动物营养学报 >

2022 , Vol. 34 >Issue 6: 3962 - 3971

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.06.055

研究论文 RESEARCHPAPER

酵母培养物促进干细胞增殖分化、改善小鼠肠上皮完整性

  • 张得香 ,
  • 朱超 ,
  • 周加义 ,
  • 王修启
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  • 华南农业大学动物科学学院, 广东省动物营养调控重点实验室, 国家生猪种业工程技术研究中心, 广州 510642
张得香(1994—),女,甘肃靖远人,硕士研究生,从事动物营养与饲料科学研究。E-mail:1554998621@qq.com

收稿日期: 2021-12-06

  网络出版日期: 2022-06-14

基金资助

国家自然科学基金面上项目（31872389，32072777）；广东省基础与应用基础研究基金项目（2019B1515210021）

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Yeast Culture Promotes Stem Cell Proliferation and Differentiation and Improves Intestinal Epithelial Integrity of Mice

  • ZHANG Dexiang ,
  • ZHU Chao ,
  • ZHOU Jiayi ,
  • WANG Xiuqi
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  • National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2021-12-06

  Online published: 2022-06-14

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摘要

本研究旨在探讨酵母培养物（YC）对小鼠肠道干细胞驱动隐窝绒毛轴更新的影响。试验选取6周龄体重[（19.85±1.45） g]相近的健康C57BL/6雄性小鼠36只，随机分为3组，每组12个重复，每个重复1只。对照组饲喂基础饲粮，0.5%和1.0% YC组分别在基础饲粮中添加0.5%和1.0% YC。试验期10 d。结果显示：1）与对照组相比，0.5% YC组小鼠平均日采食量（ADFI）极显著增加（P<0.01），1.0% YC组小鼠平均日增重（ADG）极显著增加（P<0.01），1.0% YC组小鼠料重比（F/G）显著降低（P<0.05）。2）与对照组相比，1.0% YC组小鼠十二指肠和空肠单位长度重量显著增加（P<0.05）。3）与对照组相比，0.5%和1.0% YC组小鼠空肠总超氧化物岐化酶（T-SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性显著或极显著增加（P<0.05或P<0.01），1.0% YC组小鼠空肠丙二醛（MDA）含量显著降低（P<0.05）。4）与对照组相比，0.5%和1.0% YC组小鼠空肠绒毛高度（VH）和绒毛高度/隐窝深度（VH/CD）显著增加（P<0.05）。5）与对照组相比，0.5%和1.0% YC组小鼠空肠带状闭合蛋白-1（ZO-1）和闭合蛋白（Occludin）荧光信号强度极显著增加（P<0.01），0.5%和1.0% YC组小鼠空肠ZO-1和Occludin相对表达量极显著上调（P<0.01）。6）与对照组相比，0.5%和1.0% YC组小鼠空肠增殖细胞核抗原（PCNA）荧光信号强度极显著增加（P<0.01），而小鼠空肠裂解半胱天冬酶-3（cleaved caspase-3）荧光信号强度极显著降低（P<0.01）。7）与对照组相比，0.5%和1.0% YC组小鼠空肠角蛋白20（KRT20）、绒毛蛋白（Villin）、嗜铬粒蛋白A （CGA）荧光信号强度和溶菌酶（LYZ）阳性细胞数量显著或极显著增加（P<0.05或P<0.01）。由此可见，饲粮中添加YC能促进肠道干细胞增殖和分化，抑制细胞凋亡，改善肠道结构和屏障功能，增强肠道抗氧化能力，提高小鼠生长性能。

关键词： 酵母培养物; 肠道干细胞; 肠上皮; 增殖分化

本文引用格式

张得香 , 朱超 , 周加义 , 王修启 . 酵母培养物促进干细胞增殖分化、改善小鼠肠上皮完整性[J]. 动物营养学报, 2022 , 34(6) : 3962 -3971 . DOI: 10.3969/j.issn.1006-267x.2022.06.055

Abstract

The aim of this study was to investigate the effects of yeast culture (YC) on crypt villus axis renewal driven by intestinal stem cells of mice. Thirty-six healthy 6-week-old male C57BL/6 mice with similar body weight[(19.85±1.45) g] were randomly divided into 3 groups with 12 replicates per group and 1 mouse per replicate. Mice in the control group were fed a basal diet, and others in 0.5% and 1.0% YC groups were fed the basal diet supplemented with 0.5% and 1.0% YC, respectively. The experiment lasted for 10 days. The results showed as follows:1) compared with the control group, the average daily feed intake (ADFI) of mice of 0.5% YC group was significantly increased (P<0.01), the average daily gain (ADG) of mice of 1.0% YC group was significantly increased (P<0.01), and the ratio of feed to gain (F/G) of mice of 1.0% YC group was significantly decreased (P<0.05). 2) Compared with the control group, the duodenum and jejunum weight per unit length of mice of 0.5% and 1.0% YC groups was significantly increased (P<0.05). 3) Compared with the control group, the activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.05 or P<0.01), and the jejunum malondialdehyde (MDA) content of mice of 1.0% YC group was significantly decreased (P<0.05). 4) Compared with the control group, the villus height (VH) and villus height/crypt depth (VH/CD) in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.05). 5) Compared with the control group, the fluorescence signal intensities of zonula occludens protein-1 (ZO-1) and Occludin in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.01), and the relative expression levels of ZO-1 and Occludin in jejunum of mice of 0.5% and 1.0% YC groups were significantly up-regulated (P<0.01). 6) Compared with the control group, the fluorescence signal intensity of proliferating nuclear antigen (PCNA) in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.01), while the fluorescence signal intensity of cleaved caspase-3 in jejunum of mice were significantly decreased (P<0.01). 7) Compared with the control group, the fluorescence signal intensities of keratin 20 (KRT20), Villin, chromogranin A (CGA) and positive cell number of lysozyme (LYZ) in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.05 or P<0.01). In conclusion, dietary YC can increase the proliferation and differentiation of intestinal stem cells, inhibite the cell apoptosis, improve the intestinal structure and barrier function, enhance the intestinal antioxidant capacity, and improve the growth performance of mice.

Key words： yeast culture; intestinal stem cells; intestinal epithelium; proliferation and differentiation

参考文献

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