动物营养学报 >

2022 , Vol. 34 >Issue 6: 3983 - 3990

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.06.057

研究论文 RESEARCHPAPER

猪去氧胆酸对小鼠生长性能、能量代谢及脂肪消化吸收的影响

  • 宋敏 ,
  • 马现永 ,
  • 张枫琳 ,
  • 王超普 ,
  • 苏含 ,
  • 江青艳 ,
  • 王松波
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  • 1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
    2. 华南农业大学动物科学学院, 广州 510642;
    3. 岭南现代农业科学与技术广东省实验室茂名分中心, 茂名 525000;
    4. 佛山科学技术学院生命科学与工程学院, 佛山 528225
宋敏(1985—),女,山东德州人,博士,从事动物营养与饲料和畜禽生态养殖的研究。E-mail:songmin@gdaas.cn

收稿日期: 2021-11-04

  网络出版日期: 2022-06-14

基金资助

国家自然科学基金项目（31972636，32172682）；广东省农业科学院学科团队建设项目（202118TD）；饲料产业技术体系创新团队建设项目（2021KJ115）

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Effects of Hyodeoxycholic Acid on Growth Performance, Energy Metabolism and Fat Digestion and Absorption of Mice

  • SONG Min ,
  • MA Xianyong ,
  • ZHANG Fenglin ,
  • WANG Chaopu ,
  • SU Han ,
  • JIANG Qingyan ,
  • WANG Songbo
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  • 1. Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
    3. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming 525000, China;
    4. College of Life Science and Engineering, Foshan University of Science and Technology, Foshan 528225, China

Received date: 2021-11-04

  Online published: 2022-06-14

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摘要

本试验旨在探讨饲粮添加猪去氧胆酸（HDCA）对小鼠生长、能量代谢及脂肪消化吸收的影响。选用20只3周龄C57BL雄性小鼠，分为对照组和HDCA组，分别饲喂基础饲粮和添加了1.5% HDCA的试验饲粮。试验期为10周。结果表明：与对照组相比，1） HDCA对小鼠的采食量和终末体重无显著影响（P>0.05），但显著降低了小鼠的体增重（P<0.05）。此外，HDCA显著减少了小鼠的皮下脂肪组织含量（P<0.05）；2） HDCA增加了25和4℃环境温度下小鼠的褐色脂肪组织（BAT）产热（P<0.05），并提高了小鼠白天和黑夜的基础代谢，表明HDCA提高了小鼠的能量代谢；3） HDCA极显著降低了小鼠粗脂肪表观消化率（P<0.01），从而极显著增加了小鼠粪便的粗脂肪含量和粪便能值（P<0.01）；4） HDCA有降低小鼠胰腺胰脂酶相关基因胆盐刺激性脂酶（BSSL）、磷脂酶A2（PLA2）、甘油三酯脂酶（PTL）和胰脂酶相关蛋白1（PLRP1）和胰脂酶相关蛋白2（PLRP2）的mRNA相对表达量的趋势（0.05≤P<0.10）。综上所述，饲粮添加1.5%的HDCA能够通过提高小鼠的能量代谢，减少小鼠对脂肪的消化吸收，从而降低小鼠的皮下脂肪组织含量和体增重。

关键词： 猪去氧胆酸; 小鼠; 生长性能; 能量代谢; 脂肪; 消化吸收

本文引用格式

宋敏 , 马现永 , 张枫琳 , 王超普 , 苏含 , 江青艳 , 王松波 . 猪去氧胆酸对小鼠生长性能、能量代谢及脂肪消化吸收的影响[J]. 动物营养学报, 2022 , 34(6) : 3983 -3990 . DOI: 10.3969/j.issn.1006-267x.2022.06.057

Abstract

This experiment was conducted to investigate the effects of dietary hyodeoxycholic acid (HDCA) on growth, energy metabolism and fat digestion and absorption of mice. Twenty 3-week-old C57BL male mice were divided into control group and HDCA group, and were fed a basal diet and the basal diet supplemented with 1.5% HDCA, respectively. The experiment lasted for 10 weeks. The results showed that compared with the control group:1) HDCA had no significant effects on feed intake and final body weight of mice (P>0.05), but significantly decreased body weight gain of mice (P<0.05). In addition, HDCA significantly reduced the subcutaneous adipose tissue content of mice (P<0.05). 2) HDCA increased thermogenesis of brown adipose tissue (BAT) at 25 and 4℃ (P<0.05), and increased the basal metabolism in both day and night, suggesting that HDCA increased energy metabolism of mice. 3) HDCA extremely significantly reduced the apparent digestibility of crude fat of mice (P<0.01), thereby significantly increasing the crude fat content and fecal energy of mice (P<0.01). 4) HDCA tended to decrease the mRNA expression levels of pancreatic lipase-related genes bile salt-stimulating lipase (BSSL), pancreatic phospholipase A2 (PLA2), pancreatic triglyceride lipase (PTL), pancreatic lipase-related protein 1 (PLRP1) and pancreatic lipase-related protein 2 (PLRP2) of mice (0.05 ≤ P<0.10). In conclusion, dietary supplementation of 1.5% HDCA can improve the energy metabolism of mice, and reduce the digestion and absorption of fat of mice, thereby reducing the subcutaneous adipose tissue content and body weight gain of mice.

Key words： hyodeoxycholic acid; mice; growth performance; energy metabolism; fat; digestion and absorption

参考文献

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