研究论文 RESEARCH PAPER

菊粉对克氏原螯虾消化酶活性、肠道组织形态和非特异性免疫能力的影响

  • 付运银 ,
  • 范文浩 ,
  • 阮国良 ,
  • 何乃娟 ,
  • 李生瑄 ,
  • 李青松 ,
  • 方刘 ,
  • 王乾
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  • 长江大学小龙虾繁育与健康养殖创新技术研究中心, 荆州 434025
付运银(1996-),女,四川广安人,硕士研究生,从事淡水甲壳动物营养研究。E-mail:1183845277@qq.com

收稿日期: 2021-08-12

  网络出版日期: 2022-03-14

基金资助

湖北省中央引导地方科技发展专项(2019ZYYD035);荆州市2019年度科技计划(2019EC61-11)

Effects of Inulin on Digestive Enzyme Activities, Intestinal Morphology and Non-Specific Immunity of Procambarus clarkii

  • FU Yunyin ,
  • FAN Wenhao ,
  • RUAN Guoliang ,
  • HE Naijuan ,
  • LI Shengxuan ,
  • LI Qingsong ,
  • FANG Liu ,
  • WANG Qian
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  • The Innovative Technology Research Center of Crayfish Breeding and Healthy Farming, Yangtze University, Jingzhou 434025, China

Received date: 2021-08-12

  Online published: 2022-03-14

摘要

本试验旨在研究饲料中添加不同水平菊粉对克氏原螯虾消化酶活性、肠道组织形态和非特异性免疫能力的影响。试验选用240尾平均体重为(6.58±0.16)g的克氏原螯虾,随机分为6个组,分别饲喂在基础饲料中添加0(对照组)、0.20%、0.40%、0.60%、0.80%和1.00%菊粉的饲料,每组4个重复,养殖密度为10尾/箱。试验期7周,试验结束后测定胃组织中的消化酶活性和肝胰腺中的免疫酶活性,并用显微镜观察后肠的组织形态。结果表明:1)与对照组相比,0.40%~1.00%菊粉添加组胃组织中淀粉酶、脂肪酶和蛋白酶活性显著提高(P<0.05);其中,0.60%菊粉添加组胃组织中蛋白酶活性最高,0.80%菊粉添加组胃组织中淀粉酶和脂肪酶活性最高。2)与对照组相比,随着饲料中菊粉添加水平的提高,后肠黏膜的皱襞数量增加,结缔组织更为紧密,肌层更加明显和完整;其中,0.40%~1.00%菊粉添加组后肠黏膜皱襞长度和宽度显著高于对照组(P<0.05)。3)与对照组相比,0.40%~1.00%菊粉添加组肝胰腺碱性磷酸酶、酸性磷酸酶和溶菌酶活性显著提高(P<0.05),且均以0.60%菊粉添加组活性最高。结果提示,饲料中添加菊粉可以提高克氏原螯虾胃组织中消化酶活性,改善肠道形态结构,增强非特异性免疫能力,且以0.6%添加水平效果较佳。

本文引用格式

付运银 , 范文浩 , 阮国良 , 何乃娟 , 李生瑄 , 李青松 , 方刘 , 王乾 . 菊粉对克氏原螯虾消化酶活性、肠道组织形态和非特异性免疫能力的影响[J]. 动物营养学报, 2022 , 34(3) : 1845 -1852 . DOI: 10.3969/j.issn.1006-267x.2022.03.044

Abstract

This experiment was conducted to investigate the effects of different dietary inulin levels on digestive enzyme activities, intestinal morphology and non-specific immunity of Procambarus clarkii. A total of 240 crayfish with an average body weight of (6.58±0.16) g were randomly divided into 6 groups with 4 replicates in each group and 10 crayfish per box. The crayfish were fed the basal diet supplemented with 0 (control group), 0.20%, 0.40%, 0.60%, 0.80% and 1.00% inulin, respectively. The experiment lasted for 7 weeks. After the experiment, the digestive enzyme activities in stomach tissue and immune enzyme activities in hepatopancreas were determined, and the histological morphology of hind-gut was observed under microscope. The results showed as follows:1) compared with the control group, the activities of amylase, lipase and protease in stomach tissue in 0.40% to 1.00% inulin supplemental groups were significantly increased (P<0.05); among them, the protease activity in stomach tissue in 0.60% inulin supplemental group was the highest, and the amylase and lipase activities in stomach tissue in 0.80% inulin supplemental group were the highest. 2) Compared with the control group, with the increase of inulin supplemental level, the duplicature number in hind-gut was increased, the connective tissue became closer, and the muscle layer became more obvious and complete; the length and width of duplicature in hind-gut in 0.40% to 1.00% inulin supplemental groups were significantly higher than those in the control group (P<0.05). 3) Compared with the control group, the activities of alkaline phosphatase, acid phosphatase and lysozyme in hepatopancreas in 0.40% to 1.00% inulin supplemental groups were significantly increased (P<0.05), and the activities in 0.60% inulin supplemental group were the highest. The results indicate that inulin supplementation can improve the digestive enzyme activities in stomach tissue, improve intestinal morphology and enhance non-specific immunity of Procambarus clarkii, and 0.6% inulin supplementation has a better effect.

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