动物营养学报 >

2022 , Vol. 34 >Issue 3: 1495 - 1507

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.03.014

研究论文 RESEARCH PAPER

小檗碱齐墩果酸盐对仔猪生长性能、血清生化指标和肠道健康的影响

  • 包学太 ,
  • 黎育颖 ,
  • 田军权 ,
  • 苏文璇 ,
  • 杨凡 ,
  • 姚康
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  • 1. 中国科学院亚热带农业生态研究所, 动物营养生理与代谢过程湖南省重点实验室, 湖南省畜禽健康养殖工程技术研究中心, 长沙 410125;
    2. 中国科学院大学, 北京 100049
包学太(1995-),男,青海互助人,硕士研究生,动物营养与饲料科学专业。E-mail:1573002747@qq.com

收稿日期: 2021-07-29

  网络出版日期: 2022-03-14

基金资助

中科院百人计划项目（Y451022111）；国家自然科学基金面上项目（31472107）；湖南"湖湘青年科技创新人才"项目（2015RS4053）；湖南"湖南省杰出青年基金"项目（2016JJ1015）；中国科学院亚热带农业生态过程重点实验室开放基金项目（ISA2016101）；泰山产业领军人才项目（tscy20190121）；中国科学院前沿科学重点研究计划（QYZDY-SSW-SMC008）

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Effects of Berberine-Oleanolate on Growth Performance, Serum Biochemical Indices and Intestinal Health of Piglets

  • BAO Xuetai ,
  • LI Yuying ,
  • TIAN Junquan ,
  • SU Wenxuan ,
  • YANG Fan ,
  • YAO Kang
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  • 1. Hunan Provincial Engineering Research Center for Healthy Breeding of Livestock and Poultry, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2021-07-29

  Online published: 2022-03-14

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摘要

本试验旨在研究饲粮中添加不同剂量小檗碱齐墩果酸盐对感染产肠毒素大肠杆菌（ETEC）仔猪生长性能、血清生化指标和肠道健康的影响。选取50头28日龄健康的"杜×长×大"三元杂交断奶仔猪[体重（8.22±0.98）kg]，随机分为5个组，每组10个重复，每个重复1头猪，单栏饲喂。其中，对照组和模型组饲喂基础饲粮，低、中、高剂量组分别饲喂在基础饲粮中添加50、250和500 mg/kg小檗碱齐墩果酸盐的饲粮。试验预试期3 d，正试期18 d。在正试期第15天，模型组和低、中、高剂量组仔猪灌服10 mL浓度为2×109 CFU/mL的ETEC K88菌液，对照组仔猪灌服相同剂量的无菌LB培养液。结果表明：1）第1~14天，即ETEC K88感染前，与模型组相比，中剂量组仔猪平均日采食量（ADFI）显著升高（P<0.05）。第15~18天，即ETEC K88感染后，各组仔猪体重、ADFI、平均日增重（ADG）和料重比（F/G）均无显著差异（P>0.05）。第1~18天，与模型组相比，中剂量组仔猪ADFI显著升高（P<0.05）；高剂量组F/G显著高于模型组和低剂量组（P<0.05）。2）在感染后24 h，与对照组相比，模型组仔猪粪便评分显著升高（P<0.05）；与模型组相比，低、中、高剂量组仔猪粪便评分明显降低，但差异不显著（P>0.05）。而在感染后48 h，与对照组相比，模型组仔猪粪便评分显著升高（P<0.05）；与模型组相比，低、中、高剂量组仔猪粪便评分显著降低（P<0.05）。3）与模型组相比，中、高剂量组仔猪血清中补体4含量以及血液中淋巴细胞和嗜酸性粒细胞数量均显著升高（P<0.05）；与对照组相比，模型组血液中血小板压积显著降低（P<0.05）；高剂量组血清中补体3含量显著高于模型组（P<0.05）。4）模型组仔猪空肠隐窝深度显著高于其他4组（P<0.05）；中、高剂量组回肠绒毛高度显著高于对照组、模型组和低剂量组（P<0.05）。由此可见，饲粮中添加小檗碱齐墩果酸盐能够提高断奶仔猪生长性能，缓解ETEC K88感染导致的仔猪腹泻，降低腹泻对仔猪生长性能、肠道健康和血清生化指标造成的影响，提高腹泻仔猪机体免疫力；且500 mg/kg的添加剂量对仔猪感染ETEC K88后的肠道损伤有较好的治疗效果。

关键词： 小檗碱齐墩果酸盐; 仔猪; 生长性能; ETEC K88; 腹泻

本文引用格式

包学太 , 黎育颖 , 田军权 , 苏文璇 , 杨凡 , 姚康 . 小檗碱齐墩果酸盐对仔猪生长性能、血清生化指标和肠道健康的影响[J]. 动物营养学报, 2022 , 34(3) : 1495 -1507 . DOI: 10.3969/j.issn.1006-267x.2022.03.014

Abstract

This experiment was conducted to investigate the effects of different doses of dietary berberine-oleanolate on growth performance, serum biochemical indices and intestinal health of piglets infected with enterotoxigenic Escherichia coli (ETEC). Fifty 28-day-old healthy "Duroc×Landrace×Yorkshire" crossbred weaned piglets with body weight of (8.22±0.98) kg were randomly divided into 5 groups with 10 replicates per group and 1 pig per replicate fed in a single cage. Pigs in the control group and the model group were fed a basal diet, and those in the low, mid and high dose groups were fed the basal diet supplemented with 50, 250 and 500 mg/kg berberine-oleanolate, respectively. The pre-trial period lasted for 3 days and the trial period lasted for 18 days. On the 15th day of the trial period, piglets in the model group, low, mid and high dose groups were given 10 mL ETEC K88 bacterial solution with a concentration of 2×109 CFU/mL, and piglets in the control group were given the same dose of sterile LB culture solution. The results showed as follows:1) from days 1 to 14, that was, before ETEC K88 infection, the average daily feed intake (ADFI) of piglets in the mid dose group was significantly increased compared with the model group (P<0.05). From days 15 to 18, that was, after ETEC K88 infection, there were no significant differences in body weight, ADFI, average daily gain (ADG) and feed to gain ratio (F/G) among all groups (P>0.05). From days 1 to 18, compared with the model group, the ADFI of piglets in the mid dose group was significantly increased (P<0.05), and the F/G in high dose group was significantly higher than that in the model group and low dose group (P<0.05). 2) At 24 h after infection, the feces scoring of piglets in the model group was significantly increased compared with the control group (P<0.05); compared with the model group, the feces scoring of piglets in low, mid and high dose groups was decreased, but the difference was not significant (P>0.05). At 48 h after infection, the feces scoring of piglets in the model group was significantly higher than that in the control group (P<0.05); compared with the model group, the feces scoring of piglets in low, mid and high dose groups was significantly decreased (P<0.05). 3) Compared with the model group, the serum complement 4 content and the number of lymphocytes and eosinophils in blood of piglets in mid and high dose groups were significantly increased (P<0.05); compared with the control group, the blood platelet hematocrit in the model group was significantly decreased (P<0.05); the serum complement 3 content in the high dose group was significantly higher than that in the model group (P<0.05). 4) The crypt depth in jejunum of piglets in the model group was significantly higher than that in the other four groups (P<0.05); and the villus height in ileum in the mid and high dose groups was significantly higher than that in the control group, model group and low dose group (P<0.05). In conclusion, dietary berberine-oleanolate can improve the growth performance of weaned piglets, alleviate the diarrhea caused by ETEC K88 infection, reduce the effects of diarrhea on growth performance, intestinal health and serum biochemical indices of piglets, and improve the body immunity of piglets with diarrhea. Moreover, the supplemental dose of 500 mg/kg has a good therapeutic effect on intestinal injury of piglets infected with ETEC K88.

Key words： berberine-oleanolate; weaned piglets; growth performance; ETEC K88; diarrhea

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