动物营养学报 >

2022 , Vol. 34 >Issue 2: 1230 - 1239

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.02.054

研究论文 RESEARCH PAPER

白藜芦醇对氧化应激仔猪肝脏抗氧化酶、细胞因子mRNA表达及核因子E2相关因子2相关因子信号通路的影响

  • 姬孟瑶 ,
  • 荀文娟 ,
  • 侯冠彧 ,
  • 施力光 ,
  • 侯晓晓 ,
  • 杨文
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  • 1. 海南大学动物科技学院, 海口 570228;
    2. 中国热带农业科学院热带作物品种资源研究所, 儋州 571737
姬孟瑶(1997-),女,新疆昌吉人,硕士研究生,从事猪营养研究。E-mail:282010944@qq.com

收稿日期: 2021-07-12

  网络出版日期: 2022-02-15

基金资助

海南大学科研启动费[KYQD（ZR）20037]；海南省科协青年科技英才学术创新计划项目（QCXM201907）；国家自然科学基金地区基金项目（32060757）；中国热带农业科学院基本科研业务费专项资金（1630032017035）

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Effects of Resveratrol on Liver Antioxidant Enzyme, Cytokine mRNA Expression and Nuclear Factor Erythroid 2 Related Factor 2 Signaling Pathway in Oxidative Stress Piglets

  • JI Mengyao ,
  • XUN Wenjuan ,
  • HOU Guanyu ,
  • SHI Liguang ,
  • HOU Xiaoxiao ,
  • YANG Wen
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  • 1. College of Animal Science and Technology, Hainan University, Haikou 570228, China;
    2. Institute of Tropical Crop Variety Resources, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Received date: 2021-07-12

  Online published: 2022-02-15

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摘要

本试验旨在研究白藜芦醇（RES）对敌草快（diquat）诱导的氧化应激仔猪肝脏抗氧化、抗炎性能的影响。选用健康断奶仔猪30头，随机分为5组，对照组（CON组）和diquat组（DIQ组）饲喂基础饲粮，试验组（RES-10组、RES-30组、RES-90组）饲喂在基础饲粮中分别添加10、30、90 mg/kg RES的试验饲粮。于试验第15天清晨给DIQ组、RES-10组、RES-30组、RES-90组仔猪按照10 mg/kg BW剂量腹腔注射diquat建立氧化应激模型，CON组注射相同剂量灭菌生理盐水。试验期21 d。结果表明：与CON组相比，DIQ组肝脏超氧化物歧化酶1（SOD1）、超氧化物歧化酶2（SOD2）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶-1（GPX-1）的mRNA相对表达量均显著降低（P<0.05）；肝脏白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的mRNA相对表达量显著升高（P<0.05），而白细胞介素-10（IL-10）的mRNA相对表达量显著降低（P<0.05）；肝脏Kelch样ECH关联蛋白1（Keap1）的mRNA相对表达量显著升高（P<0.05），核因子E2相关因子2（Nrf2）和醌氧化还原酶1（NQO1）的mRNA相对表达量显著降低（P<0.05）；肝脏Keap1和Nrf2蛋白表达量分别上升和下降，NQO1的蛋白表达量则呈现显著下降（P<0.05）。与DIQ组相比，RES-10组、RES-30组、RES-90组肝脏SOD1、SOD2、CAT、GPX-1的mRNA相对表达量均显著上调（P<0.05）；RES-30组、RES-90组肝脏TNF-α、IL-1β、IL-6的mRNA相对表达量均显著下调（P<0.05），同时肝脏IL-10的mRNA相对表达量显著上调（P<0.05），且RES-90组效果最显著；RES-10组、RES-30组、RES-90组肝脏Keap1的mRNA相对表达量均显著下调（P<0.05），同时肝脏Nrf2、NQO1的mRNA相对表达量显著上调（P<0.05），且RES-90组效果最优；肝脏Keap1蛋白表达量在RES-90组显著下降（P<0.05），Nrf2蛋白表达量在RES-90组有上升的趋势（P=0.057）。综上所述，RES可以提高氧化应激仔猪肝脏抗氧化、抗炎性能，降低肝脏氧化应激程度，其发挥抗氧化机制可能与Nrf2信号通路有关。

关键词： 白藜芦醇; 仔猪; 氧化应激; 肝脏; 抗氧化; 抗炎

本文引用格式

姬孟瑶 , 荀文娟 , 侯冠彧 , 施力光 , 侯晓晓 , 杨文 . 白藜芦醇对氧化应激仔猪肝脏抗氧化酶、细胞因子mRNA表达及核因子E2相关因子2相关因子信号通路的影响[J]. 动物营养学报, 2022 , 34(2) : 1230 -1239 . DOI: 10.3969/j.issn.1006-267x.2022.02.054

Abstract

The purpose of this experiment was to study resveratrol (Res) on the liver anti-oxidative and anti-inflammatory capacity in diquat challenged oxidative stress piglets. Thirty weaned piglets with good health were selected and randomly divided into 5 groups, the control group (CON group) and diquat group (DIQ group) was fed a basal diet, RES of 10, 30 and 90 mg/kg were added to the basal diet in the experimental groups (RES-10 group, RES-30 group, RES-90 group), respectively. In the early morning of the 15th day of the experiment, the piglets in the DIQ group, RES-10 group, RES-30 group and RES-90 group were intraperitoneally injected with diquat at a dose of 10 mg/kg BW to establish an oxidative stress model, and the control group injected the same dose of sterilized saline as a reference. The trial period was 21 days. The results showed as follows:compared with the CON group, the liver superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), catalase (CAT) and glutathione peroxidase-1 (GPX-1) mRNA relative expression levels were all significantly decreased in the DIQ group (P<0.05); the liver interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) mRNA relative expression levels were significantly increased (P<0.05), while the liver interleukin-10 (IL-10) mRNA relative expression level was significantly reduced (P<0.05); the liver Kelch-like ECH-associated protein 1 (Keap1) mRNA relative expression level was significantly increased (P<0.05), while the liver nuclear factor erythroid 2 related factor 2 (Nrf2) and NADPH quinone oxidoreductase 1 (NQO1) mRNA relative expression levels were significantly reduced (P<0.05); the liver Keap1 and Nrf2 protein expression levels were increased and decreased, respectively, and the NQO1 protein expression level was reduced significantly (P<0.05). Compared with the DIQ group, SOD1, SOD2, CAT and GPX-1 mRNA relative expression level was significantly increased in the RES-10 group, RES-30 group and RES-90 group (P<0.05); in the RES-30 group and RES-90 group, liver TNF-α, IL-1β and IL-6 mRNA relative expression levels were significantly reduced (P<0.05), meanwhile, IL-10 mRNA relative expression level was significantly increased (P<0.05), among which the RES-90 group had the most significant effect; liver Keap1 mRNA expression level was significantly dropped in the RES-10 group, RES-30 group and RES-90 group (P<0.05), while the Nrf2 and NQO1 mRNA relative expression levels were significantly increased (P<0.05), among which the RES-90 group had the best effect; the liver Keap1 protein relative expression level was significantly decreased in the RES-90 group (P<0.05), and the Nrf2 protein expression level was tend to increase in RES-90 group (P=0.057). It is concluded that RES increase the anti-oxidative and anti-inflammatory capacity in the liver of oxidative stress piglets, and reduce the level of liver oxidative stress, its anti-oxidative mechanism may be related to Nrf2 signaling pathway.

Key words： resveratrol; piglets; oxidative stress; liver; anti-oxidation; anti-inflammation

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