动物营养学报 >

2022 , Vol. 34 >Issue 4: 2272 - 2282

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.023

研究论文 RESEARCH PAPER

复合纤维和植物精油联合添加对大肠杆菌攻毒仔猪生长性能、抗氧化能力和免疫功能的影响

  • 梁婵 ,
  • 陈代文 ,
  • 何军 ,
  • 郑萍 ,
  • 毛湘冰 ,
  • 虞洁 ,
  • 罗玉衡 ,
  • 罗钧秋 ,
  • 黄志清 ,
  • 阎辉 ,
  • 余冰
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  • 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 成都 611130
梁婵(1993-),女,四川绵阳人,硕士研究生,从事猪营养研究。E-mail:liangc0903@163.com

收稿日期: 2021-08-26

  网络出版日期: 2022-04-14

基金资助

国家重点研发计划项目（2018YFD0500605）

收起

Effects of Compound Fiber and Plant Essential Oil Combined Supplementation on Growth Performance, Antioxidant Capacity and Immune Function of Weaned Piglets Challenged with Escherichia coli

  • LIANG Chan ,
  • CHEN Daiwen ,
  • HE Jun ,
  • ZHENG Ping ,
  • MAO Xiangbing ,
  • YU Jie ,
  • LUO Yuheng ,
  • LUO Junqiu ,
  • HUANG Zhiqing ,
  • YAN Hui ,
  • YU Bing
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  • Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2021-08-26

  Online published: 2022-04-14

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摘要

本试验旨在研究复合纤维和植物精油联合添加对产肠毒素大肠杆菌（ETEC）攻毒仔猪生长性能、抗氧化能力和免疫功能的影响。试验采用单因素设计，选取28头（28±1）日龄的健康“杜×长×大”断奶仔猪，根据体重[（7.93±0.44） kg]相近原则，随机分为4个组，分别为对照组（饲喂基础饲粮）、攻毒对照组（饲喂基础饲粮）、抗生素组（饲喂基础饲粮+75 mg/kg金霉素+25 mg/kg维吉尼亚霉素+50 mg/kg喹烯酮）和复合纤维+植物精油组（饲喂基础饲粮+0.5%菊粉+0.5%微晶纤维素+200 mg/kg植物精油），每组7个重复，每个重复1头仔猪，单笼饲养。试验期25 d。于试验第22天上午，对仔猪进行空腹称重后，各试验组试猪一次性灌服10 mL/kg BW大肠杆菌（6×109 CFU/mL）悬浮液，而对照组采用相同方式灌服生理盐水。结果表明:1）与对照组相比，攻毒对照组仔猪平均日增重（ADG）降低了7.19%（P>0.05），料重比（F/G）提高了5.59%（P>0.05），血清丙二醛（MDA）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）含量显著提高（P<0.05），血清免疫球蛋白A（IgA）和白细胞介素-4（IL-4）含量显著降低（P<0.05），空肠黏膜MDA含量以及白细胞介素-6（IL-6）、核转录因子-κB p65（NF-κB p65）、白细胞介素-1受体相关激酶1（IRAK1） mRNA相对表达量显著提高（P<0.05）。2）与攻毒对照组相比，复合纤维+植物精油组仔猪平均日采食量（ADFI）和ADG分别提高了26.06%和31.73%（P>0.05），血清总抗氧化能力（T-AOC）以及IgA和IL-4含量显著提高（P<0.05），血清和空肠黏膜MDA含量显著降低（P<0.05），空肠黏膜IL-6、Toll样受体4（TLR4）和NF-κB p65 mRNA相对表达量显著降低（P<0.05）。3）与抗生素组相比，复合纤维+植物精油组仔猪血清IgA含量显著提高（P<0.05），空肠黏膜IL-1β、TNF-α、CD14、髓样分化因子88（MyD88）、NF-κB p65和肿瘤坏死因子受体相关因子6（TRAF6） mRNA相对表达量显著降低（P<0.05）。综上所述，复合纤维和植物精油联合添加能显著抑制大肠杆菌诱导的仔猪氧化应激和炎症反应，改善仔猪生长性能，具有替代抗生素的潜力。

关键词： 复合纤维; 植物精油; ETEC; 仔猪; 生长性能; 抗氧化能力; 免疫功能

本文引用格式

梁婵 , 陈代文 , 何军 , 郑萍 , 毛湘冰 , 虞洁 , 罗玉衡 , 罗钧秋 , 黄志清 , 阎辉 , 余冰 . 复合纤维和植物精油联合添加对大肠杆菌攻毒仔猪生长性能、抗氧化能力和免疫功能的影响[J]. 动物营养学报, 2022 , 34(4) : 2272 -2282 . DOI: 10.3969/j.issn.1006-267x.2022.04.023

Abstract

This experiment was conducted to investigate the effects of compound fiber and plant essential oil combined supplementation on growth performance, antioxidant capacity and immune function of piglets challenged with enterotoxigenic Escherichia coli (ETEC). Twenty-eight healthy Duroc×Landrace×Yorkshire weaned piglets at (28±1) days of age were randomly divided into 4 groups according to the principle of similar body weight[(7.93±0.44) kg]. The 4 groups were control group (fed a basal diet), challenge control group (fed the basal diet), antibiotic group (fed the basal diet+75 mg/kg aureomycin+25 mg/kg virginiamycin+50 mg/kg quinocetone) and compound fiber and plant essential oil group (fed the basal diet+0.5% inulin+0.5% microcrystalline cellulose+200 mg/kg plant essential oil). There were 7 replicates in each group and 1 piglet in each replicate fed in a single cage. The experiment lasted for 25 days. On the morning of day 22, after fasting weighing, all pigs in the experimental groups were given 10 mL/kg BW Escherichia coli suspension (6×109 CFU/mL) at one time, while those in the control group were given normal saline in the same way. The results showed as follows:1) compared with the control group, the average daily gain (ADG) of piglets in the challenge control group was decreased by 7.19% (P>0.05), and the feed to gain ratio (F/G) was increased by 5.59% (P>0.05); the contents of malondialdehyde (MDA), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum were significantly increased (P<0.05); the contents of immunoglobulin A (IgA) and interleukin-4 (IL-4) in serum were significantly decreased (P<0.05); and the MDA content and mRNA relative expression levels of interleukin-6 (IL-6), nuclear factor-κB p65 (NF-κB p65) and interleukin-1 receptor-associated kinase 1 (IRAK1) in jejunal mucosa were significantly increased (P<0.05). 2) Compared with the challenge control group, the average daily feed intake (ADFI) and ADG of piglets in the compound fiber and plant essential oil group were increased by 26.06% and 31.73%, respectively (P>0.05); the total antioxidant capacity (T-AOC) and contents of IgA and IL-4 in serum were significantly increased (P<0.05); the MDA content in serum and jejunal mucosa was significantly decreased (P<0.05); and the mRNA relative expression levels of IL-6, Toll-like receptor 4 (TLR4) and NF-κB p65 in jejunal mucosa were significantly decreased (P<0.05). 3) Compared with the antibiotic group, the serum IgA content in the compound fiber and plant essential oil group was significantly increased (P<0.05); and the mRNA relative expression levels of IL-1β, TNF-α, CD14, myeloid differentiation factor 88 (MyD88), NF-κB p65 and tumor necrosis factor receptor-related factor 6 (TRAF6) in jejunal mucosa were significantly decreased (P<0.05). In conclusion, the compound fiber and plant essential oil combined supplementation can significantly inhibit the oxidative stress and inflammatory response induced by Escherichia coli in piglets, and improve the growth performance of piglets, which has the potential to replace antibiotics.

Key words： compound fiber; plant essential oil; ETEC; piglets; growth performance; antioxidant capacity; immune function

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