综述 REVIEW

CD36的棕榈酰化对动物脂肪沉积的调控作用及其机制

  • 符一鸣 ,
  • 郎立敏 ,
  • 江青艳 ,
  • 王松波
展开
  • 华南农业大学动物科学学院, 广东省动物营养调控重点实验室, 优百特脂立方功能性脂肪酸研究中心, 广州 510642
符一鸣(1999-),女,海南屯昌人,硕士研究生,从事脂肪沉积营养调控研究。E-mail:282367883@qq.com

收稿日期: 2021-08-19

  网络出版日期: 2022-03-14

基金资助

国家自然科学基金项目(31972636,32172682)

Regulatory Roles and Mechanisms of CD36 Palmitoylation in Animal Fat Deposition

  • FU Yiming ,
  • LANG Limin ,
  • JIANG Qingyan ,
  • WANG Songbo
Expand
  • UBT Lipid Suite Functional Fatty Acids Research Center, Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2021-08-19

  Online published: 2022-03-14

摘要

动物脂肪的过度沉积不仅会降低胴体品质,还会危害动物健康,因此调控动物脂肪沉积对提高产品品质和健康具有重要意义。CD36又称脂肪酸转运酶,可介导脂肪酸的转运和摄取,在脂肪沉积调控中发挥重要作用。棕榈酰化是指16个碳原子的棕榈酸通过硫酯键与蛋白质的氨基酸残基相结合的一种蛋白质翻译后修饰。最新研究表明,CD36的棕榈酰化在脂肪沉积中发挥重要的调控作用。本文主要阐述了CD36的棕榈酰化对动物脂肪、心脏和肝脏等不同组织器官中脂肪沉积的调控作用,并对其中的机制进行初步的探讨,以期为深入了解棕榈酰化修饰对动物机体脂肪沉积的调控作用及其应用提供参考依据。

本文引用格式

符一鸣 , 郎立敏 , 江青艳 , 王松波 . CD36的棕榈酰化对动物脂肪沉积的调控作用及其机制[J]. 动物营养学报, 2022 , 34(3) : 1456 -1463 . DOI: 10.3969/j.issn.1006-267x.2022.03.010

Abstract

Excessive deposition of animal fat not only reduces the carcass quality, but also is detrimental to animal health. Therefore, regulating animal fat deposition is of great significance to improve the products quality and animal health. CD36, also known as fatty acid translocase, can mediate the transport and uptake of fatty acids, playing vital roles in the regulation of fat deposition. Palmitoylation refers to a kind of protein post-translational modification in which palmitic acid of 16 carbon atoms is combined with amino acid residues of the protein through thioester bonds. Recently, it has been shown that CD36 palmitoylation plays an important regulatory role in fat deposition. Thus, this article mainly reviewed the regulatory effects of CD36 palmitoylation on fat deposition in adipose tissue, heart and liver of animals, and preliminary discussed the possible underlying mechanisms. This review provides reference for further understanding about the regulatory role of palmitoylation in fat deposition and its potential application in animals.

参考文献

[1] SU X,ABUMRAD N A.Cellular fatty acid uptake:a pathway under construction[J].Trends in Endocrinology and Metabolism,2009,20(2):72-77.  
[2] POHL J,RING A,EHEHALT R,et al.New concepts of cellular fatty acid uptake:role of fatty acid transport proteins and of caveolae[J].Proceedings of the Nutrition Society,2004,63(2):259-262.  
[3] EHEHALT R,FVLLEKRUG J,POHL J,et al.Translocation of long chain fatty acids across the plasma membrane-lipid rafts and fatty acid transport proteins[J].Molecular and Cellular Biochemistry,2006,284(1/2):135-140.
[4] BONEN A,CAMPBELL S E,BENTON C R,et al.Regulation of fatty acid transport by fatty acid translocase/CD36[J].Proceedings of the Nutrition Society,2004,63(2):245-249.  
[5] ZHAO L,VARGHESE Z,MOORHEAD J F,et al.CD36 and lipid metabolism in the evolution of atherosclerosis[J].British Medical Bulletin,2018,126(1):101-112.  
[6] PUTRI M,SYAMSUNARNO M R A A,ISO T,et al.CD36 is indispensable for thermogenesis under conditions of fasting and cold stress[J].Biochemical and Biophysical Research Communications,2015,457(4):520-525.  
[7] HAJRI T,ABUMRAD N A.Fatty acid transport across membranes:relevance to nutrition and metabolic pathology[J].Annual Review of Nutrition,2002,22:383-415.
[8] NASSIR F,WILSON B,HAN X L,et al.CD36 is important for fatty acid and cholesterol uptake by the proximal but not distal intestine[J].Journal of Biological Chemistry,2007,282(27):19493-19501.  
[9] TAO N,WAGNER S J,LUBLIN D M.CD36 is palmitoylated on both N- and C-terminal cytoplasmic tails[J].Journal of Biological Chemistry,1996,271(37):22315-22320.  
[10] SPINELLI M,FUSCO S,GRASSI C.Nutrient-dependent changes of protein palmitoylation:impact on nuclear enzymes and regulation of gene expression[J].International Journal of Molecular Sciences,2018,19(12):3820.
[11] DE I,SADHUKHAN S.Emerging roles of DHHC-mediated protein S-palmitoylation in physiological and pathophysiological context[J].European Journal of Cell Biology,2018,97(5):319-338.  
[12] CHAMBERLAIN L H,SHIPSTON M J.The physiology of protein S-acylation[J].Physiological Reviews,2015,95(2):341-376.  
[13] CHAROLLAIS J,VAN DER GOOT F G.Palmitoylation of membrane proteins (Review)[J].Molecular Membrane Biology,2009,26(1/2):55-66.
[14] CHISARI M,SAINI D K,KALYANARAMAN V,et al.Shuttling of G protein subunits between the plasma membrane and intracellular membranes[J].Journal of Biological Chemistry,2007,282(33):24092-24098.  
[15] 周维.脂肪组织CD36棕榈酰化对代谢性炎症和胰岛素敏感性的影响[D].硕士学位论文.重庆:重庆医科大学,2016. ZHOU W.The effects of CD36 palmitoylation on metabolic inflammation and insulin sensitivity in adipose tissue[D].Master's Thesis.Chongqing:Chongqing Medical University,2016.(in Chinese)
[16] KATHAYAT R S,CAO Y,ELVIRA P D,et al.Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes[J].Nature Communications,2018,9(1):334.
[17] ZHAO L,ZHANG C,LUO X X,et al.CD36 palmitoylation disrupts free fatty acid metabolism and promotes tissue inflammation in non-alcoholic steatohepatitis[J].Journal of Hepatology,2018,69(3):705-717.  
[18] JOCHEN A,HAYS J.Purification of the major substrate for palmitoylation in rat adipocytes:N-terminal homology with CD36 and evidence for cell surface acylation[J].Journal of Lipid Research,1993,34(10):1783-1792.  
[19] 王沛.CD36棕榈酰化修饰在肝细胞凋亡中的分子机制研究[D].硕士学位论文.重庆:重庆医科大学,2016. WANG P.The molecular mechanisms of CD36 palmitoylation in hepatocyte apoptosis[D].Master's Thesis.Chongqing:Chongqing Medical University,2016.(in Chinese)
[20] WANG J,HAO J W,WANG X,et al.DHHC4 and DHHC5 facilitate fatty acid uptake by palmitoylating and targeting CD36 to the plasma membrane[J].Cell Reports,2019,26(1):209-221.e5.  
[21] HAO J W,WANG J,GUO H L,et al.CD36 facilitates fatty acid uptake by dynamic palmitoylation-regulated endocytosis[J].Nature Communications,2020,11(1):4765.
[22] STEINBUSCH L K M,SCHWENK R W,OUWENS D M,et al.Subcellular trafficking of the substrate transporters GLUT4 and CD36 in cardiomyocytes[J].Cellular and Molecular Life Sciences,2011,68(15):2525-2538.  
[23] JOCHEN A L,HAYS J,MICK G.Inhibitory effects of cerulenin on protein palmitoylation and insulin internalization in rat adipocytes[J].Biochimica et Biophysica Acta:Lipids and Lipid Metabolism,1995,1259(1):65-72.  
[24] HOWIE J,REILLY L,FRASER N J,et al.Substrate recognition by the cell surface palmitoyl transferase DHHC5[J].Proceedings of the National Academy of Sciences of the United States of America,2014,111(49):17534-17539.  
[25] SCHIANCHI F,GLATZ J F C,NAVARRO GASCON A,et al.Putative role of protein palmitoylation in cardiac lipid-induced insulin resistance[J].International Journal of Molecular Sciences,2020,21(24):9438.
[26] THORNE R F,RALSTON K J,DE BOCK C E,et al.Palmitoylation of CD36/FAT regulates the rate of its post-transcriptional processing in the endoplasmic reticulum[J].Biochimica et Biophysica Acta:Molecular Cell Research,2010,1803(11):1298-1307.  
[27] XIONG Z R.CD36棕榈酰化修饰在脂肪性肝炎发生中的作用(英文)[C]//中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年论文集.太原:中国生物化学与分子生物学会,2019:71. XIONG Z R.CD36 palmitoylation in non-alcoholic steatohepatitis (NASH)[C]//Proceedings of the 2019 National Academic Conference and the 40th Anniversary of the Chinese Society of Biochemistry and Molecular Biology.Taiyuan:The Chinese Society of Biochemistry and Molecular Biology,2019:71.(in Chinese)
[28] EHEHALT R,SPARLA R,KULAKSIZ H,et al.Uptake of long chain fatty acids is regulated by dynamic interaction of FAT/CD36 with cholesterol/sphingolipid enriched microdomains (lipid rafts)[J].BMC Cell Biology,2008,9:45.
[29] POHL J,RING A,KORKMAZ U,et al.FAT/CD36-mediated long-chain fatty acid uptake in adipocytes requires plasma membrane rafts[J].Molecular Biology of the Cell,2005,16(1):24-31.  
[30] SAMOVSKI D,SUN J Y,PIETKA T,et al.Regulation of AMPK activation by CD36 links fatty acid uptake to β-oxidation[J].Diabetes,2015,64(2):353-359.  
[31] 张畅.脂肪酸转位酶CD36的表达与棕榈酰化修饰在高脂诱导的肝细胞凋亡中的作用[D].硕士学位论文.重庆:重庆医科大学,2018. ZHANG C.The expression of CD36 and palmitoylation in high-fat induced hepatocyte apoptosis[D].Master's Thesis.Chongqing:Chongqing Medical University,2018.(in Chinese)
[32] WALLIN T,MA Z H,OGATA H,et al.Facilitation of fatty acid uptake by CD36 in insulin-producing cells reduces fatty-acid-induced insulin secretion and glucose regulation of fatty acid oxidation[J].Biochimica et Biophysica Acta:Molecular and Cell Biology of Lipids,2010,1801(2):191-197.  
[33] LUIKEN J J F P,CHANDA D,NABBEN M,et al.Post-translational modifications of CD36(SR-B2):implications for regulation of myocellular fatty acid uptake[J].Biochimica et Biophysica Acta:Molecular Basis of Disease,2016,1862(12):2253-2258.  
文章导航

/