研究论文 RESEARCH PAPER

苜蓿和小麦秸秆组合对断奶羔羊生长性能、肝脏糖、脂代谢相关基因表达和瘤胃黏膜形态的影响

  • 张剑霞 ,
  • 赵子鑫 ,
  • 杜瑞平 ,
  • 宋利文 ,
  • 胡红莲 ,
  • 赵濛 ,
  • 贺志雄 ,
  • 高民
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  • 1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
    2. 内蒙古自治区农牧业科学院动物营养与饲料研究所, 呼和浩特 010031;
    3. 中国科学院亚热带农业生态研究所, 中国科学院亚热带 农业生态过程重点实验室, 长沙 410125
张剑霞(1997-),女,内蒙古乌兰察布人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:1787727548@qq.com

收稿日期: 2021-09-16

  网络出版日期: 2022-04-14

基金资助

中国科学院战略性先导科技专项(XDA26050102);中国科学院STS项目“优质草原羊全产业链高效生产体系构建与示范”(KFJ-STS-ZDTP-075-2-01)

Effects of Combination of Alfalfa and Wheat Straw on Growth Performance, Expression of Genes Related to Liver Glucose and Lipid Metabolism and Rumen Mucosal Morphology of Weaned Lambs

  • ZHANG Jianxia ,
  • ZHAO Zixin ,
  • DU Ruiping ,
  • SONG Liwen ,
  • HU Honglian ,
  • ZHAO Meng ,
  • HE Zhixiong ,
  • GAO Min
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  • 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Institute of Animal Nutrition and Feed Science, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;
    3. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Chinese Academy of Sciences, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received date: 2021-09-16

  Online published: 2022-04-14

摘要

本试验旨在研究苜蓿和小麦秸秆不同组合效应对呼伦贝尔断奶羔羊生长性能、血清生化指标、肝脏糖、脂代谢相关基因表达、瘤胃黏膜形态的影响。选择体重相近、体况良好的3月龄左右断奶羔羊72只,随机分为3组,每组3个重复,每个重复8只,分别为小麦秸秆组(WS组)、50%小麦秸秆+50%苜蓿干草组(AHWS组)、苜蓿干草组(AH组),饲粮精粗比为7:3,试验期为90 d。试验结束后随机挑选7只进行屠宰并采集相关组织进行后续测定。结果显示:1)在饲粮精粗比7:3条件下,AHWS组的平均日增重高于WS组(P>0.05)和AH组(P<0.05),料重比AH组显著高于WS组和AHWS组(P<0.05),AHWS组与WS组间无显著差异(P>0.05)。2)血清中总蛋白(TP)、白蛋白(ALB)、尿素氮(UN)、葡萄糖(GLU)、总胆固醇(CHOL)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)含量及谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、淀粉酶(AMS)活性3组间均差异不显著(P>0.05),WS组血清甘油三酯(TG)含量显著高于AH组(P<0.05),与AHWS组差异不显著(P>0.05)。3)肝脏糖代谢中葡萄糖-6-磷酸酶(G6PC)、丙酮酸羧化酶(PC)、磷酸烯醇式丙酮酸羧激酶1(PCK1)基因相对表达量各组间差异不显著(P>0.05),脂肪代谢中脂肪醛脱氢酶(ALDH3A2)基因相对表达量各组间差异不显著(P>0.05),AH组脂肪酸合成酶(FASN)基因相对表达量显著高于WS组和AHWS组(P<0.05)。4)苜蓿和小麦秸秆比例为1:1组合时,可以显著提高羔羊瘤胃乳头长度(P<0.05)。综上所述,本试验条件下用50%小麦秸秆与50%苜蓿干草组合可提高呼伦贝尔断奶羔羊生长性能,促进肝脏糖、脂代谢和瘤胃乳头的发育,改善动物的生产效益。

本文引用格式

张剑霞 , 赵子鑫 , 杜瑞平 , 宋利文 , 胡红莲 , 赵濛 , 贺志雄 , 高民 . 苜蓿和小麦秸秆组合对断奶羔羊生长性能、肝脏糖、脂代谢相关基因表达和瘤胃黏膜形态的影响[J]. 动物营养学报, 2022 , 34(4) : 2613 -2625 . DOI: 10.3969/j.issn.1006-267x.2022.04.055

Abstract

This experiment was conducted to investigate the effects of different combinations of alfalfa and wheat straw on growth performance, serum biochemical indices, liver glucose and lipid metabolism related gene expression and rumen mucosal morphology of Hulunbuir weaned lambs. Seventy-two 3-month-old weaned lambs with similar body weight and good body condition were randomly divided into 3 groups with 3 replicates in each group and 8 lambs in each replicate group, which were wheat straw group (WS group), 50% wheat straw and 50% alfalfa hay group (AHWS group) and alfalfa hay group (AH group), respectively. The concentrate to forage ratio was 7:3. The experiment lasted for 90 days. After the experiment, 7 lambs were randomly selected for slaughter and related tissues were collected for subsequent determination. The results showed as follows:1) the daily gain in AHWS group was higher than that in WS group (P>0.05) and AH group (P<0.05), the ratio of feed to gain in AH group was significantly higher than that in WS and AHWS groups (P<0.05), but there was no significant difference between AHWS group and WS group (P>0.05). 2) The contents of total protein (TP), albumin (ALB), urea nitrogen (UN), glucose (GLU), total cholesterol (CHOL), low density lipoprotein (LDL), high density lipoprotein (HDL), the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and amylase (AMS) in serum were no significant difference among the three groups (P>0.05), and serum triglyceride (TG) content in WS group was significantly higher than that in AH group (P<0.05), but there was no significant difference between WS group and AHWS group (P>0.05). 3) The relative expression levels of glucose-6-phosphatase (G6PC), pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase 1 (PCK1) in liver glucose metabolism were not significantly different (P>0.05), there was no significant difference in the relative expression level of aliphatic aldehyde dehydrogenase (ALDH3A2) in lipid metabolism (P>0.05), the relative expression level of fatty acid synthetase (FASN) in AH group was significantly higher than that in WS group and AHWS group (P<0.05). 4) When the ratio of alfalfa to wheat straw was 1:1, the length of rumen papilla of lambs could be significantly increased (P<0.05). In this study, replacing wheat straw with 50% alfalfa can improve the growth performance, liver glucose and lipid metabolism, rumen papilla development, and improve the production efficiency of Hulunbuir lambs.

参考文献

[1] 高民.反刍动物饲料间的组合效应[J].内蒙古畜牧科学,1997(3):20-22. GAO M.Combination effect between feeds of ruminants[J].Animal Husbandry and Feed Science,1997(3):20-22.(in Chinese)
[2] HADJIGEORGIOU I E,GORDON I J,MILNE J A.The intake and digestion of a range of temperate forages by sheep and fibre-producing goats[J].Small Ruminant Research,2001,39(2):167-179.  
[3] OBEIDAT B S,SUBIH H S,TAYLOR J B,et al.Feeding alfalfa hay improves nursing performance of Awassi ewes and performance of growing lambs when used as a source of roughage compared with wheat straw[J].Journal of Animal Science,2018,96(Suppl-1): 32-33.
[4] KONG Y H,XIA Y,SEVIOUR R,et al.Biodiversity and composition of methanogenic populations in the rumen of cows fed alfalfa hay or triticale straw[J].FEMS Microbiology Ecology,2013,84(2):302-315.  
[5] HADDAD S G.Associative effects of supplementing barley straw diets with alfalfa hay on rumen environment and nutrient intake and digestibility for ewes[J].Animal Feed Science and Technology,2000,87(3/4): 163-171.
[6] 吕明,张甜甜,王平,等.早期补饲苜蓿对哺乳奶山羊羔羊生长发育及血液生化指标的影响[C]//2018年全国养羊生产与学术研讨会论文集.蚌埠:[s.n.],2018:218. LYU M,ZHANG T T,WANG P,et al.Effects of early supplementation of alfalfa on growth and blood biochemical indices of lactating goat lambs[C]//Proceedings of the 2018 National Sheep Production and Academic Symposium.Bengbu:[s.n.],2010:218.
[7] 谢小来,高会江,包军,等.不同全混合日粮羔羊育肥试验[J].饲料博览,200410):38-40. XIE X L,GAO H J,BAO J,et al.Study on lamb feeding with different total mixed rations[J].Feed Review,2004(10):38-40.(in Chinese)
[8] BEN-GHEDALIA D,MIRON J.Digestion by sheep of monosaccharide constituents of direct cut alfalfa silage made with SO2-treated wheat straw[J].Animal Feed Science and Technology,2001,92(3/4):175-183.
[9] 中华人民共和国农业部.肉羊饲养标准:NY/T 816—2004[S].北京:中国农业出版社,2004. Ministry of Agriculture of the People’s Republic of China.Feeding standard of meat-producing sheep and goats:NY/T 816—2004[S].Beijing:China Agricultural Press,2004. (in Chinese)
[10] 熊本海,罗清尧,郑姗姗,等.中国饲料成分及营养价值表(2020年第31版)制订说明[J].中国饲料,2020(21):87-97. XIONG B H,LUO Q Y,ZHENG S S,et al.Introduction of tables of feed composition and nutritive values in China (2020 thirty-first edition)[J].China Feed,2020(21):87-97.(in Chinese)
[11] 祁敏丽.日粮能量和蛋白质水平对羔羊生长性能和胃肠道发育的影响[D].硕士学位论文.北京:中国农业科学院,2016. QI M L.Effects of dietary energy and protein levels on growth performance and gastrointestinal development of lambs[D].Master’s Thesis.Beijing:Chinese Academy of Agricultural Sciences,2016.(in Chinese)
[12] 吕小康.不同饲粮对20-60日龄山羊羔羊瘤胃发育的影响[D].硕士学位论文.北京:中国农业科学院,2019. LV X K.Effects of different diets on rumen development of 20-60 day-old goat kids[D].Master’s Thesis.Beijing:Chinese Academy of Agricultural Sciences,2019.(in Chinese)
[13] 王富伟,曾浩,苏华维,等.优质苜蓿替代部分精料对泌乳奶牛生产性能、消化代谢和血液生化指标的影响[J].中国畜牧杂志,2011,47(11):41-44. WANG F W,ZENG H,SU H W,et al.Effects of quality alfalfa replacing part of concentrate on performance, digestion and metabolism and blood biochemical indices of lactating dairy cows[J].Chinese Journal of Animal Science,2011,47(11):41-44.(in Chinese)
[14] 樊文娜,韩康康,李栋栋,等.苜蓿草粉对波尔山羊生长性能的影响及经济效益分析[J].动物营养学报,2017,29(9):3301-3307. FAN W N,HAN K K,LI D D,et al.Effects of alfalfa meal on growth performance of Boer goats and economic benefits analysis[J].Chinese Journal of Animal Nutrition,2017,29(9):3301-3307.(in Chinese)
[15] 姜碧薇,王甜,周玉香,等.酶菌混合处理荞麦秸秆和苜蓿干草对滩羊生长性能、屠宰性能、瘤胃细菌多样性和碳水化合物活性酶类的影响[J].动物营养学报,2021,33(4):2335-2346. JIANG B W,WANG T,ZHOU Y X,et al.Effects of buckwheat straw and alfalfa hay treated by enzyme and bacteria on growth performance,slaughter performance,rumen bacterial diversity and carbohydrate-active enzymes of Tan sheep[J].Chinese Journal of Animal Nutrition,2021,33(4):2335-2346.(in Chinese)
[16] 姜碧薇,周玉香,王甜,等.酶菌混合处理稻草和苜蓿干草对滩羊生长性能、血清生化指标、瘤胃细菌多样性及KEGG通路的影响[J].动物营养学报,2021,33(3):1482-1492. JIANG B W,ZHOU Y X,WANG T,et al.Effects of straw and alfalfa hay treated by enzyme and bacteria on growth performance,serum biochemical indexes,rumen bacterial diversity and KEGG pathway of Tan sheep[J].Chinese Journal of Animal Nutrition,2021,33(3):1482-1492.(in Chinese)
[17] 庄涛,顾洪如,赵国琦,等.不同粗饲料组合对波尔×徐淮山羊生长和屠宰性能的影响[J].中国畜牧杂志,2012,48(15):41-43. ZHUANG T,YI H R,ZHAO G Q,et al.Effects of different roughage combinations on growth and slaughter performance of Boer×Xuhuai goats[J].Chinese Journal of Animal Science,2012,48(15):41-43.(in Chinese)
[18] 靳光,薛艳蓉,张元庆,等.不同粗饲料与玉米青贮组合对晋南牛生长性能、屠宰性能、产肉性能及肉品质的影响[J].动物营养学报,2021,33(10):5653-5663. JIN G,XUE Y R,ZHANG Y Q,et al.Effects of different combinations of roughage and corn silage on growth performance,slaughter performance,meat performance and meat quality of Jinnan cattle[J].Chinese Journal of Animal Nutrition,2021,33(10):5653-5663.(in Chinese)
[19] 张丹丹,张元庆,程景,等.不同粗饲料组合对晋南牛瘤胃体外发酵特性的研究[J].草业学报,2021,30(7):93-100. ZHANG D D,ZHANG Y Q,CHENG J,et al.Effects of different roughage combinations on in vitro rumen fermentation characteristics of Jinnan cattle[J].Acta Prataculturae Sinica,2021,30(7):93-100.(in Chinese)
[20] THEURER C B,SWINGLE R S,WANDERLEY R C,et al.Sorghum grain flake density and source of roughage in feedlot cattle diets[J].Journal of Animal Science,1999,77(5):1066-1073.  
[21] 刘丽英,王志军,尹强,等.3种饲草不同配比的体外消化特性及组合效应分析[J].畜牧兽医学报,2017,48(6):1066-1075. LIU L Y,WANG Z J,YIN Q,et al.Research on the in vitro digestibility and associative effects of three forage with different proportion[J].Acta Veterinaria et Zootechnica Sinica,2017,48(6):1066-1075.(in Chinese)
[22] OBITSU T,GOTO M,SUGINO T,et al.The effect of dietary ratios of corn silage and alfalfa hay on carbohydrate digestion and retention time of feed particles in the gastrointestinal tract of steers[J].Animal Science Journal,2009,80(5):546-555.  
[23] 韩肖敏,李建国,李秋凤,等.体外法评价玉米秸秆、小麦秸秆与玉米秸秆青贮的组合效应[J].家畜生态学报,2017,38(7):44-49. HAN X M,LI J G,LI Q F,et al.Associative effects of cornstalk,wheat straw and corn stalk silage by gas production technique in vitro[J].Acta Ecologae Animalis Domastici,2017,38(7):44-49.(in Chinese)
[24] 金磊,王立志.肠道微生物与宿主免疫关系研究进展[J].现代畜牧兽医,2018(9):52-58. JIN L,WANG Z L.Research progress in the relationship between intestinal microbes and host immunity[J].Modern Animal Husbandry and Veterinary Medicine,2018(9):52-58.(in Chinese)
[25] 姜婉茹,纪晓霞,高铭谣,等.2种饲草对湖羊增重、采食量及血液生化指标的影响[J].中国兽医学报,2020,40(6):1209-1215. JIANG W R,JI X X,GAO M Y,et al.Effects of two forages on weight gain,feed intake and blood biochemical indexes of Hu sheep[J].Chinese Journal of Veterinary Science,2020,40(6):1209-1215.(in Chinese)
[26] 刘旭蕾,康颖,徐磊,等.不同紫花苜蓿添加比例对断奶绒山羊公羔生长性能的影响[J].家畜生态学报,2019,40(7):50-54. LIU X L,KANG Y,XU L,et al.Effects of different alfalfa addition ratios on growth performance of weaned male cashmere goats[J].Acta Ecologae Animalis Domastici,2019,40(7):50-54.(in Chinese)
[27] 王灿,苗志敏,李长贵,等.人体血尿酸水平对血清谷丙转氨酶和谷草转氨酶水平的影响[J].山东医药,2010,50(29):1-3. WANG C,MIAO Z M,LI C G,et al.Impact of uric acid on alanine amiotransferase and aspartate aminotransferase[J].Shandong Medical Journal,2010,50(29):1-3.(in Chinese)
[28] 陈希,姜婉茹,高铭谣,等.不同粗饲料苜蓿干草和稻秸对湖羊饲喂效果的比较研究[J].动物营养学报,2020,32(11):5285-5292. CHEN X,JIANG W R,GAO M Y,et al.Comparative study on feeding effect of different roughages alfalfa hay and rice straw on Hu sheep[J].Chinese Journal of Animal Nutrition,2020,32(11):5285-5292.(in Chinese)
[29] MINUTI A,ZHOU Z,GRAUGNARD D E,et al.Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows[J].Physiological Reports,2015,3(4):e12388.
[30] STANLEY C C,WILLIAMS C C,JENNY B F,et al.Effects of feeding milk replacer once versus twice daily on glucose metabolism in Holstein and Jersey calves[J].Journal of Dairy Science,2002,85(9):2335-2343.  
[31] 薛丰,杜晋平,解祥学,等.玉米和玉米青贮日粮添加赖氨酸对肉牛生长性能及血液生化指标的影向[J].中国畜牧杂志,2010,46(19):38-41. XUE F,DU J P,XIE X X,et al.Effect of supplementation of rumen-protected lysine on growing performance and blood biochemical indices of beef cattle fed cornstalk silage/corn grain-based diet[J].Chinese Journal of Animal Science,2010,46(19):38-41.(in Chinese)
[32] 张颖,毛华明.不同粗饲料日粮对云岭黑山羊蛋白质代谢相关血液指标的影响[J].饲料研究,2015(3):44-47. ZHANG Y,MAO H M.Effects of different roughages on blood indices related to protein metabolism of Yunling black goats[J].Feed Research,2015(3):44-47.(in Chinese)
[33] 杨芳.苜蓿皂甙对小尾寒羊生产性能、血清生化指标及免疫功能的影响[D].硕士学位论文.洛阳:河南科技大学,2015. YANG F.Effects of alfalfa saponin on the production performance,serum biochemical indexes,and immune function in small tailed Han sheep[D].Master’s Thesis.Luoyang:Henan University of Science and Technology,2015.(in Chinese)
[34] SEMAKOVA J,HYROŠŠOVÁ P,MÉNDEZ-LUCAS A,et al.PEPCK-C reexpression in the liver counters neonatal hypoglycemia in Pck1del/del mice,unmasking role in non-gluconeogenic tissues[J].Journal of Physiology and Biochemistry,2017,73(1):89-98.  
[35] 高燕,杨改青,王林枫,等.杜仲叶对绵羊肝脏糖代谢及其相关基因表达的影响[J].动物营养学报,2019,31(6):2854-2864. GAO Y,YANG G Q,WANG L F,et al.Effects of Eucommia ulmoides leaves on glucose metabolism and related gene expression in sheep liver[J].Chinese Journal of Animal Nutrition,2019,31(6):2854-2864.(in Chinese)
[36] WANG B,ZHAO F Q,ZHANG B X,et al.An insufficient glucose supply causes reduced lactose synthesis in lactating dairy cows fed rice straw instead of alfalfa hay[J].Journal of Animal Science,2016,94(11):4771-4780.  
[37] 张吉鹍.反刍动物秸秆基础日粮补饲苜蓿的组合效应[J].中国奶牛,2007(5):16-19. ZHANG J K.Associative effects of supplementing ruminant straw diets with alfalfa hay[J].China Dairy Cattle,2007(5):16-19.(in Chinese)
[38] 腾桥,唐金金,夏丽洁.FASN基因的原核表达及生物信息学分析[J].基因组学与应用生物学,2020,39(3):995-1002. TENG Q,TANG J J,XIA L J.Prokaryotic expression and bioinformatics analysis of FASN gene[J].Genomics and Applied Biology,2020,39(3):995-1002.(in Chinese)
[39] GARCÍA-FERNÁNDEZ M,GUTIÉRREZ-GIL B,GARCÍA-GÁMEZ E,et al.The identification of QTL that affect the fatty acid composition of milk on sheep chromosome 11[J].Animal Genetics,2010,41(3):324-328.  
[40] BUCKLEY D,DUKE G,HEUER T S,et al.Fatty acid synthase-modern tumor cell biology insights into a classical oncology target[J].Pharmacology & Therapeutics,2017,177:23-31.
[41] SMITH S,WITKOWSKI A,JOSHI A K.Structural and functional organization of the animal fatty acid synthase[J].Progress in Lipid Research,2003,42(4):289-317.  
[42] HANSMANNEL F,MORDIER S,IYNEDJIAN P B.Insulin induction of glucokinase and fatty acid synthase in hepatocytes:analysis of the roles of sterol-regulatory-element-binding protein-1c and liver X receptor[J].Biochemical Journal,2006,399(2):275-283.  
[43] PECKETT A J,WRIGHT D C,RIDDELL M C.The effects of glucocorticoids on adipose tissue lipid metabolism[J].Metabolism,2011,60(11):1500-1510.  
[44] MILDNER A M,CLARKE S D.Porcine fatty acid synthase:cloning of a complementary DNA,tissue distribution of its mRNA and suppression of expression by somatotropin and dietary protein[J].The Journal of Nutrition,1991,121(6):900-907.  
[45] 田卫华,杨丽玉,李红,等.蛋鸡脂肪代谢相关基因ACACAFASN表达及调控特性[J].农业生物技术学报,2019,27(2):280-288. TIAN W H,YANG L Y,LI H,et al.Expression and regulation characteristics of lipid metabolism-related ACACA and FASN genes in laying hens (gallus gallus)[J].Journal of Agricultural Biotechnology,2019,27(2):280-288.(in Chinese)
[46] BERTRAM J H,MULLINER K M,SHI K,et al.Five fatty aldehyde dehydrogenase enzymes from Marinobacter and Acinetobacter spp. and structural insights into the aldehyde binding pocket[J].Applied and Environmental Microbiology,2017,83(12):e00018-17.
[47] 张雄,史开志,张勇,等.猪FASN基因表达与胴体及肉质性状相关性研究[J].黑龙江畜牧兽医,2021(9):64-67. ZHANG X,SHI K Z,ZHANG Y,et al.Study on the correlation between FASN gene expression and carcass and meat quality traits in pigs[J].Heilongjiang Animal Science and Veterinary Medicine,2021(9):64-67.(in Chinese)
[48] BEHARKA A A,NAGARAJA T G,MORRILL J L,et al.Effects of form of the diet on anatomical,microbial,and fermentative development of the rumen of neonatal calves[J].Journal of Dairy Science,1998,81(7):1946-1955.  
[49] LAVKER R M,MATOLTSY A G.Formation of horny cells:the fate of cell organelles and differentiation products in ruminal epithelium[J].Journal of Cell Biology,1970,44(3):501-512.  
[50] SUÁREZ B J,VAN REENEN C G,STOCKHOFE N,et al.Effect of roughage source and roughage to concentrate ratio on animal performance and rumen development in veal calves[J].Journal of Dairy Science,2007,90(5):2390-2403.  
[51] 孙若琳,梁栋,成艳芬,等.饲喂麦秸对湖羊消化道上皮形态的影响[J].畜牧与兽医,2019,51(8):26-29. SUN R L,LIANG D,CHENG Y F,et al.Effects of wheat straw on the intestinal morphology of Hu sheep[J].Animal Husbandry & Veterinary Medicine,2019,51(8):26-29.(in Chinese)
[52] HADJIGEORGIOU I E,GORDON I J,MILNE J A.Intake,digestion and selection of roughage with different staple lengths by sheep and goats[J].Small Ruminant Research,2003,47(2):117-132.  
[53] KRAUSE K M,COMBS D K.Effects of forage particle size,forage source,and grain fermentability on performance and ruminal pH in midlactation cows[J].Journal of Dairy Science,2003,86(4):1382-1397.  
[54] 王娇,杨宝钰,阿依古丽艾买尔,等.日粮添加甜高粱与苜蓿混贮对卡拉库尔羊消化道酶活性的影响[J].中国畜牧杂志,2021,57(1):138-144. WANG J,YANG B Y,AygulAmar,et al.Effect of dietary supplementation of sweet sorghum and alfalfa silage mixtures on enzyme activity of digestive tract of Karakul sheep[J].Chinese Journal of Animal Science,2021,57(1):138-144.(in Chinese)
[55] 李俊,吴建国,郭慧慧.不同水平苜蓿添加量对断奶前羔羊生长性能和瘤胃发育的影响[J].中国饲料,2018(10):72-76. LI J,WU J G,GUO H H. Effects of different levels of alfalfa hay on growth performance and rumen development of pre-weanling lambs[J].China Feed,2018(10):72-76(in Chinese).
[56] 刘洪亮,娄玉杰.羊草和苜蓿草产品营养物质瘤胃降解特性的研究[J].中国草地学报,2006,28(6):47-51. LIU H L,LOU Y J.Study on degradation character of leymus chinensis and alfalfa products in the cow rumen[J].Chinese Journal of Grassland,2006,28(6):47-51.(in Chinese)
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