研究论文 RESEARCH PAPER

脯氨酸对浅色黄姑鱼鱼鳔胶原蛋白沉积及相关基因表达的影响

  • 荣华 ,
  • 杨钰香 ,
  • 王晓雯 ,
  • 严晖 ,
  • 高宇 ,
  • 毕保良 ,
  • 史庆超 ,
  • 孔令富
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  • 1. 云南农业大学动物科学技术学院, 昆明 650201;
    2. 云南省高校高原渔业资源保护与可持续利用重点实验室, 昆明 650201;
    3. 内江师范学院, 长江上游鱼类资源保护与利用 四川省重点实验室, 内江 641112
荣华(1986—),男,湖北荆州人,实验师,博士,主要从事水产动物营养研究。E-mail:15hrong@stu.edu.cn

收稿日期: 2021-10-12

  网络出版日期: 2022-05-14

基金资助

云南省农业基础研究联合专项面上项目(2018FG001-044);长江上游鱼类资源保护与利用四川省重点实验室开发基金项目(NJTCCJSYSYS12);四川省科技厅重点研发项目(2019ZYZF0099)

Effects of Proline on Collagen Deposition and Related Gene Expression in Swim Bladder of Chu's Croaker (Nibea coibor)

  • RONG Hua ,
  • YANG Yuxiang ,
  • WANG Xiaowen ,
  • YAN Hui ,
  • GAO Yu ,
  • BI Baoliang ,
  • SHI Qingchao ,
  • KONG Lingfu
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  • 1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
    2. Key Laboratory of Protection and Sustainable Utilization of Plateau Fishery Resources in University of Yunnan Province, Kunming 650201, China;
    3. Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641112, China

Received date: 2021-10-12

  Online published: 2022-05-14

摘要

鱼鳔富含胶原蛋白,而脯氨酸(Pro)是胶原蛋白合成所需的底物。本试验旨在研究饲料中添加不同水平Pro对浅色黄姑鱼生长、胶原蛋白沉积及相关基因表达的影响。试验选用450尾初始体重为(8.64±0.14) g的浅色黄姑鱼,随机分为6个组,分别投喂添加0、5、10、15、20和25 g/kg Pro的等氮等脂饲料,每组3个重复(网箱),每个重复25尾鱼。试验期8周。试验结束后,测定浅色黄姑鱼的生长性能、各组织中的胶原蛋白及氨基酸含量以及与胶原蛋白代谢相关的基因相对表达量。结果表明:饲料中添加不同水平Pro对浅色黄姑鱼生长性能没有显著影响(P>0.05),但能显著促进鱼鳔中胶原蛋白沉积(P<0.05),同时显著影响鱼鳔中部分氨基酸含量(P<0.05),特别是与胶原蛋白代谢相关的氨基酸。通过鱼鳔胶原蛋白含量估算出饲料中Pro最适添加水平为14.88 g/kg。另外,鱼鳔中Ⅰ型胶原蛋白α1链(COL1A1)、Ⅰ型胶原蛋白α2链(COL1A2)和脯氨酰4-羟化酶亚基α1(P4HA1)基因的相对表达量随着饲料中Pro添加水平的提高而提高(P<0.05)。综上所述,饲料中添加Pro促进了浅色黄姑鱼鱼鳔胶原蛋白的沉积,最佳的添加水平为14.88 g/kg,且Pro促进鱼鳔胶原蛋白沉积潜在受到Ⅰ型胶原蛋白α链基因的调控。

本文引用格式

荣华 , 杨钰香 , 王晓雯 , 严晖 , 高宇 , 毕保良 , 史庆超 , 孔令富 . 脯氨酸对浅色黄姑鱼鱼鳔胶原蛋白沉积及相关基因表达的影响[J]. 动物营养学报, 2022 , 34(5) : 3184 -3193 . DOI: 10.3969/j.issn.1006-267x.2022.05.045

Abstract

Swim bladder was rich in collagen, and proline (Pro) was a substrate required for collagen synthesis. This experiment was designed to study the effects of different supplemental levels of Pro in diets on growth, collagen deposition and related gene expression of Nibea coibor. A total of 450 Nibea coibor with an initial body weight of (8.64±0.14) g were randomly divided into 6 groups with 3 replicates (cages) in each group and 25 fish in each replicate, and each group was fed isonitrogenous and isolipid diets supplemented with 0, 5, 10, 15, 20 and 25 g/kg Pro, respectively. The experiment lasted for 8 weeks. After the experiment, the growth performance, collagen and amino acid contents in each tissue and relative expression levels of genes related to collagen metabolism of Nibea coibor were determined. The results showed that different supplemental levels of Pro in diets had no significant effects on growth performance of Nibea coibor (P>0.05), but significantly promoted the collagen deposition in swim bladder (P<0.05), and significantly affected the contents of some amino acids in swim bladder (P<0.05), especially the amino acids related to collagen metabolism. The optimal supplemental level of Pro in the diet was estimated to be 14.88 g/kg based on the collagen content in swim bladder. In addition, the relative expression levels of collagen type Ⅰ alpha 1 chain (COL1A1), collagen type Ⅰ alpha 2 chain (COL1A2) and prolyl 4-hydroxylase subunit alpha-1 (P4HA1) genes in swim bladder were increased with the increase of dietary Pro supplemental level (P<0.05). In conclusion, dietary Pro can promote collagen deposition in the swim bladder of Nibea coibor, which is potentially regulated by collagen type Ⅰ alpha chain genes. and the optimal supplemental level is 14.88 g/kg.

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