研究论文 RESEARCH PAPER

鸡源耐镉乳酸菌株的分离与鉴定

  • 蒲俊华 ,
  • 刘茵茵 ,
  • 陈大伟 ,
  • 高玉时 ,
  • 陆俊贤 ,
  • 张小燕 ,
  • 唐梦君 ,
  • 周倩 ,
  • 孔令武
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  • 1. 江苏省家禽科学研究所, 扬州 225125;
    2. 扬州双扬生物科技有限公司, 扬州 225124
蒲俊华(1975-),女,四川蒲江人,副研究员,博士,研究方向为家禽营养与禽产品质量安全。E-mail:apu123@sina.com

收稿日期: 2021-07-11

  网络出版日期: 2022-02-15

基金资助

国家重点研发计划(2016YFD0501208);江苏现代农业产业技术体系建设专项资金资助(JATS[2021]399);江苏现代农业产业关键技术创新[CX(21)2011];江苏省家禽遗传育种重点实验室项目(JQLAB-ZZ-201707);扬州市现代农业项目(YZ2020049);扬州市社会发展项目(YZ2020061)

Isolation and Identification of Cadmium-Tolerant Lactobacillus Strains from Chicken

  • PU Junhua ,
  • LIU Yinyin ,
  • CHEN Dawei ,
  • GAO Yushi ,
  • LU Junxian ,
  • ZHANG Xiaoyan ,
  • TANG Mengjun ,
  • ZHOU Qian ,
  • KONG Lingwu
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  • 1. Jiangsu Institute of Poultry Sciences, Yangzhou 225125, China;
    2. Yangzhou Shuangyang Biotechnology Co., Ltd., Yangzhou 225124, China

Received date: 2021-07-11

  Online published: 2022-02-15

摘要

本试验旨在从鸡肠道中分离筛选出耐镉(Cd)的乳酸菌,为将其开发应用于防控家禽饲料重金属Cd污染提供理论基础。取鸡肠道内容物的样品进行乳酸菌分离,以耐受100 mg/L的Cd为标准筛选乳酸菌,采用分子生物学方法鉴定菌株。对筛选出的乳酸菌株进行生长性能、耐Cd性能及Cd吸附性能等测定。结果表明:本试验共筛选出5株耐Cd乳酸菌,分别为1株约氏乳杆菌Cd3-1和4株卷曲乳杆菌Cd1-1、Cd5-2、Cd4-5和Cd8-2。其中Cd3-1、Cd5-2和Cd8-2菌株最高可耐受1 000 mg/L的Cd,而Cd1-1和Cd4-5菌株最高可耐受400 mg/L的Cd。各菌株对50 mg/L Cd的吸附率随溶液pH的升高而增加,在pH为6.0和7.0时Cd的吸附率均显著高于pH为2.6时Cd的吸附率(P<0.05)。5株乳酸菌均可耐受0.3%的胆盐,且可抑制金黄色葡萄球菌的生长。综合上述结果,本试验从鸡肠道中分离筛选鉴定出5株耐Cd乳酸菌,包括1株约氏乳杆菌和4株卷曲乳杆菌,在pH接近中性的条件下,对Cd的吸附率可达30%。

关键词: ; ; 乳酸菌

本文引用格式

蒲俊华 , 刘茵茵 , 陈大伟 , 高玉时 , 陆俊贤 , 张小燕 , 唐梦君 , 周倩 , 孔令武 . 鸡源耐镉乳酸菌株的分离与鉴定[J]. 动物营养学报, 2022 , 34(2) : 1305 -1312 . DOI: 10.3969/j.issn.1006-267x.2022.02.062

Abstract

The aim of this experiment was to isolate and screen cadmium(Cd)-tolerant Lactobacillus from the chicken intestine, and to provide a theoretical basis for the prevention and control of Cd contamination used in poultry feed. Lactobacillus, which was isolated and screened from the samples of chicken intestinal contents by resisting 100 mg/L Cd, was identified by molecular biological methods. The growth properties, Cd resistance and Cd adsorption properties were determined on the selected lactic acid strains. The results showed as follows:five Cd-tolerant Lactobacillus strains were identified, including one strain of Lactobacillus johnsonii Cd3-1 and four strains of Lactobacillus crispatus:Cd1-1, Cd5-2, Cd4-5 and Cd8-2. Among these strains, Cd3-1, Cd5-2 and Cd8-2 strains were tolerant up to 1 000 mg/L of Cd while Cd1-1 and Cd4-5 strains were tolerant to 400 mg/L of Cd. The absorption rate of Cd in 50 mg/L Cd solution raised with the increase of pH in the solution, and the Cd absorption rate of each strain in Cd solution of pH 6.0 and 7.0 was significantly higher than that of in Cd solution of pH 2.6. All five Lactobacillus strains were resistant to 0.3% bile salts and inhibited the growth of Staphylococcus aureus. In conclusion, five Cd-tolerant Lactobacillus strains, including one strain of Lactobacillus johnsoni and four strains of Lactobacillus crispatus, are selected from the chicken intestine and their adsorption rates of Cd are up to 30% under the pH close to neutral condition.

Key words: chicken; cadmium; Lactobacillus

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