综述 REVIEW

抗菌肽在动物生产中应用的研究进展

  • 来振衡 ,
  • 陈虹羽 ,
  • 吕银凤 ,
  • 单安山
展开
  • 东北农业大学动物营养研究所, 哈尔滨 150030
来振衡(1995—),男,河南兰考人,博士研究生,研究方向为饲用抗菌肽的设计、优化及生产应用转化。E-mail:B20050112@neau.edu.cn

收稿日期: 2021-12-07

  网络出版日期: 2022-06-14

基金资助

国家自然科学基金项目(32030101,31872368);黑龙江省自然科学基金团队项目(TD2019C001)

Research Progress on Application of Antimicrobial Peptides in Animal Production

  • LAI Zhenheng ,
  • CHEN Hongyu ,
  • LYU Yinfeng ,
  • SHAN Anshan
Expand
  • Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China

Received date: 2021-12-07

  Online published: 2022-06-14

摘要

抗菌肽具有抗菌、抗炎、促生长等功能,与传统抗生素相比,它不易产生耐药性,被认为极具替代饲用抗生素的潜在价值。近年来抗菌肽在动物生产中进行了广泛的应用研究,本文综述了这一领域的研究进展,旨在为饲用抗菌肽的研发与应用提供参考。

本文引用格式

来振衡 , 陈虹羽 , 吕银凤 , 单安山 . 抗菌肽在动物生产中应用的研究进展[J]. 动物营养学报, 2022 , 34(6) : 3401 -3410 . DOI: 10.3969/j.issn.1006-267x.2022.06.001

Abstract

Since antimicrobial peptides with antibacterial, anti-inflammatory, and growth-promoting functions are less prone to induce drug resistance than conventional antibiotics, they are considered as a potential substitute for feed antibiotics. In recent years, antimicrobial peptides have been extensively discussed in animal feed. In this review, we concluded the research progress of antimicrobial peptides in animal production, aiming to provide a reference for the research and development of antimicrobial peptides for feeding.

参考文献

[1] MOOKHERJEE N, ANDERSON M A, HAAGSMAN H P, et al.Antimicrobial host defence peptides:functions and clinical potential[J].Nature Reviews Drug Discovery, 2020, 19(5):311-332.  
[2] SHAO C X, ZHU Y J, JIAN Q, et al.Cross-strand interaction, central bending, and sequence pattern act as biomodulators of simplified β-hairpin antimicrobial amphiphiles[J].Small, 2021, 17(7):2003899.
[3] BOPARAI J K, SHARMA P K.Mini review on antimicrobial peptides, sources, mechanism and recent applications[J].Protein and Peptide Letters, 2020, 27(1):4-16.
[4] LAI Z H, JIAN Q, LI G Y, et al.Self-assembling peptide dendron nanoparticles with high stability and a multimodal antimicrobial mechanism of action[J].ACS Nano, 2021, 15(10):15824-15840.  
[5] FANG Y X, ZHU Y H, LI L, et al.Biomaterial-interrelated bacterial sweeper:simplified self-assembled octapeptides with double-layered Trp zipper induces membrane destabilization and bacterial apoptosis-like death[J].Small Methods, 2021, 5(12):e2101304.
[6] ENGELBERG Y, LANDAU M.The human LL-37(17-29) antimicrobial peptide reveals a functional supramolecular structure[J].Nature Communications, 2020, 11(1):3894.
[7] ZHONG C, ZHANG F Y, YAO J, et al.New antimicrobial peptides with repeating unit against multidrug-resistant bacteria[J].ACS Infectious Diseases, 2021, 7(6):1619-1637.  
[8] PEN G H, YANG N, TENG D, et al.A review on the use of antimicrobial peptides to combat porcine viruses[J].Antibiotics, 2020, 9(11):801.
[9] 郭强, 何涛.妊娠母猪日粮中添加天蚕素抗菌肽对繁殖性能及产后仔猪生长性能的影响[J].饲料与畜牧, 2012(12):54-56. GUO Q, HE T.The effect of adding cecropin antimicrobial peptide in the diet of pregnant sows on reproductive performance and growth performance of postpartum piglets[J].Feed and Husbandry, 2012(12):54-56.(in Chinese)
[10] 陈星星, 江康峰, 尹会方, 等.抗菌肽制剂对妊娠母猪蓝耳病抗体水平及生产性能的影响[J].福建畜牧兽医, 2016, 38(2):9-11. CHEN X X, JIANG K F, YIN H F, et al.Effect of antimicrobial peptide on the levels of the PRRS antibody and production performance of pregnant sows[J].Fujian Journal of Animal Husbandry and Veterinary Medicine, 2016, 38(2):9-11.(in Chinese)
[11] CAMPBELL J M, CRENSHAW J D, POLO J.The biological stress of early weaned piglets[J].Journal of Animal Science and Biotechnology, 2013, 4(1):19.
[12] MOESER A J, POHL C S, RAJPUT M.Weaning stress and gastrointestinal barrier development:implications for lifelong gut health in pigs[J].Animal Nutrition, 2017, 3(4):313-321.  
[13] KARASOVA D, CRHANOVA M, BABAK V, et al.Development of piglet gut microbiota at the time of weaning influences development of postweaning diarrhea-a field study[J].Research in Veterinary Science, 2021, 135:59-65.
[14] FENG J S, WANG L, XIE Y S, et al.Effects of antimicrobial peptide cathelicidin-BF on diarrhea controlling, immune responses, intestinal inflammation and intestinal barrier function in piglets with postweaning diarrhea[J].International Immunopharmacology, 2020, 85:106658.
[15] VANROLLEGHEM W, TANGHE S, VERSTRINGE S, et al.Potential dietary feed additives with antibacterial effects and their impact on performance of weaned piglets:a meta-analysis[J].The Veterinary Journal, 2019, 249:24-32.
[16] HU W Y, YANG Y Y, LI Z, et al.Antibacterial, cytotoxicity and mechanism of the antimicrobial peptide KR-32 in weaning piglets[J].International Journal of Peptide Research and Therapeutics, 2020, 26(2):943-953.  
[17] PENG Z X, WANG A R, XIE L Q, et al.Use of recombinant porcine β-defensin 2 as a medicated feed additive for weaned piglets[J].Scientific Reports, 2016, 6:26790.
[18] 卜艳玲, 陈静, 李建涛, 等.饲粮中添加肠杆菌肽对断奶仔猪生产性能和血清生化指标的影响[J].动物营养学报, 2018, 30(2):696-706. BU Y L, CHEN J, LI J T, et al.Effects of enterobacitracin supplementation on performance and serum biochemical indices of weaning piglets[J].Chinese Journal of Animal Nutrition, 2018, 30(2):696-706.(in Chinese)
[19] REN Z H, YUAN W, DENG H D, et al.Effects of antibacterial peptide on cellular immunity in weaned piglets[J].Journal of Animal Science, 2015, 93(1):127-134.  
[20] TANG Z R, XU L, SHI B S, et al.Oral administration of synthetic porcine beta-defensin-2 improves growth performance and cecal microbial flora and down-regulates the expression of intestinal toll-like receptor-4 and inflammatory cytokines in weaned piglets challenged with enterotoxigenic Escherichia coli[J].Animal Science Journal, 2016, 87(10):1258-1266.  
[21] ZHANG H W, ZHANG B X, ZHANG X M, et al.Effects of cathelicidin-derived peptide from reptiles on lipopolysaccharide-induced intestinal inflammation in weaned piglets[J].Veterinary Immunology and Immunopathology, 2017, 192:41-53.
[22] YU H T, DING X L, LI N, et al.Dietary supplemented antimicrobial peptide microcin J25 improves the growth performance, apparent total tract digestibility, fecal microbiota, and intestinal barrier function of weaned pigs[J].Journal of Animal Science, 2017, 95(11):5064-5076.  
[23] CAO C Y, LI J N, MA Q Y, et al.Effects of dietary supplementation with the antimicrobial peptide WK3 on growth performance and intestinal health in diarrheic weanling piglets[J].Journal of Applied Animal Research, 2021, 49(1):147-153.  
[24] ZHANG L C, GUO T, ZHAN N, et al.Effects of the antimicrobial peptide WK3 on diarrhea, growth performance and intestinal health of weaned piglets challenged with enterotoxigenic Escherichia coli K88[J].Food & Nutrition Research, 2021, 65:3448.
[25] 邓柏林, 何宏轩, 张乃锋, 等.日粮中添加抗菌肽对育肥猪消化率及其对氮排放影响的试验研究[J].猪业科学, 2013, 30(3):40-42. DENG B L, HE H X, ZHANG N F, et al.Experimental study on the effect of adding antimicrobial peptides to the diet on the digestibility of fattening pigs and its effect on nitrogen emissions[J].Swine Industry Science, 2013, 30(3):40-42.(in Chinese)
[26] 侯改凤, 李瑞, 韦良开, 等.抗菌肽对育肥猪生长性能及血液生理生化指标的影响[J].中国饲料, 2017(12):24-26, 44. HOU G F, LI R, WEI L K, et al.Effects of antibacterical peptides on growth performance and blood physiological and biochemical indexes in finishing pigs[J].China Feed, 2017(12):24-26, 44.(in Chinese)
[27] 张彬, 陶恒勋, 赵自力, 等.抗菌肽制剂对育肥猪生长性能的影响[J].养殖与饲料, 2013(9):21-23. ZHANG B, TAO H X, ZHAO Z L, et al.The effect of antimicrobial peptide preparations on the growth performance of fattening pigs[J].Animals Breeding and Feed, 2013(9):21-23.(in Chinese)
[28] 李瑞, 韦良开, 侯改凤, 等.饲粮添加抗菌肽制剂对肥育猪胴体性状及肉品质的影响[J].养猪, 2018(3):49-51. LI R, WEI L K, HOU G F, et al.Effects of supplementing diet with antimicrobial peptide on carcass and meat quality of finishing pigs[J].Swine Production, 2018(3):49-51.(in Chinese)
[29] YESTE M.Recent advances in boar sperm cryopreservation:state of the art and current perspectives[J].Reproduction in Domestic Animals, 2015, 50(Suppl.2):71-79.
[30] PUIG-TIMONET A, CASTILLO-MARTÍN M, PEREIRA B A, et al.Evaluation of porcine beta defensins-1 and -2 as antimicrobial peptides for liquid-stored boar semen:effects on bacterial growth and sperm quality[J].Theriogenology, 2018, 111:9-18.
[31] BUSSALLEU E, SANCHO S, BRIZ M D, et al.Do antimicrobial peptides PR-39, PMAP-36 and PMAP-37 have any effect on bacterial growth and quality of liquid-stored boar semen?[J].Theriogenology, 2017, 89:235-243.
[32] SCHULZE M, DATHE M, WABERSKI D, et al.Liquid storage of boar semen:current and future perspectives on the use of cationic antimicrobial peptides to replace antibiotics in semen extenders[J].Theriogenology, 2016, 85(1):39-46.  
[33] MA J L, ZHAO L H, SUN D D, et al.Effects of dietary supplementation of recombinant plectasin on growth performance, intestinal health and innate immunity response in broilers[J].Probiotics and Antimicrobial Proteins, 2020, 12(1):214-223.  
[34] TAI H M, YOU M F, LIN C H, et al.Scale-up production of and dietary supplementation with the recombinant antimicrobial peptide tilapia piscidin 4 to improve growth performance in Gallus gallus domesticus[J].PLoS One, 2021, 16(6):e0253661.
[35] 马倩, 赵衍铜, 柏明娜, 等.抗菌肽对芦花鸡生产性能和血清生化指标的影响[J].饲料研究, 2012(3):27-29. MA Q, ZHAO Y T, BAI M N, et al.Effects of antimicrobial peptides on the production performance and serum biochemical indexes of reed chicken[J].Feed Research, 2012(3):27-29.(in Chinese)
[36] 董丽娜, 姜宁, 张爱忠, 等.含抗菌肽酵母工程菌对肉仔鸡生长性能、营养物质代谢率和血液生化指标的影响[J].黑龙江畜牧兽医, 2018(5):185-190. DONG L N, JIANG N, ZHANG A Z, et al.Effects of yeast engineering bacteria containing antimicrobial peptides on growth performance, metabolic rate of nutrients and blood biochemical indices of broilers[J].Heilongjiang Animal Science and Veterinary Medicine, 2018(5):185-190.(in Chinese)
[37] 宋琼莉, 陈小连, 周泉勇, 等.抗菌肽及益生菌复合添加剂对青脚麻鸡生长性能、屠宰性能及血清生化指标的影响[J].中国畜牧兽医, 2018, 45(3):690-697. SONG Q L, CHEN X L, ZHOU Q Y, et al.Effects of composite additives of antimicrobial peptides and probiotics on growth performance, slaughter performance and serum biochemical indexes in partridge shank chickens[J].China Animal Husbandry & Veterinary Medicine, 2018, 45(3):690-697.(in Chinese)
[38] 郭忠欣, 王天奇.抗菌肽对肉鸡生长性能、屠宰性能、肉品质和免疫功能的影响[J]. 饲料研究, 2021, 44(8):37-40. GUO Z X, WANG T Q. Effect of antimicrobial peptides on growth performance, slaughter performance, meat quality and immune function of broilers[J].Feed Research, 2021, 44(8):37-40.(in Chinese)
[39] 王棚, 曹原, 铁鲲源, 等.抗菌肽粗提物对产蛋后期鸡产蛋性能、蛋品质、脏器指数、血清生化指标及免疫功能的影响[J].中国兽医学报, 2018, 38(4):819-823. WANG P, CAO Y, TIE K Y, et al.Effect of crude extract of antimicrobial peptide on production performance, egg quality, viscera index, serum biochemical indexes and immune function of late laying hens[J].Chinese Journal of Veterinary Science, 2018, 38(4):819-823.(in Chinese)
[40] XIE Z, ZHAO Q Q, WANG H, et al.Effects of antibacterial peptide combinations on growth performance, intestinal health, and immune function of broiler chickens[J].Poultry Science, 2020, 99(12):6481-6492.  
[41] 孙全友, 彭翔, 李杰, 等.姜黄素和抗菌肽对肉仔鸡生长性能和免疫机能的影响及其互作效应研究[J].中国畜牧杂志, 2014, 50(17):62-67. SUN Q Y, PENG X, LI J, et al.Study on effects of curcumin and antimicrobial peptide on growth performance and immune function of broilers[J].Chinese Journal of Animal Science, 2014, 50(17):62-67.(in Chinese)
[42] BAI J, WANG R, YAN L, et al.Co-supplementation of dietary seaweed powder and antibacterial peptides improves broiler growth performance and immune function[J].Brazilian Journal of Poultry Science, 2019, 21(2):1-9.
[43] MOHAMMADREZAEI M, NAVIDSHAD B, GHEISARI A, et al.Cottonseed meal bioactive peptides as an alternative to antibiotic growth promoters in broiler chicks[J].International Journal of Peptide Research and Therapeutics, 2021, 27(1):329-340.  
[44] 董丽娜, 姜宁, 张萌萌, 等.含抗菌肽酵母工程菌对肉仔鸡肠道菌群和免疫功能的影响[J].中国微生态学杂志, 2018, 30(3):254-259. DONG L N, JIANG N, ZHANG M M, et al.Effects of yeast engineering bacteria containing antimicrobial peptides Cec Md on intestinal flora and immune function of broilers[J].Chinese Journal of Microecology, 2018, 30(3):254-259.(in Chinese)
[45] SHOLIKIN M M, SADARMAN S, IRAWAN I, et al.Antimicrobial peptides as an additive in broiler chicken nutrition:a Meta-analysis of bird performance, nutrient digestibility and serum metabolites[J].Journal of Animal and Feed Sciences, 2021, 30(2):100-110.  
[46] HU F, GAO X, SHE R, et al.Effects of antimicrobial peptides on growth performance and small intestinal function in broilers under chronic heat stress[J].Poultry Science, 2017, 96(4):798-806.  
[47] KO S K K, PARASO M G V, PAJAS A M G A, et al.Immunomodulatory responses in plectasin-supplemented broilers under tropical environmental conditions[J].Tropical Animal Health and Production, 2021, 53(2):253.
[48] 钟宏鹏, 刘艳环, 朱言柱, 等.MSL抗菌肽对鸡白痢的预防作用[J].特产研究, 2013, 35(3):23-26. ZHONG H P, LIU Y H, ZHU Y Z, et al.Protective role of MSL on the pullorum disease[J].Special Wild Economic Animal and Plant Research, 2013, 35(3):23-26.(in Chinese)
[49] 郭文洁, 高锋, 吕磊, 等.腹泻鸡粪培养蝇蛆抗菌肽对患病鸡疗效的研究[J].黑龙江畜牧兽医, 2017(18):154-156. GUO W J, GAO F, LV L, et al.Study on the curative effect of myiasis antimicrobial peptides from chicken manure with diarrhea on diseased chickens[J].Heilongjiang Animal Science and Veterinary Medicine, 2017(18):154-156.(in Chinese)
[50] WICKRAMASURIYA S S, PARK I, LEE Y, et al.Oral delivery of Bacillus subtilis expressing chicken NK-2 peptide protects against Eimeria acervulina infection in broiler chickens[J].Frontiers in Veterinary Science, 2021, 8:684818.
[51] DANESHMAND A, KERMANSHAHI H, SEKHAVATI M H, et al.Antimicrobial peptide, cLF36, affects performance and intestinal morphology, microflora, junctional proteins, and immune cells in broilers challenged with E. coli[J].Scientific Reports, 2019, 9(1):14176.
[52] DANESHMAND A, KERMANSHAHI H, SEKHAVATI M H, et al.Effects of cLFchimera peptide on intestinal morphology, integrity, microbiota, and immune cells in broiler chickens challenged with necrotic enteritis[J].Scientific Reports, 2020, 10(1):17704.
[53] 陈晓生, 张辉华, 田允波, 等.抗菌肽作饲料添加剂对肉鸭生长性能的影响[J].黑龙江畜牧兽医, 2005(3):64-65. CHEN X S, ZHANG H H, TIAN Y B, et al.Effects of antimicrobial peptides as feed additives on the growth performance of meat ducks[J].Heilongjiang Animal Science and Veterinary Medicine, 2005(3):64-65.(in Chinese)
[54] 陈晓生, 张辉华, 周庆国, 等.抗菌肽对肉鸭雏鸭期肠道主要微生物菌落的影响[J].兽药与饲料添加剂, 2006(4):1-2. CHEN X S, ZHANG H H, ZHOU Q G, et al.Effects of antimicrobial peptides on the main microbial colonies in the intestines of meat ducks[J].Veterinary Pharmaceuticals & Feed Additives, 2006(4):1-2.(in Chinese)
[55] 陈晓生, 刘为民, 周庆国, 等.饲粮中添加抗菌肽对肉鸭血清代谢激素及生理生化指标的影响[J].兽药与饲料添加剂, 2005, 10(2):4-6. CHEN X S, LIU W M, ZHOU Q G, et al.Effects of adding antimicrobial peptides in diets on serum metabolic hormones and physiological and biochemical indexes of meat ducks[J].Veterinary Pharmaceuticals & Feed Additives, 2005, 10(2):4-6.(in Chinese)
[56] 杨颜铱, 邓俊良, 陈芸, 等.精料水平和复合抗菌肽对川中黑山羊生长性能及血清中免疫球蛋白、补体、细胞因子和激素水平的影响[J].浙江农业学报, 2017, 29(8):1243-1252. YANG Y Y, DENG J L, CHEN Y, et al.Effects of concentrate level and dietary supplementation of recombinant antimicrobial peptides(AMPs) on growth performance and serum immunoglobulin, cytokines, complement, hormone levels in male Chuanzhong black goats[J].Acta Agriculturae Zhejiangensis, 2017, 29(8):1243-1252.(in Chinese)
[57] 刘旗, 陈芸, 邓俊良, 等.复合抗菌肽对川中黑山羊瘤胃纤毛虫种群结构的影响[J].农业生物技术学报, 2017, 25(10):1689-1696. LIU Q, CHEN Y, DENG J L, et al.Effects of antibacterial peptides on rumen ciliate (Rumen ciliata) community structure in Chuanzhong black goat (Capra hircus)[J].Journal of Agricultural Biotechnology, 2017, 25(10):1689-1696.(in Chinese)
[58] LIU Q, YAO S H, CHEN Y, et al.Use of antimicrobial peptides as a feed additive for juvenile goats[J].Scientific Reports, 2017, 7(1):12254.
[59] DYAR O J, ZHANG T Y, PENG Y, et al.Knowledge, attitudes and practices relating to antibiotic use and antibiotic resistance among backyard pig farmers in rural Shandong province, China[J].Preventive Veterinary Medicine, 2020, 175:104858.
[60] YANG H, PARUCH L, CHEN X J, et al.Antibiotic application and resistance in swine production in China:current situation and future perspectives[J].Frontiers in Veterinary Science, 2019, 6:136.
[61] OKORIE-KANU O J, ANYANWU M U, EZENDUKA E V, et al.Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers[J].PLoS One, 2020, 15(5):e0232913.
[62] HE Y, YUAN Q B, MATHIEU J, et al.Antibiotic resistance genes from livestock waste:occurrence, dissemination, and treatment[J].NPJ Clean Water, 2020, 3(1):4.
[63] SURIYAPHOL P, CHIU J K H, YIMPRING N, et al.Dynamics of the fecal microbiome and antimicrobial resistome in commercial piglets during the weaning period[J].Scientific Reports, 2021, 11(1):18091.
[64] VERKOLA M, PIETOLA E, JÄRVINEN A, et al.Low prevalence of zoonotic multidrug-resistant bacteria in veterinarians in a country with prudent use of antimicrobials in animals[J].Zoonoses and Public Health, 2019, 66(6):667-678.  
[65] SILVEIRA R F, ROQUE-BORDA C A, VICENTE E F.Antimicrobial peptides as a feed additive alternative to animal production, food safety and public health implications:an overview[J].Animal Nutrition, 2021, 7(3):896-904.  
文章导航

/