研究论文 RESEARCH PAPER

β-胡萝卜素对青年母兔生长性能、抗氧化能力、生殖激素指标及卵泡发育的影响

  • 孙磊 ,
  • 刘艳华 ,
  • 王志刚 ,
  • 陈宝江
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  • 1. 河北农业大学动物科技学院, 保定 071000;
    2. 河北省牛羊胚胎技术创新中心, 保定 071000
孙磊(1996—),女,河北承德人,硕士研究生,研究方向为动物繁殖营养调控。E-mail:1587738675@qq.com

收稿日期: 2021-11-04

  网络出版日期: 2022-05-14

基金资助

国家兔产业技术体系(CARS-43-B-2);河北省创新能力提升计划项目(20536603D)

Effects of β-Carotene on Growth Performance, Antioxidant Capacity, Reproductive Hormone Indexes and Follicular Development of Young Female Rabbits

  • SUN Lei ,
  • LIU Yanhua ,
  • WANG Zhigang ,
  • CHEN Baojiang
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  • 1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;
    2. Cattle and Sheep Embryo Technology Innovation Center of Hebei Province, Baoding 071000, China

Received date: 2021-11-04

  Online published: 2022-05-14

摘要

本试验旨在研究β-胡萝卜素对青年母兔生长性能、抗氧化能力、生殖激素指标及卵泡发育的影响。选取55~60日龄、体重[(1.679±0.114) kg]相近、健康状态良好的雌性伊拉肉兔160只,随机分为4组,每组20个重复,每个重复2只。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加15(Ⅰ组)、30(Ⅱ组)、45 mg/kg (Ⅲ组)β-胡萝卜素。预试期7 d,正试期28 d。结果表明:1)饲粮中添加β-胡萝卜素对青年母兔终末体重和平均日增重均无显著影响(P>0.05)。2)试验第14天时,与对照组相比,Ⅰ、Ⅱ、Ⅲ组血清谷胱甘肽过氧化物酶(GSH-Px)活性显著升高(P<0.05),Ⅱ、Ⅲ组血清超氧化物歧化酶(SOD)活性显著升高(P<0.05)。试验第28天时,与对照组相比,Ⅰ组血清GSH-Px活性显著升高(P<0.05),Ⅱ、Ⅲ组血清SOD活性显著降低(P<0.05)。3)试验第14天时,与对照组相比,Ⅰ、Ⅱ、Ⅲ组血清促黄体素(LH)含量显著升高(P<0.05),Ⅰ、Ⅱ组血清促卵泡素(FSH)含量显著提高(P<0.05),Ⅰ、Ⅲ组血清雌二醇(E2)和孕酮(PROG)含量显著提高(P<0.05)。试验第28天时,与对照组相比,Ⅰ组血清E2含量显著提高(P<0.05)。4)与对照组相比,Ⅰ、Ⅱ、Ⅲ组卵巢中原始卵泡比例显著降低(P<0.05),Ⅱ、Ⅲ组卵巢中初级卵泡比例显著提高(P<0.05),Ⅰ、Ⅲ组卵巢中次级卵泡和成熟卵泡比例显著提高(P<0.05),Ⅰ、Ⅱ、Ⅲ组卵巢中黄体比例显著提高(P<0.05)。综上所述,饲粮中添加β-胡萝卜素可以改善青年母兔的抗氧化能力,促进卵泡发育和相关激素分泌。本试验条件下,青年母兔饲粮中β-胡萝卜素适宜添加水平为30 mg/kg。

本文引用格式

孙磊 , 刘艳华 , 王志刚 , 陈宝江 . β-胡萝卜素对青年母兔生长性能、抗氧化能力、生殖激素指标及卵泡发育的影响[J]. 动物营养学报, 2022 , 34(5) : 3241 -3250 . DOI: 10.3969/j.issn.1006-267x.2022.05.050

Abstract

This experiment was conducted to investigate the effects of β-carotene on growth performance, antioxidant capacity, reproductive hormone indexes and follicular development of young female rabbits. A total of 160 healthy female Irah meat rabbits aged from 55 to 60 days of age with similar body weight[(1.679±0.114) kg] were randomly divided into 4 groups with 20 replicates per group and 2 rabbits per replicate. Rabbits in the control group were fed a basal diet, and other in experimental groups were fed the basal diet supplemented with 15 (group Ⅰ), 30 (group Ⅱ) and 45 mg/kg (group Ⅲ) β-carotene, respectively. The pretrial period lasted for 7 days and formal period lasted for 28 days. The results showed as follows:1) dietary β-carotene had no significant effects on final body weight and average daily gain of young female rabbits (P>0.05). 2) On day 14 of the experiment, compared with the control group, the serum glutathione peroxidase (GSH-Px) activity of groups Ⅰ, Ⅱ and Ⅲ was significantly increased (P<0.05), and the serum superoxide dismutase (SOD) activity of groups Ⅱ and Ⅲ was significantly increased (P<0.05). On day 28 of the experiment, compared with the control group, the serum GSH-Px activity of group Ⅰ was significantly increased (P<0.05), and the serum SOD activity of groups Ⅱ and Ⅲ was significantly decreased (P<0.05). 3) On day 14 of the experiment, compared with the control group, the serum luteinizing hormone (LH) content of groups Ⅰ, Ⅱ and Ⅲ was significantly increased (P<0.05), the serum follicle stimulating hormone (FSH) content of groups Ⅰ and Ⅱ was significantly increased (P<0.05), and the contents of estradiol (E2) and progesterone (PROG) of groups Ⅰ and Ⅲ were significantly increased (P<0.05). On day 28 of the experiment, compared with the control group, the serum E2 content of group Ⅰ was significantly increased (P<0.05). 4) Compared with the control group, the ovarian primordial follicle proportion of groups Ⅰ, Ⅱ and Ⅲ was significantly decreased (P<0.05), the ovarian primary follicle proportion of groups Ⅱ and Ⅲ was significantly increased (P<0.05), the proportions of secondary follicle and mature follicle in ovarian of groups Ⅰ and Ⅲ were significantly increased (P<0.05), and the ovarian corpus luteum proportion of groups Ⅰ, Ⅱ and Ⅲ was significantly increased (P<0.05). In conclusion, dietary β-carotene can improve the antioxidant capacity of young female rabbits, and promote follicular development and secretion of related hormones. Under this experimental condition, the dietary β-carotene optimal supplemental level of young female rabbits is 30 mg/kg.

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