研究论文 RESEARCH PAPER

饲粮半胱氨酸水平对离乳期梅花鹿生长性能、营养物质表观消化率、血清生化指标和抗氧化能力的影响

  • 杨乾龙 ,
  • 张铁涛 ,
  • 王静 ,
  • 陈丽红 ,
  • 王金铭 ,
  • 郭肖兰 ,
  • 李光玉 ,
  • 王凯英
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  • 中国农业科学院特产研究所, 特种经济动物分子生物学国家重点实验室, 长春 130112
杨乾龙(1994—),男,河南夏邑人,硕士研究生,动物营养与饲料科学专业。E-mail:QianlongY@126.com

收稿日期: 2021-10-04

  网络出版日期: 2022-05-14

基金资助

中国农业科学院科技创新工程项目(CAAS-ASTIP-2021-ISAPS);中国农业科学院科技创新工程项目(CAAS-ASTIP-2020-ISAPS);创新工程专项计划(2060302-21-2021101);吉林省科技攻关计划(20170204041NY)

Effects of Dietary Cysteine Level on Growth Performance, Nutrients Apparent Digestibility, Serum Biochemical Indexes and Antioxidant Capacity of Sika Deer during Weaning Period

  • YANG Qianlong ,
  • ZHANG Tietao ,
  • WANG Jing ,
  • CHEN Lihong ,
  • WANG Jinming ,
  • GUO Xiaolan ,
  • LI Guangyu ,
  • WANG Kaiying
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  • State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received date: 2021-10-04

  Online published: 2022-05-14

摘要

本试验旨在研究饲粮半胱氨酸水平对离乳期梅花鹿生长性能、营养物质表观消化率、血清生化指标和抗氧化能力的影响,以确定离乳期梅花鹿饲粮半胱氨酸的适宜水平。采用单因素试验设计,将16只3月龄、平均体重(24.42±3.86) kg的雄性梅花鹿仔鹿随机分为4组,每组4个重复,每个重复1只。对照组(Ⅰ组)饲喂半胱氨酸水平为2.8 g/kg的基础饲粮,试验组分别饲喂半胱氨酸水平为4.8(Ⅱ组,基础饲粮+2.0 g/kg半胱氨酸)、6.8(Ⅲ组,基础饲粮+4.0 g/kg半胱氨酸)和10.8 g/kg (Ⅳ组,基础饲粮+8.0 g/kg半胱氨酸)的试验饲粮。预试期15 d,正试期30 d。结果表明:1)与Ⅰ组相比,Ⅳ组的平均日采食量显著降低(P<0.05)。2)与Ⅰ组相比,Ⅱ组和Ⅳ组的有机物和干物质表观消化率显著提高(P<0.05),Ⅳ组的中性洗涤纤维和酸性洗涤纤维表观消化率显著提高(P<0.05)。3)与Ⅰ组相比,Ⅳ组的血清低密度脂蛋白含量显著提高(P<0.05),Ⅲ组的血清碱性磷酸酶活性显著提高(P<0.05)。4)与Ⅰ组相比,Ⅱ组的血清过氧化氢酶活性显著提高(P<0.05),Ⅱ组、Ⅲ组和Ⅳ组的血清丙二醛含量显著降低(P<0.05),Ⅱ组和Ⅳ组的血清谷胱甘肽过氧化物酶活性显著提高(P<0.05)。由此可见,在本试验条件下,饲粮半胱氨酸水平为4.8 g/kg时可提高离乳期梅花鹿的干物质和有机物表观消化率及抗氧化能力。

本文引用格式

杨乾龙 , 张铁涛 , 王静 , 陈丽红 , 王金铭 , 郭肖兰 , 李光玉 , 王凯英 . 饲粮半胱氨酸水平对离乳期梅花鹿生长性能、营养物质表观消化率、血清生化指标和抗氧化能力的影响[J]. 动物营养学报, 2022 , 34(5) : 3251 -3260 . DOI: 10.3969/j.issn.1006-267x.2022.05.051

Abstract

This experiment was conducted to study the effects of dietary cysteine level on growth performance, nutrients apparent digestibility, serum biochemical indexes and antioxidant capacity of sika deer during weaning period, and to determine the appropriate dietary cysteine level of sika deer during weaning period. A single factor experimental design was adopted, sixteen 3-month-old healthy sika deer with average body weight of (24.42±3.86) kg were divided into 4 groups with 4 replicates per group and 1 deer per replicate. The control group (group Ⅰ) was fed the basal diet with cysteine level of 2.8 g/kg, and the experimental groups were fed experimental diets with cysteine levels of 4.8 (group Ⅱ, basal diet+2.0 g/kg cysteine), 6.8 (group Ⅲ, basal diet+4.0 g/kg cysteine) and 10.8 g/kg (group Ⅳ, basal diet +8.0 g/kg cysteine), respectively. The pre-experimental period lasted for 15 days, and the experimental period lasted for 30 days. The results showed as follows:1) compared with the group Ⅰ, the average daily feed intake of group Ⅳ was significantly decreased (P<0.05). 2) Compared with the group Ⅰ, the apparent digestibility of organic matter and dry matter of group Ⅱ and group Ⅳ was significantly increased (P<0.05), and the apparent digestibility of neutral detergent fiber and acid detergent fiber of group Ⅳ was significantly increased (P<0.05). 3) Compared with the group Ⅰ, the serum low density lipoprotein content of group Ⅳ was significantly increased (P<0.05), and the serum alkaline phosphatase activity of group Ⅲ was significantly increased (P<0.05). 4) Compared with the group Ⅰ, the serum catalase activity of group Ⅱ was significantly increased (P<0.05), the serum malondialdehyde content of group Ⅱ, group Ⅲ and group Ⅳ was significantly decreased (P<0.05), and the serum glutathione peroxidase activity of group Ⅱ and group Ⅳ was significantly increased (P<0.05). In summary, under the conditions of this experiment, when dietary cysteine level is 4.8 g/kg, it can improve the apparent digestibility of dry matter and organic matter and antioxidant capacity of sika deer during weaning period.

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