动物营养学报 >

2022 , Vol. 34 >Issue 6: 3743 - 3757

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.06.035

研究论文 RESEARCHPAPER

饲粮中添加布拉迪酵母对安格斯犊牛生长性能、血清免疫指标及粪便微生物区系的影响

  • 薛帅帅 ,
  • 李亚飞 ,
  • 李可瑶 ,
  • 刘浩宇 ,
  • 黄彩云 ,
  • 郭娉婷 ,
  • 方绍明 ,
  • 梁学武 ,
  • 池春梅 ,
  • 甘乾福
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  • 1. 福建农林大学动物科学学院(蜂学学院), 福州 350002;
    2. 宁德市农业科学研究所, 宁德 355017
薛帅帅(1996—),男,陕西延安人,硕士研究生,动物营养与饲料科学专业。E-mail:1057494785@qq.com

收稿日期: 2021-11-18

  网络出版日期: 2022-06-14

基金资助

福建省科技厅星火项目（2019S0036）；福建省科技厅引导性项目（2019N0069）；福建农林大学乡村振兴服务团队-草食动物产业服务团队（11899170150）；福建农林大学科技创新专项基金项目（CXZX2020057A）；福建农林大学现代农业装备福建省高校工程研究中心开放基金（MAE-201903）

收起

Effects of Dietary Supplementation of Saccharomyces boulardii on Growth Performance, Serum Immune Indexes and Fecal Microflora of Angus Calves

  • XUE Shuaishuai ,
  • LI Yafei ,
  • LI Keyao ,
  • LIU Haoyu ,
  • HUANG Caiyun ,
  • GUO Pingting ,
  • FANG Shaoming ,
  • LIANG Xuewu ,
  • CHI Chunmei ,
  • GAN Qianfu
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  • 1. College of Animal Science (College of Bee Science), Fujian Agricultural and Forestry University, Fuzhou 350002, China;
    2. Ningde Institute of Agricultural Science, Ningde 355017, China

Received date: 2021-11-18

  Online published: 2022-06-14

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摘要

本试验旨在研究饲粮中添加布拉迪酵母对安格斯犊牛生长性能、血清免疫指标及粪便微生物区系的影响。试验选取（152±10）日龄、体重相近的健康安格斯犊牛24头，随机分为3组，每组8头牛。对照组（CG组）饲喂基础饲粮，试验组分别在基础饲粮中添加100（LG组）和200 mg/kg布拉迪酵母（HG组）。预试期14 d，正试期28 d。结果表明：1） LG和HG组犊牛终末体重和平均日增重较CG组差异不显著（P>0.05）。2） LG组犊牛体高较CG组显著提高（P<0.05）。3） LG和HG组犊牛血清白细胞介素-6（IL-6）含量较CG组极显著下降（P<0.01），且HG组显著高于LG组（P<0.05）；LG组犊牛血清肿瘤坏死因子-α（TNF-α）含量较CG组显著下降（P<0.05），且HG组极显著高于LG组（P<0.01）。4） LG组Shannon指数较CG组显著上升（P<0.05）。5）在门水平上，3组的优势菌门均为厚壁菌门和拟杆菌门。在科水平上，3组的优势菌科均为瘤胃球菌科、理研菌科、毛螺菌科、普雷沃氏菌科。LG组梭菌目_vadinBB60群的相对丰度显著高于CG组（P<0.05），而丹尼丝菌科的相对丰度显著低于CG组（P<0.05）。HG组丹尼丝菌科的相对丰度显著高于CG组（P<0.05），而梭菌目_vadinBB60群的相对丰度显著低于CG组（P<0.05）。在属水平上，3组的优势菌属均为瘤胃球菌科_UCG-005、理研菌科_RC9_肠道群、瘤胃球菌科_UCG-010、拟杆菌属、艾克曼菌属。LG和HG组dgA-11_gut_group的相对丰度显著低于CG组（P<0.05）。线性判别分析效应量（LEfSe）分析结果显示，LG和HG组芽孢杆菌科、芽孢杆菌属等的相对丰度增加。综上所述，饲粮中添加布拉迪酵母对安格斯犊牛的生长性能无显著影响，可提高犊牛体高，降低血清促炎细胞因子IL-6和TNF-α含量，增强机体免疫功能，提高芽孢杆菌属等有益菌的相对丰度，改善粪便微生物区系，促进犊牛健康。本试验中，饲粮中添加100 mg/kg布拉迪酵母的效果最佳。

关键词： 犊牛; 布拉迪酵母; 生长性能; 免疫; 微生物区系

本文引用格式

薛帅帅 , 李亚飞 , 李可瑶 , 刘浩宇 , 黄彩云 , 郭娉婷 , 方绍明 , 梁学武 , 池春梅 , 甘乾福 . 饲粮中添加布拉迪酵母对安格斯犊牛生长性能、血清免疫指标及粪便微生物区系的影响[J]. 动物营养学报, 2022 , 34(6) : 3743 -3757 . DOI: 10.3969/j.issn.1006-267x.2022.06.035

Abstract

The aim of this experiment was to study the effects of dietary supplementation of Saccharomyces boulardii on growth performance, serum immune indexes and fecal microflora of Angus calves. Twenty-four Angus calves aged about (152±10) days of age with similar body weight were selected and randomly divided into 3 groups with 8 calves in each group. Calves in the control group (CG group) were fed a basal diet, and the others in experimental groups were fed the basal diet supplemented with 100 (LG group) and 200 mg/kg Saccharomyces boulardii (HG group), respectively. The pre-feeding period was 14 days and the trial period was 28 days. The results showed as follows:1) compared with the CG group, the final body weight and average daily gain of the LG and HG groups were not significantly different (P>0.05). 2) The body height of calves in the LG group was significantly higher than that in the CG group (P<0.05). 3) The serum interleukin-6 (IL-6) content of calves in LG and HG groups was extremely significant lower than that in the CG group (P<0.01), and the HG group was significantly higher than the LG group (P<0.05). The serum tumor necrosis factor-α (TNF-α) content of calves in the LG group was extremely significant lower than that in the CG group (P<0.01), and the HG group was highly significant higher than the LG group (P<0.01). 4) The Shannon index in the LG group was significantly higher than that in the CG group (P<0.05). 5) At the phylum level, the dominant phyla of three groups were Firmicutes and Bacteroidetes. At the family level, the dominant family of three groups were Ruminococcaceae, Rikenellaceae, Lachnospiraceae and Prevotellaceae etc. The relative abundance of Clostridiales_vadinBB60_group in the LG group was significantly higher than that in the CG group (P<0.05), while the relative abundance of Erysipelotrichaceae was significantly lower than that in the CG group (P<0.05). The relative abundance of Erysipelotrichaceae in the HG group was significantly higher than that in the CG group (P<0.05), while the relative abundance of Clostridiales_vadinBB60_group was significantly lower than that in the CG group (P<0.05). At the genus level, the dominant genus of three groups were Ruminococcaceae_UCG-005, Rikenellaceae_RC9_gut_group, Ruminococcaceae_UCG-010, Bacteroides and Akkermansia. The relative abundance of dgA-11_gut_group in LG and HG groups was significantly lower than that in the CG group (P<0.05). The results of LEfSe analysis showed that the relative abundance of Bacillaceae and Bacillus in LG and HG groups increased. It is concluded that dietary supplementation of Saccharomyces boulardii has no significant effect on growth performance of Angus calves, increases the body height of calves, reduces the serum proinflammatory cytokine IL-6 and TNF-α contents, and enhances the body's immunity function, raises the relative abundance of beneficial bacteria such as Bacillus, improves the fecal microflora, and promotes the health of calves. In this experiment, dietary supplementation of Saccharomyces boulardii of 100 mg/kg has the best effect.

Key words： calves; Saccharomyces boulardii; growth performance; immune; microflora

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