含尿素饲粮中添加果寡糖对幼兔氮利用、血液尿素氮向盲肠微生物氮转移的影响

晓敏; 坂口英; 弓剑

doi:10.3969/j.issn.1006-267x.2022.04.051

动物营养学报 >

2022 , Vol. 34 >Issue 4: 2575 - 2585

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.051

研究论文 RESEARCH PAPER

含尿素饲粮中添加果寡糖对幼兔氮利用、血液尿素氮向盲肠微生物氮转移的影响

晓敏 ,
坂口英 ,
弓剑

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1. 内蒙古师范大学生命科学与技术学院, 内蒙古自治区高等学校生物多样性保护与可持续利用重点实验室, 呼和浩特 010022;
2. 日本国冈山大学自然科学研究科, 冈山 7008530

晓敏(1983-),男,内蒙古音德尔人,讲师,博士,从事饲料添加剂研究。E-mail:aoxiaomin0326@163.com

收稿日期: 2021-09-29

网络出版日期: 2022-04-14

基金资助

内蒙古自然科学基金项目（2016BS0310，2020LH03011）

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Effects of Urea Containing Diet Added Fructooligosaccharides on Nitrogen Utilization and Transfer of Blood Urea Nitrogen to Cecal Microbial Nitrogen of Young Rabbits

XIAO Min ,
SAKGUCHI Ei ,
GONG Jian

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1. Key Laboratory of Biodiversity conservation and Sustainable Utilization for College and University of Inner Mongolia Autonomous Region, College of Life Sciences and Technology, Inner Mongolia Normal University, Hohhot 010022, China;
2. Graduate School of Natural Science and Technology, Okayama University, Okayama 7008530, Japan

Received date: 2021-09-29

Online published: 2022-04-14

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摘要

本试验旨在研究含尿素饲粮中添加果寡糖（FOS）对荷兰幼兔氮利用、血液尿素氮向盲肠微生物氮转移的影响。试验1:选择3月龄左右的幼兔10只，随机分为2组，每组5个重复，每个重复1只。2组分别在基础饲粮中添加10 g/kg尿素+50 g/kg葡萄糖（对照组）和10 g/kg尿素+50 g/kg FOS（试验组）。预试期3 d，正试期5 d。试验2:选择3月龄左右的幼兔12只，随机分为2组，每组6个重复，每个重复1只。2组分别饲喂与试验1相同的饲粮。试验期为9 d。屠宰前2 h试验动物分别口服2 g葡萄糖或FOS与40 mg ¹⁵N-尿素混合的3 mL水溶液。所有的试验动物于08:00-09:00麻醉，并剖腹由背大动脉抽取动脉血处死。结果显示:1）试验组的氮沉积、基于摄入量的氮沉积和基于表观消化量的氮沉积均显著高于对照组（P<0.05）。2组之间营养物质表观消化及摄入氮、粪氮和尿氮均无显著差异（P>0.05）。2）试验组的盲肠内容物重量和盲肠内容物重量/体重显著高于对照组（P<0.05），试验组的盲肠内容物丙酸浓度显著高于对照组（P<0.05），试验组的盲肠内容物有机酸总量显著高于对照组（P<0.05）。3）试验组的近端结肠内容物总氮的¹⁵N过显现量和¹⁵N添加量的百分比显著低于对照组（P<0.05）。4）试验组肝脏内容物总蛋白氮含量显著低于对照组（P<0.05），试验组盲肠内容物总蛋白氮含量显著高于对照组（P<0.05）。5）试验组近端结肠内容物总微生物氮含量显著低于对照组（P<0.05），试验组的近端结肠内容物微生物氮的¹⁵N过显现量和¹⁵N添加量的百分比均显著低于对照组（P<0.05）。6）试验组门静脉血血清尿素氮的¹⁵N过显现比例显著低于对照组（P<0.05）。综上所述，含有尿素饲粮中添加FOS可以显著提高荷兰幼兔体内氮沉积，但是饲粮蛋白质含量丰富时，不能使尿素转化为体内蛋白质。

关键词： 尿素; 果寡糖; 血液尿素氮; 盲肠微生物氮; 荷兰幼兔

本文引用格式

晓敏 , 坂口英 , 弓剑 . 含尿素饲粮中添加果寡糖对幼兔氮利用、血液尿素氮向盲肠微生物氮转移的影响[J]. 动物营养学报, 2022 , 34(4) : 2575 -2585 . DOI: 10.3969/j.issn.1006-267x.2022.04.051

Abstract

The purpose of this experiment was to determine the effects of urea containing diet added fructooligosaccharides (FOS) on nitrogen utilization and transfer of blood urea nitrogen to cecal microbial nitrogen of Dutch young rabbits. In experiment 1:selected 10 young rabbits aged about 3 months and randomly divided into 2 groups with 5 replicates per group and 1 rabbit per replicate. Rabbits in 2 group were fed basal diets supplemented with 10 g/kg urea+50 g/kg glucose (control group) and 10 g/kg urea+50 g/kg FOS (experimental group), respectively. The pre-experimental period lasted for 3 days, and the formal experimental period lasted for 5 days. In experiment 2:selected 12 young rabbits aged about 3 months and randomly divided into 2 groups with 6 replicates per group and 1 rabbit per replicate. Rabbits in 2 group rabbits were fed the same diets as experiment 1. The experimental period lasted for 9 days. At 2 h before slaughter, rabbits in 2 groups were orally given 3 mL aqueous solution of 2 g glucose or FOS mixed with 40 mg nitrogen 15 (¹⁵N)-urea, respectively. Rabbits in 2 groups were anesthetized from 08:00 to 09:00, and cut open the belly the dorsal artery extract arterial blood to be execute. The results showed as follow:1) the nitrogen deposition, nitrogen deposition based on intake and nitrogen deposition based on apparent digestion of experimental group were significantly higher than those of the control group (P<0.05). The nutrient apparent digestibility and nitrogen intake, feces nitrogen and urine nitrogen were no significant differences between 2 groups (P>0.05). 2) The cecal content weight and cecal content weight/body weight of experimental group were significantly higher than those of the control group (P<0.05), the cecal content propionic acid concentration of experimental group was significantly higher than that of the control group (P<0.05), and the cecal content organic acid total amount of experimental group was significantly higher than that of the control group (P<0.05). 3) The proportion of ¹⁵N excess and percentage of ¹⁵N addition amount of total nitrogen in proximal colon content of experimental group were significantly lower than those of the control group (P<0.05). 4) The content of total protein nitrogen in liver content of experimental group was significantly lower than that of the control group (P<0.05), and the content of total protein nitrogen in cecal content of experimental group was significantly higher than that of the control group (P<0.05). 5) The content of total microbial nitrogen in proximal colon content of experimental group was significantly lower than that of the control group (P<0.05), and the proportion of ¹⁵N excess and percentage of ¹⁵N addition amount of microbial nitrogen in proximal colon content of experimental group were significantly lower than those of the control group (P<0.05). 6) The proportion of ¹⁵N excess of urea nitrogen in portal blood serum of experimental group was significantly lower than that of the control group (P<0.05). In conclusion, the urea containing diet added FOS can significantly increase the body nitrogen deposition of Dutch young rabbits, but when the diet is rich in protein, the urea cannot be converted into body protein.

Key words： urea; fructooligosaccharides; blood urea nitrogen; cecal microbial nitrogen; Dutch young rabbits

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