研究论文 RESEARCHPAPER

7种中草药对肉牛瘤胃体外发酵及甲烷产量的影响

  • 丁子悦 ,
  • 薛白 ,
  • 蔡凌云 ,
  • 任伊凡 ,
  • 王之盛 ,
  • 王立志 ,
  • 彭全辉 ,
  • 胡瑞 ,
  • 邹华围
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  • 1. 四川农业大学动物营养研究所, 四川省牛低碳养殖与安全生产高校重点实验室, 成都 611130;
    2. 凯里学院大健康学院, 凯里 556011
丁子悦(1997—),女,山西临汾人,硕士研究生,从事反刍动物营养研究。E-mail:ding_ziyue@163.com

收稿日期: 2021-11-22

  网络出版日期: 2022-06-14

基金资助

贵州省科技厅项目(黔科合J字[2013]2258号)

Effects of 7 Kinds of Chinese Herbs on in Vitro Rumen Fermentation and Methane Production of Beef Cattle

  • DING Ziyue ,
  • XUE Bai ,
  • CAI Lingyun ,
  • REN Yifan ,
  • WANG Zhisheng ,
  • WANG Lizhi ,
  • PENG Quanhui ,
  • HU Rui ,
  • ZOU Huawei
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  • 1. Key Laboratory of University in Cattle Low Carbon Breeding and Safety Production in Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China;
    2. School of Life and Health Science, Kaili University, Kaili 556011, China

Received date: 2021-11-22

  Online published: 2022-06-14

摘要

本试验旨在利用体外发酵法探讨7种中草药及其不同添加剂量对肉牛瘤胃发酵及甲烷(CH4)等温室气体产量的影响。以3头体况相近的健康筠连黄牛作为瘤胃液供体,采用7×3+1两因素完全随机试验设计,设置7种中草药(野葛根、荆芥、柴胡、金银花、钩藤、仙鹤草和五香血藤)和3种添加剂量(分别为每200 mg底物额外添加15、20、25 mg),并设置1个不添加中草药的对照,共计22个处理,每个处理设置3个重复。测定体外发酵24 h后的产气量(GP)、瘤胃发酵参数以及CH4、二氧化碳(CO2)和氢气(H2)产量。结果表明:1)中草药种类对GP,氨态氮(NH3-N)、微生物蛋白(MCP)、总挥发性脂肪酸(TVFA)、乙酸、丙酸、丁酸浓度,乙酸/丙酸值以及H2、CH4和CO2产量均有极显著影响(P<0.01),添加剂量对NH3-N、MCP、TVFA及丙酸浓度有显著或极显著影响(P<0.05或P<0.01),中草药种类与添加剂量的交互效应对NH3-N、TVFA、乙酸和丁酸浓度有显著影响(P<0.05)。2)与不添加中草药的对照相比,添加25 mg野葛根显著提高了TVFA、乙酸、丙酸浓度以及H2产量(P<0.05);添加3种剂量的荆芥均显著提高了丁酸浓度(P<0.05),添加20 mg荆芥还使NH3-N、TVFA、乙酸浓度及H2、CH4产量显著增加(P<0.05);添加3种剂量的金银花均显著降低了乙酸/丙酸值(P<0.05),添加20 mg金银花还显著降低了CH4和CO2产量(P<0.05);添加25 mg仙鹤草显著提高了MCP浓度(P<0.05),且使CO2产量显著降低(P<0.05);添加3种剂量的五香血藤均显著降低了乙酸/丙酸值(P<0.05),添加15、20 mg五香血藤还使NH3-N、TVFA和乙酸浓度显著降低(P<0.05);添加3种剂量的柴胡和钩藤对各指标均无显著影响(P>0.05)。综上所述,金银花和仙鹤草具有降低体外发酵温室气体产量的作用,且不会对瘤胃发酵造成不良影响。

本文引用格式

丁子悦 , 薛白 , 蔡凌云 , 任伊凡 , 王之盛 , 王立志 , 彭全辉 , 胡瑞 , 邹华围 . 7种中草药对肉牛瘤胃体外发酵及甲烷产量的影响[J]. 动物营养学报, 2022 , 34(6) : 3710 -3723 . DOI: 10.3969/j.issn.1006-267x.2022.06.032

Abstract

This experiment was aimed to investigate the effects of 7 kinds of Chinese herbs and their additional doses on in vitro rumen fermentation and greenhouse gas like methane (CH4) production of beef cattle. Three healthy Junlian yellow cattle with similar body condition were used as rumen fluid donors. A 7×3+1 completely randomized design was set on with 7 Chinese herbs[Pueraria montana (Lour.) Merr., Nepeta cataria L., Bupleurum Chinense DC., Lonicera japonica Thunb., Uncaria rhynchophylla (Miq.), Agrimonia pilosa Ledeb. and Schisandra sphenanthera Rehder & E. H. Wilson] and 3 additional doses (15, 20 and 25 mg extra per 200 mg substrate), and a control without supplementation of Chinese herb was set to form 22 treatments with 3 replication each. The gas production (GP), rumen fermentation parameters and the production of CH4, carbon dioxide (CO2) and hydrogen (H2) were measured after 24 h in vitro fermentation. The results showed as follows:1) the species of Chinese herb had extreme significant effects on GP, ammonia nitrogen (NH3-N), microbial crude protein (MCP), total volatile fatty acids (TVFA), acetic acid (AA), propionic acid (PA), butyric acid (BA) concentrations, AA/PA (A/P) ratio and H2, CH4 and CO2 production (P<0.01). The additional dose had significant or extreme significant effects on NH3-N, MCP, TVFA and PA concentrations (P<0.05 or P<0.01). The interaction between the species of Chinese herb and the additional dose had significant effects on the concentrations of NH3-N, TVFA, AA and BA (P<0.05). 2) Compared with the control group without supplementation of Chinese herb, the concentrations of TVFA, AA, PA and H2 production were significantly increased by adding 25 mg Pueraria montana (Lour.) Merr. (P<0.05); adding 3 doses of Nepeta cataria L. significantly increased the BA concentration, also the NH3-N, TVFA, AA concentrations and H2, CH4 production were significantly increased by adding 20 mg Nepeta cataria L. (P<0.05); adding 3 doses of Lonicera japonica Thunb. significantly decreased the A/P ratio, else the CH4 and CO2 production were significantly decreased by adding 20 mg Lonicera japonica Thunb. (P<0.05); the concentration of MCP was significantly increased (P<0.05), while the CO2 production was significantly reduced by adding 25 mg Agrimonia pilosa Ledeb. (P<0.05); adding 3 doses of Schisandra sphenanthera significantly decreased the A/P ratio, also the concentrations of NH3-N, TVFA and AA were significantly decreased by adding 15 and 20 mg Schisandra sphenanthera (P<0.05). There were no significant effects on each index when adding 3 doses of Bupleurum Chinense DC. and Uncaria rhynchophylla (Miq.) compared with the control group (P>0.05). Based on the above, Lonicera japonica Thunb. and Agrimonia pilosa Ledeb. have potential for reducing greenhouse gas production of in vitro fermentation, and have no adverse effect on rumen fermentation.

参考文献

[1] 胡红莲, 卢德勋, 高民.反刍动物甲烷的排放及其减排调控技术[J].畜牧与饲料科学, 2011, 32(增刊1):26-29. HU H L, LU D X, GAO M.Methane emission from ruminants and the regulation and control techniques[J].Animal Husbandry and Feed Science, 2011, 32(Suppl.1):26-29.(in Chinese)
[2] ZHAO X W, WANG J Q, YANG Y X, et al.Effects of different fat mixtures on milk fatty acid composition and oxidative stability of milk fat[J].Animal Feed Science and Technology, 2013, 185(1/2):35-42.
[3] ECKARD R J, GRAINGER C, DE KLEIN C A M.Options for the abatement of methane and nitrous oxide from ruminant production:a review[J].Livestock Science, 2010, 130(1/2/3):47-56.
[4] CALLAWAY T R, CARNEIRO DE MELO A M, RUSSELL J B.The effect of nisin and monensin on ruminal fermentations in vitro[J].Current Microbiology, 1997, 35(2):90-96.  
[5] HOOK S E, NORTHWOOD K S, WRIGHT A D G, et al.Long-term monensin supplementation does not significantly affect the quantity or diversity of methanogens in the rumen of the lactating dairy cow[J].Applied and Environmental Microbiology, 2009, 75(2):374-380.  
[6] PATRA A K, SAXENA J.A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen[J].Phytochemistry, 2010, 71(11/12):1198-1222.
[7] BODAS R, LÓPEZ S, FERNÁNDEZ M, et al.In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants[J].Animal Feed Science and Technology, 2008, 145(1/2/3/4):245-258.
[8] GARCÍA-GONZÁLEZ R, LÓPEZ S, FERNÁNDEZ M, et al.Effects of the addition of some medicinal plants on methane production in a rumen simulating fermenter (RUSITEC)[J].International Congress Series, 2006, 1293:172-175.
[9] DURMIC Z, HUTTON P, REVELL D K, et al.In vitro fermentative traits of Australian woody perennial plant species that may be considered as potential sources of feed for grazing ruminants[J].Animal Feed Science and Technology, 2010, 160(3/4):98-109.
[10] 赵能武, 丁永芳, 杨玉涛.新疆、云南、贵州民族医药研究及中草药资源概况[J].贵阳中医学院学报, 2019, 41(2):101-103. ZHAO N W, DING Y F, YANG Y T.Overview of ethnic medicine research and Chinese herbal medicine resources in Xinjiang, Yunnan and Guizhou[J].Journal of Guiyang College of Traditional Chinese Medicine, 2019, 41(2):101-103.(in Chinese)
[11] 吴素芬, 等.中草药抑菌活性成分研究进展[J].中国保健健康, 2014, 24(2):1185-1186. WU S F.Research progress of antibacterial active constituents of Chinese herbal medicine[J].China Health Care & Nutrition, 2014, 24(2):1185-1186.(in Chinese)
[12] 刘美.瘤胃甲烷减排中草药筛选及目标中草药的作用机制研究[D].博士学位论文.杭州:浙江大学, 2016. LIU M.Screening of Chinese herbal medicine and mechanisms of target herb on methane mitigation in rumen[D].Ph.D.Thesis.Hangzhou:Zhejiang University, 2016.(in Chinese)
[13] 张倩.野葛根饲喂奶牛的适宜性评价[D].硕士学位论文.郑州:郑州大学, 2011. ZHANG Q.The assessment of suitability in cow feeding with the wild kudzu root[D].Master's Thesis.Zhengzhou:Zhengzhou University, 2011.(in Chinese)
[14] 潘龙.柴胡提取物缓解奶牛热应激的应用效果研究[D].硕士学位论文.北京:中国农业科学院, 2014. PAN L.Research on the application of Radix Bupleuri extract supplementation relieving heat-stressed lactating cows[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2014.(in Chinese)
[15] 黄涛.金银花提取物对夏季高温条件下肉牛骨骼肌纤维形态和肌肉品质的影响[D].硕士学位论文.南昌:江西农业大学, 2015. HUANG T.Effect of honeysuckle extract on skeletal muscle morphology and meat quality in beef cattle under hot environment[D].Master's Thesis.Nanchang:Jiangxi Agricultural University, 2015.(in Chinese)
[16] 符运斌.金银花绿原酸的提取及其对热应激肉牛抗氧化功能的影响研究[D].硕士学位论文.南昌:江西农业大学, 2016. FU Y B.Extraction of chlorogenic acid from honeysuckle and influence on antioxidant functions in beef cattle under heat stress[D].Master's Thesis.Nanchang:Jiangxi Agricultural University, 2016.(in Chinese)
[17] 窦德明, 代维正, 陈振峰, 等.秦岭不同产地葛根中葛根异黄酮含量的研究[J].中草药, 2002(7):90-91. DOU D M, DAI W Z, CHEN Z F, et al.The study on puerarin isoflavone content of Lobed Kudzuvine root from different areas of Qinling Mountains[J].Chinese Traditional and Herbal Drugs, 2002(7):90-91.(in Chinese)
[18] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社, 2015. Chinese Pharmacopoeia Cmommission.Pharmacopoeia of the People's Republic of China (part Ⅰ)[M].Beijing:China Medical Science Press, 2015.(in Chinese)
[19] 郭泰麟, 康廷国, 张慧.不同产地南北柴胡中柴胡皂苷的含量测定[J].天津中医药大学学报, 2020, 39(2):221-225. GUO T L, KANG T G, ZHANG H.Content determination of saikosaponins in Bupleuri Radix from different habitats[J].Journal of Tianjin University of Traditional Chinese Medicine, 2020, 39(2):221-225.(in Chinese)
[20] 许兰杰, 梁慧珍, 余永亮, 等.我国金银花绿原酸含量及影响因子研究进展[J].安徽农业科学, 2017, 45(30):125-127, 175. XU L J, LIANG H Z, YU Y L, et al.Research progress on content and influencing factors of chlorogenic acid in Lonicera japonica in China[J].Journal of Anhui Agricultural Sciences, 2017, 45(30):125-127, 175.(in Chinese)
[21] 张荣, 刘睿, 刘启德, 等.钩藤中钩藤碱、异钩藤碱的提取与含量测定[J].中药新药与临床药理, 2009, 20(4):338-341. ZHANG R, LIU R, LIU Q D, et al.Extraction and determination of rhynchophylline and isorhynchophylline in Ramulus Uncariae cum Uncis[J].Traditional Chinese Drug Research and Clinical Pharmacology, 2009, 20(04):338-341.(in Chinese)
[22] MENKE K H, RAAB L, SALEWSKI A, et al.The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro[J].The Journal of Agricultural Science, 1979, 93(1):217-222.  
[23] 张丽英.饲料分析及饲料质量检测技术[M].4版.北京:中国农业大学出版社, 2016. ZHANG L Y.Feed analysis and quality test technology[M].4th ed.Beijing:China Agricultural University Press, 2016.(in Chinese)
[24] 屯妮萨·麦提赛伊迪, 阿里娅古丽·依布热依木, 阿依沙依拉, 等.碱性次氯酸钠-苯酚分光光度法测定甲醇处理的瘤胃液中氨态氮浓度[J].新疆农业科学, 2012, 49(3):565-570. MAITISAIYIDI T, YIBUREYIMU A, AYISHAYILA, et al.Determination of ammonia-nitrogen in ruminal fluid treated with methanol by alkaline hypochlorite-phenol spectrophotometry[J].Xinjiang Agricultural Sciences, 2012, 49(3):565-570.(in Chinese)
[25] 庄二林.苜蓿与谷草组合效应的评定研究[D].硕士学位论文.杨凌:西北农林科技大学, 2017. ZHUANG E L.Evaluate of associate effects between alfalfa hay and millet stalk[D].Master's Thesis.Yangling:Northwest A & F University, 2017.(in Chinese)
[26] 苏汉书, 熊本海, 方洛云, 等.青蒿提取物对奶牛瘤胃体外发酵及甲烷生成的影响[J].北京农学院学报, 2020, 35(1):60-66. SU H S, XIONG B H, FANG L Y, et al.Effects of artemisinin on rumen fermentation and methane production in dairy cows[J].Journal of Beijing University of Agriculture, 2020, 35(1):60-66.(in Chinese)
[27] SATTER L D, SLYTER L L.Effect of ammonia concentration of rumen microbial protein production in vitro[J].British Journal of Nutrition, 1974, 32(2):199-208.  
[28] 邹新平, 曹志军, 许庆方, 等.不同粗饲料组合和精粗比对断奶犊牛瘤胃液体外发酵及微生物蛋白的影响[J].饲料研究, 2015(6):33-37. ZOU X P, CAO Z J, XU Q F, et al.Effects of different roughage combinations and concentrate-roughage ratio on rumen fermentation in vitro and microbial protein of weaned calves[J].Feed Research, 2015(6):33-37.(in Chinese)
[29] DIJKSTRA J, TAMMINGA S.Simulation of the effects of diet on the contribution of rumen protozoa to degradation of fibre in the rumen[J].British Journal of Nutrition, 1995, 74(5):617-634.  
[30] MATHIEU F, JOUANY J P, SÉNAUD J, et al.The effect of Saccharomyces cerevisiae and Aspergillus oryzae on fermentations in the rumen of faunated and defaunated sheep:protozoal and probiotic interactions[J].Reproduction, Nutrition, Development, 1996, 36(3):271-287.  
[31] 郭林新, 马养民, 强涛涛, 等.植物单宁的结构改性研究进展[J].化工学报, 2021, 72(05):2448-2464. GUO L X, MA Y M, QIANG T T, et al.Review on structure modification of plant tannins[J]. CIESC Journal, 2021, 72(05):2448-2464.(in Chinese)
[32] 武晓丹, 金哲雄, 孙素琴, 等.七种不同产地仙鹤草原药材及提取物的红外光谱与二维相关红外光谱的分析与鉴定[J].光谱学与光谱分析, 2010, 30(12):3222-3227. WU X D, JIN Z X, SUN S Q, et al.Discrimination of seven species of Agrimonia pilosa Ledeb and its extracts by FTIR and 2D-IR[J]. Spectroscopy and Spectral Analysis, 2010, 30(12):3222-3227.(in Chinese)
[33] 杨凯.单宁酸对肉牛瘤胃发酵、微生物区系、甲烷排放及氮排泄的调控规律[D].博士学位论文.北京:中国农业大学, 2017. YANG K.Manipulation of tannic acid on rumen fermentation, microbial flora, methane emission and nitrogen excretion in beef cattle[D].Ph.D.Thesis.Beijing:China Agricultural University, 2017.(in Chinese)
[34] PIÑEIRO-VÁZQUEZ A T, CANUL-SOLÍS J R, ALAYÓN-GAMBOA J A, et al.Potential of condensed tannins for the reduction of emissions of enteric methane and their effect on ruminant productivity[J].Archivos de Medicina Veterinaria, 2015, 47(3):263-272.  
[35] 唐志文, 蒋林树, 杨亮, 等.金银花提取物对瘤胃体外发酵参数及产气量的影响[J].动物营养学报, 2018, 30(2):790-796. TANG Z W, JIANG L S, YANG L, et al.Effects of honeysuckle extract on rumen in vitro fermentation parameters and gas production[J].Chinese Journal of Animal Nutrition, 2018, 30(2):790-796.(in Chinese)
[36] 殷云浩.不同氮硫比尿素饲粮条件下小肽对南江黄羊瘤胃体外发酵特性的影响[D].硕士学位论文.雅安:四川农业大学, 2012. YIN Y H.Effects of small peptide on in vitro rumen fermentation characteristics of Nanjiang brown goat under the condition of different nitrogen-sulfur ratio of urea diet[D].Master's Thesis.Ya'an:Sichuan Agricultural University, 2012.(in Chinese)
[37] 冯仰廉.反刍动物营养学[M].北京:科学出版社, 2004. FENG Y L.Ruminant nutrition[M].Beijing:Science Press, 2004.(in Chinese)
[38] 黄祥元, 唐伟, 龙冰雁.金银花提取物对肉牛瘤胃体外发酵、挥发性脂肪酸组成及气体产生的影响[J].中国饲料, 2019(24):55-58. HUANG X Y, TANG W, LONG B Y.Effects of honeysuckle extract on rumen fermentation, volatile fatty acid composition and gas production in beef cattle[J].China Feed, 2019(24):55-58.(in Chinese)
[39] 潘龙, 牛俊丽, 卜登攀, 等.柴胡皂苷对体外发酵参数及细菌数量变化的影响[J].草业学报, 2015, 24(6):85-91. PAN L, NIU J L, BU D P, et al.Effects of saikosaponin on in vitro fermentation parameters and bacterial quantity[J].Acta Prataculturae Sinica, 2015, 24(6):85-91.(in Chinese)
[40] ROOKE J A, WALLACE R J, DUTHIE C A, et al.Hydrogen and methane emissions from beef cattle and their rumen microbial community vary with diet, time after feeding and genotype[J].British Journal of Nutrition, 2014, 112(3):398-407.  
[41] 李琳, 董国忠.反刍动物瘤胃甲烷产生及调控的研究进展[J].草食家畜, 2010(2):41-44. LI L, DONG G Z.The study of methane biochemistry mechanism and relation for ruminants[J].Grass-Feeding Livestock, 2010(2):41-44.(in Chinese)
[42] 杨金山, 岳奎忠, 刘鑫, 等.利用体外模拟瘤胃发酵技术预测奶牛生产性能的研究[J].中国畜牧兽医, 2018, 45(8):2167-2174. YANG J S, YUE K Z, LIU X, et al.Prediction of dairy cow production performance by in vitro fermentation technology[J].China Animal Husbandry & Veterinary Medicine, 2018, 45(8):2167-2174.(in Chinese)
[43] NEWBOLD C J, RODE L M.Dietary additives to control methanogenesis in the rumen[J].International Congress Series, 2006, 1293:138-147.
[44] KIM E T, KIM C H, MIN K S, et al.Effects of plant extracts on microbial population, methane emission and ruminal fermentation characteristics in in vitro[J].Asian-Australasian Journal of Animal Sciences, 2012, 25(6):806-811.  
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