研究论文 RESEARCH PAPER

米糠和乳酸菌制剂对柠条锦鸡儿青贮发酵品质及微生物多样性的影响

  • 张欢 ,
  • 朱鸿福 ,
  • 闫艳红 ,
  • 张桂杰
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  • 1. 宁夏大学动物科学系, 银川 750021;
    2. 宁夏草牧业工程技术研究中心, 银川 750021;
    3. 四川农业大学动物科技学院, 成都 611130
张欢(1996-),女,宁夏青铜峡人,硕士研究生,研究方向为饲草加工与利用。E-mail:479592543@qq.com

收稿日期: 2021-08-18

  网络出版日期: 2022-03-14

基金资助

农牧交错带牛羊牧繁农育关键技术集成示范项目(16200158-08);宁夏高等学校一流学科建设(草学学科)资助项目(NXYLXK2017A01)

Effects of Rice Bran and Lactic Acid Bacteria Preparation on Fermentation Quality and Microbial Diversity of Caragana korshinskii Silage

  • ZHANG Huan ,
  • ZHU Hongfu ,
  • YAN Yanhong ,
  • ZHANG Guijie
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  • 1. Department of Animal Science, Ningxia University, Yinchuan 750021, China;
    2. Ningxia Grassland and Animal Husbandry Engineering Technology Research Center, Yinchuan 750021, China;
    3. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2021-08-18

  Online published: 2022-03-14

摘要

本试验旨在研究米糠和乳酸菌制剂对柠条锦鸡儿青贮发酵品质及微生物多样性的影响,以期为改善柠条锦鸡儿青贮发酵品质和解决干旱地区饲草料短缺问题提供科学依据。试验设4个处理,分别为无添加(对照组)、添加5%米糠(RB组)、添加5%米糠+105 CFU/g商业植物乳杆菌(RB+LP组)和添加5%米糠+105 CFU/g植物乳杆菌LP694(RB+LP694组)。发酵60 d后,测定柠条锦鸡儿青贮的营养成分、发酵品质及微生物多样性。结果表明:1)与对照组相比,RB、RB+LP和RB+LP694组青贮饲料中性洗涤纤维和酸性洗涤纤维含量均显著降低(P<0.05),RB和RB+LP694组青贮饲料中pH显著降低(P<0.05),RB+LP694组青贮饲料中氨态氮/总氮(NH3-N/TN)显著降低(P<0.05),RB、RB+LP和RB+LP694组青贮饲料中乳酸含量及乳酸/乙酸均显著提高(P<0.05)。2)RB+LP组Shannon指数和Chao1指数显著高于对照、RB和RB+LP694组(P<0.05)。3)各组青贮饲料中优势菌门均为厚壁菌门(Firmicutes),其次为变形菌门(Proteobacteria);优势菌属均为乳杆菌属(Lactobacillus)。综上所述,本试验中,添加5%米糠+105 CFU/g植物乳杆菌LP694改善柠条锦鸡儿青贮发酵品质和微生物组成的效果最好。

本文引用格式

张欢 , 朱鸿福 , 闫艳红 , 张桂杰 . 米糠和乳酸菌制剂对柠条锦鸡儿青贮发酵品质及微生物多样性的影响[J]. 动物营养学报, 2022 , 34(3) : 1800 -1808 . DOI: 10.3969/j.issn.1006-267x.2022.03.040

Abstract

This experiment was conducted to study the effects of rice bran and lactic acid bacteria preparation on fermentation quality and microbial diversity of Caragana korshinskii silage, and to provide scientific basis on improve the fermentation quality of Caragana korshinskii silage and tackle the issue of forage shortage in arid areas. The experiment set 4 treatments:no addition (control group), added 5% rice bran (RB group), added 5% rice bran+105 CFU/g commercial Lactobacillus plantarum (RB+LP group) and added 5% rice bran+105 CFU/g Lactobacillus plantarum LP694 (RB+LP694 group). After 60 days of fermentation, the nutrient composition, fermentation quality and microbial diversity of Caragana korshinskii silage were determined. The results showed as follows:1) compared with the control group, the contents of neutral detergent fiber and acid detergent fiber in silage of RB, RB+LP and RB+LP694 groups were significantly decreased (P<0.05), the pH in silage of RB and RB+LP694 groups was significantly decreased (P<0.05), the ammonia nitrogen/total nitrogen (NH3-N/TN) in silage of RB+LP694 group was significantly decreased (P<0.05), and the lactic acid content and lactic acid/acetic acid in silage of RB, RB+LP and RB+LP694 groups were significantly increased (P<0.05). 2) The Shannon index and Chao1 index of RB+LP group were significantly higher than those of control RB, and RB+LP694 groups (P<0.05). 3) The dominant phyla in 4 groups were Firmicutes, followed by Proteobacteria; and the dominant genus was Lactobacillus. In conclusion, in this experiment, adding 5% rice bran+105 CFU/g Lactobacillus plantarum LP694 has the best effect on improving fermentation quality and microbial composition of Caragana korshinskii silage.

参考文献

[1] ZHANG D Z,SHI Y,HE D H,et al.Responses of Etiella zinckenella to habitat fragmentation of Caraganas in desert steppe in Ningxia,China[J].Acta Ecologica Sinica,2010,30(6):319-326.  
[2] YANG Y,LIU B R.Effects of planting Caragana shrubs on soil nutrients and stoichiometries in desert steppe of Northwest China[J].Catena,2019,183:104213.
[3] 苏海龙.柠条青贮饲料的制作与饲喂效果分析[J].现代农业科技,2018(4):220-221. SU H L.The analysis of preparation and feeding in Caragana silage[J].Modern Agricultural Science and Technology,2018(4):220-221.(in Chinese)
[4] 左忠,张浩,王峰,等.柠条饲料加工利用技术研究[J].草业科学,2005,22(3):30-35. ZUO Z,ZHANG H,WANG F,et al.Discission of processing and utilization techniques of Caragana microphylla feed[J].Pratacultural Science,2005,22(3):30-35.(in Chinese)
[5] 高优娜.鄂尔多斯高原锦鸡儿属几个种的营养价值与饲用价值研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2006:5-6. GAO Y N.Study on the nutritive value and feeding value of several Caragana species in Ordos Plateau[D].Master's Thesis.Huhhot:Inner Mongolia Agricultural University,2006:5-6.(in Chinese)
[6] 弓剑.不同加工处理的柠条锦鸡儿对绵羊生产性能的影响[J].粮食与饲料工业,2012(9):47-49. GONG J.Effects of different processed peashrub on the performance of sheep[J].Cereal & Feed Industry,2012(9):47-49.(in Chinese)
[7] 王保平.柠条青贮调制技术及柠条饲料在奶牛日粮中应用的研究[D].博士学位论文.晋中:山西农业大学,2014:19-20. WANG B P.Studies of the modulation technique of Caragana korshinskii silage and the utilization of Caragana korshinskii in dairy cows diet[D].Ph.D.Thesis.Jinzhong:Shanxi Agricultural University,2014:19-20.(in Chinese)
[8] NKOSI B D,MEESKE R,LANGA T,et al.Effects of ensiling forage soybean (Glycine max (L.) Merr.) with or without bacterial inoculants on the fermentation characteristics,aerobic stability and nutrient digestion of the silage by Damara rams[J].Small Ruminant Research,2016,134:90-96.
[9] BASSO F C,RABELO C H S,LARA E C,et al.Effects of Lactobacillus buchneri NCIMB 40788 and forage:concentrate ratio on the growth performance of finishing feedlot lambs fed maize silage[J].Animal Feed Science and Technology,2018,244:104-115.
[10] YAN Y H,LI X M,GUAN H,et al.Microbial community and fermentation characteristic of Italian ryegrass silage prepared with corn stover and lactic acid bacteria[J].Bioresource Technology,2019,279:166-173.
[11] SHI C X,LIU Z Y,SHI M,et al.Prediction of digestible and metabolizable energy content of rice bran fed to growing pigs[J].Asian-Australasian Journal of Animal Sciences,2015,28(5):654-661.  
[12] LIU Q,ZHOU D Y,CHEN L,et al.Effects of feruloyl esterase,non-starch polysaccharide degrading enzymes,phytase,and their combinations on in vitro degradation of rice bran and nutrient digestibility of rice bran based diets in adult cockerels[J].Livestock Science,2015,178:255-262.
[13] ONO H,NISHIO S,TSURII J,et al.Monitoring of the microbiota profile in nukadoko,a naturally fermented rice bran bed for pickling vegetables[J].Journal of Bioscience and Bioengineering,2014,118(5):520-525.  
[14] 赵娜,杨雪海,魏金涛,等.不同碳水化合物源对饲料油菜青贮品质的影响[J].中国油料作物学报,2021,43(2):236-240. ZHAO N,YANG X H,WEI J T,et al.Effects of different carbohydrate sources on quality of forage rape silage[J].Chinese Journal of Oil Crop Sciences,2021,43(2):236-240.(in Chinese)
[15] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:48-171. ZHANG L Y.Feed analysis and feed quality testing technology[M].3rd ed.Beijing:China Agricultural University Press,2007:48-171.(in Chinese)
[16] 李富国.青贮水稻秸发酵品质及其饲喂肉牛效果的研究[D].硕士学位论文.哈尔滨:东北农业大学,2013:35-36. LI F G.Studies of rice straw silage and its feeding value on beef cattle[D].Master's Thesis.Harbin:Northeast Agricultural University,2013:35-36.(in Chinese)
[17] BRODERICK G A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.  
[18] 张欢,雷晓青,朱鸿福,等.宁夏黄灌区紫花苜蓿与高丹草适宜混贮比例研究[J].中国草地学报,2020,42(5):136-142. ZHANG H,LEI X Q,ZHU H F,et al.Study on quality of mixed silage of alfalfa and sorghum-sudangrass hybrid silage in Yellow River irrigation region of Ningxia[J].Chinese Journal of Grassland,2020,42(5):136-142.(in Chinese)
[19] 张增欣,邵涛.丙酸对多花黑麦草青贮发酵动态变化的影响[J].草业学报,2009,18(2):102-107. ZHANG Z X,SHAO T.The effect of propionic acid addition on the dynamic fermentation changes of Italian ryegrass (Lolium multiflorum) silage[J].Acta Prataculturae Sinica,2009,18(2):102-107.(in Chinese)
[20] GUO Y L,HAN H Z,LUO R R,et al.Determining the ensiling characteristics of potato vine silage supplemented with rice bran and corn and evaluating their ruminal fermentation potential in vitro[J].New Zealand Journal of Agricultural Research,2020,63(2):179-188.  
[21] YUAN X J,DONG Z H,LI J F,et al.Microbial community dynamics and their contributions to organic acid production during the early stage of the ensiling of Napier grass (Pennisetum purpureum)[J].Grass and Forage Science,2020,75(1):37-44.  
[22] MADIGAN M T,BENDER K S,BUCKLEY D H,et al.Brock biology of microorganisms[M].15th ed.New York:Pearson,2017.
[23] 宋兆齐,王莉,刘秀花,等.云南和西藏四处热泉中的厚壁菌门多样性[J].生物技术,2015,25(5):481-486,436. SONG Z Q,WANG L,LIU X H,et al.Diversities of firmicutes in four hot springs in Yunnan and Tibet[J].Biotechnology,2015,25(5):481-486,436.(in Chinese)
[24] GUAN H,SHUAI Y,RAN Q F,et al.The microbiome and metabolome of Napier grass silages prepared with screened lactic acid bacteria during ensiling and aerobic exposure[J].Animal Feed Science and Technology,2020,269:114673.
[25] 倪奎奎.全株水稻青贮饲料中微生物菌群以及发酵品质分析[D].博士学位论文.郑州:郑州大学,2016:15. NI K K.Research on the bacterial community and fermentation quality of whole crop rice silage[D].Ph.D.Thesis.Zhengzhou:Zhengzhou University,2016:15.(in Chinese)
[26] WANG S R,ZHAO J,DONG Z H,et al.Sequencing and microbiota transplantation to determine the role of microbiota on the fermentation type of oat silage[J].Bioresource Technology,2020,309:123371.
[27] SILVA V P,PEREIRA O G,LEANDRO E S,et al.Effects of lactic acid bacteria with bacteriocinogenic potential on the fermentation profile and chemical composition of alfalfa silage in tropical conditions[J].Journal of Dairy Science,2016,99(3):1895-1902.  
[28] SANTOS A O,ÁVILA C L S,PINTO J C,et al.Fermentative profile and bacterial diversity of corn silages inoculated with new tropical lactic acid bacteria[J].Journal of Applied Microbiology,2016,120(2):266-279.  
[29] CAI Y,BENNO Y,OGAWA M,et al.Influence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation[J].Applied and Environmental Microbiology,1998,64(8):2982-2987.  
[30] 赵嫚,陆永祥,陈仕勇,等.添加剂对成都平原玉米秸秆发酵品质和细菌群落组成的影响[J].草学,2021(3):48-54. ZHAO M,LU Y X,CHEN S Y,et al.Effects of additives on fermentation quality and bacterial community composition of maize straw in Chengdu plain[J].Journal of Grassland and Forage Science,2021(3):48-54.(in Chinese)
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