研究论文 RESEARCH PAPER

甘露寡糖对饲喂荞麦秸秆饲粮滩羊瘤胃真菌菌群的影响

  • 马秀花 ,
  • 温红瑞 ,
  • 马步仓 ,
  • 杨万宗 ,
  • 周玉香
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  • 宁夏大学农学院, 银川 750021
马秀花(1995-),女,回族,宁夏吴忠人,硕士研究生,研究方向为反刍动物营养。E-mail:2095602677@qq.com

收稿日期: 2021-09-16

  网络出版日期: 2022-04-14

基金资助

农业部公益性行业(农业)项目(201503134)

Effects of Mannose-Oligosaccharides on Rumen Fungal Flora of Tan Sheep Fed Buckwheat Straw Diet

  • MA Xiuhua ,
  • WEN Hongrui ,
  • MA Bucang ,
  • YANG Wanzong ,
  • ZHOU Yuxiang
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  • College of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2021-09-16

  Online published: 2022-04-14

摘要

本试验是在荞麦秸秆饲粮条件下利用内部转录间隔区(ITS)测序技术探究添加1%、2%、3%甘露寡糖(MOS)对滩羊瘤胃真菌菌群的影响。选择健康无病、30.5 kg左右的宁夏断奶滩羊羯羊20只,随机分为4组,每组5只。CK-3组(对照组)饲喂基础饲粮,Y1-3组基础饲粮与1%MOS混合饲喂,Y2-3组基础饲粮与2%MOS混合饲喂,Y3-3组基础饲粮与3%MOS混合饲喂,预试期15 d,正试期60 d。结果显示:1)Y1-3组、Y2-3组、Y3-3组的滩羊瘤胃真菌菌群丰度及多样性较CK-3组明显提高,其中以Y3-3组效果最明显。2)在门水平上,子囊菌门(Ascomycota)、黄藻门(Anthophyta)、担子菌门(Basidiomycota)为4组中共有的优势菌门,但它们的相对丰度在4组间差异不显著(P>0.05)。在属水平上,Y3-3组曲霉属(Aspergillus)的相对丰度显著高于其他3组(P<0.05)。Y1-3组病原型营养型、腐生-病原-共生营养型、共生营养型的丰度相对其他组最少。总体而言,本试验条件下,荞麦秸秆饲粮中添加3%MOS更有利于增加滩羊瘤胃真菌菌群的多样性,而添加1%MOS时滩羊瘤胃真菌菌群可能更有利于纤维的降解与瘤胃健康的维系。

本文引用格式

马秀花 , 温红瑞 , 马步仓 , 杨万宗 , 周玉香 . 甘露寡糖对饲喂荞麦秸秆饲粮滩羊瘤胃真菌菌群的影响[J]. 动物营养学报, 2022 , 34(4) : 2514 -2523 . DOI: 10.3969/j.issn.1006-267x.2022.04.046

Abstract

In this experiment, internal transcribed spacer (ITS) sequencing technology was used to investigate the effects of adding 1%, 2% and 3% mannose oligosaccharides (MOS) on the rumen fungal flora of Tan sheep under the condition of buckwheat straw diet. Twenty healthy and disease-free weaned Ningxia Tan wethers with the body weight about 30.5 kg were randomly divided into 4 groups with 5 sheep in each group. Sheep in CK-3 group (control group) were fed a basal diet, sheep in Y1-3 group were fed the basal diet mixed with 1% MOS feeding, sheep in Y2-3 group were fed the basal diet mixed with 2% MOS, and sheep in Y3-3 group were fed the basal diet mixed with 3% MOS. The pre-feeding period was 15 days, and the normal feeding period was 60 days. The results showed as follows:1) the abundance and diversity of rumen fungal flora of Tan sheep in the Y1-3, Y2-3 and Y3-3 groups were obviously enhanced, and the effect of Y3-3 group was the most obvious. 2) At the phylum level, Ascomycota, Anthophyta and Basidiomycota were the dominant bacteria phyla shared by the four groups, but the differences in the relative abundances of them among the four groups were not significant (P>0.05). At the genus level, the relative abundance of Aspergillus in the Y-3 group was significantly higher than that in the other three groups (P<0.05). The abundance of disease prototype trophic type, saprophytic-pathogen-symbiotic trophic type, and symbiotic trophic type in the Y1-3 group was the least relative to other groups. In general, the addition of 3% MOS in the buckwheat straw diet under the experimental conditions is more conducive to increasing the diversity of rumen fungal flora of Tan sheep than other groups, and the rumen fungal flora of Tan sheep when diet supplemented with 1% MOS is more conducive to fiber degradation and rumen health.

参考文献

[1] 彭婉婉.日粮中添加葡萄籽对多浪羊营养物质消化代谢及瘤胃菌群的影响[D].硕士学位论文.阿拉尔:塔里木大学,2020. PENG W W.Digestion and metabolism of nutrients in Duolang sheep by adding grape seed in the diet and the influence of rumen flora[D].Master’s Thesis.Alaer:Tarim University,2020.(in Chinese)
[2] WILSON C A,WOOD T M.Studies on the cellulase of the rumen anaerobic fungus Neocallimastix frontalis,with special reference to the capacity of the enzyme to degrade crystalline cellulose[J].Enzyme and Microbial Teehnology,1992,14(4):258-264.  
[3] 高爱武.分别驱除厌氧真菌和原虫对绵羊瘤胃微生物种群及纤维物质降解的影响[D].博士学位论文.呼和浩特:内蒙古农业大学,2008. GAO A W.Effects of eliminating anaerobic fungi and defaunation respectively on ruminal microbes and fiber digestibility in sheep[D].Ph.D.Thesis.Hohhot:Inner Mongolia Agricultural University,2008.(in Chinese)
[4] 冯薇,王加启,刘开朗,等.参与瘤胃内纤维素降解过程的主要微生物研究进展[J].中国畜牧兽医,2011,38(2):37-42. FENG W,WANG J Q,LIU K L,et al.The research advance of the processes of cellulose degradation in rumen and related microoganisms[J].China Animal Husbandry & Veterinary Medicine,2011,38(2):37-42.(in Chinese)
[5] 马秀花,桂瑞麒,焦娜,等.荞麦秸秆饲粮条件下甘露寡糖对滩羊瘤胃菌群结构的影响[J].动物营养学报,2021,33(4):2365-2377. MA X H,GUI R Q,JIAO N,et al.Effect of mannan oligosaccharides on rumen bacterial flora structure of Tan sheep under buckwheat straw diet condition[J].Chinese Journal of Animal Nutrition,2021,33(4):2365-2377.(in Chinese)
[6] 张辉.瘤胃内真菌降解粗纤维的研究进展[J].黄牛杂志,1997(2):49-51. ZHANG H.Research progress on the degradation of crude fiber by fungi in the rumen[J].Journal of Yellow Cattle Science,1997(2):49-51.(in Chinese)
[7] LEE S S,SHIN K J,KIM W Y,et al.The rumen ecosystem:as a fountain sources of nobel enzymes—review[J].Animal Bioscience,1999,12(6):988-1001.
[8] 王海英,郭守玉,黄满荣,等.子囊菌较担子菌具有更快的进化速率和更高的物种多样性[J].中国科学(生命科学),2010,40(8):731-737,765-772. WANG H Y,GUO S Y,HUANG M R,et al.Ascomycetes have a faster evolution rate and higher species diversity than basidiomycetes[J].Scientia Sinica (Vitae),2010,40(8):731-737,765-772.(in Chinese)
[9] 韩旭峰.日龄、日粮精粗比对陕北白绒山羊瘤胃微生物区系影响的研究[D].博士学位论文.杨凌:西北农林科技大学,2015. HAN X F.Effects of age and dietary forage-to-concentrate ratios on rumen microbial flora of the Shaanbei white-cashmere goat[D].Ph.D.Thesis.Yangling:Northwest Agriculture and Forestry University,2015.(in Chinese)
[10] 戚如鑫.稻草与白菜尾菜混贮品质、微生物区系和体外发酵性能的研究[D].硕士学位论文.扬州:扬州大学,2020. QI R X.Study on the quality,microflora and in vitro fermentation performance of mixed silage of rice straw and Chinese cabbage waste[D].Master’s Thesis.Yangzhou:Yangzhou University,2020.(in Chinese)
[11] 马欣,罗珠珠,张耀全,等.黄土高原雨养区不同种植年限紫花苜蓿土壤真菌群落的分布特征[J].干旱地区农业研究,2021,39(3):162-170. MA X,LUO Z Z,ZHANG Y Q,et al.Distribution characteristics of soil fungal community in alfalfa field with different standing ages on the rain-fed Loess Plateau[J].Agricultural Research in the Arid Areas,2021,39(3):162-170.(in Chinese)
[12] ALEXANDER H M.Disease in natural plant populations,communities,and ecosystems:insights into ecological and evolutionary processes[J].Plant Disease,2010,94(5):492-503.  
[13] GALLO A,GIUBERTI G,FRISVAD J C,et al.Review on mycotoxin issues in ruminants:occurrence in forages,effects of mycotoxin ingestion on health status and animal performance and practical strategies to counteract their negative effects[J].Toxins,2015,7(8):3057-3111.  
[14] 韦玥瑞.日粮类型对内蒙古绒山羊瘤胃甲烷产生及微生物多样性影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2020. WEI Y R.Effects of diet types on rumen methane production and microbial diversity is Inner Mongolia cashmere goats[D].Master’s Thesis.Hohhot:Inner Mongolia Agricultural University,2020.(in Chinese)
[15] BAO Z H,MATSUSHITA Y,MORIMOTO S,et al.Decrease in fungal biodiversity along an available phosphorous gradient in arable Andosol soils in Japan[J].Canadian Journal of Microbiology,2013,59(6):368-373.  
[16] 戴玉成,杨祝良.中国药用真菌名录及部分名称的修订[J].菌物学报,2008,27(6):801-824. DAI Y C,YANG Z L.A revised checklist of medicinal fungi in China[J].Mycosystema,2008,27(6):801-824.(in Chinese)
[17] 戴玉成,周丽伟,杨祝良,等.中国食用菌名录[J].菌物学报,2010,29(1):1-21. DAI Y C,ZHOU L W,YANG Z L,et al.A revised checklist of edible fungi in China[J].Mycosystema,2010,29(1):1-21.(in Chinese)
[18] 左锦辉,贡晓燕,董银卯,等.蛹虫草的活性成分和药理作用及其应用研究进展[J].食品科学,2018,39(21):330-339. ZUO J H,GONG X Y,DONG Y M,et al.Research achievements in bioactive components,pharmacological effects and applications of Cordyceps militaris[J].Food Science,2018,39(21):330-339.(in Chinese)
[19] 王晟楠.鹿科动物胃内微生物菌群结构及其功能分析[D].博士学位论文.哈尔滨:东北林业大学,2020. WANG S N.Analysis on microbiome structure and function in stomach of cervidae[D].Ph.D.Thesis.Harbin:Northeast Forestry University,2020.(in Chinese)
[20] 杨顺,杨婷,林斌,等.两株溶磷真菌的筛选、鉴定及溶磷效果的评价[J].微生物学报,2018,58(2):264-273. YANG S,YANG T,LIN B,et al.Isolation and evaluation of two phosphate-dissolving fungi[J].Acta Microbiologica Sinica,2018,58(2):264-273.(in Chinese)
[21] CAO B B,JIN X,YANG H J,et al.Microbial release of ferulic and p-coumaric acids from forages and their digestibility in lactating cows fed total mixed rations with different forage combinations[J].Journal of the Science of Food and Agriculture,2016,96(2):650-655.  
[22] SIGOILLOT J C,BERRIN J G,BEY M,et al.Fungal strategies for lignin degradation[M]//JOUANIN L,LAPIERRE C.Advances in botanical research.Amsterdam:Elsevier,2012:263-308.
[23] IGIEHON N O,BABALOLA O O.Biofertilizers and sustainable agriculture:exploring arbuscular mycorrhizal fungi[J].Applied Microbiology and Biotechnology,2017,101(12):4871-4881.  
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