研究论文 RESEARCH PAPER

不同提取方法的桑叶提取物对小鼠生长性能、抗氧化和免疫功能的作用研究

  • 黄慧 ,
  • 夏鑫 ,
  • 周应军 ,
  • 刘莹莹 ,
  • 王贤泽 ,
  • 王霞 ,
  • 肖定福
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  • 1. 湖南农业大学动物科学技术学院, 长沙 410128;
    2. 湖南今汉药业有限公司, 长沙 410014;
    3. 湖南省畜牧兽医研究所, 长沙 410131
黄慧(1997-),女,福建三明人,硕士研究生,从事动物营养生理方面的研究。E-mail:15280597809@163.com

收稿日期: 2021-09-18

  网络出版日期: 2022-04-14

基金资助

湖南省自然科学基金(2020JJ4364);中国博士后科学基金面上项目(2019M652772);湖南省科技特派员创新创业项目(2020NK4247);湖南农业大学与湖南今汉药业有限公司合作项目(2019xny-js065);湖南农业大学与湖南双峰县农业农村局合作项目(0221019)

Effects of Mulberry Leaf Extracts with Different Extraction Methods on Growth Performance, Antioxidant and Immune Function in Mice

  • HUANG Hui ,
  • XIA Xin ,
  • ZHOU Yingjun ,
  • LIU Yingying ,
  • WANG Xianze ,
  • WANG Xia ,
  • XIAO Dingfu
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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Geneham Pharmaceutical Co., Ltd., Changsha 410014, China;
    3. Hunan Institute of Animal and Veterinary Science, Changsha 410131, China

Received date: 2021-09-18

  Online published: 2022-04-14

摘要

本试验旨在通过探究不同桑叶提取段及桑叶粗提取物对小鼠生长性能、抗氧化功能、血清生化指标和炎症细胞因子的影响,筛选出一种较有效的桑叶提取方法。试验使用正己烷、乙酸乙酯、乙醇(85%)和水(60~70℃)制得桑叶提取物,另外使用水煎煮的方法制得桑叶粗提物,分别记为A、B、C、D和E。选取108只5周龄无特定病原体(SPF)级ICR雄性小鼠,随机分为6组,对照组饲喂基础饲粮,试验组在基础饲粮中分别添加桑叶提取物A、B、C、D和E(1 200 mg/kg),每组6个重复,每个重复3只小鼠,预试期3 d,正试期28 d。结果显示:与对照组相比,1)E组小鼠的平均日采食量(ADFI)极显著提高(P<0.01);2)C、D和E组小鼠血清谷胱甘肽过氧化物酶(GSH-Px)活性显著升高(P<0.05),B、D和E组血清总超氧化物歧化酶(T-SOD)活性极显著升高(P<0.01);血清高密度脂蛋白胆固醇(HDL-C)含量显著降低(P<0.05),B和D组血清天门冬氨酸氨基转移酶(AST)活性显著升高(P<0.05),B和E组在血清甘油三酯(TG)含量显著降低(P<0.05);A、C、D、E组血清白蛋白(ALB)和总蛋白(TP)含量均显著升高(P<0.05),C组血清肌酐(CREA)含量显著降低(P<0.05);3)A、C、D、E组小鼠肝脏中白细胞介素(IL)-17含量极显著升高(P<0.01),肝脏IL-2含量极显著下降(P<0.01),A、C、D组肝脏IL-1β和干扰素-γ(IFN-γ)含量极显著升高(P<0.01),A组肝脏肿瘤坏死因子-α(TNF-α)含量显著升高(P<0.05)。综上所述,饲粮中添加1 200 mg/kg的桑叶粗提物能提高小鼠平均日采食量,增强小鼠机体抗氧化能力,有益调节血清生化指标,改善肝脏功能,激活免疫反应,初步筛选出桑叶粗提物作用效果最好。

本文引用格式

黄慧 , 夏鑫 , 周应军 , 刘莹莹 , 王贤泽 , 王霞 , 肖定福 . 不同提取方法的桑叶提取物对小鼠生长性能、抗氧化和免疫功能的作用研究[J]. 动物营养学报, 2022 , 34(4) : 2702 -2711 . DOI: 10.3969/j.issn.1006-267x.2022.04.063

Abstract

The aim of this experiment was to screen out a more effective mulberry leaf extraction method by exploring the effects of different mulberry leaf extracts and mulberry leaf crude extracts on growth performance, antioxidant function, serum biochemical indexes and inflammatory cytokines in mice. In this experiment, the mulberry leaves were percolated extracted with n-hexane, ethyl acetate, ethanol (85%) and water (60 to 70℃), and then the mulberry leaves crude extracts were extracted with water decocting method, which were extracts A, B, C,D and E. A total of 108 five-week-old specific pathogen-free (SPF) grade ICR male mice were randomly divided into 6 groups with 6 repeats and 3 mice for each repeat. The control group was fed a basal diet, and the test groups were respectively fed the basal diet supplemented with mulberry leaf extracts A, B, C, D and E (1 200 mg/kg). The pre-feeding period was 3 days, and the official trial period was 28 days. The results are as follows:compared with the control group, 1)the average daily feed intake (ADFI) of mice in group E was significantly increased (P<0.01); 2) the activity of glutathione peroxidase (GSH-Px) in serum of mice was significantly increased in groups C, D and E (P<0.05). Total superoxide dismutase (T-SOD) activity in serum was significantly increased in groups B, D and E (P<0.01), and serum high-density lipoprotein cholesterol (HDL-C) content was significantly reduced (P<0.05). Aspartate aminotransferase (AST) activity was significantly increased in mice in groups B and D (P<0.05), and serum triglycerides (TG) content in groups B and E was significantly decreased (P<0.05). Albumin (ALB) and total protein (TP) contents in serum were significantly increased in groups A, C D and E (P<0.05). Creatinine (CREA) content in serum was significantly reduced in group C (P<0.05); 3) the content of interleukin (IL)-17 in the liver of mice in groups A, C, D and E were extremely significantly increased (P<0.01), and the content of IL-2 was significantly decreased (P<0.01). The contents of IL-1β and interferon-γ (IFN-γ) in liver in groups A, C, and D were extremely significantly increased (P<0.01), and tumor necrosis factor-α (TNF-α) content in liver in group A was significantly increased (P<0.05). The results show that adding 1 200 mg/kg of mulberry leaf crude extracts to the diet can increase the average daily feed intake of mice, enhance the antioxidant capacity of mice, beneficially regulate serum biochemical indexes, improve liver function, and activate immune response. The crude mulberry leaf extract is screened out to have the best effect.

参考文献

[1] 杜周和,左艳春,严旭,等.饲料桑生理活性物质及其饲用价值[J].草业学报,2017,26(10):227-236. DU Z H,ZUO Y C,YAN X,et al.Physiological activation and feed value of mulberry for livestock and poultry[J].Acta Prataculturae Sinica,2017,26(10):227-236.(in Chinese)
[2] XV Z C,HE G L,WANG X L,et al.Mulberry leaf powder ameliorate high starch-induced hepatic oxidative stress and inflammation in fish model[J].Animal Feed Science and Technology,2021,278:115012.
[3] LIU Z M,WANG H Y,ZHANG S,et al.Analysis of active components of hexane extractives of Morus alba leaves[J].Agricultural Science&Technology,2012,12(1):23-25.
[4] 刘琳.高速逆流色谱分离桑叶乙酸乙酯部位化学成分的研究[D].硕士学位论文.北京:北京中医药大学,2014. LIU L.Study on separation of partial chemical components of ethyl acetate from mulberry leaves by high speed countercurrent chromatography[D].Master’s Thesis.Beijing:Beijing University of Chinese Medicine,2014.(in Chinese)
[5] 高欣妍,王海英,刘志明.桑叶乙醇提取物的体外抗氧化与抑菌活性[J].生物质化学工程,2019,53(2):35-40. GAO X Y,WANG H Y,LIU Z M,et al.In vitro antioxidant and antibacterial activity of ethanol extract from mulberry leaves[J].Biomass Chemical Engineering,2019,53(2):35-40.(in Chinese)
[6] 唐忠海.桑叶功能成分的分离及其水提物对Ⅰ-糖尿病小鼠治疗作用的研究[D].博士学位论文.长沙:中南大学,2011. TANG Z H.Study on the technology of extraction and the hypoglycemic effect of the functional components from Morus alba L. in Ⅰ-diabetic mice[D].Ph.D.Thesis.Changsha:Central South University,2011.(in Chinese)
[7] 曲培滨.桑叶黄酮和热带假丝酵母对犊牛生长性能、屠宰性能、肉品质及血清指标的影响[D].硕士学位论文.邯郸:河北工程大学,2015. QU P B.Effects of mulberry leaf flavonoids and candida tropical on growth performance,slaughter performance,meat quality and blood biochemical index in calves[D].Master’s Thesis.Handan:Hebei University of Engineering,2015.(in Chinese)
[8] 陈城城.桑叶化学成分研究[D].硕士学位论文.齐齐哈尔:齐齐哈尔大学,2012. CHEN C C.Study on chemical constituents of the leaves of Morus alba Linn.[D].Master’s Thesis.Qiqihar:Qiqihar University,2012.(in Chinese)
[9] HUANG C B,ALIMOVA Y,MYERS T M,et al.Short- and medium-chain fatty acids exhibit antimicrobial activity for oral microorganisms[J].Archives of Oral Biology,2011,56(7):650-654.  
[10] 闻爱友,胡洪,吴学壮,等.复合植物精油对肉鸡生产性能、消化酶活性及糖脂代谢的影响[J].安徽科技学院学报,2019,33(2):13-19. WEN A Y,HU H,WU X Z,et al.Effects of plant essential oil compound on performance,digestive enzyme activity and glucose and lipid metabolism of broilers[J].Journal of Anhui Science and Technology University,2019,33(2):13-19.(in Chinese)
[11] 陈冰,杨继华,曹俊明,等.桑叶黄酮对吉富罗非鱼肌肉抗氧化指标及营养组成的影响[J].淡水渔业,2018,48(3):90-95. CHEN B,YANG J H,CAO J M,et al.Effects of dietary mulberry leaf flavonoids on muscle antioxidant indices and nutritional compositions of GIFT,Oreochromis niloticus[J].Freshwater Fisheries,2018,48(3):90-95.(in Chinese)
[12] 杨继华.桑叶黄酮对吉富罗非鱼生长性能、抗氧化功能和肌肉品质的影响[D].硕士学位论文.广州:华南农业大学,2017. YANG J H.Effects of dietary mulberry leaf flavonoids on growth performance,antioxidant ability and muscle quality of GIFT Oreochromis niloticus[D].Master’s Thesis.Guangzhou:South China Agricultural University,2017.(in Chinese)
[13] KHAN M A,RAHMAN A A,ISLAM S,et al.A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae)[J].BMC Research Notes,2013,6:24.
[14] CHEN A Y,CHEN Y C.A review of the dietary flavonoid,kaempferol on human health and cancer chemoprevention[J].Food Chemistry,2013,138(4):2099-2107.  
[15] PATIL S L,MALLAIAH S H,PATIL R K.Antioxidative and radioprotective potential of rutin and quercetin in Swiss albino mice exposed to gamma radiation[J].Journal of Medical Physics,2013,38(2):87-92.  
[16] 李冠楠,夏雪娟,赵欢欢,等.桑叶提取物泡腾片制备工艺及其抗氧化活性研究[J].西南大学学报(自然科学版),2015,37(4):132-137. LI G N,XIA X J,ZHAO H H,et al.On preparation of original mulberry essence effervescent tablets[J].Journal of Southwest University(Natural Science),2015,37(4):132-137.(in Chinese)
[17] ANDALLU B,SHANKARAN M,ULLAGADDI R,et al.In vitro free radical scavenging and in vivo antioxidant potential of mulberry (Morus indica L.) leaves[J].Journal of Herbal Medicine,2014,4(1):10-17.  
[18] 王丽娟.桑叶提取物抑菌活性及抗氧化活性的研究[D].硕士学位论文.杭州:浙江工业大学,2012. WANG L J.Study on the antibacterial activity and antioxidant activity of mulberry leaf extract[D].Master’s Thesis.Hangzhou:Zhejiang University of Technology,2012.(in Chinese)
[19] 张贵会,王贺,杨玲.新疆药桑叶中黄酮类化合物的分离及其抗氧化活性评价[J].中国酿造,2016,35(2):101-105. ZHANG G H,WANG H,YANG L.Isolation of flavonoids compounds in Morus nigra leaves from Xinjiang and their antioxidant activity[J].China Brewing,2016,35(2):101-105.(in Chinese)
[20] KIM G N,JANG H D.Flavonol content in the water extract of the mulberry (Morus alba L.) leaf and their antioxidant capacities[J].Journal of Food Science,2011,76(6):C869-C873.
[21] 夏伯候,周亚敏,皮胜玲,等.UPLC测定桑叶中抗氧化活性成分异槲皮苷、芦丁和紫云英苷的含量[J].中药材,2016,39(3):586-589. XIA B H,ZHOU Y M,PI S L,et al.UPLC determination of the contents of antioxidant active components isoquercitrin,rutin and astragalin in mulberry leaves[J].Journal of Chinese Medicinal Materials,2016,39(3):586-589.(in Chinese)
[22] 何新苗,孟磊,李欣,等.药桑叶多糖的体外抗氧化活性研究[J].新疆医科大学学报,2018,41(6):747-750,755. HE X M,MENG L,LI X,et al.In vitro antioxidant activity of polysaccharides from Morus alba L.[J].Journal of Xinjiang Medical University,2018,41(6):747-750,755.(in Chinese)
[23] ZHANG D Y,WAN Y,XU J Y,et al.Ultrasound extraction of polysaccharides from mulberry leaves and their effect on enhancing antioxidant activity[J].Carbohydrate Polymers,2016,137:473-479.
[24] 王芳,李奕霖,莫政松,等.湘西土家药黄桑叶多糖成分体外抗氧化研究[J].广州化工,2018,46(20):61-63. WANG F,LI Y L,MO Z S,et al.Study on antioxidant activity of polysaccharides from yellow mulberry leaves of Tujia medicine in Xiangxi[J].Guangzhou Chemical Industry,2018,46(20):61-63.(in Chinese)
[25] YUAN Q X,XIE Y F,WANG W,et al.Extraction optimization,characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves[J].Carbohydrate Polymers,2015,128:52-62.
[26] 李层层,王德萍,江岩.新疆桑叶营养成分分析及体外抗氧化作用研究[J].食品工业,2015,36(5):78-82. LI C C,WANG D P,JIANG Y,et al.Nutrient analysis and in vitro anti-oxidative effects of mulberry leaves from Xinjiang Province[J].The Food Industry,2015,36(5):78-82.(in Chinese)
[27] 张入飞,蔡为荣,谢亮亮,等.桑叶多糖的分离纯化及其抗凝血活性的研究[J].安徽工程大学学报,2015,30(2):29-33. ZHANG R F,CAI W R,XIE L L,et al.Isolation,purification and anticoagulant activity of the polysaccharide from the leaves of mulberry[J].Journal of Anhui Polytechnic University,2015,30(2):29-33.(in Chinese)
[28] LI Y G,JI D F,ZHONG S,et al.Hybrid of 1-deoxynojirimycin and polysaccharide from mulberry leaves treat diabetes mellitus by activating PDX-1/insulin-1 signaling pathway and regulating the expression of glucokinase,phosphoenolpyruvate carboxykinase and glucose-6-phosphatase in alloxan-induced diabetic mice[J].Journal of Ethnopharmacology,2011,134(3):961-970.  
[29] 王玲,邹莉芳,黄先智,等.桑叶1-脱氧野尻霉素水提液对高脂饮食小鼠脂肪分解代谢的影响[J].营养学报,2018,40(4):376-380. WANG L,ZOU L F,HUANG X Z,et al.Effect of 1-deoxynojirimycin water extract from mulberry leaves on lipid catabolism in high-fat fed mice[J].Acta Nutrimenta Sinica,2018,40(4):376-380.(in Chinese)
[30] SUN X F,YAMASAKI M,KATSUBE T,et al.Effects of quercetin derivatives from mulberry leaves:improved gene expression related hepatic lipid and glucose metabolism in short-term high-fat fed mice[J].Nutrition Research and Practice,2015,9(2):137-143.  
[31] 王玲,邹莉芳,黄先智,等.桑叶水提物对高脂饮食小鼠脂代谢的调控机制初探[J].中国食品学报,2019,19(10):23-33. WANG L,ZOU L F,HUANG X Z,et al.Regulation mechanism of water extract of mulberry leaf on lipid metabolism in high-fat fed mice[J].Journal of Chinese Institute of Food Science and Technology,2019,19(10):23-33.(in Chinese)
[32] ZENI A L B,MOREIRA T D,DALMAGRO A P,et al.Evaluation of phenolic compounds and lipid-lowering effect of Morus nigra leaves extract[J].Anais da Academia Brasileira de Ciencias,2017,89(4):2805-2815.  
[33] AUJLA S J,DUBIN P J,KOLLS J K.Interleukin-17 in pulmonary host defense[J].Experimental Lung Research,2007,33(10):507-518.  
[34] SZALECKI M,MALINOWSKA A,PROKOP-PIOTRKOWSKA M,et al.Interactions between the growth hormone and cytokines—a review[J].Advances in Medical Sciences,2018,63(2):285-289.  
[35] KOMIYAMA Y,NAKAE S,MATSUKI T,et al.IL-17 plays an important role in the development of experimental autoimmune encephalomyelitis[J].Journal of Immunology,2006,177(1):566-573.  
[36] MUHAMMAD YUSOFF F,WONG K K,MOHD REDZWAN N.Th1,Th2,and Th17 cytokines in systemic lupus erythematosus[J].Autoimmunity,2020,53(1):8-20.  
[37] 陈晓兰,杨海峰,瞿静雯,等.不同桑叶提取物对小鼠脾脏淋巴细胞增殖能力的影响[J].中国畜牧兽医,2017,44(12):3598-3604. CHEN X L,YANG H F,QU J W,et al.Effect of different mulberry leaf extracts on spleen lymphocyte proliferation in mice[J].China Animal Husbandry & Veterinary Medicine,2017,44(12):3598-3604.(in Chinese)
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