动物营养学报 >

2022 , Vol. 34 >Issue 6: 3896 - 3905

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.06.049

研究论文 RESEARCHPAPER

“葡萄糖氧化酶+过氧化氢酶+柠檬酸”组合添加剂对商品肉兔生产性能、免疫指标及养分表观消化率的影响

  • 詹海杰 ,
  • 李燕平 ,
  • 曹亮 ,
  • 党文庆 ,
  • 樊爱芳 ,
  • 任克良
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  • 山西农业大学动物科学学院, 太原 030032
詹海杰(1978—),男,山西清徐人,助理研究员,学士,研究方向为家兔营养、饲料资源开发等。E-mail:122714178@qq.com

收稿日期: 2021-11-04

  网络出版日期: 2022-06-14

基金资助

财政部和农业农村部国家现代农业产业技术体系（CARS-43-B-3）；山西农业大学学术恢复科研专项项目（2020xshf13）；山西农业大学畜牧专项科研项目（YCX2021XMZX2）

收起

Effects of “Glucose Oxidase+Catalase+Citric Acid” Combined Additives on Performance, Immune Indexes and Nutrient Apparent Digestibility of Commercial Meat Rabbits

  • ZHAN Haijie ,
  • LI Yanping ,
  • CAO Liang ,
  • DANG Wenqing ,
  • FAN Aifang ,
  • REN Keliang
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  • College of Animal Science, Shanxi Agricultural University, Taiyuan 030032, China

Received date: 2021-11-04

  Online published: 2022-06-14

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摘要

本试验旨在研究饲粮中添加不同剂量的"葡萄糖氧化酶+过氧化氢酶+柠檬酸"组合添加剂替代抗生素对商品肉兔生长性能、免疫器官指数、血清免疫指标、腹泻率、死亡率和养分表观消化率的影响。试验选取健康的35日龄断奶伊拉商品肉兔300只（公母各占1/2），按照平均体重和性别随机分为5组，每组60个重复，每个重复1只兔。试验设空白对照组、抗生素对照组（基础饲粮+恩拉霉素20 g/t+金霉素52.5 g/t）、试验1组[基础饲粮+低剂量组合添加剂（葡萄糖氧化酶200 g/t+过氧化氢酶114 g/t+柠檬酸1.0%）]、试验2组[基础饲粮+中剂量组合添加剂（葡萄糖氧化酶400 g/t+过氧化氢酶228 g/t+柠檬酸1.0%）]、试验3组[基础饲粮+高剂量组合添加剂（葡萄糖氧化酶800 g/t+过氧化氢酶456 g/t+柠檬酸1.0%）]。试验持续35 d，其中预试期5 d，正试期30 d。结果显示：1）饲粮中添加不同剂量的组合添加剂对肉兔的末重、平均日增重、平均日采食量及料重比均没有显著影响（P>0.05）；肉兔的腹泻率、死亡率各组之间差异不显著（P>0.05），但随着组合添加剂添加剂量的增加，腹泻率、死亡率有降低的趋势。2）试验2组的粗蛋白质表观消化率最低，与抗生素对照组差异显著（P<0.05），其他组间差异不显著（P>0.05）；抗生素对照组粗灰分、中性洗涤纤维、酸性洗涤纤维的表观消化率均显著（P<0.05）或极显著（P<0.01）高于空白对照组及各试验组，空白对照组与各试验组差异不显著（P>0.05）。3）试验3组的圆小囊指数最大，与抗生素对照组、试验2组差异极显著（P<0.01）；试验3组的血清免疫球蛋白A （IgA）含量与空白对照组、抗生素对照组差异显著（P<0.05）；试验2组、试验3组的血清分泌型免疫球蛋白A （sIgA）含量较高，与空白对照组差异极显著（P<0.01）；试验2组的血清白细胞介素-10（IL-10）含量最高，与空白对照组差异显著（P<0.05）。综上可知，随着饲粮中"葡萄糖氧化酶+过氧化氢酶+柠檬酸"组合添加剂添加剂量的增加，商品肉兔的死亡率呈下降趋势，免疫力呈上升趋势。综合分析生产性能、养分表观消化率和免疫指标，本试验条件下组合添加剂的适宜剂量为葡萄糖氧化酶800 g/t+过氧化氢酶456 g/t+柠檬酸1.0%。

关键词： 复合酶; 柠檬酸; 肉兔; 生长性能; 免疫; 养分表观消化率

本文引用格式

詹海杰 , 李燕平 , 曹亮 , 党文庆 , 樊爱芳 , 任克良 . “葡萄糖氧化酶+过氧化氢酶+柠檬酸”组合添加剂对商品肉兔生产性能、免疫指标及养分表观消化率的影响[J]. 动物营养学报, 2022 , 34(6) : 3896 -3905 . DOI: 10.3969/j.issn.1006-267x.2022.06.049

Abstract

The objective of this study was to investigate the effects of different dosage of "glucose oxidase+catalase+citric acid" combined additives in the diets to replace antibiotic on the growth performance, immune organ indexes, serum immune indexes, diarrhea rate, mortality and nutrient apparent digestibility of commercial meat rabbits. Three hundred 35-day-old weaned Yila commercial meat rabbits (half male and half female) were randomly divided into 5 groups according to body weight and sex. Each group consisted of 60 replicates with 1 rabbit per replicate. The control group included a blank control group (basal diet), an antibiotic control group (basal diet+enramycin 20 g/t+aureomycin 52.5 g/t). And the experimental groups contained experimental group 1[basal diet+low dosage combined additives (glucose oxidase 200 g/t+catalase 114 g/t+citric acid 1.0%)], experimental group 2[basal diet+medium dosage combined additives (glucose oxidase 400 g/t+catalase 228 g/t+citric acid 1.0%)] and experimental group 3[basal diet+high dosage combined additives (glucose oxidase 800 g/t+ catalase 456 g/t+citric acid 1.0%)]. The experiment lasted for 35 days, of which the pre-test period was 5 days and the trial period was 30 days. The results showed as follows:1) different dosage of combined additives in the diets had no significant differences in the final body weight, average daily gain and feed/gain (P>0.05). Similarly, there was no significant difference in diarrhea rate and mortality among groups (P>0.05), but the diarrhea rate and mortality showed a downward trend with the increase of combined additives dosage. 2) The crude protein apparent digestibility of the experimental group 2 was the lowest, which was significantly different from that of antibiotic control group (P<0.05), but there was no significant difference among other groups (P>0.05). The apparent digestibility of ash, neutral detergent fiber and acid detergent fiber of the antibiotic control group were significantly (P<0.05) or extremely significantly (P<0.01) higher than those of the blank control group and three experimental groups. In addition, there was no significant difference among the blank control groups and experimental groups (P>0.05). 3) The sacculus rotundus index of experimental group 3 was the largest, which was significantly different from the antibiotic control group and experimental group 2 (P<0.01). There was significant difference in serum immunoglobulin A (IgA) content between experimental group 3 and blank control group, antibiotic control group (P<0.05). The serum secretory immunoglobulin A (sIgA) content in experimental groups 2 and 3 was extremely significantly higher than that of blank control group (P<0.01). There was significant difference in serum interleukin-10 (IL-10) content between the experimental group 3 and the blank control group (P<0.05). In conclusion, the immunity and mortality of commercial meat rabbits show an upward trend and a downward trend with the increase of "glucose oxidase+catalase+citric acid" combined additives dosage in diets, respectively. Considering the performance, nutrient apparent digestibility and immune indexes, the suitable dosage of combined additives in the diet is glucose oxidase 800 g/t+catalase 456 g/t+citric acid 1.0% under this experimental condition.

Key words： complex enzyme; citric acid; meat rabbit; growth performance; immune; nutrient apparent digestibility (责任编辑 菅景颖)

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