研究论文 RESEARCH PAPER

猪妊娠期和分娩后肠道微生物多样性研究

  • 庞丽燕 ,
  • 丁鸿祥 ,
  • 肖霖力 ,
  • 刘彦 ,
  • 郑琛 ,
  • 冯涛
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  • 1. 甘肃农业大学动物科学技术学院, 兰州 730070;
    2. 北京市农林科学院畜牧兽医研究所, 北京 100097;
    3. 北京市农林科学院-美国俄克拉荷马州立大学动物科学联合实验室, 北京 100097
庞丽燕(1997-),女,甘肃陇南人,硕士研究生,研究方向为家畜繁殖的营养调控。E-mail:1554741308@qq.com

收稿日期: 2021-09-25

  网络出版日期: 2022-04-14

基金资助

国家自然科学基金项目(31972575);北京市自然科学基金项目(6202009)

Research on Gut Microbial Diversity of Sows during Pregnancy and after Parturition

  • PANG Liyan ,
  • DING Hongxiang ,
  • XIAO Linli ,
  • LIU Yan ,
  • ZHENG Chen ,
  • FENG Tao
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  • 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
    3. Joint Laboratory of Animal Science between Institute of Animal Husbandry and Veterinary Medicine of Beijing Academy of Agriculture and Forestry Sciences and Division of Agricultural Science and Natural Resource of Oklahoma State University, Beijing 100097, China

Received date: 2021-09-25

  Online published: 2022-04-14

摘要

本试验旨在研究母猪不同妊娠阶段肠道微生物区系的变化,分析肠道微生物与母体妊娠生理之间的关系,以期为维持正常妊娠提供参考。试验采用16S rRNA高通量测序技术,对长大二元健康母猪3个妊娠时期(妊娠30、70和110 d)和分娩后的肠道微生物组成进行研究。结果表明:随着妊娠日龄的增加,母猪肠道微生物多样性降低,且在妊娠30、70、110 d和分娩后肠道微生物的优势菌门均为厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)。在门水平上,随着妊娠的进行,螺旋体门(Spirochaetes)的相对丰度降低,变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、梭杆菌门(Fusobacteria)和Patescibacteria的相对丰度升高。在属水平上,随着妊娠的进行,普雷沃氏菌属(Prevotella)、卟啉单胞菌属(Porphyromonas)、梭杆菌属(Fusobacterium)、狭窄梭菌属1(Clostridium_sensu_stricto_1)、拟杆菌属(Bacteroides)、大肠埃希菌-志贺菌属(Escherichia-Shigella)的相对丰度升高,乳杆菌属(Lactobacillus)、密螺旋体属(Treponema)的相对丰度降低。其中,妊娠70和110 d时肠道菌群结构相似,且与妊娠30 d和分娩后肠道菌群结构差异较大,这可能与妊娠中后期母体代谢增强、体重增加、胎儿发育等相关。综上所述,母猪妊娠期肠道微生物是动态变化的,随着妊娠日龄的增加,肠道微生物多样性降低,丰富度升高,分娩后肠道微生物多样性和丰富度均降低;妊娠期和分娩后肠道中的优势菌门均为厚壁菌门和拟杆菌门,妊娠期的优势菌属为狭窄梭菌属1、乳杆菌属和拟杆菌属,分娩后的优势菌属为拟杆菌属、大肠埃希菌-志贺菌属和卟啉单胞菌属。

本文引用格式

庞丽燕 , 丁鸿祥 , 肖霖力 , 刘彦 , 郑琛 , 冯涛 . 猪妊娠期和分娩后肠道微生物多样性研究[J]. 动物营养学报, 2022 , 34(4) : 2208 -2220 . DOI: 10.3969/j.issn.1006-267x.2022.04.017

Abstract

This experiment was conducted to study the changes of gut microflora in sows at different stages during pregnancy and after delivery, and to analyze the relationship between intestinal microflora and maternal pregnancy physiology, in order to provide reference to maintain normal pregnancy. In this study, 16S rRNA high-throughput sequencing technology was used to reveal the gut microbial composition in 3 pregnancy stages (30, 70 and 110 d of pregnancy) and after delivery of healthy sows. The results showed that with the increase of gestational age, the gut microbial diversity of sows was decreased. The dominant phyla of gut microorganisms at 30, 70, 110 d of pregnancy and after delivery were Firmicutes and Bacteroidetes. At phylum level, the relative abundance of Spirochaetes was decreased with the progress of pregnancy, and the relative abundances of Proteobacteria, Actinobacteria and Fusobacteria and Patescibacteria were increased. At genus level, with the progress of pregnancy, the relative abundances of Prevotella, Porphyromona, Fusobacterium, Clostridium_sensu_stricto_1, Bacteroides and Escherichia-Shigella were increased, while the relative abundances of Lactobacillus and Treponema were decreased. The gut microbial community at 70 and 110 d of pregnancy was similar, but it was greatly different with that of 30 d of pregnancy and after delivery. All above indicated that microorganism development during pregnancy related with maternal metabolism enhancement, weight gain and fetal development in the middle and late pregnancy. In summary, the gut microflora of sows during pregnancy is dynamically changed. The diversity of gut microflora decreases and the abundance increases with the increase of gestational age, while the diversity and abundance decrease after delivery. The dominant bacterial phyla in the gut during pregnancy and after delivery are Firmicutes and Bacteroidetes, and the dominant bacterial genera are Clostridium_sensu_stricto_1, Lactobacillus and Bacteroides during pregnancy and Bacteroides, Escherichia-Shigella and Porphyromonas after delivery.

参考文献

[1] 翁善钢,陈琳,BRUTSAERT B,等.母猪肠道越健康,窝重越大[J].国外畜牧学(猪与禽),2015,35(3):21-22. WENG S G,CHEN L,BRUTSAERT B,et al.Healthy sow gut-higher litter weight[J].Animal Science Abroad (Pigs and Poultry),2015,35(3):21-22.(in Chinese)
[2] LEY R E,PETERSON D A,GORDON J I.Ecological and evolutionary forces shaping microbial diversity in the human intestine[J].Cell,2006,124(4):837-848.  
[3] CASTILLO M,MARTÍN-OR ÚE S M,ANGUITA M,et al.Adaptation of gut microbiota to corn physical structure and different types of dietary fibre[J].Livestock Science,2007,109(1/3):149-152.
[4] 杨伟平,王建刚,曹斌云.猪肠道微生物群落组成变化及其影响因素[J].中国畜牧杂志,2017,53(1):12-16. YANG W P,WANG J G,CAO B Y.Composition and influence factors of pigs’ gut microbial community[J].Chinese Journal of Animal Science,2017,53(1):12-16.(in Chinese)
[5] NURIEL-OHAYON M,NEUMAN H,KOREN O.Microbial changes during pregnancy,birth,and infancy[J].Frontiers in Microbiology,2016,7:1031.
[6] AL-ASMAKH M,HEDIN L,PETTERSSON S.Maternal microbiota regulate glucocorticoids levels and placental development in mice[J].Endocrine Abstracts,2015,37:OC5.1.
[7] VUONG H E,PRONOVOST G N,WILLIAMS D W,et al.The maternal microbiome modulates fetal neurodevelopment in mice[J].Nature,2020,586(7828):281-286.  
[8] 王津,马蕊,韩烨,等.母猪繁殖周期肠道微生物的研究[J].饲料研究,2016(20):30-34. WANG J,MA R,HAN Y,et al.Study on intestinal microflora of sows during reproductive cycle[J].Feed Research,2016(20):30-34.(in Chinese)
[9] LYNCH S V,PEDERSEN O.The human intestinal microbiome in health and disease[J].The New England Journal of Medicine,2016,375(24):2369-2379.  
[10] SCHOELER M,CAESAR R.Dietary lipids,gut microbiota and lipid metabolism[J].Reviews in Endocrine & Metabolic Disorders,2019,20(4):461-472.  
[11] SONNENBURG J L,BÄCKHED F.Diet-microbiota interactions as moderators of human metabolism[J].Nature,2016,535(7610):56-64.  
[12] 古金元,彭涛,胡东方,等.浅谈妊娠母猪生理特性及发酵饲料对其影响[J].猪业科学,2017,34(7):94-96. GU J Y,PENG T,HU D F,et al.Discussion on physiological characteristics of pregnant sows and effects of fermented feed on them[J].Swine Industry Science,2017,34(7):94-96.(in Chinese)
[13] HUANG X C,GAO J,ZHAO Y Z,et al.Dramatic remodeling of the gut microbiome around parturition and its relationship with host serum metabolic changes in sows[J].Frontiers in Microbiology,2019,10:2123.
[14] KOREN O,GOODRICH J K,CULLENDER T C,et al.Host remodeling of the gut microbiome and metabolic changes during pregnancy[J].Cell,2012,150(3):470-480.  
[15] KONG X F,JI Y J,LI H W,et al.Colonic luminal microbiota and bacterial metabolite composition in pregnant Huanjiang mini-pigs:effects of food composition at different times of pregnancy[J].Scientific Reports,2016,6:37224.
[16] LIU H B,HOU C L,LI N,et al.Microbial and metabolic alterations in gut microbiota of sows during pregnancy and lactation[J].The FASEB Journal,2019,33(3):4490-4501.  
[17] BISANZ J E,ENOS M K,PRAYGOD G,et al.Microbiota at multiple body sites during pregnancy in a rural Tanzanian population and effects of Moringa-supplemented probiotic yogurt[J].Applied and Environmental Microbiology,2015,81(15):4965-4975.  
[18] GOHIR W,WHELAN F J,SURETTE M G,et al.Pregnancy-related changes in the maternal gut microbiota are dependent upon the mother’s periconceptional diet[J].Gut Microbes,2015,6(5):310-320.  
[19] 李扬.纤维营养对妊娠母猪繁殖性能和肠道菌群的影响[D].博士学位论文.雅安:四川农业大学,2019. LI Y.Effects of dietary fiber in gestation diet on sow reproductive performance and gut microbiota[D].Ph.D.Thesis.Ya’an:Sichuan Agricultural University,2019.(in Chinese)
[20] 王仁杰,贺宇,张云常,等.妊娠后期与泌乳期母猪肠道微生物多样性研究[J].动物营养学报,2021,33(1):537-545. WANG R J,HE Y,ZHANG Y C,et al.A study on intestinal microbiota diversity of sows during late gestation and lactation periods[J].Chinese Journal of Animal Nutrition,2021,33(1):537-545.(in Chinese)
[21] CHENG C S,WEI H K,YU H C,et al.Metabolic syndrome during perinatal period in sows and the link with gut microbiota and metabolites[J].Frontiers in Microbiology,2018,9:1989.
[22] WANG H,JI Y C,YIN C,et al.Differential analysis of gut microbiota correlated with oxidative stress in sows with high or low litter performance during lactation[J].Frontiers in Microbiology,2018,9:1665.
[23] ZHAO J B,LIU P,HUANG C F,et al.Effect of wheat bran on apparent total tract digestibility,growth performance,fecal microbiota and their metabolites in growing pigs[J].Animal Feed Science and Technology,2018,239:14-26.
[24] 刘红宾.母猪微生物垂直传递影响仔猪肠道的微生物定植与功能发育[D].博士学位论文.北京:中国农业大学,2018. LIU H B.Vertical transmission of sows’ microbiota affects microbial colonization and functional development of intestine in piglets[D].Ph.D.Thesis.Beijing:China Agricultural University,2018.(in Chinese)
[25] 解培峰.妊娠环江香猪繁殖性能及其肠道微生物代谢特性研究[D].硕士学位论文.长沙:湖南农业大学,2018. XIE P F.Study on reproductive performance and intestinal microbial metabolic characteristics of pregnant Huanjiang mini-pigs[D].Master’s Thesis.Changsha:Hunan Agricultural University,2018.(in Chinese)
[26] 杨友成.关于猪机体对能量需要量的分析[J].饲料博览,2018(6):80. YANG Y C.Analysis of energy requirement of pig body[J].Feed Review,2018(6):80.(in Chinese)
[27] DAHIYA D K,RENUKA,PUNIYA M,et al.Gut microbiota modulation and its relationship with obesity using prebiotic fibers and probiotics:a review[J].Frontiers in Microbiology,2017,8:563.
[28] 刘壮.猪肠道微生物与机体营养代谢[J].饲料博览,2017(8):16-22. LIU Z.The pigs intestinal microbiota and host nutrient metabolism[J].Feed Review,2017(8):16-22.(in Chinese)
[29] FURUSAWA Y,OBATA Y,FUKUDA S,et al.Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells[J].Nature,2013,504(7480):446-450.  
[30] 田威龙,李月月,吕冬玲,等.猪不同生长阶段肠道微生物组的变化及其影响因素[J].中国畜牧兽医,2021,48(2):509-515. TIAN W L,LI Y Y,LV D L,et al.Changes of intestinal microbiome and its influencing factors at different growth stages in pigs[J].China Animal Husbandry & Veterinary Medicine,2021,48(2):509-515.(in Chinese)
[31] WLODARSKA M,WILLING B P,BRAVO D M,et al.Phytonutrient diet supplementation promotes beneficial Clostridia species and intestinal mucus secretion resulting in protection against enteric infection[J].Scientific Reports,2015,5:9253.
[32] ZHOU S H,GENG B,LI M J,et al.Comprehensive analysis of environmental factors mediated microbial community succession in nitrogen conversion and utilization of ex situ fermentation system[J].The Science of the Total Environment,2021,769:145219.
[33] LIU T,SUN L,MÜLLER B,et al.Importance of inoculum source and initial community structure for biogas production from agricultural substrates[J].Bioresource Technology,2017,245(Pt A):768-777.
[34] POWER S E,O’TOOLE P W,STANTON C,et al.Intestinal microbiota,diet and health[J].British Journal of Nutrition,2014,111(3):387-402.  
[35] 郭秀兰.猪肠道硬壁菌门和拟杆菌门数量的检测及其相对丰度与脂肪沉积的相关性研究[D].博士学位论文.雅安:四川农业大学,2008. GUO X L.Detection of Firmicutes and Bacteroidetes in the pig gut and the correlation between their abundance and fat deposit[D].Ph.D.Thesis.Ya’an:Sichuan Agricultural University,2008.(in Chinese)
[36] 陈宝剑,吴永绍,覃兆鲜,等.猪不同发育阶段肠道微生物菌群特征分析[J].中国畜牧杂志,2021,57(1):101-108. CHEN B J,WU Y S,QIN Z X,et al.Characteristics of intestinal microflora in different developmental stages of pigs[J].Chinese Journal of Animal Science,2021,57(1):101-108.(in Chinese)
[37] ZHANG J H,MENG H,KONG X C,et al.Combined effects of polyethylene and organic contaminant on zebrafish (Danio rerio):accumulation of 9-nitroanthracene,biomarkers and intestinal microbiota[J].Environmental Pollution,2021,277:116767.
[38] NEEDHAM B D,KADDURAH-DAOUK R,MAZMANIAN S K.Gut microbial molecules in behavioural and neurodegenerative conditions[J].Nature Reviews Neuroscience,2020,21(12):717-731.  
[39] 程雅婷,孔祥峰.短链脂肪酸的生理作用及其在母猪生产中的应用[J].动物营养学报,2021,33(10):5435-5440. CHENG Y T,KONG X F.Physiological functions of short-chain fatty acids and their application in sow production[J].Chinese Journal of Animal Nutrition,2021,33(10):5435-5440.(in Chinese)
[40] YU Y J,RAKA F,ADELI K.The role of the gut microbiota in lipid and lipoprotein metabolism[J].Journal of Clinical Medicine,2019,8(12):2227.
[41] 彭健.妊娠母猪背膘过厚影响繁殖性能的机制及其营养调控技术研究[J].饲料工业,2018,39(19):1-8. PENG J.Mechanism of declining reproductive performance by excessive backfat of sows during gestation and its nutritional regulation techniques[J].Feed Industry,2018,39(19):1-8.(in Chinese)
[42] HUANG G N,XU J,LEFEVER D E,et al.Genistein prevention of hyperglycemia and improvement of glucose tolerance in adult non-obese diabetic mice are associated with alterations of gut microbiome and immune homeostasis[J].Toxicology and Applied Pharmacology,2017,332:138-148.
[43] DI SIMONE N,SANTAMARIA ORTIZ A,SPECCHIA M,et al.Recent insights on the maternal microbiota:impact on pregnancy outcomes[J].Frontiers in Immunology,2020,11:528202.
[44] LIU Y,JIANG J,LEPIK B,et al.Subdomain 2,not the transmembrane domain,determines the dimerization partner of growth hormone receptor and prolactin receptor[J].Endocrinology,2017,158(10):3235-3248.  
[45] SAWYER G,WEBSTER D,NARAYAN E.Measuring wool cortisol and progesterone levels in breeding maiden Australian merino sheep (Ovis aries)[J].PLoS One,2019,14(4):e0214734.
[46] HUSSAIN T,MURTAZA G,KALHORO D H,et al.Relationship between gut microbiota and host-metabolism:emphasis on hormones related to reproductive function[J].Animal Nutrition,2021,7(1):1-10.  
[47] 薛云.孕酮和雌二醇对猪粪细菌区系的影响[D].硕士学位论文.南京:南京农业大学,2017. XUE Y.Effects of progesterone & estradiol on the fecal microflora in pigs[D].Master’s Thesis.Nanjing:Nanjing Agricultural University,2017.(in Chinese)
[48] SANTOS-MARCOS J A,RANGEL-ZUÑIGA O A,JIMENEZ-LUCENA R,et al.Influence of gender and menopausal status on gut microbiota[J].Maturitas,2018,116:43-53.
[49] SANTACRUZ A,COLLADO M C,GARCÍA-VALDÉS L,et al.Gut microbiota composition is associated with body weight,weight gain and biochemical parameters in pregnant women[J].British Journal of Nutrition,2010,104(1):83-92.  
[50] CANI P D,OSTO M,GEURTS L,et al.Involvement of gut microbiota in the development of low-grade inflammation and type 2 diabetes associated with obesity[J].Gut Microbes,2012,3(4):279-288.  
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