研究论文 RESEARCH PAPER

基于指纹图谱技术的燕麦草质量评价与相关性验证

  • 潘予琮 ,
  • 寇伟 ,
  • 蒋林树 ,
  • 年芳
展开
  • 1. 北京农学院动物科学技术, 奶牛营养学北京市重点实验室, 北京 102206;
    2. 甘肃农业大学动物科学技术学院, 兰州 730070
潘予琮(1997-),女,北京人,硕士研究生,研究方向为反刍动物营养与免疫。E-mail:443066983@qq.com

收稿日期: 2021-08-21

  网络出版日期: 2022-03-14

基金资助

国家"十三五"重点研发计划(2017YFD0701604-2);北京市现代农业产业技术体系奶牛创新团队

Quality Evaluation and Correlation Verification of Oat Grass Based on Fingerprint Technology

  • PAN Yuzong ,
  • KOU Wei ,
  • JIANG Linshu ,
  • NIAN Fang
Expand
  • 1. Beijing Key Laboratory of Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2021-08-21

  Online published: 2022-03-14

摘要

本试验旨在从黄酮类营养活性物质角度对不同来源燕麦草进行质量评价与相关性验证。以18种不同来源燕麦草(S1~S18)作为研究对象,采用NaNO2-Al(NO33-NaOH显色法测定总黄酮含量并结合高效液相色谱建立燕麦草黄酮特征图谱,利用聚类分析、主成分分析和灰色关联分析进行质量评价与相关性验证。结果显示:不同来源燕麦草中的总黄酮含量存在极显著差异(P<0.01),其中S7总黄酮含量最高(10.151 mg/g),S15总黄酮含量最低(5.503 mg/g);根据燕麦草的特征指纹图谱,结合现代分析技术,将18种不同来源燕麦草分为3类,其中第1类为S15、S17、S5、S3、S16,第2类为S1、S11、S8、S18、S6、S10、S2、S4、S9、S14,第3类为S12、S13、S7;灰色关联度分析结果表明,7个指标对饲喂价值的影响重要程度依次为黄酮含量>干物质含量>粗蛋白质含量>酸性洗涤纤维含量>粗灰分含量>中性洗涤纤维含量>粗脂肪含量,为燕麦草深度开发和奶牛饲粮配方优化提供新思路。

本文引用格式

潘予琮 , 寇伟 , 蒋林树 , 年芳 . 基于指纹图谱技术的燕麦草质量评价与相关性验证[J]. 动物营养学报, 2022 , 34(3) : 1984 -1995 . DOI: 10.3969/j.issn.1006-267x.2022.03.057

Abstract

The aim of this study was to analyze and evaluate the quality of oat grass from different sources from the perspective of flavonoid. In this study, 18 kinds of oat grass from different sources (S1 to S18) were used to determine the content of total flavonoid by NaNO2-Al (NO3)3-NaOH colorimetry and establish the characteristic spectrum of flavonoids nutritional active substances by high performance liquid chromatography. Cluster analysis, principal component analysis and grey correlation analysis were used to evaluate the quality and verify the correlation. The results showed that there were significant differences in flavonoid content among different sources of oat grass, the total flavon content of S7 was the highest (10.151 mg/g), and the total flavon content of S15 was the lowest (5.503 mg/g). According to the characteristic fingerprint of oat grass, 18 kinds of oat species were divided into 3 groups, the first group was S15, S17, S5, S3, S16, the second group was S1, S11, S8, S18, S6, S10, S2, S4, S9, S14 and the third group was S12, S13, S7. The results of grey correlation analysis showed that the important degree of 7 indexes on feeding value is flavonone content>dry matter content>crude protein content>acid detergent fiber content>ash content>neutral detergent fiber content>crude fat content, providing the technical foundation for the deep development of oat grass and the optimization of dairy diet formula.

参考文献

[1] 贺忠勇.燕麦干草在奶牛生产中的优势及应用[J].中国奶牛,2015(17):12-15. HE Z Y.Advantages and application of oatmeal hay in cow production[J].China Dairy Cattle,2015(17):12-15.(in Chinese)
[2] 段中华,乔有明.不同因子对燕麦β-葡聚糖提取纯度影响的研究[J].食品科学,2007,32(8):122-126. DUAN Z H,QIAO Y M.Study of different factors on extraction purity of oat β-glucan[J].Food Science and Technology,2007,32(8):122-126.(in Chinese)
[3] CASSIDY A,MINIHANE A M.The role of metabolism (and the microbiome) in defining the clinical efficacy of dietary flavonoids[J].The American Journal of Clinical Nutrition,2017,105(1):10-22.  
[4] CHEN L Y,TANG X M,YANG Q,et al.Quantitative and chemical fingerprint analysis of Desmodium styracifolium by high-performance liquid chromatography combined with chemometrics[J].Journal of Chromatographic Science,2020,58(4):294-302.  
[5] 潘予琮,蒋林树,熊本海.指纹图谱技术及其在饲料营养活性物质评价中的应用研究进展[J].动物营养学报,2020,32(9):4044-4052. PAN Y C,JIANG L S,XIONG B H.Research progress of fingerprinting technique and its application in evaluation of nutricines in feeds[J].Chinese Journal of Animal Nutrition,2020,32(9):4044-4052.(in Chinese)
[6] 严辉,谢舒平,濮宗进,等.基于UPLC-PDA指纹图谱及多成分含量的化学模式识别法评价大黄质量[J].中草药,2020,51(18):4755-4762. YAN H,XIE S P,PU Z J,et al.Quality evaluation of rhubarb based on UPLC-PDA fingerprint and multi-component content determination by chemical pattern recognition[J].Chinese Traditional and Herbal Drugs,2020,51(18):4755-4762.(in Chinese)
[7] 刘英,周娟,陈冬梅,等.饲料中粗纤维定量测定能力验证结果与分析[J].饲料博览,2020(4):42-45,48. LIU Y,ZHOU J,CHEN D M,et al.Validation results and analysis of quantitative determination ability of crude fiber in feed[J].Feed Review,2020(4):42-45,48.(in Chinese)
[8] ROHWEDER D A,BARNES R F,JORGENSEN N.Proposed hay grading standards based on laboratory analyses for evaluating quality[J].Journal of Animal Science,1978,47(3):747-759.  
[9] 桂利利,吴正坤,余惠凡,等.黄药叶总黄酮提取工艺优化及其抗氧化活性研究[J].食品研究与开发,2021,42(19):100-108. GUI L L,WU Z K,YU H F,et al.Optimization of extraction and antioxidant activity of total flavonoids from Premna cavaleriei Levl. leaves[J].Food Research and Development,2021,42(19):100-108.(in Chinese)
[10] 陈亮,王克勤.芹菜中总黄酮测定方法的探讨[J].中国食品添加剂,2007(2):214-217. CHEN L,WANG K Q.Discussion about the method for determination of flavonids in celery extract[J].China Food Additives,2007(2):214-217.(in Chinese)
[11] 孙雪皎,史琳,张旋,等.沙棘汁总黄酮测定方法的对比分析[J].沈阳农业大学学报,2019,50(1):120-127. SUN X J,SHI L,ZHANG X,et al.Comparison on determining methods for the total flavonoids in sea buckthorn juice[J].Journal of Shenyang Agricultural University,2019,50(1):120-127.(in Chinese)
[12] ZHANG C W,WANG C Z,TAO R.Analysis on the physicochemical properties of Ginkgo biloba leaves after enzymolysis based ultrasound extraction and Soxhlet extraction[J].Molecules,2016,21(1):97.
[13] CHEN S,LI X,LIU X,et al.Investigation of chemical composition,antioxidant activity,and the effects of alfalfa flavonoids on growth performance[J].Oxidative Medicine and Cellular Longevity,2020,2020:8569237.
[14] 韩友文.饲料常规概略养分分析方案[J].饲料博览,2004(2):48. HAN Y W.Routine general nutrient analysis program for feed[J].Feed Review,2004(2):48.(in Chinese)
[15] LU Y Y,GUO S,ZHANG F,et al.Comparison of functional components and antioxidant activity of Lycium barbarum L. fruits from different regions in China[J].Molecules,2019,24(12):2228.
[16] 陈振兴,周凤玲,张仲敏.不同产地酸藤子根总黄酮含量及抗氧化活性研究[J].广西中医药,2021,44(1):62-65. CHEN Z X,ZHOU F L,ZHANG Z M.Study on total Flavonoids content determination and antioxidant activity of Embelia laeta (L.) Mez from different producing areas[J].Guangxi Journal of Traditional Chinese Medicine,2021,44(1):62-65.(in Chinese)
[17] 牛晓方,高一军,怀宝刚,等.不同产地牡丹叶中总黄酮的含量测定[J].菏泽医学专科学校学报,2021,33(1):95-96. NIU X F,GAO Y J,HUAI B G,et al.Determination of total flavonoids in leaves of peony from different areas[J].Journal of Heze Medical College,2021,33(1):95-96.(in Chinese)
[18] 卢德勋.饲料营养活性物质(nutricines):一个亟待重新审视的研究领域[J].饲料工业,2020,41(3):1-5. LU D X.Feed nutricines:a research area that is worth to examine closely[J].Feed Industry,2020,41(3):1-5.(in Chinese)
[19] 荆常亮.紫花苜蓿总黄酮的提取、纯化及其抗氧化活性研究[D].博士学位论文.北京:中国农业科学院,2016. JING C L.Extraction,purification and antioxidant activities of flavonoids from alfalfa[D].Ph.D.Thesis.Beijing:Chinese Academy of Agricultural Sciences,2016.(in Chinese)
[20] 黄晓莉,钟磊,闵远洋,等.不同产地广金钱草种质资源综合评价[J/OL].分子植物育种:1-21[2021-09-22].http://kns.cnki.net/kcms/detail/46.1068.s.20210917.1512.014.html. HUANG X L,ZHONG L,MIN Y Y,et al.Study on comprehensive evaluation of germplasm resources of Desmodium styracifolium from different habitats[J/OL].Molecular Plant Breeding:1-21[2021-09-22].http://kns.cnki.net/kcms/detail/46.1068.s.20210917.1512.014.html. (in Chinese)
[21] 林永明,丰诗尧,胡美静,等.玉米筛选品种9个性状的灰色关联度法综合评价[J].云南农业科技,2015(5):4-6. LIN Y M,FENG S Y,HU M J,et al.Comprehensive evaluation of 9 characters of maize screened varieties by grey correlation degree method[J].Yunnan Agricultural Science and Technology,2015(5):4-6.(in Chinese)
[22] 王建芳,高山,牟德华.基于主成分分析和聚类分析的不同品种燕麦品质评价[J].食品工业科技,2020,41(13):85-91. WANG J F,GAO S,MOU D H.Quality evaluation of different varieties of oat based on principal components analysis and cluster analysis[J].Science and Technology of Food Industry,2020,41(13):85-91.(in Chinese)
[23] 杨玉宁,陈松树,高尔刚,等.3种木通属植物21份种质果实的果皮品质评价[J/OL].中药材,2021(5):1089-1096[2021-06-01].https://doi.org/10.13863/j.issn1001-4454.2021.05.010. YANG Y N,CHEN S S,GAO E G,et al.Quality evaluation of pericarp and fruits of twenty-one germplasms in three Akebia plants[J/OL].Journal of Chinese Medicinal Materials,2021(5):1089-1096[2021-06-01].https://doi.org/10.13863/j.issn1001-4454.2021.05.010. (in Chinese)
文章导航

/