研究论文 RESEARCH PAPER

饲料中添加缩合单宁对凡纳滨对虾生长性能、体成分、血清生化及抗氧化指标的影响

  • 彭凯 ,
  • 陈冰 ,
  • 莫文艳 ,
  • 吴汉 ,
  • 黄文
展开
  • 广东省农业科学院动物科学研究所, 广东省畜禽育种与营养研究重点实验室, 农业农村部 华南动物营养与饲料重点实验室, 广州 510640
彭凯(1987-),男,河南信阳人,副研究员,博士,主要从事水产动物营养与饲料研究。E-mail:pengkai1016@126.com

收稿日期: 2021-07-01

  网络出版日期: 2022-02-15

基金资助

国家自然科学基金(31902388);广东省自然科学基金面上项目(2018A0303130301,2021A1515010850);广州市基础与应用基础研究项目(202002030378);广东省农业科学院院长基金面上项目(201909);科技创新战略专项资金(高水平农科院建设)(R2018QD-075);2021乡村振兴战略专项资金(农业科技能力提升)(TS-1-6-17)

Effects of Dietary Condensed Tannins on Growth Performance, Body Composition, Serum Biochemical and Antioxidant Indexes of Litopenaeus vannamei

  • PENG Kai ,
  • CHEN Bing ,
  • MO Wenyan ,
  • WU Han ,
  • HUANG Wen
Expand
  • Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2021-07-01

  Online published: 2022-02-15

摘要

本试验旨在研究饲料中添加缩合单宁对凡纳滨对虾生长性能、体成分、血清生化及抗氧化指标的影响。选择初体重约为1.38 g的凡纳滨对虾600尾,随机分为5组,每组3个重复,每个重复40尾。5组对虾投喂缩合单宁添加量分别为0(CT0组,作为对照组)、0.5(CT0.5组)、1.0(CT1组)、2.0(CT2组)、4.0 g/kg DM (CT4组)的5种等氮等脂试验饲料,试验期56 d。结果表明:随着饲料中缩合单宁添加量的增加,凡纳滨对虾的终末体重、增重率和特定生长率呈线性增加的趋势(0.05<P<0.10),饲料系数呈线性降低的趋势(0.05<P<0.10);各组间凡纳滨对虾的成活率、摄食量、肥满度和肝体比无显著差异(P>0.05)。饲料中添加缩合单宁对凡纳滨对虾体成分、肌肉氨基酸含量和血清生化指标均未产生显著影响(P>0.05)。与CT0组相比,饲料中添加1.0~2.0 g/kg DM缩合单宁显著增加了血清过氧化氢酶和超氧化物歧化酶活性(P<0.05),显著降低了血清丙二醛含量(P<0.05)。CT4组凡纳滨对虾血清过氧化氢酶和超氧化物歧化酶活性显著低于CT0组(P<0.05)。综上所述,饲料中添加适量缩合单宁提高了凡纳滨对虾的生长性能和抗氧化能力,但不影响体成分、肌肉氨基酸含量和血清生化指标。以血清超氧化物歧化酶活性为评价指标,凡纳滨对虾饲料中缩合单宁的最适添加量为1.94 g/kg DM。

本文引用格式

彭凯 , 陈冰 , 莫文艳 , 吴汉 , 黄文 . 饲料中添加缩合单宁对凡纳滨对虾生长性能、体成分、血清生化及抗氧化指标的影响[J]. 动物营养学报, 2022 , 34(2) : 1165 -1174 . DOI: 10.3969/j.issn.1006-267x.2022.02.048

Abstract

This study was conducted to investigate the effects of dietary condensed tannins on growth performance, body composition, serum biochemical and antioxidant indexes of Litopenaeus vannamei. A total of 600 Litopenaeus vannamei with an initial body weight of approximately 1.38 g were randomly divided into 5 groups with 3 replicates per group and 40 shrimps per replicate. Shrimps in the 5 groups were fed 5 isonitrogenous and isolipidic experimental diets, which supplemented with 0 (CT0 group, as control group), 0.5 (CT0.5 group), 1.0 (CT1 group), 2.0 (CT2 group) and 4.0 g/kg DM (CT4 group) of condensed tannins based on a basal diet, respectively. The feeding trial lasted for 65 days. The results showed that with the increase of dietary condensed tannins supplemental level, the final body weight, weight gain rate and specific growth rate of shrimps increased with a linear effect (0.05<P<0.10), and the feed coefficient decreased with a linear effect (0.05<P<0.10), and there were no significant differences in survival rate, feed intake, condition factor and hepatosomatic index among all groups (P>0.05). Dietary condensed tannins did not affect the body composition, amino acid contents in muscle and serum biochemical indexes (P>0.05). Compared with CT0 group, diet supplemented with 1.0 to 2.0 g/kg DM condensed tannins significantly increased the serum catalase and superoxide dismutase activities (P<0.05), and significantly decreased serum malondialdehyde content (P<0.05). The serum catalase and superoxide dismutase activities in the CT4 group were significantly lower than those in the CT0 group (P<0.05). In conclusion, dietary appropriate amount of condensed tannins can improve the growth performance and antioxidant capacity of Litopenaeus vannamei, but do not affect body composition, muscle amino acid contents and serum biochemical indexes. Using serum superoxide dismutase activity as evaluation index, the optimal supplemental level of dietary condensed tannins is 1.94 g/kg DM.

参考文献

[1] 彭凯, 王玉玺, 王国霞, 等.缩合单宁的生物功能及其在动物生产中的应用[J].动物营养学报, 2020, 32(8):3451-3460. PENG K, WANG Y X, WANG G X, et al.Biological function of condensed tannins and their application in animal production[J].Chinese Journal of Animal Nutrition, 2020, 32(8):3451-3460.(in Chinese)
[2] HUANG Q Q, LIU X L, ZHAO G Q, et al.Potential and challenges of tannins as an alternative to in-feed antibiotics for farm animal production[J].Animal Nutrition, 2018, 4(2):137-150.  
[3] BECKER K, MAKKAR H P S.Effects of dietary tannic acid and quebracho tannin on growth performance and metabolic rates of common carp (Cyprinus carpio L.)[J].Aquaculture, 1999, 175(3/4):327-335.
[4] OMNES M H, LE GOASDUFF J, LE DELLIOU H, et al.Effects of dietary tannin on growth, feed utilization and digestibility, and carcass composition in juvenile European seabass (Dicentrarchus labrax L.)[J].Aquaculture Reports, 2017, 6:21-27.
[5] LI M, FENG L, JIANG W D, et al.Condensed tannins decreased the growth performance and impaired intestinal immune function in on-growing grass carp (Ctenopharyngodon idella)[J].The British Journal of Nutrition, 2020, 123(7):737-755.  
[6] 卢俊姣.饲料中添加原花青素对吉富罗非鱼生长及氧化应激的影响[D].硕士学位论文.厦门:集美大学, 2014. LU J J.The effects of dietary oligomeric proanthocyanidins supplementation on the growth and oxidative stress of juvenile GIFT tilapia (Oreochromis niloticus)[D].Master's Thesis.Xiamen:Jimei University, 2014.(in Chinese)
[7] PENG K, WANG G X, WANG Y X, et al.Condensed tannins enhanced antioxidant capacity and hypoxic stress survivability but not growth performance and fatty acid profile of juvenile Japanese seabass (Lateolabrax japonicus)[J].Animal Feed Science and Technology, 2020, 269:114671.
[8] PENG K, ZHOU Y H, WANG Y X, et al.Inclusion of condensed tannins in Lateolabrax japonicus diets:effects on growth, nutrient digestibility, antioxidant and immune capacity and copper sulphate stress resistance[J].Aquaculture Reports, 2020, 18:100525.
[9] BUYUKCAPAR H M, ATALAY A I, KAMALAK A.Growth performance of Nile tilapia (Oreochromis niloticus) fed with diets containing different levels of hydrolysable and condensed tannin[J].Journal of Agricultural Science and Technology, 2011, 13:1045-1051.
[10] 朱旭枫, 黄洋, 黄俊杭, 等.水解单宁对珍珠龙胆石斑鱼生长性能、抗氧化能力、肠道组织结构与菌群多样性的影响[J].动物营养学报, 2021, 33(2):1020-1035. ZHU X F, HUANG Y, HUANG J H, et al.Effects of hydrolyzable tannins on growth performance, antioxidant capacity, intestinal morphology and bacterial diversity of pearl gentian grouper (Epinephelus lanceolatus ♂×Epinephelus fuscoguttatus ♀)[J].Chinese Journal of Animal Nutrition, 2021, 33(2):1020-1035.(in Chinese)
[11] 郭慧, 朱旭枫, 陈锦霖, 等.水解单宁对凡纳滨对虾生长性能和肠道微生物的影响[J].中国水产科学, 2019, 26(5):883-892. GUO H, ZHU X F, CHEN J L, et al.Effects of hydrolyzable tannins on growth performance and intestinal microflora in Litopenaeus vannamei[J].Journal of Fishery Sciences of China, 2019, 26(5):883-892.(in Chinese)
[12] 朱旭枫, 邓秋霞, 郭慧, 等.水解单宁对副溶血弧菌感染凡纳滨对虾血液及血细胞免疫指标的影响[J].广东海洋大学学报, 2021, 41(3):12-19. ZHU X F, DENG Q X, GUO H, et al.Effect of hydrolyzable tannins on hemolymph and cellular immunological responses of Litopenaeus vannamei challenged by vibrio parahaemolyticus[J].Journal of Guangdong Ocean University, 2021, 41(3):12-19.(in Chinese)
[13] 彭凯.贮藏方式对饲用紫色达利菊缩合单宁的化学结构及生物活性的影响研究[D].博士学位论文.北京:中国农业大学, 2017. PENG K.Effects of conservation methods on chemical structure and biological activity of condensed tannins in purple prairie clover[D].Ph.D.Thesis.Beijing:China Agricultural University, 2017.(in Chinese)
[14] TERRILL T H, ROWAN A M, DOUGLAS G B, et al.Determination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains[J].Journal of the Science of Food and Agriculture, 1992, 58(3):321-329.  
[15] PENG K, JIN L, NIU Y D, et al.Condensed tannins affect bacterial and fungal microbiomes and mycotoxin production during ensiling and upon aerobic exposure[J].Applied and Environmental Microbiology, 2018, 84(5):e02274-17.
[16] BIAGIA G, CIPOLLINI I, PAULICKS B R, et al.Effect of tannins on growth performance and intestinal ecosystem in weaned piglets[J].Archives of Animal Nutrition, 2010, 64(2):121-135.  
[17] PENG K, ZHAO H X, WANG G X, et al.Effect of condensed tannins on growth performance, intestinal immune capacity and bacterial microbiomes of Lateolabrax japonicus[J].Aquaculture Research, 2021, 52(11):5321-5331.  
[18] PRZYWITOWSKI M, MIKULSKI D, ZDUNCZYK Z, et al.The effect of dietary high-tannin and low-tannin faba bean (Vicia faba L.) on the growth performance, carcass traits and breast meat characteristics of finisher turkeys[J].Animal Feed Science and Technology, 2016, 221(Part A):124-136.
[19] 姚静婷, 孔纯, 华雪铭, 等.水解单宁对暗纹东方鲀摄食偏好、消化代谢和抗氧化能力的效应[J].水产学报, 2019, 43(6):1449-1462. YAO J T, KONG C, HUA X M, et al.Effects of supplemental hydrolysable tannin on feeding preference, nutrition digestion and antioxidant ability of obscure puffer (Takifugu fasciatus)[J].Journal of Fisheries of China, 2019, 43(6):1449-1462.(in Chinese)
[20] 王爱民, 韩光明, 封功能, 等.饲料脂肪水平对吉富罗非鱼生产性能、营养物质消化及血液生化指标的影响[J].水生生物学报, 2011, 35(1):80-87. WANG A M, HAN G M, FENG G N, et al.Effects of dietary lipid levels on growth performance, nutrient digestibility and blood biochemical indices of gift tilapia (Oreochromis niloticus)[J].Acta Hydrobiologica Sinica, 2011, 35(1):80-87.(in Chinese)
[21] PENG K, SHIRLEY D C, XU Z J, et al.Effect of purple prairie clover (Dalea purpurea Vent.) hay and its condensed tannins on growth performance, wool growth, nutrient digestibility, blood metabolites and ruminal fermentation in lambs fed total mixed rations[J].Animal Feed Science and Technology, 2016, 222:100-110.
[22] MENG Q G, CHEN J, XU C C, et al.The characterization, expression and activity analysis of superoxide dismutases (SODs) from Procambarus clarkii[J].Aquaculture, 2013, 406/407:131-140.
[23] DING Z L, ZHANG Y X, YE J Y, et al.An evaluation of replacing fish meal with fermented soybean meal in the diet of Macrobrachium nipponense:growth, nonspecific immunity, and resistance to Aeromonas hydrophila[J].Fish & Shellfish Immunology, 2015, 44(1):295-301.  
[24] BARREIRA J C M, FERREIRA I C F R, OLIVEIRA M B P P, et al.Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit[J].Food Chemistry, 2008, 107(3):1106-1113.  
[25] KOLECKAR V, KUBIKOVA K, REHAKOVA Z, et al.Condensed and hydrolysable tannins as antioxidants influencing the health[J].Mini Reviews in Medicinal Chemistry, 2008, 8(5):436-447.  
文章导航

/