研究论文 RESEARCH PAPER

饲粮添加发酵枣粉对高温季节荷斯坦公牛生长性能、免疫性能和抗氧化性能的影响

  • 张喆萍 ,
  • 李瑞银 ,
  • 李树静 ,
  • 李建国 ,
  • 沈宜钊 ,
  • 高艳霞 ,
  • 李妍 ,
  • 范京惠 ,
  • 谢鹏 ,
  • 李秋凤 ,
  • 曹玉凤
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  • 1. 河北农业大学动物科技学院, 保定 071000;
    2. 河北天和肉牛养殖有限公司, 石家庄 050200;
    3. 河北农业大学动物医学院, 保定 071000;
    4. 中国农业科学院北京畜牧兽医研究所, 北京 100081
张喆萍(1995-),女,河北廊坊人,硕士研究生,研究方向为反刍动物营养。E-mail:469174869@qq.com

收稿日期: 2021-06-29

  网络出版日期: 2022-02-15

基金资助

河北省二期现代农业产业技术体系肉牛创新团队高效养殖岗位建设项目(HBCT2018130202);国家现代农业产业技术体系(CARS-37);国家重点研发计划(2018YDF0501805)

Effects of Dietary Fermented Jujube Powder on Growth Performance, Immune Performance and Antioxidant Performance of Holstein Bulls during Hot Season

  • ZHANG Zheping ,
  • LI Ruiyin ,
  • LI Shujing ,
  • LI Jianguo ,
  • SHEN Yizhao ,
  • GAO Yanxia ,
  • LI Yan ,
  • FAN Jinghui ,
  • XIE Peng ,
  • LI Qiufeng ,
  • CAO Yufeng
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  • 1. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China;
    2. Hebei Tianhe Beef Cattle Breeding Co., Ltd., Shijiazhuang 050200, China;
    3. College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China;
    4. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2021-06-29

  Online published: 2022-02-15

摘要

本试验旨在研究饲粮添加发酵枣粉对高温季节荷斯坦公牛行为、生长性能、抗氧化性能、免疫性能的影响。试验选取60头4~6月龄体重[(200.00±30.28) kg]相近的健康荷斯坦公牛,随机分成4组,每组15头,分别饲喂含0(C组)、5%(L组)、10%(M组)、15%(H组)发酵枣粉的等能等氮的饲粮。预试期7 d,正试期90 d。结果表明:1)当温湿度指数(THI)<78时,添加发酵枣粉显著降低荷斯坦公牛呼吸频率(P<0.05),呼吸频率随着发酵枣粉添加量的增加线性降低。当57<89时,M组荷斯坦公牛躺卧率最高,随着发酵枣粉添加量增加,荷斯坦公牛躺卧率呈二次升高变化(P<0.001)。2) C组、L组、M组之间平均日增重无显著差异(P>0.05),但H组平均日增重显著低于其他3组(P<0.05);L组、M组荷斯坦公牛体高增长显著高于C组和H组(P<0.05)。3) M组粗蛋白质表观消化率显著高于其他组(P<0.05),粗脂肪表观消化率随着发酵枣粉添加量增加呈线性增加(P<0.001)。4)试验第30天,H组荷斯坦公牛血清三碘甲腺原氨酸(T3)的含量显著高于C组(P<0.05),血清T3含量随着发酵枣粉添加量增加而呈线性增加(P=0.017)。M组血清总蛋白(TP)含量显著高于对照组(P<0.05),且随着发酵枣粉添加量增加,血清TP含量有二次升高趋势。荷斯坦公牛血清尿素氮(UN)含量随着发酵枣粉添加量增加呈二次下降变化(P=0.010)。试验第90天,M组和H组荷斯坦公牛血清热休克因子-1(HSF-1)含量显著高于对照组(P<0.05)。5)试验第30天,M组血清白细胞介素-1(IL-1)、白细胞介素-6(IL-6)含量显著降低(P<0.05),H组血清免疫球蛋白A (IgA)含量显著高于其他3组(P<0.05);试验第90天,M组和H组血清IL-1含量显著低于对照组(P<0.05);随着发酵枣粉添加量的增加,血清IgA含量呈线性升高(P=0.019)。6)试验第30天,随着发酵枣粉添加量的增加,血清总抗氧化能力(T-AOC)呈线性上升趋势(P=0.137);试验第90天,M组荷斯坦公牛血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化氢酶(GSH-Px)活性、T-AOC显著提高(P<0.05),同时血清丙二醛(MDA)含量显著降低(P<0.05)。综上所述,在本试验条件下,饲粮添加10%发酵枣粉能有效提高高温季节荷斯坦公牛生长性能,降低呼吸频率,提高躺卧率,改善免疫性能,增强奶公牛的抗氧化性能。

本文引用格式

张喆萍 , 李瑞银 , 李树静 , 李建国 , 沈宜钊 , 高艳霞 , 李妍 , 范京惠 , 谢鹏 , 李秋凤 , 曹玉凤 . 饲粮添加发酵枣粉对高温季节荷斯坦公牛生长性能、免疫性能和抗氧化性能的影响[J]. 动物营养学报, 2022 , 34(2) : 1027 -1039 . DOI: 10.3969/j.issn.1006-267x.2022.02.034

Abstract

The objective of this study was to investigate the effects of dietary jujube fermented feed on growth performance, antioxidant performance and immune performance in dairy bulls under hot season. Sixty healthy 4 to 6-month Holstein bulls with similar body weight of (200.00±30.28) kg were randomly into four groups with 15 bulls in each group, the bulls were diets supplemented with 0 (group C group), 5% (group L),10% (group M) or 15% (group H) of jujube fermented feed, respectively. The experimental adaptation period was 7 d and experimental period was 90 d. The results showed as follows:1) when temperature-humidity index (THI)<78, the respiration of rate decreased significantly (P<0.05), and the respiratory frequency decreased linearly with the increase of fermented jujube powder. When 57<89, among all groups, the lying rate of Holstein bulls in group M was the highest, and the lying rate increased twice with the increase of fermented jujube powder (P<0.001). 2) There was no significant in the average daily gain of Holstein bulls among group L, group M, and group H(P>0.05), but the average daily gain in group H was significantly lower than that in the other three groups(P<0.05). The body height of dairy bulls in group L and group M was significantly higher than that in group C and group H (P<0.05). 3) The apparent digestibility of crude protein in group M was significantly higher than that in other groups(P<0.05). The apparent digestibility of crude fat increased linearly with the increase of jujube powder (P<0.001). 4) On the 30th day of the experiment, the content of serum triiodothyronine (T3) in group H was significantly higher than that in group C (P<0.05), and the content of serum T3 increased linearly with the increase of fermented jujube powder (P=0.017). The content of serum total protein (TP) in group M was significantly higher than that in the control group (P<0.05), and showed a secondary increasing trend with the increase of fermented jujube powder. The content of serum urea nitrogen (UN) showed a secondary downward trend with the increase of fermented jujube powder(P=0.010). On the 90th day of the experiment, the content of serum heat shock factor-1 (HSF-1) in the groups M and H was significantly higher than that in the group C (P<0.05). 5) On the 30th day of the experiment, the content of serum interleukin-1 (IL-1) and interleukin-6 (IL-6) decreased significantly in the group M (P<0.05), and the serum immunoglobulin A (IgA) content in the group H was significantly higher than that in the other three groups (P<0.05). On the 90th day, the serum IL-1 content in the groups M and H was significantly lower than that in the group C(P<0.05). With the increase of jujube powder addition, the serum IgA content increased linearly (P=0.019). 6) The total antioxidant capacity (T-AOC) in serum increased linearly with the increase of fermented jujube powder in the 30th day of the experiment (P=0.137); on the 90th day of the experiment, the activities of superoxide dismutase (SOD), glutathione catalase (GSH-Px) and T-AOC in serum were significantly increased (P<0.05), and the content of malonaldehyde (MDA) in serum was significantly decreased (P<0.05). In conclusion, the addition of 10% fermented jujube powder can strengthen the growth performance, reduce respiratory frequency, increase lying rate, improve immune performance, and enhance the antioxidant performance of dairy bull.

参考文献

[1] 董晓丽.益生菌的筛选鉴定及其对断奶仔猪、犊牛生长和消化道微生物的影响[D].博士学位论文.北京:中国农业科学院, 2013. DONG X L.Identification of probiotics and effects of probiotics on weaned piglets, calves and the gastrointestinal microbiate[D].Ph.D.Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2013.(in Chinese)
[2] 张莹.热应激蛋白表达与种公牛精液品质相关性的研究[D].硕士学位论文.长春:吉林大学, 2011. ZHANG Y.The study on correlation analysis between the HSPs expression and semen quality of breeding bull[D].Master's Thesis.Changchun:Jilin University, 2011.(in Chinese)
[3] 郑月.锦江牛与西门塔尔牛耐热性和脂质代谢及肉品质的比较研究[D].博士学位论文.南京:南京农业大学, 2017. ZHENG Y.Comparative study on the heat-tolerance, lipid metabolism and meat quality between Jinjiang and Simmental beef[D].Ph.D.Thesis.Nanjing:Nanjing Agricultural University, 2017.(in Chinese)
[4] 张卫兵, 刁其玉, 赵广城.从营养和饲养角度加强后备牛的培育[J].乳业科学与技术, 2008, 31(1):24-27. ZAHNG W B, DIAO Q Y, ZHAO G C.Improve the dairy replacements through nutrition and feeding management[J].Journal of Dairy Science and Technology, 2008, 31(1):24-27. (in Chinese)
[5] 冯建刚, 张志强, 拓守珍, 等.育成牛的生长发育与营养管理[J].中国奶牛, 2019(11):22-24. FENG J G, ZHANG Z Q, TA S Z, et al.Growth and nutrition management of breeding cattle[J].China Dairy Cattle, 2019(11):22-24.(in Chinese)
[6] 马可为, 赵艳秀, 刘彦慈, 等.枣粉对蛋鸡脂质代谢、抗氧化性能及免疫功能的影响[J].中国饲料, 2017(19):5-8. MA K W, ZHAO Y X, LIU Y C, et al.Effects of jujube powder on lipid metabolism, antioxidant ability and immune function in laying hens[J].China Feed, 2017(19):5-8.(in Chinese)
[7] 牛佳卉, 袁静, 魏然, 等.红枣多糖对小鼠肠道免疫屏障的保护作用及机制研究[J].食品工业科技, 2021, 42(4):295-300, 306. NIU J H, YUAN J, WEI R, et al.Protective effect and mechanism study of jujube polysaccharides on intestinal immune barrier in mice[J].Science and Technology of Food Industry, 2021, 42(4):295-300, 306.(in Chinese)
[8] 陈晓校.枣粉日粮对奶牛瘤胃发酵及产奶性能的影响[D].硕士学位论文.晋中:山西农业大学, 2019. CHEN X J.The effect of wasted Chinese jujube on ruminal fermentation and milk performance in dairy cows[D].Master's Thesis.Jinzhong:Shanxi Agricultural University, 2019.(in Chinese)
[9] 冀建军, 王俊萍, 丁兆忠, 等.生物发酵枣粉在肉牛育肥日粮中的应用研究[J].中国草食动物科学, 2015, 35(6):77-78. JI J J, WANG J P, DING Z Z, et al.Study on the application of bio-fermented jujube powder in fattening diet of beef cattle[J].China Herbivore Science, 2015, 35(6):77-78.(in Chinese)
[10] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社, 2003. ZHANG L Y.Feed analysis and feed quality testing technology[M].2nd ed.Beijing:China Agricultural University Press, 2003.(in Chinese)
[11] 廖晓霞, 叶均安.泌乳奶牛热应激研究进展[J].中国饲料, 2005(19):21-23. LIAO X X, YE J A.Recent advances of heat stress in lactating cow[J].China Feed, 2005(19):21-23.(in Chinese)
[12] 崔施展, 贾东升, 谢晓亮, 等.枣仁颗粒镇静催眠作用及机制研究[J].食品研究与开发, 2017, 38(3):173-176. CUI S Z, JIA D S, XIE X L, et al.Sedative and hypnotic effect of Zizyphi spinosae semen granule and its mechanism[J].Food Research and Development, 2017, 38(3):173-176.(in Chinese)
[13] 李桦, 屈倩, 刘翠, 等.王老吉、香薷散对热应激大鼠HSP70、皮质醇和抗氧化功能的影响[J].畜牧兽医科学(电子版), 2019(17):1-3. LI H, QU Q, LIU C, et al.Effects of Wanglaoji and Xiangqi powder on HSP70, cortisol and antioxidant function in heat stress[J].Graziery Veterinary Sciences(Electronic Version), 2019(17):1-3.(in Chinese)
[14] 吝常华, 王冰, 蔡辉益.肉鸡发酵饲料生产技术的研究进展[J].中国畜牧杂志, 2017, 53(5):4-10. LIN C H, WANG B, CAI H Y.Research progress of fermented feed production technology of broiler[J].Chinese Journal of Animal Science, 2017, 53(5):4-10.(in Chinese)
[15] 冯平, 孙旺斌, 张骞, 等.枣粉对陕北白绒山羊肉抗氧化性能的影响[J].农业工程学报, 2020, 36(11):310-317. FENG P, SUN W B, ZHANG Q, et al.Effects of jujube powder on the antioxidant capability of North Shaanxi white cashmere goat meat[J].Transactions of the Chinese Society of Agricultural Engineering, 2020, 36(11):310-317.(in Chinese)
[16] 崔耀明, 管军军, 王金荣, 等.益生乳酸菌对肉鸡骨骼发育的影响及作用机制研究进展[J].动物营养学报, 2021, 33(1):13-19. CUI Y M, GUAN J J, WANG J R, et al.Research progress on effects and mechanism of probiotic lactic acid bacteria on broilers' bone development[J].Chinese Journal of Animal Nutrition, 2021, 33(1):13-19.(in Chinese)
[17] 张萌萌, 张秀珍, 邓伟民, 等.骨代谢生化指标临床应用专家共识(2019)[J].中国骨质疏松杂志, 2019, 25(10):1357-1372. ZHANG M M, ZHANG X Z, DENG W M, et al.Expert consensus on clinical application of biochemical indicators of bone metabolism (2019)[J].Chinese Journal of Osteoporosis, 2019, 25(10):1357-1372.(in Chinese)
[18] 吴晓龙, 谢艳, 邹吉锋, 等.酸枣仁提取物通过调节Gi/o蛋白表达抑制cAMP-PKA通路促进小鼠骨骼增长的研究[J].黑龙江医药科学, 2020, 43(3):146-148. WU X L, XIE Y, ZOU J F, et al.Study on the effect of semen Ziziphi spinosae extract on bone growth in mice by inhibiting cAMP-PKA pathway by regulating the expression of Gi/o protein[J].Heilongjiang Medicine and Pharmacy, 2020, 43(3):146-148.(in Chinese)
[19] 吴晓龙, 谢艳, 刘晓红, 等.酸枣仁提取物配柴胡提取物组方促进动物身高增高及机制研究[J].药品评价, 2019, 16(16):1-3. WU X L, XIE Y, LIU X H, et al.Effects and mechanism of semen Ziziphi spinosae and Bupleurum extract compound on promoting height of animals[J].Drug Evaluation, 2019, 16(16):1-3.(in Chinese)
[20] AHMED L A, SCHIRMER H, BERNTSEN G K, et al.Self-reported diseases and the risk of non-vertebral fractures:the Tromsø study[J].Osteoporosis International, 2006, 17(1):46-53.  
[21] 任大勇.益生乳酸杆菌的黏附及免疫调节作用研究[D].博士学位论文.长春:吉林大学, 2013. REN D Y.Research on adhesion and immunoregulation of probiotic Lactobacillus strains[D]. Ph.D.Thesis.Changchun:Jilin University, 2013.(in Chinese)
[22] LIU F, YIN J, DU M, et al.Heat-stress-induced damage to porcine small intestinal epithelium associated with downregulation of epithelial growth factor signaling[J].Journal of Animal Science, 2009, 87(6):1941-1949.  
[23] BAO E D, SULTAN K R, NOWAK B, et al.Expression and distribution of heat shock proteins in the heart of transported pigs[J].Cell Stress & Chaperones, 2008, 13(4):459-466.  
[24] 马燕芬, 杜瑞平, 高民.热应激对奶山羊生产性能及瘤胃上皮细胞形态结构的影响[J].中国农业科学, 2013, 46(21):4486-4495. MA Y F, DU R P, GAO M.Effect of heat stress on dairy goat performance and rumen epithelial cell morphology[J].Scientia Agricultura Sinica, 2013, 46(21):4486-4495.(in Chinese)
[25] BERNABUCCI U, LACETERA N, DANIELI P P, et al.Influence of different periods of exposure to hot environment on rumen function and diet digestibility in sheep[J].International Journal of Biometeorology, 2009, 53(5):387-395.  
[26] 姜义宝, 王美静, 王成章, 等.苜蓿和红三叶黄酮类物质对肉羊生长性能、血液生化指标和激素的影响[C]//第六届(2015)中国苜蓿发展大会暨国际苜蓿会议论文集, 蚌埠:中国畜牧业协会, 2015:40-46. JIANG Y B, WANG M J, WANG C Z, et al.Effect of flavonoids which come from alfalfa and trifolium pretense on growth performance, blood indices and hormone of mutton sheep[C]//Compilation of Papers of the 6th (2015) China Alfalfa Development Conference and International Alfalfa Conference, Bengbu:China Animal Husbandry Association, 2015:40-46.(in Chinese)
[27] 马国强.黑沙蒿及其多糖对绒山羊瘤胃发酵参数、营养物质消化、血液抗氧化与免疫指标的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学, 2019. MA G Q.Effects of Artemisia ordosica and its polysaccharides on ruminal fermentation, nutrient digestibility, serum antioxidant and immune parameters of cashmere goats[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University, 2019.(in Chinese)
[28] 符运斌.金银花绿原酸的提取及其对热应激肉牛抗氧化功能的影响研究[D].硕士学位论文.南昌:江西农业大学, 2016. FU Y B.Extraction of chlorogenic acid from honeysukle and influence on antioxidant functions in beef cattle under heat stress[D].Master's Thesis.Nanchang:Jiangxi Agricultural University, 2016.(in Chinese)
[29] STANLEY C C, WILLIAMS C C, JENNY B F, et al.Effects of feeding milk replacer once versus twice daily on glucose metabolism in Holstein and Jersey calves[J].Journal of Dairy Science, 2002, 85(9):2335-2343.  
[30] ZACHUT M, KRA G, LIVSHITZ L, et al.Seasonal heat stress affects adipose tissue proteome toward enrichment of the Nrf2-mediated oxidative stress response in late-pregnant dairy cows[J].Journal of Proteomics, 2017, 158:52-61.
[31] 刘宇宸, 王向明, 万平民, 等.热应激引起动物炎症反应的研究进展[J].中国兽医杂志, 2019, 55(3):57-68. LIU Y C, WANG X M, WAN P M, et.Mechanism of oxidative stress in body under heat stress[J].Chinese Journal of Veterinary Medicine, 2019, 55(3):57-68.(in Chinese)
[32] 张玉侠.热应激对奶牛维持行为、免疫功能和外周血microRNAs影响的研究[D].硕士学位论文.武汉:华中农业大学, 2020. ZHANG Y X.Study on the influence of heat stress on the maintenance bahavior, immune function and blood microRNAs of cows[D].Master's Thesis.Wuhan:Huazhong Agricultural University, 2020.(in Chinese)
[33] JOMOVÁ K, HUDECOVA L, LAURO P, et al.A switch between antioxidant and prooxidant properties of the phenolic compounds myricetin, morin, 3', 4'-dihydroxyflavone, taxifolin and 4-hydroxy-coumarin in the presence of copper(Ⅱ) ions:a spectroscopic, absorption titration and DNA damage study[J].Molecules, 2019, 24(23):4335.
[34] BANJARE J, SALUNKE M, INDAPURKAR K, et al.Estimation of serum malondialdehyde as a marker of lipid peroxidation in medical students undergoing examination-induced psychological stress[J].Journal of the Scientific Society, 2017, 44(3):137-139.  
[35] 谢虹.黄酮类化合物的RAF抗氧化机制[D].硕士学位论文.广州:广州中医药大学, 2019. XIE H.The radical adduct formation mechanism of flavonoids[D].Master's Thesis.Guangzhou:Guangzhou University of Chinese Medicine, 2019.(in Chinese)
[36] LI J, JIANG N, ZHANG A, et al.Expression of IL-1 family in immune system[J].Probe-Animal Science, 2020, 2(1):1311.
[37] 黄晶, 张冰, 林志健.中药对白介素类细胞因子的影响及其在防治COVID-19炎症风暴中的思考[J].中药药理与临床, 2020, 36(2):23-28. HUANG J, ZHANG B, LIN Z J.Intervention effect of Chinese medicine on interleukin cytokines and thinking on its prevention and treatment for inflammatory storm of COVID-19[J].Pharmacology and Clinics of Chinese Materia Medica, 2020, 36(2):23-28.(in Chinese)
[38] 温超, 王恬, 周岩民.不同酶制剂对蛋鸡生产性能、鸡蛋品质和血清生化指标的影响[C]//第六次全国饲料营养学术研讨会论文集, 杨凌:中国畜牧兽医学会, 2010:1. WEN C, WANG T, ZHOU Y.Effects of different enzyme preparations on production performance, egg quality and serum biochemical indexes of laying hens[C]//Proceedings of the Sixth National Symposium on Feed Nutrition, Yangling:Chinese Society of Animal Husbandry and Veterinary Medicine, 2010:1.(in Chinese)
[39] ZHANG L Y, LI N, CAICEDO R, et al.Alive and dead Lactobacillus rhamnosus GG decrease tumor necrosis factor-alpha-induced interleukin-8 production in Caco-2 cells[J].Journal of Nutrition, 2005, 135(7):1752-1756.  
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