研究论文 RESEARCH PAPER

聚乙二醇甘油蓖麻酸酯对奶牛瘤胃发酵和微生物区系的影响

  • 郭晓辉 ,
  • 郑会超 ,
  • 王亚琴 ,
  • 苑志朋 ,
  • 黄国锋 ,
  • 蒋永清
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  • 1. 浙江省农业科学院畜牧兽医研究所, 杭州 310021;
    2. 浙江师范大学化学与生命科学学院, 金华 321004;
    3. 浙江省宁波市农机畜牧中心, 宁波 315000;
    4. 浙江省余姚市陆埠镇农业农村办, 余姚 315402
郭晓辉(1994-),男,河南周口人,硕士研究生,研究方向为反刍动物营养与饲养。E-mail:272448345@qq.com

收稿日期: 2021-09-27

  网络出版日期: 2022-04-14

基金资助

现代农业产业技术体系建设专项资金(CARS-36);浙江省重大科技专项重大农业项目(2016C02054-8)

Effects of Polyethylene Glycol Glycerol Castor Oil Ester on Rumen Fermentation and Microflora of Dairy Cows

  • GUO Xiaohui ,
  • ZHENG Huichao ,
  • WANG Yaqin ,
  • YUAN Zhipeng ,
  • HUANG Guofeng ,
  • JIANG Yongqing
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  • 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
    3. Ningbo Agricultural Machinery and Animal Husbandry Center, Ningbo 315000, China;
    4. Agricultural and Rural Affairs Office of Lubu Town, Yuyao 315402, China

Received date: 2021-09-27

  Online published: 2022-04-14

摘要

本试验旨在研究乳化剂聚乙二醇甘油蓖麻酸酯(PEGA)对荷斯坦奶牛瘤胃发酵和微生物区系的影响。利用3头年龄5.5岁左右、体重相近、处于泌乳末期的安装永久性瘤胃瘘管的健康荷斯坦奶牛作为试验动物,采用3×3拉丁方试验设计,对照组(Ⅰ组)饲喂基础饲粮和羊草,试验组(Ⅱ组和Ⅲ组)在基础饲粮中每头牛每天分别添加25和50 g PEGA。每日早、晚2次人工添加PEGA到基础饲粮中,搅拌均匀。每期试验15 d,预试期12 d,正试期3 d。结果表明:与Ⅰ组相比,饲粮中添加PEGA未显著影响瘤胃液pH和氨态氮(NH3-N)浓度(P>0.05),可显著降低异丁酸比例(P<0.05);对奶牛瘤胃中α-淀粉酶(α-AMS)、脂肪酶(LPS)、纤维素酶(CL)和胃蛋白酶(PP)活性无显著影响(P>0.05);显著增加普雷沃氏菌科UCG-001(Prevotellaceae_UCG-001)相对丰度(P<0.05);饲粮中添加25 g PEGA可显著提高奶牛瘤胃液戊酸比例(P<0.05),显著降低瘤胃克里斯滕森菌科R7群(Christensenellaceae_R-7_group)、理研菌科RC9肠道群(Rikenellaceaee_RC9_gut_group)和未鉴定菌属(unidentified)相对丰度(P<0.05)。综上所述,饲粮中添加PEGA可微调瘤胃微生物区系和挥发性脂肪酸组成,对奶牛常规瘤胃发酵参数没有显著影响。

本文引用格式

郭晓辉 , 郑会超 , 王亚琴 , 苑志朋 , 黄国锋 , 蒋永清 . 聚乙二醇甘油蓖麻酸酯对奶牛瘤胃发酵和微生物区系的影响[J]. 动物营养学报, 2022 , 34(4) : 2435 -2445 . DOI: 10.3969/j.issn.1006-267x.2022.04.038

Abstract

This experiment was conducted to study the effects of polyethylene glycol glycerol castor oil ester (PEGA) on rumen fermentation and microflora of Holstein dairy cows. Three healthy Holstein dairy cows equipped with permanent rumen fistulas were used in a 3×3 Latin square design. The cows were 5.5 years old, at the end of lactation, with similar body weight. The cows in the control group (group Ⅰ) were fed only a basal diet and leymus chinensis, and the cows in the experimental groups (groups Ⅱ and Ⅲ) were supplemented with 25 and 50 g emulsifier PEGA per day, respectively. The PEGA was artificially added to the basal diet twice a day and stirred well. Each trial period lasted for 15 days, with 12 days for adaptation and 3 days for sampling. Results showed that compared with group Ⅰ, dietary supplement PEGA had no effect on rumen fluid pH and ammoniacal nitrogen (NH3-N) concentration (P>0.05), but significantly decreased the proportion of isobutyric acid (P<0.05); the activities of α-amylase, lipase, cellulase and pepsin in rumen of dairy cows were not significantly influenced (P>0.05); PEGA could increase the relative abundance of Prevotellaceae_UCG-001 genus (P<0.05); diet supplement 25 g PEGA could increase proportion of valerate and decrease the relative abundance of Christensenellaceae_R-7_group, Rikenellaceaee_RC9_gut_group and unidentified bacteria (P<0.05). Above all, PEGA can modulate rumen microbial composition and volatile fatty acids pattern without routine fermentation parameters change.

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