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人工草场不同饲养模式对羔羊小肠脂肪消化和肝脏脂质代谢相关基因表达的影响

  • 李贞 ,
  • 彭思嘉 ,
  • 左淑贤 ,
  • 王月君 ,
  • 罗海玲 ,
  • 张英俊 ,
  • 王占军
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  • 1. 中国农业大学动物科技学院, 动物营养国家重点实验室, 北京 100193;
    2. 中国农业大学草业科学与技术学院, 北京 100193;
    3. 宁夏农林科学院, 银川 750002
李贞(1994-),女,四川绵阳人,博士研究生,动物营养与饲料科学专业。E-mail:lizhen6394@126.com

收稿日期: 2021-08-12

  网络出版日期: 2022-03-14

基金资助

宁夏回族自治区重点研发项目(2017BY082);国家肉羊产业技术体系(CARS-38)

Effects of Different Feeding Patterns on Intestinal Lipid Digestion and Expression of Hepatic Lipid Metabolism Related Genes of Lambs Feeding on Artificial Pasture

  • LI Zhen ,
  • PENG Sijia ,
  • ZUO Shuxian ,
  • WANG Yuejun ,
  • LUO Hailing ,
  • ZHANG Yingjun ,
  • WANG Zhanjun
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  • 1. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
    2. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
    3. Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China

Received date: 2021-08-12

  Online published: 2022-03-14

摘要

本试验旨在研究人工草场不同饲养模式对羔羊小肠脂肪消化和肝脏脂质代谢相关基因表达的影响。试验选取33只体重[(19.69±0.29)kg]相近的3月龄断奶滩羊公羔,随机分为舍饲组(H组)、人工草场放牧补饲组(GH组)和人工草场放牧组(G组),每组11只。H组单栏饲养,每天饲喂2次;GH组每天放牧4 h,归牧后进行单栏补饲;G组每天放牧12 h不补饲。预试期7 d,正试期90 d。结果表明:不同饲养模式下,G组小肠重量显著高于H组(P<0.05),G组小肠指数显著高于GH组和H组(P<0.05),而十二指肠及回肠的绒毛高度和绒毛高度/隐窝深度均显著低于GH组和H组(P<0.05),但空肠的隐窝深度G组显著高于GH组和H组(P<0.05),GH组和G组空肠的肌层厚度显著高于H组(P<0.05)。H组空肠食糜上清脂肪酶活性显著高于G组(P<0.05)。H组肝脏脂质代谢相关基因肉碱棕榈酰转移酶1(CPT1)、β-羟基-β-甲基戊二酸单酰辅酶A裂解酶(HMGCL)、β-羟基-β-甲基戊二酸单酰辅酶A还原酶(HMGCR)相对表达量显著高于G组(P<0.05)。结果提示,H组和GH组羔羊小肠形态发育更优,H组空肠脂肪酶活性更高,利于脂肪消化;H组羔羊肝脏CPT1、HMGCLHMGCR基因相对表达量显著上调,利于体脂沉积。

本文引用格式

李贞 , 彭思嘉 , 左淑贤 , 王月君 , 罗海玲 , 张英俊 , 王占军 . 人工草场不同饲养模式对羔羊小肠脂肪消化和肝脏脂质代谢相关基因表达的影响[J]. 动物营养学报, 2022 , 34(3) : 1920 -1930 . DOI: 10.3969/j.issn.1006-267x.2022.03.051

Abstract

This study was aimed to investigate the effects of different feeding patterns on small intestinal lipid digestion and liver lipid metabolism of Tan lambs feeding on artificial pasture. Thirty-three Tan male lambs with an average age of 3 months and similarly body weight of (19.6±0.29) kg were selected. Lambs were randomly divided into three groups:housing group (H group), artificial pasture grazing with housing group (GH group) and artificial pasture grazing group (G group), with 11 lambs in each group. The H group was fed twice a day in a single column. The GH group grazed for 4 h a day, and then received single column supplementary feeding after grazing. The G group grazed for 12 h a day without supplementary feeding. The adaptation period was 7 days, and the formal experiment period was 90 days. The results showed that under different feeding patterns, the small intestinal weight of G group was significantly higher than that of H group (P<0.05), and the intestinal index of G group was significantly higher than that of GH group and H group (P<0.05). The villus height and villus height/crypt depth of duodenum and ileum of GH group and H group were significantly higher than those of G group (P<0.05), and the depth of jejunum crypt of G group was significantly higher than that of GH group and H group (P<0.05), and the thickness of jejunum muscle layer of GH group and G group was significantly higher than that of H group (P<0.05). Besides, the activity of lipase in jejunal digesta supernatant of H group was significantly higher than that of G group (P<0.05). The relative expression levels of carnitine palmityl transferase 1 (CPT1), β-hydroxy-β-methylglutaryl-coenzyme A reductase (HMGCL) and β-hydroxy-β-methylglutaryl-coenzyme A lyase (HMGCR) genes related to liver lipid metabolism of H group were significantly higher than those of G group (P<0.05). The results indicate that the intestinal morphology of lambs of the H group and GH group is better, and the jejunal lipase activity of the H group is higher, which is conducive to lipid digestion. The relative expression levels of CPT1, HMGCL and HMGCR genes of the H group are significantly up-regulated, which is beneficial to body lipid deposition.

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