动物营养学报 >

2022 , Vol. 34 >Issue 2: 1260 - 1267

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.02.057

研究论文 RESEARCH PAPER

鸡血管生成素样蛋白4重组蛋白对肉鸡肝脏和胸肌脂肪代谢的影响

  • 侯彦茹 ,
  • 赵旭 ,
  • 黄华山 ,
  • 张金红 ,
  • 井长伟
展开
  • 1. 临沂大学农林科学学院, 临沂 276000;
    2. 山东隆大生物工程有限公司, 临沂 276400;
    3. 山东亚太中慧集团有限公司, 潍坊 262400
侯彦茹(2000-),女,山东菏泽人,本科生,动物科学专业。E-mail:1021647580@qq.com

收稿日期: 2021-07-30

  网络出版日期: 2022-02-15

基金资助

临沂大学高层次人才科研启动项目（LYDX2019BS036）；临沂大学2020年大学生创新创业训练计划项目（X202010452116）

收起

Effects of Chicken Angiopoietin-Like Protein 4 Recombinant Protein on Fat Metabolism in Liver and Breast Muscle of Broilers

  • HOU Yanru ,
  • ZHAO Xu ,
  • HUANG Huashan ,
  • ZHANG Jinhong ,
  • JING Changwei
Expand
  • 1. College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China;
    2. Shandong Longda Bio-Products Co., Ltd., Linyi 276400, China;
    3. Shandong Yatai Zhonghui Group Co., Ltd., Weifang 262400, China

Received date: 2021-07-30

  Online published: 2022-02-15

Fold

摘要

本试验旨在探究鸡血管生成素样蛋白4（ANGPTL4）重组蛋白对肉鸡肝脏和胸肌脂肪代谢的影响。分体内动物试验和体外细胞试验2部分进行。体内动物试验选用35日龄健康禁食状态下爱拔益加肉公鸡36只，随机分为6组，每组6个重复，每个重复1只鸡。对照组翅静脉注射灭菌的生理盐水，试验组分别翅静脉注射20、100、500、2 500、12 500 ng/kg BW鸡ANGPTL4重组蛋白，注射剂量均为550 μL，试验期30 min。体外细胞试验设3个组，分别是生理盐水组、组氨酸-小分子泛素样修饰蛋白（His-SUMO）标签组和鸡ANGPTL4重组蛋白组，分别在细胞培养基中添加灭菌的生理盐水、His-SUMO标签蛋白（其含量与鸡ANGPTL4重组蛋白组中标签蛋白含量一致）和鸡ANGPTL4重组蛋白（250 pg/mL），添加剂量均为5 μL，5% CO2、37℃孵育24 h。结果发现：1）与对照组相比，20、100、500、2 500 ng/kg BW鸡ANGPTL4重组蛋白组的肝脏脂肪酸合成酶（FAS） mRNA相对表达量和500 ng/kg BW鸡ANGPTL4重组蛋白组的肝脏FAS活性均显著提高（P<0.05）。随着鸡ANGPTL4重组蛋白注射剂量增加，肝脏FAS mRNA相对表达量和活性均呈现一次线性和二次曲线增加的效应（P<0.05）。2）与对照组相比，100、500、2 500 ng/kg BW鸡ANGPTL4重组蛋白组的肝脏苹果酸酶（ME） mRNA相对表达量和12 500 ng/kg BW鸡ANGPTL4重组蛋白组的肝脏乙酰辅酶A羧化酶（ACC） mRNA相对表达量均显著降低（P<0.05）。随着鸡ANGPTL4重组蛋白注射剂量增加，肝脏ACC mRNA相对表达量呈现一次线性和二次曲线降低的效应（P<0.05）。不同注射剂量的鸡ANGPTL4重组蛋白对肉鸡肝脏ME和ACC活性均无显著影响（P>0.05）。3）与对照组相比，500 ng/kg BW鸡ANGPTL4重组蛋白组的胸肌脂蛋白脂酶（LPL）活性显著增加（P<0.05）。4）鸡ANGPTL4重组蛋白组的成肌细胞甘油三酯（TG）含量显著高于生理盐水组和His-SUMO标签组（P<0.05）。综上所述，鸡ANGPTL4重组蛋白具有调控肉鸡肝脏脂肪合成相关基因mRNA表达和酶活性以及促进肉鸡胸肌脂肪沉积的作用，以500 ng/kg BW注射剂量效果较好。

关键词： 鸡ANGPTL4重组蛋白; 肉鸡; 肝脏; 胸肌; 脂肪代谢

本文引用格式

侯彦茹 , 赵旭 , 黄华山 , 张金红 , 井长伟 . 鸡血管生成素样蛋白4重组蛋白对肉鸡肝脏和胸肌脂肪代谢的影响[J]. 动物营养学报, 2022 , 34(2) : 1260 -1267 . DOI: 10.3969/j.issn.1006-267x.2022.02.057

Abstract

This experiment aimed to explore the effects of chicken angiopoietin-like protein 4 (ANGPTL4) recombinant protein on fat metabolism in liver and breast muscle of broilers. It was divided into 2 parts:animal experiment in vivo and cell experiment in vitro. In the animal experiment in vivo, thirty-six healthy 35-day-old Arbor Acres broilers under fasting condition were randomly divided into 6 groups with 6 replicates per group and 1 broiler per replicate. Broilers in the control group were intravenously injected with sterile normal saline, and others in experimental groups were intravenously injected 20, 100, 500, 2 500 and 12 500 ng/kg BW chicken ANGPTL4 recombinant protein, respectively. The injection dose was 550 μL, and the experiment period was 30 minutes. Three groups were established for the cell experiment in vitro, including the normal saline group, histidine-small ubiquitin-like modifier (His-SUMO) tag group and chicken ANGPTL4 recombinant protein group, which supplemented 5 μL sterile normal saline, His-SUMO tag protein (its content is consistent with that of chicken ANGPTL4 recombinant protein group) and chicken ANGPTL4 recombinant protein (250 pg/mL), respectively, with 5% CO2 and 37℃ incubated 24 h. The results showed as follows:1) compared with the control group, the liver fatty acid synthase (FAS) mRNA expression level of 20, 100, 500 and 2 500 ng/kg BW chicken ANGPTL4 recombinant protein groups and liver FAS activity of 500 ng/kg BW chicken ANGPTL4 recombinant protein group were significantly increased (P<0.05). With the chicken ANGPTL4 recombinant protein injection dose increased, the liver FAS mRNA expression level and activity showed linear and quadratic increase effects (P<0.05). 2) Compared with the control group, the liver malic enzyme (ME) mRNA expression level of 100, 500 and 2 500 ng/kg BW chicken ANGPTL4 recombinant protein groups and liver acetyl-CoA carboxylase (ACC) mRNA expression level of 12 500 ng/kg BW chicken ANGPTL4 recombinant protein group were significantly decreased (P<0.05). With the chicken ANGPTL4 recombinant protein injection dose increased, the liver ACC mRNA expression level and activity showed linear and quadratic decrease effects (P<0.05). Different injection dose of chicken ANGPTL4 recombinant protein had no significant effects on activities of ME and ACC in liver of broilers (P>0.05). 3) Compared with the control group, the breast muscle lipoprotein lipase (LPL) activity of 500 ng/kg BW chicken ANGPTL4 recombinant protein group was significantly increased (P<0.05). 4) The myoblasts triglyceride (TG) content of chicken ANGPTL4 recombinant protein group was significantly higher than that of normal saline group and His-SUMO tag group (P<0.05). In summary, chicken ANGPTL4 recombinant protein can regulate the mRNA expression and activity of fat synthesis related genes and enzyme in liver of broilers and promote fat deposition in breast muscle of broilers, and the 500 ng/kg BW injection dose has the better effect.

Key words： chicken ANGPTL4 recombinant protein; broilers; liver; breast muscle; fat metabolism

参考文献

[1] 杨烨.优质鸡肌内脂肪代谢调控及其与肉质性状关系的研究[D].博士学位论文.北京:中国农业科学院, 2005. YANG Y.Studies on the metabolism of intramuscular fat in quality chicken and its relationships with meat quality traits[D].Ph.D.Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2005.(in Chinese)
[2] 司彦培, 刘国华, 刘志云.益生素的作用机制及其在家禽饲料中的应用进展[J].中国家禽, 2016, 38(5):42-46. SI Y P, LIU G H, LIU Z Y.Research advances on mechanism and application of probiotics in poultry feed[J].China Poultry, 2016, 38(5):42-46.(in Chinese)
[3] 张玉仙, 王文利, 曹静, 等.不同浓度的同源益生素对肉鸡肌肉品质和组织学性状的影响[J].中国兽医杂志, 2017, 53(7):38-41. ZHANG Y X, WANG W L, CAO J, et al.Effects of different concentrations of homologous probiotics on muscle quality and histological properties of broilers[J].Chinese Journal of Veterinary Medicine, 2017, 53(7):38-41.(in Chinese)
[4] 赵旭, 丁晓, 杨在宾, 等.丁酸梭菌对肉鸡腿肌脂肪代谢的影响[J].动物营养学报, 2017, 29(8):2884-2892. ZHAO X, DING X, YANG Z B, et al.Effects of Clostridium butyricum on thigh muscle lipid metabolism of broilers[J].Chinese Journal of Animal Nutrition, 2017, 29(8):2884-2892.(in Chinese)
[5] 赵旭, 徐群, 侯彦茹, 等.ANGPTL4在肠道微生物影响动物脂肪代谢中的作用[J].生物技术通报, 2020, 36(6):230-235. ZHAO X, XU Q, HOU Y R, et al.Effects of ANGPTL4 on intestinal microbiota affecting lipid metabolism of animals[J].Biotechnology Bulletin, 2020, 36(6):230-235.(in Chinese)
[6] ZHAO X, HUANG H S, DING X, et al.Angiopoietin-like protein 4 regulates breast muscle lipid metabolism in broilers[J].Poultry Science, 2021, 100(7):101159.
[7] ZHANG B, YANG X, GUO Y, et al.Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens[J].Animal, 2011, 5(12):1909-1915.  
[8] WEN C, CHEN X, CHEN G Y, et al.Methionine improves breast muscle growth and alters myogenic gene expression in broilers[J].Journal of Animal Science, 2014, 92(3):1068-1073.  
[9] 张力莉, 徐晓锋.动物脂肪酸合成酶基因表达调控的研究进展[J].畜牧与兽医, 2012, 44(4):101-103. ZHANG L L, XU X F.Research progress in regulation of fatty acid synthase gene expression in animals[J].Animal Husbandry & Veterinary Medicine, 2012, 44(4):101-103.(in Chinese)
[10] 王红, 石宝明.多不饱和脂肪酸对脂肪代谢酶基因表达的影响[J].中国饲料, 2012(1):5-8. WANG H, SHI B M.Effects of polyunsaturated fatty acid on gene expression of lipid metabolism enzyme[J].China Feed, 2012(1):5-8.(in Chinese)
[11] 郑贵花, 夏广军, 尹宝珍.乙酰辅酶A羧化酶在动物中的研究进展[J].现代农业研究, 2019(10):109-112, 120. ZHENG G H, XIA G J, YIN B Z.Advances in acetyl CoA carboxylase in animals[J].Modern Agriculture Research, 2019(10):109-112, 120.(in Chinese)
[12] 杜学海.影响肉牛肌内脂肪沉积因素及相关基因的研究进展[J].现代畜牧兽医, 2020(12):54-57. DU X H.Brief introduction to the research progress of factors and related genes influencing intramuscular fat deposition in beef cattle[J].Modern Journal of Animal Husbandry and Veterinary Medicine, 2020(12):54-57.(in Chinese)
[13] 罗娅, 齐先梅, 王婧.苹果酸酶1及其对疾病调控的研究进展[J].生理科学进展, 2021, 52(3):181-186. LUO Y, QI X M, WANG J.Advances in malic enzyme 1 and its functions on disease regulation[J].Progress in Physiological Sciences, 2021, 52(3):181-186.(in Chinese)
[14] 胡顺霖, 李敏, 叶江锋, 等.非酒精性脂肪肝患者载脂蛋白B及尿酸水平检测结果分析[J].实用临床医药杂志, 2019, 23(8):25-27. HU S L, LI M, YE J F, et al.Analysis in detection results of apolipoprotein B and uric acid levels in patients with non-alcoholic fatty liver disease[J].Journal of Clinical Medicine in Practice, 2019, 23(8):25-27.(in Chinese)
[15] 郭明秋, 殷晓捷, 刁殿琰, 等.脂质代谢水平与冠状动脉粥样硬化病变的关系[J].中国动脉硬化杂志, 2021, 29(2):149-155. GUO M Q, YIN X J, DIAO D Y, et al.Relationship between the levels of lipid metabolism and coronary atherosclerotic lesions[J].Chinese Journal of Arteriosclerosis, 2021, 29(2):149-155.(in Chinese)
[16] 刘黎, 欧阳冬生.微粒体甘油三酯转移蛋白的功能及临床意义[J].国际病理科学与临床杂志, 2009, 29(2):152-155. LIU L, OUYANG D S.Function and clinical significance of microsomal triglyceride transfer proteins[J].International Journal of Pathology and Clinical Medicine, 2009, 29(2):152-155.(in Chinese)
[17] MANDARD S, ZANDBERGEN F, VAN STRATEN E, et al.The fasting-induced adipose factor/angiopoietin-like protein 4 is physically associated with lipoproteins and governs plasma lipid levels and adiposity[J].The Journal of Biological Chemistry, 2006, 281(2):934-944.  
[18] XU A M, LAM M C, CHAN K W, et al.Angiopoietin-like protein 4 decreases blood glucose and improves glucose tolerance but induces hyperlipidemia and hepatic steatosis in mice[J].Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(17):6086-6091.  
[19] 黄海, 米杨, 田苗苗, 等.膳食脂肪的餐后代谢与脂蛋白脂肪酶研究进展[J].食品科学, 2015, 36(9):235-241. HUANG H, MI Y, TIAN M M, et al.Metabolism of postprandial dietary fat and lipoprotein lipase[J].Food Science, 2015, 36(9):235-241.(in Chinese)
[20] LEI X, SHI F J, BASU D, et al.Proteolytic processing of angiopoietin-like protein 4 by proprotein convertases modulates its inhibitory effects on lipoprotein lipase activity[J].The Journal of Biological Chemistry, 2011, 286(18):15747-15756.  
[21] MATTIJSSEN F, KERSTEN S.Regulation of triglyceride metabolism by Angiopoietin-like proteins[J].Biochimica et Biophysica Acta, 2012, 1821(5):782-789.  
[22] YAU M H, WANG Y, LAM K S L, et al.A highly conserved motif within the NH2-terminal coiled-coil domain of angiopoietin-like protein 4 confers its inhibitory effects on lipoprotein lipase by disrupting the enzyme dimerization[J].The Journal of Biological Chemistry, 2009, 284(18):11942-11952.  
[23] WANG Y, LIU L M, WEI L, et al.Angiopoietin-like protein 4 improves glucose tolerance and insulin resistance but induces liver steatosis in high-fat-diet mice[J].Molecular Medicine Reports, 2016, 14(4):3293-3300.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2