动物营养学报 >

2022 , Vol. 34 >Issue 2: 908 - 923

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.02.024

研究论文 RESEARCH PAPER

加工工艺和湿态发酵豆粕添加水平对肉鸡颗粒饲料质量、生长性能和抗氧化能力的影响

  • 鲁春灵 ,
  • 秦玉昌 ,
  • 李俊 ,
  • 谷旭 ,
  • 张羽 ,
  • 董颖超 ,
  • 牛力斌 ,
  • 商方方 ,
  • 杨洁 ,
  • 李军国
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  • 1. 中国农业科学院饲料研究所, 北京 100081;
    2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
    3. 中国农业科学技术出版社, 北京 100081
鲁春灵(1996-),女,河北秦皇岛人,硕士研究生,研究方向为动物营养与饲料加工。E-mail:656260445@qq.com

收稿日期: 2021-07-18

  网络出版日期: 2022-02-15

基金资助

现代农业产业技术体系北京市家禽创新团队项目（BAIC04-2021）；国家重点研发计划项目（2018YFD0500600）；中国农业科学院创新工程项目（CAAS-ASTIP-2021-FRI-08）

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Effects of Processing Technology and Wet Fermented Soybean Meal Supplemental Levels on Pellet Feed Quality, Growth Performance and Antioxidant Capability of Broilers

  • LU Chunling ,
  • QIN Yuchang ,
  • LI Jun ,
  • GU Xu ,
  • ZHANG Yu ,
  • DONG Yingchao ,
  • NIU Libin ,
  • SHANG Fangfang ,
  • YANG Jie ,
  • LI Junguo
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  • 1. Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. China Agricultural Science and Technology Press, Beijing 100081, China

Received date: 2021-07-18

  Online published: 2022-02-15

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摘要

本试验旨在研究加工工艺和湿态发酵豆粕添加水平及其交互作用对肉鸡颗粒饲料质量、生长性能、抗氧化能力以及肠道组织形态的影响。采用2×3双因素试验设计，加工工艺为普通调质制粒（NCP）工艺和高温调质低温制粒（HCLP）工艺；湿态发酵豆粕的添加水平为0、5%和10%。试验选取1日龄爱拔益加（AA）肉仔鸡480只，按照体重相近原则随机分为6个组，每组8个重复，每个重复10只鸡（公母各占1/2）。试验期42 d，分为前期（1~21日龄）和后期（22~42日龄）2个阶段。结果表明：1）与NCP工艺相比，HCLP工艺显著提高肉鸡前期料和后期料的淀粉糊化度、颗粒耐久性（PDI）和颗粒硬度（P<0.05）；随着饲粮湿态发酵豆粕添加水平的提高，肉鸡后期料PDI显著提高（P<0.05）；加工工艺和湿态发酵豆粕添加水平对肉鸡前期料颗粒硬度以及后期料淀粉糊化度和PDI有显著交互作用（P<0.05）。2）与NCP工艺相比，HCLP工艺显著提高肉鸡1~21日龄平均日增重（P<0.05）；肉鸡1~21日龄、22~42日龄和1~42日龄料重比随着饲粮湿态发酵豆粕添加水平的提高而显著降低（P<0.05）；加工工艺和湿态发酵豆粕添加水平对肉鸡1~21日龄平均日采食量有显著交互作用（P<0.05）。3）与NCP工艺相比，HCLP工艺显著降低肉鸡胸肌红度值（P<0.05）；加工工艺和湿态发酵豆粕添加水平对腿肌亮度值有显著交互作用（P<0.05）。4）随着饲粮湿态发酵豆粕添加水平的提高，肉鸡血清超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性显著提高（P<0.05），而血清丙二醛含量显著降低（P<0.05），表明湿态发酵豆粕能提高肉鸡抗氧化能力，且加工工艺和湿态发酵豆粕添加水平对肉鸡抗氧化能力有显著交互作用（P<0.05）。5）与NCP工艺相比，HCLP工艺显著提高肉鸡空肠和回肠绒毛高度和绒毛高度/隐窝深度（V/C）值（P<0.05），显著降低十二指肠隐窝深度（P<0.05），显著提高十二指肠V/C值（P<0.05）。随着饲粮湿态发酵豆粕添加水平的提高，肉鸡十二指肠、空肠和回肠绒毛高度和V/C值显著提高（P<0.05），隐窝深度显著降低（P<0.05）。加工工艺和湿态发酵豆粕添加水平对十二指肠和空肠隐窝深度和V/C值以及空肠和回肠绒毛高度有显著交互作用（P<0.05）。综上所述，在HCLP工艺下，饲粮添加5%~10%的湿态发酵豆粕可以提高肉鸡颗粒质量、生长性能和抗氧化能力，改善肠道组织形态。

关键词： 加工工艺; 饲料颗粒质量; 湿态发酵豆粕; 肉鸡; 生长性能; 抗氧化能力

本文引用格式

鲁春灵 , 秦玉昌 , 李俊 , 谷旭 , 张羽 , 董颖超 , 牛力斌 , 商方方 , 杨洁 , 李军国 . 加工工艺和湿态发酵豆粕添加水平对肉鸡颗粒饲料质量、生长性能和抗氧化能力的影响[J]. 动物营养学报, 2022 , 34(2) : 908 -923 . DOI: 10.3969/j.issn.1006-267x.2022.02.024

Abstract

This experiment was conducted to study the effects of processing technology and wet fermented soybean meal supplemental levels, and their interaction on pellet feed quality, growth performance, antioxidant capacity and intestinal morphology of broilers. A 2×3 two-factor experimental design was adopted. Two processing technology methods were normal conditioning and pelleting (NCP) and high-temperature conditioning and low-temperature pelleting (HCLP). The supplemental levels of wet fermented soybean meal were 0, 5% and 10%. A total of 480 Arbor Acres (AA) broilers of one-day-old were randomly divided into 6 groups with 8 replicates per group and 10 broilers per replicate (half male and half female) according to the principle of similar body weight. The experiment lasted for 42 days and was divided into two stages as early stage (1 to 21 days of age) and late stage (22 to 42 days of age). The results showed as follows:1) compared with NCP technology, HCLP technology significantly increased the starch gelatinization degree, pellet durability index (PDI) and pellet hardness of the early and late feed for broilers (P<0.05). The PDI of the late feed for broilers was significantly increased with the increase of wet fermented soybean meal supplemental level (P<0.05). The interaction between processing technology and wet fermented soybean meal supplemental level had a significant effect on the pellet hardness of the early feed and starch gelatinization degree and PDI of the late feed for broilers (P<0.05). 2) Compared with NCP technology, HCLP technology significantly increased the average daily gain of broilers from 1 to 21 days of age (P<0.05). The feed to gain ratio (F/G) of broilers from 1 to 21, 22 to 42 and 1 to 42 days of age was significantly decreased with the increase of wet fermented soybean meal supplemental level (P<0.05). The interaction between processing technology and wet fermented soybean meal supplemental level had a significant effect on the average daily feed gain of broilers from 1 to 21 days of age (P<0.05). 3) Compared with NCP technology, HCLP technology significantly reduced the redness in breast muscle of broilers (P<0.05). The interaction between processing technology and wet fermented soybean meal supplemental level had a significant effect on the lightness in leg muscle of broilers (P<0.05). 4) The serum activities of superoxide dismutase, glutathione peroxidase and catalase of broilers were significantly increased (P<0.05), while the serum malondialdehyde content was significantly decreased with the increase of wet fermented soybean meal level (P<0.05), which indicated that wet fermented soybean meal could improve the antioxidant capability of broilers. The interaction between processing technology and wet fermented soybean meal supplemental level had a significant effect on the antioxidant capability of broilers (P<0.05). 5) Compared with NCP technology, HCLP technology significantly increased the villus height and the ratio of villus height to crypt depth (V/C) in jejunum and ileum of broilers (P<0.05), and significantly decreased the crypt depth and increased the V/C in duodenum (P<0.05). The villus height and V/C in duodenum, jejunum and ileum of broilers were significantly increased (P<0.05), while the crypt depth was significantly decreased with the increase of wet fermented soybean meal level (P<0.05). The crypt depth and V/C in duodenum and jejunum, as well as the villus height in jejunum and ileum, were significantly influenced by the interaction between processing technology and wet fermented soybean meal supplemental level (P<0.05). In conclusion, dietary 5% to 10% wet fermented soybean meal with HCLP technology can improve the pellet feed quality, growth performance, antioxidant capacity and intestinal morphology of broilers.

Key words： processing technology; pellet feed quality; wet fermented soybean meal; broilers; growth performance; antioxidant capability

参考文献

[1] SOOMRO R N, YAO J H, GOLA M A, et al.Beneficial aspects of feeding soybean meal (SBM) fermented with fungal and bacterial actions in broiler chickens[J].American Journal of Biology and Life Sciences, 2017, 5(4):21-29.
[2] CHENG Y H, HSIAO F S H, WEN C M, et al.Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers[J].Journal of Applied Animal Research, 2019, 47(1):339-348.  
[3] 段海涛.高效调质低温制粒畜禽饲料加工工艺及其对生长育肥猪生长性能的影响研究[D].博士学位论文.北京:中国农业科学院, 2018. DUAN H T.Study on the high-efficiency condition and low-temperature pelleted processing technology and effects on growth performance of growing and fattening pigs[D].Ph.D. Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2018.(in Chinese)
[4] National Research Council.Nutrient requirements of poultry[M].9th ed.Washington, D.C.:The National Academies Press, 1994.
[5] 熊易强.饲料淀粉糊化度 (熟化度) 的测定[J].饲料工业, 2000, 21(3):30-31. XIONG Y Q.Determination of starch gelatinization (cooking degree) in feed[J].Feed Industry, 2000, 21(3):30-31.(in Chinese)
[6] 冯幼, 许合金, 刘定, 等.不同因素对水产饲料淀粉糊化度的影响[J].饲料博览, 2014(10):51-54. FENG Y, XU H J, LIU D, et al.Effect of different factors on the starch gelatinization degree of aquatic feeds[J].Feed Review, 2014(10):51-54.(in Chinese)
[7] CUTLIP S E, HOTT J M, BUCHANAN N P, et al.The effect of steam-conditioning practices on pellet quality and growing broiler nutritional value[J].Journal of Applied Poultry Research, 2008, 17(2):249-261.  
[8] 张现玲, 秦玉昌, 李俊, 等.调质温度对肉鸡颗粒饲料质量影响的实验研究[J].饲料工业, 2013, 34(21):24-28. ZHANG X L, QIN Y C, LI J, et al.Experiment research on the effect of conditioning temperature on broiler pellet feed quality[J].Feed Industry, 2013, 34(21):24-28.(in Chinese)
[9] 胡凯飞, 王金荣, 于翠平, 等.饲料的粗脂肪水平和调质温度对颗粒饲料硬度的影响[J].中国畜牧杂志, 2018, 54(5):108-112. HU K F, WANG J R, YU C P, et al.Effect of ether extract and conditioning temperature on the hardness of pellet feed[J].Chinese Journal of Animal Science, 2018, 54(5):108-112.(in Chinese)
[10] MURAMATSU K.Predictive modeling application in pelleting process of the feed for broilers[D].Ph.D. Thesis.Curitiba:Universidade Federal do Paraná, 2013.
[11] 张现玲.调质温度、粉碎粒度对肉鸡颗粒饲料质量及利用率的影响研究[D].硕士学位论文.北京:中国农业科学院, 2013. ZHANG X L.Effect of conditioning temperatures and particle sizes on quality and utilization rate of broiler pellet feed[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2013.(in Chinese)
[12] ABDOLLAHI M R, RAVINDRAN V, WESTER T J, et al.Influence of conditioning temperature on performance, apparent metabolisable energy, ileal digestibility of starch and nitrogen and the quality of pellets, in broiler starters fed maize- and sorghum-based diets[J].Animal Feed Science and Technology, 2010, 162(3/4):106-115.
[13] ABDOLLAHI M R, RAVINDRAN V, WESTER T J, et al.Influence of feed form and conditioning temperature on performance, apparent metabolisable energy and ileal digestibility of starch and nitrogen in broiler starters fed wheat-based diet[J].Animal Feed Science and Technology, 2011, 168(1/2):88-99.
[14] ZAEFARIAN F, ABDOLLAHI M R, RAVINDRAN V.Starch digestion in broiler chickens fed cereal diets[J].Animal Feed Science and Technology, 2015, 209:16-29.
[15] 王昊, 于纪宾, 于治芹, 等.饲料淀粉糊化度对肉鸡生长性能及蛋白质体外消化率的影响[J].饲料工业, 2017, 38(6):35-40. WANG H, YU J B, YU Z Q, et al.Effect of feed starch gelatinization on growth performance and in-vitro protein digestibility of broilers[J].Feed Industry, 2017, 38(6):35-40.(in Chinese)
[16] CHACHAJ R, SEMBRATOWICZ I, KRAUZE M, et al.The effect of partial replacement of soybean meal with fermented soybean meal on chicken performance and immune status[J].Journal of Animal and Feed Sciences, 2019, 28(3):263-271.
[17] LI Y, GUO B Z, WU Z K, et al.Effects of fermented soybean meal supplementation on the growth performance and cecal microbiota community of broiler chickens[J].Animals, 2020, 10(6):1098.
[18] SOUMEH E A, MOHEBODINI H, TOGHYANI M, et al.Synergistic effects of fermented soybean meal and mannan-oligosaccharide on growth performance, digestive functions, and hepatic gene expression in broiler chickens[J].Poultry Science, 2019, 98(12):6797-6807.  
[19] FELLENBERG M A, SPEISKY H.Antioxidants:their effects on broiler oxidative stress and its meat oxidative stability[J].World's Poultry Science Journal, 2006, 62(1):53-70.  
[20] ALIAN H A, SAMY H M, IBRAHIM M T, et al.Nanoselenium effect on growth performance, carcass traits, antioxidant activity, and immune status of broilers[J].Environmental Science and Pollution Research, 2020, 27(31):38607-38616.  
[21] DANESHYAR M.Effect of dietary turmeric on antioxidant properties of thigh meat in broiler chickens after slaughter[J].Animal Science Journal, 2012, 83(8):599-604.  
[22] GUO S S, ZHANG Y K, CHENG Q, et al.Partial substitution of fermented soybean meal for soybean meal influences the carcass traits and meat quality of broiler chickens[J].Animals, 2020, 10(2):225.
[23] LI C Q, ZHANG B L, LIU C D, et al.Effects of dietary raw or Enterococcus faecium fermented soybean meal on growth, antioxidant status, intestinal microbiota, morphology, and inflammatory responses in turbot (Scophthalmus maximus L.)[J].Fish & Shellfish Immunology, 2020, 100:261-271.
[24] CHACHAJ R, SEMBRATOWICZ I, KRAUZE M, et al.The effect of fermented soybean meal on performance, biochemical and immunological blood parameters in turkeys[J].Annals of Animal Science, 2019, 19(4):1035-1049.  
[25] WU P, GOLLY M K, GUO Y T, et al.Effect of partial replacement of soybean meal with high-temperature fermented soybean meal in antibiotic-growth-promoter-free diets on growth performance, organ weights, serum indexes, intestinal flora and histomorphology of broiler chickens[J].Animal Feed Science and Technology, 2020, 269:114616.
[26] SEMBRATOWICZ I, CHACHAJ R, KRAUZE M, et al.The effect of diet with fermented soybean meal on blood metabolites and redox status of chickens[J].Annals of Animal Science, 2020, 20(2):599-611.  
[27] LIU J B, YAN H L, ZHANG Y, et al.Effects of stale maize on growth performance, immunity, intestinal morphology and antioxidant capacity in broilers[J].Asian-Australasian Journal of Animal Sciences, 2020, 33(4):605-614.  
[28] EKIM B, CALIK A, CEYLAN A, et al.Effects of Paenibacillus xylanexedens on growth performance, intestinal histomorphology, intestinal microflora, and immune response in broiler chickens challenged with Escherichia coli K88[J].Poultry Science, 2020, 99(1):214-223.  
[29] ZHAO Y, ZENG D, WANG H S, et al.Dietary probiotic Bacillus licheniformis H2 enhanced growth performance, morphology of small intestine and liver, and antioxidant capacity of broiler chickens against Clostridium perfringens-induced subclinical necrotic enteritis[J].Probiotics and Antimicrobial Proteins, 2020, 12(3):883-895.  
[30] SALEH A A, SHUKRY M, FARRAG F, et al.Effect of feeding wet feed or wet feed fermented by Bacillus licheniformis on growth performance, histopathology and growth and lipid metabolism marker genes in broiler chickens[J].Animals, 2021, 11(1):83.
[31] FENG J, LIU X, XU Z R, et al.Effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers[J].Poultry Science, 2007, 86(6):1149-1154.  
[32] CHIANG G, LU W Q, PIAO X S, et al.Effects of feeding solid-state fermented rapeseed meal on performance, nutrient digestibility, intestinal ecology and intestinal morphology of broiler chickens[J].Asian-Australasian Journal of Animal Sciences, 2010, 23(2):263-271.
[33] JAZI V, MOHEBODINI H, ASHAYERIZADEH A, et al.Fermented soybean meal ameliorates Salmonella typhimurium infection in young broiler chickens[J].Poultry Science, 2019, 98(11):5648-5660.  
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