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不同青贮添加剂对去油芳樟枝叶青贮饲料营养成分、青贮发酵品质和瘤胃体外发酵特性的影响

  • 王隆 ,
  • 李璟怡 ,
  • 欧阳可寒 ,
  • 瞿明仁 ,
  • 吴欢生 ,
  • 许兰娇 ,
  • 杨食堂 ,
  • 曹永强 ,
  • 杨宇玲 ,
  • 梁欢
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  • 1. 江西农业大学, 江西省动物营养重点实验室, 江西省营养饲料开发工程研究中心, 南昌 330045;
    2. 江西农业大学林学院, 国家林草局木本香料(华东)工程技术研究中心, 南昌 330045;
    3. 国家肉牛牦牛产业技术体系高安实验站, 高安 330800;
    4. 国家肉牛牦牛产业技术体系随州实验站, 湖北省随州市随县畜牧技术推广站, 随州 441300
王隆(1996-),男,江西宜春人,硕士研究生,从事动物营养与饲料科学研究。E-mail:wanglong960627@163.com

收稿日期: 2021-08-12

  网络出版日期: 2022-03-14

基金资助

江西省林业局樟树研究专项课题(2020CXZX07-0405);财政部和农业农村部国家现代农业产业技术体系资助(CARS-37);江西省自然科学基金(20202BAB215009)

Effects of Different Silage Additives on Nutrient Composition, Silage Fermentation Quality and Rumen Fermentation Characteristics in Vitro of Deoiling Linalyl Branches and Leaves Silage

  • WANG Long ,
  • LI Jingyi ,
  • OUYANG Kehan ,
  • QU Mingren ,
  • WU Huansheng ,
  • XU Lanjiao ,
  • YANG Shitang ,
  • CAO Yongqiang ,
  • YANG Yuling ,
  • LIANG Huan
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  • 1. Engineering Research Center of Feed Development of Jiangxi Province, Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Agricultural University, Nanchang 330045, China;
    2. Woody Spice(East China) Engineering Technology Research Center of National Forestry and Grass Administration, Forestry College, Jiangxi Agricultural University, Nanchang 330045, China;
    3. National Beef Cattle and Yak Industrial Technology System Gaoan Experimental Station, Gaoan 330800, China;
    4. Animal Husbandry Technology Extension Station of Suixian, Suizhou City, Hubei Province, National Beef and Yak Industrial Technology System Suizhou Experimental Station, Suizhou 441300, China

Received date: 2021-08-12

  Online published: 2022-03-14

摘要

本试验旨在探讨不同青贮添加剂对去油芳樟枝叶青贮饲料营养成分、青贮发酵品质和瘤胃体外发酵特性的影响。采用单因素试验设计,共设4个组:对照组(不添加任何青贮添加剂)、葡萄糖组(添加20 g/kg葡萄糖)、混合有机酸组(按照7:1:2的体积比例将甲酸、乙酸和丙酸混合,添加6 mL/kg)和纤维素酶组(添加2 g/kg纤维素酶),各青贮添加剂的添加剂量均以青贮原料鲜重为基础,每组设4个重复。青贮45 d后,测定去油芳樟枝叶青贮饲料的营养价值、青贮发酵品质和瘤胃体外发酵特性。结果表明:1)混合有机酸组的粗蛋白质和粗脂肪含量显著高于其他各组(P<0.05);葡萄糖组的粗蛋白质含量显著高于对照组和纤维素酶组(P<0.05),粗灰分含量显著低于其他各组(P<0.05)。2)纤维素酶组的有机酸含量显著高于对照组(P<0.05),混合有机酸组的总碳水化合物含量显著低于其他各组(P<0.05),纤维素酶组的中性洗涤可溶纤维含量显著低于对照组(P<0.05),葡萄糖组的可溶性糖含量显著高于其他各组(P<0.05)。3)纤维素酶组的pH显著低于其他各组(P<0.05),乳酸含量显著高于混合有机酸组和葡萄糖组(P<0.05),总挥发性脂肪酸和乙酸含量显著高于其他各组(P<0.05)。混合有机酸组的总挥发性脂肪酸含量显著高于对照组(P<0.05)。混合有机酸组和葡萄糖组的丙酸含量显著高于对照组和纤维素酶组(P<0.05)。4)葡萄糖组的瘤胃体外发酵培养液pH显著低于其他各组(P<0.05)。葡萄糖组的瘤胃体外发酵培养液体外干物质消化率、微生物蛋白和总挥发性脂肪酸含量均为最高,其中,葡萄糖组的外干物质消化率显著高于混合有机酸组(P<0.05),微生物蛋白和总挥发性脂肪酸含量显著高于对照组和混合有机酸组(P<0.05)。综合以上各项指标进行评价,各组去油芳樟枝叶青贮饲料的综合营养价值由低到高的排序为:对照组<混合有机酸组<纤维素酶组<葡萄糖组。

本文引用格式

王隆 , 李璟怡 , 欧阳可寒 , 瞿明仁 , 吴欢生 , 许兰娇 , 杨食堂 , 曹永强 , 杨宇玲 , 梁欢 . 不同青贮添加剂对去油芳樟枝叶青贮饲料营养成分、青贮发酵品质和瘤胃体外发酵特性的影响[J]. 动物营养学报, 2022 , 34(3) : 1789 -1799 . DOI: 10.3969/j.issn.1006-267x.2022.03.039

Abstract

The purpose of this experiment was to investigate the effects of different silage additives on nutrient composition, silage fermentation quality and rumen fermentation characteristics in vitro of deoiling linalyl branches and leaves silage. Adopting a single factor experiment design, there were 4 groups:control group (without any silage additive), glucose group (added 20 g/kg glucose), mixed organic acid group (formic acid, acetic acid and propionic acid mixed with a volume ratio of 7:1:2, added 6 mL/kg) and cellulase group (added 2 g/kg cellulase). The additive dosage of each silage additive was based on the fresh weight of the silage raw material, each group contained 4 replicates. After 45 days of silage, the nutrient composition, silage fermentation quality and rumen fermentation characteristics in vitro of the deoiling linalyl branches and leaves silage were determined. The results showed as follows:1) the crude protein and ether extract contents of the mixed organic acid group were significantly higher than those of other groups (P<0.05); the crude protein content of the glucose group was significantly higher than that of control group and cellulase group (P<0.05), and the crude ash content was significantly higher than that of other groups (P<0.05). 2) The organic acid content of the cellulase group was significantly higher than that of control group (P<0.05), the total carbohydrate content of the mixed organic acid group was significantly lower than that of other groups (P<0.05), the neutral detergent soluble fiber content of the cellulase group was significantly lower than that of control group (P<0.05), and the soluble sugar content of the glucose group was significantly higher than that of other groups (P<0.05). 3) The pH of the cellulase group was significantly lower than that of other groups (P<0.05), the lactic acid content was significantly lower than that of mixed organic acid group and glucose group (P<0.05), and the total volatile fatty acid and acetic acid contents were significantly higher than those of other groups (P<0.05). The total volatile fatty acid content of the mixed organic acid group was significantly higher than that of control group (P<0.05). The propionic acid content of mixed organic acid group and glucose group was significantly higher than that of control group and cellulase group (P<0.05). 4) The pH in rumen fermentation broth in vitro of glucose group was significantly lower than that of other groups (P<0.05). The in vitro dry matter digestibility, microbial protein and total volatile fatty acid contents in rumen fermentation broth in vitro of the glucose group were all the highest, among them, the in vitro dry matter digestibility of the glucose group was significantly higher than that of the mixed organic acid group (P<0.05), and the microbial protein and total volatile fatty acid contents were significantly higher than those of control group and mixed organic acid group (P<0.05). Comprehensive evaluation of the above indicators, the comprehensive nutritional value of the deoiled linalwood branch and leaf silage in each group is ranked from low to high:control group

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