动物营养学报 >

2022 , Vol. 34 >Issue 4: 2712 - 2720

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.064

研究论文 RESEARCH PAPER

芒柄花素对免疫抑制小鼠小肠黏膜免疫功能的影响

  • 毛甜甜 ,
  • 白衡 ,
  • 贾宁
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  • 甘肃农业大学动物医学院, 兰州 730070
毛甜甜(1998-),女,甘肃庆阳人,硕士研究生,从事动物疾病病理研究。E-mail:1179562509@qq.com

收稿日期: 2021-08-31

  网络出版日期: 2022-04-14

基金资助

国家自然科学基金项目（31560686）

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Effects of Formonetin on Mucosa Immunity Function in Small Intestinal of Immunosuppressive Mice

  • MAO Tiantian ,
  • BAI Heng ,
  • JIA Ning
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  • College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2021-08-31

  Online published: 2022-04-14

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摘要

本研究旨在探讨芒柄花素（FMN）对免疫抑制小鼠小肠黏膜免疫功能的影响。将50只昆明种小鼠随机分为5组，分别为空白对照组、免疫抑制模型组以及FMN低、中、高剂量组，每组10只。试验共28 d，前7 d，空白对照组小鼠每天灌胃0.6 mL生理盐水，其他组小鼠灌胃40 mg/（kg·d）环磷酰胺（CTX）0.6 mL；后21 d，空白对照组与免疫抑制模型组小鼠每天灌胃0.6 mL生理盐水，FMN低、中、高剂量组小鼠分别灌胃50、150、250 mg/（kg·d） FMN 0.6 mL，末次给药24 h后，处死小鼠并测定小鼠脾脏和胸腺指数；采用酶联免疫吸附试验（ELISA）法测定小肠黏膜组织匀浆中白细胞介素-2（IL-2）、白细胞介素-6（IL-6）及分泌型免疫球蛋白A（sIgA）的含量；常规石蜡包埋组织切片，苏木精-伊红（HE）染色法与过碘酸雪夫（PAS）染色法测定小肠绒毛高度、隐窝深度、绒腺比、上皮内淋巴细胞（IELs）数量以及杯状细胞数量；透射电子显微镜观察小肠黏膜超微结构特性。结果表明:CTX成功复制了小鼠免疫抑制模型，且免疫抑制小鼠小肠黏膜免疫屏障明显受损。FMN各剂量组均可以提高免疫抑制小鼠肠黏膜免疫功能，其中以FMN中剂量组作用最明显，与免疫抑制模型组相比，胸腺指数、小肠黏膜IL-2、IL-6含量差异显著（P<0.05），脾脏指数和肠黏膜sIgA含量差异极显著（P<0.01）。同时，FMN能不同程度地修复免疫抑制小鼠小肠绒毛结构，使小肠绒毛高度和绒腺比增加。电镜观察显示，FMN能不同程度地修复免疫抑制小鼠小肠黏膜上皮细胞的超微结构损伤，维持上皮细胞完整性和连续性。综上所述，FMN可显著改善和促进免疫抑制小鼠小肠黏膜免疫功能的恢复和增强，同时，还可减少小肠黏膜损伤，恢复和促进小肠黏膜结构和功能的稳定。

关键词： 芒柄花素; 小鼠; 免疫抑制; 小肠黏膜; 黏膜免疫

本文引用格式

毛甜甜 , 白衡 , 贾宁 . 芒柄花素对免疫抑制小鼠小肠黏膜免疫功能的影响[J]. 动物营养学报, 2022 , 34(4) : 2712 -2720 . DOI: 10.3969/j.issn.1006-267x.2022.04.064

Abstract

In order to reveal the effects of formonetin (FMN) on small intestinal mucosal immune function of immunosuppressed mice. A total of 50 Kunming mice were randomly divided into five groups:normal control group, immunosuppressive model group, and FMN low dose group, medium dose group and high dose group, with 10 mice in each group, and using intragastric administration of medicine to mice. The experiment lasted for 28 days. In the first 7 days, the mice in normal group were given 0.6 mL physiological saline, and the mice in other groups were given 40 mg/(kg·d) cyclophosphamide (CTX) 0.6 mL. In the next 21 days, the mice in normal group and immunosuppressive model group were given 0.6 mL physiological saline, and the mice in the low dose group, medium dose group and high dose group were given 50, 150, and 250 mg/(kg·d) FMN 0.6 mL, respectively. The 24 h after final administration, the mice were killed, the spleen and thymus indexes were detected. The contents of interleukin-2 (IL-2), interleukin-6 (IL-6) and secretory immunoglobulin A (sIgA) in small intestinal mucosal were detected by enzyme linked immunosorbent assay (ELISA). The morphological structure and intraepithelial lymphocytes (IELs), goblet cells of small intestinal mucosa were investigated by conventional paraffin sectioning technique and histochemicaltechique[hematoxylin-eosin (HE) staining and periodate schiff (PAS) staining]. At the same time, the ultrastructural characteristics of small intestinal mucosa epithelium were analyzed by electron microscope technique. The results showed that the immunosuppressed mice were copied successfully with CTX, and the damage of small intestinal mucosal barrier were obviously. All the dose groups of FMN could improve the intestinal mucosal immune function of immunosuppressed mice, among which the effects of the middle dose group of FMN was the most obvious. Compared with the immunosuppressive group, the thymus index, small intestinal mucosal IL-2 and IL-6 contents were significantly different (P<0.05), while the spleen index and intestinal mucosal sIgA contents were extremely different (P<0.01). Meanwhile, FMN could repair the damage of the small intestinal mucosal and increase the villous height and the ratio of villous height to crypt depth. In the electron microscope, FMN could repair the ultrastructural damage and maintain normal structure of the epithelial cells of small intestinal mucosa in immunosuppressed mice. The results suggest that, FMN can significantly improve and promote the small intestinal mucosal immune function in immunosuppressed mice, at the same time, it can also reduce the structural damage of the small intestinal mucosal and enhance their function.

Key words： formonetin; mice; immunosuppression; small intestinal mucosa; mucosal immunity

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