannan-oligosaccharides group were fed the basal diet supplemented with 100 mg/kg mannan-oligosaccharides. After 8 weeks breeding, the effects of dietary mannan-oligosaccharides on growth performance, serum immune indices, transcriptome and intestinal microflora of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂ were analyzed. The results showed as follows:1) the final body weight, weight gain rate, specific growth rate and condition factor of mannan-oligosaccharides group were significantly higher than those of the control group (P<0.05). 2) The activities of superoxide dismutase (SOD), catalase (CAT), lysozyme (LZM) and alkaline phosphatase (AKP) in serum of mannan-oligosaccharides group were significantly higher than those of the control group (P<0.05), while the serum malondialdehyde (MDA) content was significantly lower than that of the control group (P<0.05). 3) Transcriptome sequencing analysis identified 9 964 differentially expressed genes, which 3 801 were up-regulated and 6 163 were down-regulated. GO functional enrichment analysis revealed that the differentially expressed genes were significantly enriched in biological process, molecular function and cell component. KEEG pathway enrichment analysis differentially expressed genes in phagosome, cell adhesion molecules, cytokine-cytokine receptor interactions and immunoglobulin A-producing intestinal immune network related pathways were significantly enriched. 4) The operational taxonomic unit (OTU) number, Ace index, Chao1 index and Shannon index of intestinal microflora of mannan-oligosaccharides group were significantly higher than those of the control group (P<0.05). The relative abundances of Bacteroidetes and Firmicutes in intestine of mannan-oligosaccharides group were significantly higher than those of the control group (P<0.05). The relative abundances of Photobacterium in intestine of mannan-oligosaccharides group was significantly lower than that of the control group (P<0.05), and the relative abundances of Devosia, Halomonas and Methylobacterium in intestine were significantly higher than those of the control group (P<0.05). In conclusion, dietary 100 mg/kg mannan-oligosaccharides can significantly promote the growth performance of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂, improve the immune function, and improve the intestinal microflora structure.

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