动物营养学报 >

2022 , Vol. 34 >Issue 3: 1963 - 1974

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.03.055

研究论文 RESEARCH PAPER

硼对大鼠肝脏显微结构、肝糖原含量、抗氧化功能及细胞增殖和凋亡相关基因表达的影响

  • 丁永旺 ,
  • 任曼 ,
  • 赵春芳 ,
  • 顾有方 ,
  • 靳二辉 ,
  • 李升和
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  • 1. 安徽科技学院动物科学学院, 凤阳 233100;
    2. 动物营养调控与健康安徽省重点实验室, 凤阳 233100
丁永旺(1998-),男,河南西华人,硕士研究生,研究方向为微量元素营养与健康。E-mail:1786102523@qq.com

收稿日期: 2021-08-23

  网络出版日期: 2022-03-14

基金资助

国家自然科学基金（32172816，32002160）；安徽省自然科学基金（2108085MC117）；安徽省重大科技专项（201903a06020002）；安徽省协同创新项目（GXXT-2019-035）；滁州市科技计划项目（2019ZN003）

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Effects of Boron on Microstructure, Hepatic Glycogen Content, Antioxidant Function, Cell Proliferation and Apoptosis Related Gene Expression in Liver of Rats

  • DING Yongwang ,
  • REN Man ,
  • ZHAO Chunfang ,
  • GU Youfang ,
  • JIN Erhui ,
  • LI Shenghe
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  • 1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China;
    2. Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang 233100, China

Received date: 2021-08-23

  Online published: 2022-03-14

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摘要

本试验旨在研究硼对大鼠肝脏显微结构、肝糖原含量、抗氧化功能及细胞增殖和凋亡相关基因表达的影响。选用100只清洁级健康的（22±2）日龄雄性SD大鼠，适应性7 d后随机分为10组，每组10只。对照组饮用蒸馏水（硼含量为0），试验组分别饮用硼含量为5、10、20、40、80、160、320、480、640 mg/L的蒸馏水。试验期60 d。结果表明：1）与对照组相比，饮水补充5、10和20 mg/L硼可明显改善肝脏显微结构，肝小叶轮廓更加清晰，肝细胞索排列更加整齐，双核肝细胞数量增多，肝枯否氏细胞数量增加，进而改善肝脏显微结构；而饮水补充480和640 mg/L硼则导致肝细胞索排列紊乱，肝血窦变宽，肝细胞核染色加深，肝细胞内出现空泡样结构。2）与对照组相比，饮水补充5和10 mg/L硼极显著提高肝脏中肝糖原含量（P<0.01），显著或极显著提高肝脏超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）（P<0.05或P<0.01），显著或极显著提高肝脏核因子E2相关因子2（Nrf2）和增殖细胞核抗原（PCNA）mRNA相对表达量（P<0.05或P<0.01）；饮水补充480和640 mg/L硼则极显著降低肝脏中肝糖原含量（P<0.01），显著或极显著降低肝脏T-AOC（P<0.05或P<0.01），显著或极显著提高肝脏丙二醛（MDA）含量（P<0.05或P<0.01），极显著提高肝脏B细胞淋巴瘤/白血病-2相关X蛋白（Bax）和半胱天冬蛋白酶-3（Caspase-3）mRNA相对表达量（P<0.01），显著或极显著降低肝脏Nrf2、依赖还原型辅酶Ⅰ（Ⅱ）醌氧化还原酶1（NQO1）、PCNA和B细胞淋巴瘤/白血病-2（Bcl-2）mRNA相对表达量（P<0.05或P<0.01）。由此可见，饮水补充5和10 mg/L硼可明显改善大鼠肝脏显微结构，增加肝糖原含量，提高肝脏抗氧化能力，促进肝脏Nrf2和PCNA mRNA表达，而饮水补充480和640 mg/L硼则产生相反作用。

关键词： 硼; 大鼠; 肝脏; 显微结构; 抗氧化功能; 细胞增殖和凋亡

本文引用格式

丁永旺 , 任曼 , 赵春芳 , 顾有方 , 靳二辉 , 李升和 . 硼对大鼠肝脏显微结构、肝糖原含量、抗氧化功能及细胞增殖和凋亡相关基因表达的影响[J]. 动物营养学报, 2022 , 34(3) : 1963 -1974 . DOI: 10.3969/j.issn.1006-267x.2022.03.055

Abstract

This experiment was conducted to study the effects of boron on microstructure, hepatic glycogen content, antioxidant function and of cell proliferation and apoptosis related gene expression in liver of rats. One hundred clean healthy (22±2)-day-old male SD rats were randomly divided into 10 groups with 10 rats per group after 7 days of adaptive feeding. The control group was drinking distilled water (boron content was 0), and the experimental groups were drinking distilled water which boron contents were 5, 10, 20, 40, 80, 160, 320, 480 and 640 mg/L, respectively. The experiment lasted for 60 days. The results showed as follows:1) compared with the control group, drinking water supplemented with 5, 10 and 20 mg/L boron could obviously improve the liver microstructure, the outline of hepatic lobule was clearer, the arrangement of hepatocyte cord was more orderly, the number of binuclear hepatocytes and Kupffer cells were increased, and then the liver microstructure was improved; while drinking water supplemented with 640 mg/L boron resulted in disordered arrangement of hepatocyte cord, broadening of hepatic sinusoid, deepening staining of hepatocyte nucleus and vacuole-like structure in hepatocyte. 2) Compared with the control group, drinking water supplemented with 5 and 10 mg/L boron significantly increased the content of hepatic glycogen in liver (P<0.01), significantly increased the superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) in liver (P<0.05 or P<0.01), and significantly increased the mRNA relative expression levels of nuclear factor E2-related factor 2 (Nrf2) and proliferating cell nuclear antigen (PCNA) in liver (P<0.05). However, drinking water supplemented with 480 and 640 mg/L boron significantly decreased the content of hepatic glycogen in liver (P<0.01), significantly decreased the liver T-AOC (P<0.05 or P<0.01), significantly increased the liver malondialdehyde (MDA) content (P<0.05 or P<0.01), significantly increased the mRNA relative expression levels of B-cell lymphoma/leukaemia-2-associated X protein (Bax) and cysteinyl aspartate specific proteinase-3 (Caspase-3) in liver (P<0.01), and significantly decreased the mRNA relative expression levels of Nrf2, NAD(P)H:quinine oxidoreductase 1 (NQO1), PCNA and B-cell lymphoma/leukaemia-2 (Bcl-2) in liver (P<0.05 or P<0.01). In conclusion, drinking water supplemented with 5 and 10 mg/L boron can improve the liver microstructure of rats, increase the liver hepatic glycogen content and antioxidant capacity, and promote the liver Nrf2 and PCNA mRNA expression, while drinking water supplemented with 480 and 640 mg/L boron has the opposite effects.

Key words： boron; rats; liver; microstructure; antioxidant function; cell proliferation and apoptosis

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