动物营养学报 >

2022 , Vol. 34 >Issue 5: 3392 - 3400

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.05.063

相关研究 RELATED RESEARCH

阿魏酸溶液浸泡肉鸡种蛋对鸡胚绒毛尿囊膜血管生成及1日龄肉鸡抗氧化性能的影响

  • 赖世雄 ,
  • 郑玉才 ,
  • 李志雄 ,
  • 唐润姿 ,
  • 杨佳 ,
  • 马维梅 ,
  • 饶开晴
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  • 西南民族大学畜牧兽医学院, 成都 610041
赖世雄(1997—),男,四川遂宁人,硕士研究生,研究方向为动物生长调控。E-mail:1176493921@qq.com

收稿日期: 2021-10-08

  网络出版日期: 2022-05-14

基金资助

研究生创新项目（CX2021SZ61）；四川省重点研发项目（2021YFYZ0031）；四川省科技计划项目（2020YFSY0048）

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Effects of Broiler Fertilized Egg Soaked in Ferulic Acid Solution on Chorioallantoie Membrane Angiogenesis of Chicken Embryo and Antioxidant Performance of One-Day-Old Broiler Chickens

  • LAI Shixiong ,
  • ZHENG Yucai ,
  • LI Zhixiong ,
  • TANG Runzi ,
  • YANG Jia ,
  • MA Weimei ,
  • RAO Kaiqing
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  • College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China

Received date: 2021-10-08

  Online published: 2022-05-14

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摘要

本试验旨在研究阿魏酸溶液浸泡肉鸡种蛋对鸡胚绒毛尿囊膜（CAM）血管生成及1日龄肉鸡抗氧化性能的影响，为阿魏酸在禽类养殖中的应用提供一定的理论和试验依据。选取重量[（60±3） g]相近的黄羽肉鸡种蛋132枚，随机分为3组，每组44枚。种蛋孵化至7、8、9胚龄时，低浓度组和高浓度组种蛋每天分别用40和120 mg/L阿魏酸溶液浸泡6 min，对照组种蛋用纯水浸泡相同时间，并放回孵化器中继续孵化。结果表明：1）与对照组相比，高浓度组种蛋出雏率降低了22％。2）与对照组相比，低浓度组鸡胚CAM中血管相对面积和新生血管数目显著升高（P<0.05），而高浓度组鸡胚CAM中血管相对面积和新生血管数目显著降低（P<0.05）。3）与对照组相比，低浓度组鸡胚CAM中血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子2（FGF2）基因表达均显著上调（P<0.05）。4）与对照组相比，高浓度组血清超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性显著升高（P<0.05），血清丙二醛（MDA）含量显著降低（P<0.05）；低浓度组血清GSH-Px活性显著升高（P<0.05）；低浓度组和高浓度组肝脏SOD活性和MDA含量显著降低（P<0.05）。5）与对照组相比，低浓度组和高浓度组肝脏SOD基因表达显著下调（P<0.05）。由此可见，低浓度阿魏酸溶液浸泡种蛋能通过上调鸡胚CAM中VEGF和FGF2基因表达，促进鸡胚CAM血管生成，而高浓度阿魏酸溶液浸泡种蛋会抑制鸡胚CAM血管生成，降低出雏率；低、高浓度阿魏酸溶液浸泡种蛋均能提升1日龄肉鸡的抗氧化性能。

关键词： 阿魏酸; 浸泡法; 血管生成; 抗氧化; 种蛋; 肉鸡

本文引用格式

赖世雄 , 郑玉才 , 李志雄 , 唐润姿 , 杨佳 , 马维梅 , 饶开晴 . 阿魏酸溶液浸泡肉鸡种蛋对鸡胚绒毛尿囊膜血管生成及1日龄肉鸡抗氧化性能的影响[J]. 动物营养学报, 2022 , 34(5) : 3392 -3400 . DOI: 10.3969/j.issn.1006-267x.2022.05.063

Abstract

The purpose of this study was to explore the effects of broiler fertilized egg soaked in ferulic acid solution on chorioallantoie membrane (CAM) angiogenesis of chicken embryo and antioxidant performance of one-day-old broiler chickens, to provide theoretical and experimental basis for the application of ferulic acid in the poultry industry. One hundred and thirty-two yellow chicken fertilized eggs with similar weight[(60±3) g] were divided into 3 groups with 44 eggs per group. When the fertilized eggs were hatched to the 7, 8 and 9 days of embryo, the fertilized eggs in low concentration group and high concentration group were soaked in 40 and 120 mg/L ferulic acid solution for 6 min every day, the fertilized eggs in the control group were soaked in pure water for the same time, and then continued to hatch in the incubator. The results showed as follows:1) compared with the control group, the hatching rate of fertilized eggs of high concentration group decreased by 22%. 2) Compared with the control group, the vascular relative area and number of neovascularization in chicken embryo CAM of low concentration group were significantly increased (P<0.05), while the vascular relative area and number of neovascularization in chicken embryo CAM of high concentration group were significantly decreased (P<0.05). 3) Compared with the control group, the gene expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor 2 (FGF2) in chicken embryo CAM of low concentration group were significantly up-regulated (P<0.05). 4) Compared with the control group, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum of high concentration group were significantly increased (P<0.05), and the serum malondialdehyde (MDA) content was significantly decreased (P<0.05); the serum GSH-Px activity of low concentration group was significantly increased (P<0.05); the liver SOD activity and MDA content of low concentration group and high concentration group were significantly decreased (P<0.05). 5) Compared with the control group, the gene expression of SOD in liver of low concentration group and high concentration group was significantly down-regulated (P<0.05). In conclusion, the fertilized eggs soaked in low concentration ferulic acid solution can promote chicken embryo CAM angiogenesis by up-regulate the gene expression levels of VEGF and FGF2 in chicken embryo CAM, while the fertilized eggs soaked in high concentration ferulic acid solution can inhibit chicken embryo CAM angiogenesis and reduce the hatching rate; the fertilized eggs soaked in low and high concentration ferulic acid solution can improve the antioxidant performance of one-day-old broiler chickens.

Key words： ferulic acid; soaking method; vasculogenesis; antioxidant; fertilized eggs; broiler chickens

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