研究论文 RESEARCH PAPER

饲粮添加不同水平维生素E对29~63日龄江南白鹅生长性能、免疫性能和抗氧化性能的影响

  • 孙庆雨 ,
  • 杨海明 ,
  • 徐学安 ,
  • 郁钧 ,
  • 陈媛婧 ,
  • 王志跃
展开
  • 1. 扬州大学动物科学与技术学院, 扬州 225009;
    2. 扬州大学农业科技发展研究院, 扬州 225009
孙庆雨(1996-),男,安徽宿州人,硕士研究生,从事家禽营养与生产研究。E-mail:2546774743@qq.com

收稿日期: 2021-09-18

  网络出版日期: 2022-04-14

基金资助

财政部和农业农村部国家现代农业产业技术体系;江苏省农业重大新品种创制项目(PZCZ201738);江苏省现代农业(水禽)产业技术体系建设专项(JATS[2021]496)

Effects of Dietary Vitamin E Supplemental Level on Growth Performance, Immune Capacity and Antioxidant Capacity of Jiangnan White Goslings from 29 to 63 Days of Age

  • SUN Qingyu ,
  • YANG Haiming ,
  • XU Xuean ,
  • YU Jun ,
  • CHEN Yuanjing ,
  • WANG Zhiyue
Expand
  • 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China

Received date: 2021-09-18

  Online published: 2022-04-14

摘要

本试验旨在研究饲粮添加不同水平维生素E对29~63日龄江南白鹅生长性能、免疫性能和抗氧化性能的影响,以确定29~63日龄江南白鹅饲粮中适宜的维生素E添加水平。选取同一批出雏、健康且体重相近的28日龄江南白鹅公鹅240只,随机分为6组,每组5个重复,每个重复8只,分别在基础饲粮中添加0、5、10、20、40和80 mg/kg维生素E,试验期为35 d。结果表明:1)饲粮添加不同水平维生素E对29~63日龄仔鹅的体重、平均日采食量、平均日增重和料重比无显著影响(P>0.05)。2)饲粮添加维生素E显著降低仔鹅血清皮质醇的含量(P<0.05);以血清中皮质醇的含量为效应指标,通过折线模型估测29~63日龄江南白鹅饲粮中适宜的维生素E添加水平为19.33 mg/kg。3)与对照组相比,饲粮添加20 mg/kg维生素E显著提高仔鹅血清免疫球蛋白A含量(P<0.05);添加20、40 mg/kg维生素E显著提高仔鹅血清中免疫球蛋白M含量(P<0.05)。4)饲粮添加维生素E显著提高血清和肝脏中过氧化氢酶、谷胱甘肽过氧化物酶的活性、总抗氧化能力以及降低血清和肝脏中丙二醛的含量(P<0.05);以血清和肝脏中丙二醛的含量为效应指标,通过折线模型估测29~63日龄江南白鹅饲粮中适宜的维生素E添加水平分别为12.37、21.73 mg/kg。综上所述,29~63日龄江南白鹅饲粮中维生素E适宜添加水平为20 mg/kg。

本文引用格式

孙庆雨 , 杨海明 , 徐学安 , 郁钧 , 陈媛婧 , 王志跃 . 饲粮添加不同水平维生素E对29~63日龄江南白鹅生长性能、免疫性能和抗氧化性能的影响[J]. 动物营养学报, 2022 , 34(4) : 2425 -2434 . DOI: 10.3969/j.issn.1006-267x.2022.04.037

Abstract

The purpose of this experiment was to find out the optimal vitamin E supplemental level in the diet of Jiangnan white goslings from 29 to 63 days of age by investigating the effects of different levels of vitamin E on the growth performance, immune capacity and antioxidant capacity of Jiangnan white goslings. A total of 240 healthy 28-day-old Jiangnan white goslings were selected and randomly divided into 6 groups with 5 replicates in each group and 8 goslings in each replicate. Based on the basis of corn-soybean meal diet, the vitamin E supplemental levels of groups were 0, 5, 10, 20, 40 and 80 mg/kg, respectively. The experimental period was 35 days. The results showed as follows:1) dietary vitamin E supplemental level had no significant effect on the body weight, average daily feed intake, average daily weight gain and feed to gain ratio of goslings from 29 to 63 days of age (P>0.05). 2) Vitamin E supplementation in diet could significantly reduce the content of cortisol in serum of goslings (P<0.05); based on the content of cortisol in serum, the optimal dietary vitamin E supplemental level estimated by broken line regression model for Jiangnan white goslings from 29 to 63 days of age was 19.33 mg/kg. 3) Compared with the control group, the diet supplemented 20 mg/kg vitamin E could significantly increase the content of immunoglobulin A in serum of goslings (P<0.05). The diet supplemented 20 and 40 mg/kg could significantly increase the content of immunoglobulin M in serum of goslings (P<0.05). 4) Vitamin E supplementation in diet could significantly increase the activity of catalase, glutathione peroxidase, total antioxidant capacity and reduce the content of malondialdehyde in serum and liver of goslings (P<0.05). Based on the content of malondialdehyde in serum and liver, the optimal dietary vitamin E supplemental levels estimated by broken line regression model for Jiangnan white gosling from 29 to 63 days of age are 12.37 and 21.73 mg/kg, respectively. In summary, the optimal dietary vitamin E supplemental level is 20 mg/kg.

参考文献

[1] 马田,邓子新,刘天罡.维生素E的"前世"和"今生"[J].合成生物学,2020,1(2):174-186. MA T,DENG Z X,LIU T G.The past and present of vitamin E[J].Synthetic Biology Journal,2020,1(2):174-186.(in Chinese)
[2] GAO J,LIN H,WANG X J,et al.Vitamin E supplementation alleviates the oxidative stress induced by dexamethasone treatment and improves meat quality in broiler chickens[J].Poultry Science,2010,89(2):318-327.  
[3] SELIM N A,YOUSSEF S F,ABDEL-SALAM A F,et al.Evaluation of some natural antioxidant sources in broiler diets:1-effect on growth,physiological,microbiological and immunological performance of broiler chicks[J].International Journal of Poultry Science,2013,12(10):561-571.  
[4] TRUSHENSKI J T,KOHLER C C.Influence of stress and dietary natural-source vitamin E on nonspecific immunocompetence,tissue tocopherol composition,and postslaughter fillet oxidative stability in sunshine bass[J].North American Journal of Aquaculture,2007,69(4):330-339.  
[5] CALFEE-MASON K G,SPEAR B T,GLAUERT H P.Effects of vitamin E on the NF-kappaB pathway in rats treated with the peroxisome proliferator,ciprofibrate[J].Toxicology and Applied Pharmacology,2004,199(1):1-9.  
[6] RIZVI S,RAZA S T,AHMED F,et al.The role of vitamin E in human health and some diseases[J].Sultan Qaboos University Medical Journal,2014,14(2):e157-e165.
[7] LIU Y J,ZHAO L H,MOSENTHIN R,et al.Protective effect of vitamin E on laying performance,antioxidant capacity,and immunity in laying hens challenged with Salmonella enteritidis[J].Poultry Science,2019,98(11):5847-5854.  
[8] 黄路生.维生素E对蛋种鸭繁殖性能及免疫功能影响的研究[D].硕士学位论文.广州:华南农业大学,2017. HUANG L S.Effects of vitamin E on reproductive performance and immune functions in laying duck breeders[D].Master’s Thesis.Guangzhou:South China Agricultural University,2017.(in Chinese)
[9] 王思维.维生素E对蛋雏鸭生长性能及血液生化指标的影响[D].硕士学位论文.哈尔滨:东北农业大学,2013. WANG S W.Effects of vitamin E on growth performance and biochemical indexes of egg-type ducklings[D].Master’s Thesis.Harbin:Northeast Agricultural University,2013.(in Chinese)
[10] 范文娜,何瑞国.维生素E对溆浦鹅肥肝抗氧化性能和血液生化指标影响的研究[J].中国粮油学报,2008,23(3):124-130. FAN W N,HE R G.Effects of VE supplement on antioxidation character of fatty liver and blood biochemical indices of geese[J].Journal of the Chinese Cereals and Oils Association,2008,23(3):124-130.(in Chinese)
[11] ROBBINS K R,SAXTON A M,SOUTHERN L L.Estimation of nutrient requirements using broken-line regression analysis[J].Journal of Animal Science,2006,84(Suppl.13):E155-E165.
[12] ISMAIL F S A,EL-GOGARY M R,EL-NADI M I.Influence of vitamin E supplementation and stocking density on performance,thyroid status,some blood parameters,immunity and antioxidant status in broiler chickens[J].Asian Journal of Animal and Veterinary Advances,2014,9(11):702-712.  
[13] ZHANG X,WANG G,ZHOU Y,et al.Effect of RRR-α-tocopherol succinate on the meat quality and antioxidative status in broilers[J].South African Journal of Animal Science,2011,42(4):341-353.
[14] XIE M,WANG S,HWANG W,et al.Effects of vitamin E on growth performance,tissue α-tocopherol,and lipid peroxidation of starter White Pekin ducks[J].Poultry Science,2018,97:2139-2143.
[15] POMPEU M A,CAVALCANTI L F L,TORAL F L B.Effect of vitamin E supplementation on growth performance,meat quality,and immune response of male broiler chickens:a Meta-analysis[J].Livestock Science,2018,208:5-13.
[16] MIN Y N,LIU S G,QU Z X,et al.Effects of dietary threonine levels on growth performance,serum biochemical indexes,antioxidant capacities,and gut morphology in broiler chickens[J].Poultry Science,2017,96(5):1290-1297.  
[17] JOSHP P K,BOSE M,HARISH D.Changes in certain haematological parameters in a siluroid cat fish Clarias batrachus (Linn) exposed to cadmium chloride[J].Pollution Research,2002,21(2):129-131.
[18] El-DEMERDASH F M.Antioxidant effect of vitamin E and selenium on lipid peroxidation,enzyme activities and biochemical parameters in rats exposed to aluminium[J].Journal of Trace Elements in Medicine and Biology,2005,18(1):113-121.
[19] SAHIN K,KUCUK O,SAHIN N,et al.Effects of vitamin C and vitamin E on lipid peroxidation status, serum hormone,metabolite,and mineral concentrations of Japanese quails reared under heat stress (34 degrees C)[J].International Journal for Vitamin and Nutrition Research,2002,72(2):91-100.  
[20] 胡志萍,寇涛,张莉莉,等.不同剂型维生素E对肉鸡生长性能、血清生化和抗氧化功能的影响[J].中国粮油学报,2016,31(4):118-123. HU Z P,KOU T,ZHANG L L,et al.Effects of different types of VE on growth performance,serum biochemical parameters and antioxidant capacity in broilers[J].Journal of the Chinese Cereals and Oils Association,2016,31(4):118-123.(in Chinese)
[21] 武江利,任延铭,王安,等.维生素E添加水平对笼养生长蛋鸭血清生化指标的影响[J].东北农业大学学报,2009,40(2):70-74. WU J L,REN Y M,WANG A,et al.Effects of vitamin E on serum biochemical indexes in growing ducks in cages[J].Journal of Northeast Agricultural University,2009,40(2):70-74.(in Chinese)
[22] 詹腾飞,韩云胜,汤超华,等.富维生素E转基因玉米对肉鸡生长性能、生化指标及抗氧化功能的影响[J].中国畜牧兽医,2019,46(9):2608-2617. ZHAN T F,HAN Y S,TANG C H,et al.Effects of vitamin E-riched transgenic maize on growth performance,biochemical parameters and antioxidant capacity of broilers[J].China Animal Husbandry & Veterinary Medicine,2019,46(9):2608-2617.(in Chinese)
[23] 赵丹阳,吴雨珊,李军国,等.饲粮添加亚麻籽和维生素E对北京油鸡肌肉n-3多不饱和脂肪酸富集和抗氧化特性的影响[J].动物营养学报,2020,32(11):5243-5254. ZHAO D Y,WU Y S,LI J G,et al.Effects of adding flaxseed and vitamin E in diets on n-3 polyunsaturated fatty acids enrichment and antioxidant properties of muscle in Beijing fatty chicken[J].Chinese Journal of Animal Nutrition,2020,32(11):5243-5254.(in Chinese)
[24] YARDIMCI M,SAHIN E H,CETINGUL I S,et al.Stress responses to comparative handling procedures in sheep[J].Animal,2013,7(1):143-150.  
[25] 王改琴.RRR-α-生育酚琥珀酸酯对肉鸡生产性能、抗氧化及免疫功能的影响[D].硕士毕业论文.南京:南京农业大学,2010. WANG G Q.Effects of RRR-a-tocopherol succinate on growth performance,antioxidant and immune function of broilers[D].Master’s Thesis.Nangjing:Nanjing Agricultural University,2010.(in Chinese)
[26] HASSOUN E A,STOHD S J.Comparative studies on oxidative stress as a mechanism for the fetotoxicity of TCDD,endrin and lindane in C57BL/6J and DBA/2J mice[J].Teratology,1995,51(3):186.
[27] LESHCHINSKY T V,KLASING K C.Profile of chicken cytokines induced by lipopolysaccharide is modulated by dietary alpha-tocopheryl acetate[J].Poultry Science,2003,82(8):1266-1273.  
[28] LI-WEBER M,GIAISI M,TREIBER M K,et al.Vitamin E inhibits IL-4 gene expression in peripheral blood T cells[J].European Journal of Immunology,2002,32(9):2401-2408.  
[29] LIU X,BYRD J A,FARNELL M,et al.Arginine and vitamin E improve the immune response after a Salmonella challenge in broiler chicks[J].Poultry Science,2014,93(4):882-890.  
[30] LIU Y J,ZHAO L H,MOSENTHIN R,et al.Protective effect of vitamin E on laying performance,antioxidant capacity,and immunity in laying hens challenged with Salmonella enteritidis[J].Poultry Science,2019,98:5847-5854
[31] NIU Z Y,LIU F Z,YAN Q L,et al.Effects of different levels of vitamin E on growth performance and immune responses of broilers under heat stress[J].Poultry Science,2009,88(10):2101-2107.  
[32] ZHANG X H,ZHONG X,ZHOU Y M,et al.Dietary RRR-α-tocopherol succinate attenuates lipopolysaccharide-induced inflammatory cytokines secretion in broiler chicks[J].British Journal of Nutrition,2010,104(12):1796-1805.  
[33] SINGH U,DEVARAJ S,JIALAL I.Vitamin E,oxidative stress,and inflammation[J].Annual Review of Nutrition,2005,25:151-174.
[34] WU D Y,MEYDANI S N.Age-associated changes in immune and inflammatory responses:impact of vitamin E intervention[J].Journal of Leukocyte Biology,2008,84(4):900-914.  
[35] SURAI P F,KOCHISH I I,ROMANOV M N,et al.Nutritional modulation of the antioxidant capacities in poultry:the case of vitamin E[J].Poultry Science,2019,98(9):4030-4041.  
[36] SORDILLO L M,CONTRERAS G A,AITKEN S L.Metabolic factors affecting the inflammatory response of periparturient dairy cows[J].Animal Health Research Reviews,2009,10(1):53-63.  
[37] NIU Z Y,MIN Y N,LIU F Z.Dietary vitamin E improves meat quality and antioxidant capacity in broilers by upregulating the expression of antioxidant enzyme genes[J].Journal of Applied Animal Research,2018,46(1):397-401.  
[38] 詹腾飞.富维生素E转基因玉米对肉鸡生产性能、免疫和抗氧化功能的影响[D].硕士毕业论文.北京:中国农业科学院,2019. ZHAN T F.Effects of vitamin E-riched transgenic maize on production performance, immune and antioxidant function of broilers[D].Master’s Thesis.Beijing:Chinese Academy of Agricultural Sciences,2019.(in Chinese)
文章导航

/