动物营养学报 >

2022 , Vol. 34 >Issue 4: 2283 - 2291

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.024

研究论文 RESEARCH PAPER

柑橘提取物对仔猪盲肠微生物数量和代谢产物的影响

  • 崔艺燕 ,
  • 鲁慧杰 ,
  • 李贞明 ,
  • 田志梅 ,
  • 余苗 ,
  • 邓盾 ,
  • 马现永
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  • 1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
    2. 岭南现代农业科学与技术广东省实验室茂名分中心, 茂名 525000
崔艺燕(1987-),女,广东佛山人,助理研究员,硕士,从事动物营养与饲料科学、生态养殖与环境控制研究。E-mail:958117076@qq.com

收稿日期: 2021-09-27

  网络出版日期: 2022-04-14

基金资助

省级农业科技创新推广及农业资源与生态环境保护建设项目（2021KJ266）；广东省农业科学院学科团队建设项目（202118TD）；饲料产业技术体系创新团队建设项目（2021KJ115）；科技创新战略专项资金（高水平农科院建设）——杰出人才（R2020PY-JC001）和优秀博士（R2020YJ-YB2002）；韶关市科技计划项目（200731114530834）

收起

Effects of Citrus Extract on Number of Microbes and Metabolites in Cecum of Piglets

  • CUI Yiyan ,
  • LU Huijie ,
  • LI Zhenming ,
  • TIAN Zhimei ,
  • YU Miao ,
  • DENG Dun ,
  • MA Xianyong
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  • 1. Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China

Received date: 2021-09-27

  Online published: 2022-04-14

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摘要

本试验旨在研究柑橘提取物对仔猪盲肠微生物数量和代谢产物的影响。选取144头28日龄的杜×长×大仔猪[体重（8.39±1.06） kg]，随机分为3组，每组6个重复，每个重复8头猪（公母各占1/2）。对照组饲喂基础饲粮，抗生素组在基础饲粮中添加75 g/t金霉素，柑橘提取物组在基础饲粮中添加300 mL/t柑橘提取物。试验期28 d。结果表明:1）与对照组相比，柑橘提取物组盲肠内容物中乳酸菌数量显著增加（P<0.05），抗生素组乳酸菌数量显著减少（P<0.05）；与对照组相比，柑橘提取物组和抗生素组盲肠大肠杆菌数量显著减少（P<0.05），柑橘提取物组盲肠大肠杆菌数量显著低于抗生素组（P<0.05）。2）与对照组和抗生素组相比，柑橘提取物组盲肠内容物中牛磺酸、苏氨酸、丝氨酸、缬氨酸、亮氨酸、赖氨酸的含量显著减少（P<0.05）；与对照组相比，饲粮添加柑橘提取物显著减少盲肠内容物中天冬氨酸、异亮氨酸、胱硫醚、羟赖氨酸、鸟氨酸、脯氨酸、总氨基酸含量（P<0.05），有减少谷氨酸和丙氨酸含量的趋势（0.05 ≤ P<0.10），而抗生素显著减少鸟氨酸和脯氨酸含量（P<0.05）。3）与对照组相比，柑橘提取物组盲肠内容物中苯酚和粪臭素含量显著减少（P<0.05）；与对照组和抗生素组相比，柑橘提取物组盲肠内容物中腐胺含量显著减少（P<0.05），酪胺和精胺含量有减少的趋势（0.05 ≤ P<0.10）；与对照组相比，柑橘提取物组和抗生素组盲肠内容物的甲胺含量显著减少（P<0.05）。由此可见，柑橘提取物增加了仔猪盲肠内容物中乳酸菌数量，减少了代谢产物的含量和大肠杆菌数量。

关键词： 柑橘提取物; 仔猪; 生物胺; 游离氨基酸; 代谢产物

本文引用格式

崔艺燕 , 鲁慧杰 , 李贞明 , 田志梅 , 余苗 , 邓盾 , 马现永 . 柑橘提取物对仔猪盲肠微生物数量和代谢产物的影响[J]. 动物营养学报, 2022 , 34(4) : 2283 -2291 . DOI: 10.3969/j.issn.1006-267x.2022.04.024

Abstract

This experiment aimed to investigate the effects of citrus extract on the number of microbes and metabolites in the cecal content of piglets. One hundred and forty-four 28-day-old Duroc×Landrace×Large White pigs with body weight of (8.39±1.06) kg were randomly assigned to 3 groups with 6 replicates per group and 8 pigs per pen (4 boars, 4 females). Piglets in the control group were fed a basal diet, piglets in the antibiotic group were fed the basal diet supplemented with 75 g/t chlortetracycline, and piglets in the citrus extract group were fed the basal diet supplemented with 300 mL/t citrus extract. The experiment lasted for 28 d. The results showed as follows:1) compared with control group, dietary citrus extract significantly increased the number of lactic acid bacteria in cecal content of piglets (P<0.05), while antibiotic significantly decreased the number of lactic acid bacteria (P<0.05). Compared with control group, dietary citrus extract and antibiotic significantly decreased the number of Escherichia coli in cecal content of piglets (P<0.05), and the number of Escherichia coli in citrus extract group was significantly lower than that in antibiotic group (P<0.05). 2) Compared with control and antibiotic groups, dietary citrus extract significantly increased the contents of taurine, threonine, serine, valine, leucine and lysine in cecal content of piglets (P<0.05). Compared with control group, dietary citrus extract significantly decreased the contents of aspartic acid, isoleucine, cystathionine, hydroxylysine, ornithine, proline and total amino acids in cecal content of piglets (P<0.05) and tended to decrease the contents of glutamate and alanine (0.05 ≤ P<0.10), and dietary antibiotic significantly decreased the content of ornithine and proline (P<0.05). 3) Compared with control group, dietary citrus extract significantly decreased the contents of phenol and skatole (P<0.05). Compared with control and antibiotic groups, dietary citrus extract significantly decreased the content of putrescine (P<0.05) and tended to decrease the contents of tyramine and spermine in cecal content of piglets (0.05 ≤ P<0.10). Compared with control group, dietary citrus extract and antibiotic significantly decreased the contents of methylamine in cecal content of piglets (P<0.05). In conclusion, citrus extract increases the number of lactic acid bacteria in cecal content of piglets, and decreases the contents of metabolites and the number of Escherichia coli.

Key words： citrus extract; piglets; biogenic amine; free amino acid; metabolite

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