动物营养学报 >

2022 , Vol. 34 >Issue 2: 951 - 960

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.02.027

研究论文 RESEARCH PAPER

凝结芽孢杆菌BC-HYI改善脂多糖损伤蛋雏鸡肠道作用的研究

  • 胡婷 ,
  • 张华 ,
  • 吴琼 ,
  • 周波 ,
  • 卢天航 ,
  • 崔德凤 ,
  • 张永红
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  • 1. 北京农学院动物科学技术学院, 兽医学(中医药)北京市重点实验室, 北京 102206;
    2. 河北农业大学 动物科技学院, 保定 071000;
    3. 中牧实业股份有限公司, 北京 100070
胡婷(1996-),女,辽宁大连人,硕士研究生,从事动物疫病综合防控与微生物饲料添加剂研制。E-mail:1641314709@qq.com

收稿日期: 2021-07-22

  网络出版日期: 2022-02-15

基金资助

北京农学院校级协同创新团队项目；2018年度新型生产经营主体科技能力提升项目；北京市生猪产业创新团队项目（BAIC02）；2020年度北京市教育委员会科技计划一般项目（KM202010020009）

收起

Bacillus coagulans BC-HYI Improving Lipopolysaccharide Induced Intestinal Injury in Layer Chickens

  • HU Ting ,
  • ZHANG Hua ,
  • WU Qiong ,
  • ZHOU Bo ,
  • LU Tianhang ,
  • CUI Defeng ,
  • ZHANG Yonghong
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  • 1. Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;
    3. China Animal Husbandry Industry Co., Ltd., Beijing 100070, China

Received date: 2021-07-22

  Online published: 2022-02-15

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摘要

本试验旨在探究凝结芽孢杆菌BC-HYI调节脂多糖（LPS）损伤蛋雏鸡肠道作用。选取1日龄京红蛋雏鸡180只，随机分为5组，每组6个重复，每个重复6只，重复之间体重接近。空白对照组（CON组）和模型组（LPS组）饲喂基础饲粮，连续灌服生理盐水；3个益生菌组饲喂基础饲粮，同时分别连续灌服低、中、高剂量的凝结芽孢杆菌BC-HYI （浓度分别为106、107、108 CFU/mL），连续灌服28 d，28 d后灌服LPS，灌服浓度为2 mg/kg，灌服剂量为200 μL/只，试验周期为6 h，分别记为LOW+LPS、MID+LPS、HIGH+LPS组。6 h后采血，剖检、收集蛋雏鸡十二指肠、空肠、回肠肠道组织及空肠黏膜。结果表明：与空白对照组相比，LPS灌服后，蛋雏鸡十二指肠、空肠、回肠的绒隐比以及空肠黏膜黏液蛋白2（MUC2）、闭合蛋白（Occludin）和闭锁小带蛋白-1（ZO-1） mRNA相对表达量显著降低（P<0.05），血清二胺氧化酶（DAO）活性及D-乳酸（D-Lac）、肿瘤坏死因子-α（TNF-α）含量，空肠黏膜分泌型免疫球蛋白A （SIgA）和白细胞介素-10（IL-10）的含量，空肠黏膜Toll样受体4（TLR4）和核因子-κB （NF-κB）的mRNA相对表达量和蛋白表达量显著提高（P<0.05）。与LPS组相比，中、高剂量凝结芽孢杆菌BC-HYI极显著上调十二指肠、空肠、回肠绒隐比（P<0.01），极显著下调空肠黏膜TLR4和NF-κB的mRNA相对表达量和蛋白表达量（P<0.01），显著下调蛋雏鸡血清DAO活性及D-Lac和TNF-α含量（P<0.05）；凝结芽孢杆菌BC-HYI显著提高空肠黏膜MUC2、Occludin和ZO-1的mRNA相对表达量（P<0.05）；凝结芽孢杆菌BC-HYI对空肠黏膜SIgA和IL-10含量无显著影响（P>0.05）。综上所述，凝结芽孢杆菌BC-HYI对LPS损伤有缓解作用，能降低肠道通透性，下调TLR4/NF-κB信号通路中TLR4和NF-κB的mRNA相对表达量和蛋白表达量，从而改善LPS灌服后的肠道损伤，提高蛋雏鸡肠道健康。

关键词： 凝结芽孢杆菌BC-HYI; 蛋雏鸡; LPS; 肠道黏膜通透性; TLR4信号通路

本文引用格式

胡婷 , 张华 , 吴琼 , 周波 , 卢天航 , 崔德凤 , 张永红 . 凝结芽孢杆菌BC-HYI改善脂多糖损伤蛋雏鸡肠道作用的研究[J]. 动物营养学报, 2022 , 34(2) : 951 -960 . DOI: 10.3969/j.issn.1006-267x.2022.02.027

Abstract

The aim of this study was to explore Bacillus coagulans BC-HYI improving lipopolysaccharide (LPS) induced intestinal injury in layer chickens. The 180 one-day-old Jinghong layer chickens were randomly divided into 5 groups with 6 replicates in each group and 6 layer chickens in each replicate. The weight between replicates was close. The blank control group (CON group) and the model group (LPS group) were fed a basic diet and continuously perfused with normal saline. The three probiotic groups were fed the basic diet and continuously perfused with 106, 107 and 108 CFU/mL Bacillus coagulans BC-HYI, respectively for 28 days. After 28 days, LPS was perfused with a concentration of 2 mg/kg and a dose of 200 μL/chick, the test period was 6 h, and they were recorded as LOW+LPS, MID+LPS and HIGH+LPS groups, respectively. After 6 h, blood was collected, and the duodenum, jejunum, ileum and jejunal mucosa were collected. The results showed that compared with the CON group, after LPS administration, the ratio of villus length to crypt depth (V/C) in intestine of duodenum, jejunum and ileum and the mRNA relative expression levels of mucin 2 (MUC2), Occludin and zonula occludens protein-1 (ZO-1) in jejunal mucosa decreased significantly (P<0.05); and serum diamine oxidase (DAO) activity, D-lactic acid (D-Lac) and tumor necrosis factor-α (TNF-α) contents, jejunal mucosal secretory immunoglobulin A (SIgA) and interleukin-10 (IL-10) contents, the mRNA relative expression levels and protein expression levels of Toll like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) in jejunal mucosal significantly increased (P<0.05). Compared with LPS group, medium and high doses of Bacillus coagulans BC-HYI significantly increased the V/C in intestine of duodenum, jejunum and ileum (P<0.01), and the mRNA relative expression levels and protein expression levels of TLR4 and NF-κB in jejunal mucosa significantly decreased (P<0.01), and the DAO activity and D-Lac content in serum significantly decreased (P<0.05); Bacillus coagulans BC-HYI significantly increased the relative mRNA expression levels of MUC2, Occludin and ZO-1 in jejunal mucosa (P<0.05); there was no significant difference in the contents of SIgA and IL-10 in jejunal mucosa (P>0.05). In conclusion, Bacillus coagulans BC-HYI can alleviate LPS injury, reduce intestinal permeability and down regulate the expression of gene and protein of TLR4 and NF-κB in TLR4/NF-κB signaling pathway, so as to improve the intestinal injury after LPS administration and improve the intestinal health of layer chickens.

Key words： Bacillus coagulans BC-HYI; layer chickens; LPS; intestinal mucosal permeability; TLR4 signaling pathway

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