研究论文 RESEARCH PAPER

厚朴酚对断奶仔猪营养物质表观消化率、血清生化指标、粪便主要微生物数量及其代谢产物含量的影响

  • 梅华迪 ,
  • 李袁飞 ,
  • 田琦 ,
  • 李贞明 ,
  • 容庭 ,
  • 马现永 ,
  • 田志梅 ,
  • 崔艺燕 ,
  • 余苗
展开
  • 1. 广东省农业科学院动物科学研究所, 农业部华南动物营养与饲料重点实验室, 畜禽育种国家重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东省畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
    2. 岭南现代农业科学与技术广东省实验室茂名分中心, 茂名 525000
梅华迪(1997—),男,湖南常德人,硕士研究生,从事单胃动物生态健康养殖研究。E-mail:m15973699341@163.com

收稿日期: 2022-01-12

  网络出版日期: 2022-08-11

基金资助

广东省现代农业产业技术体系饲料创新团队项目(2022KJ115);广东省农业科学院农业优势产业科学团队建设项目(202118TD);科技创新战略专项资金(高水平农科院建设)-杰出人才(R2020PY-JC001)和优秀博士(R2020YJ-YB2002);省级农业科技创新推广及农业资源与生态环境保护建设项目(2021KJ266);广东省农业科学院生猪产业研究院(2021研究院04,2022研究院04);茂名实验室自主科研项目(2021ZZ003)

Effects of Magnolol on Nutrient Apparent Digestibility, Serum Biochemical Indices, Main Fecal Microorganism Numbers and Their Metabolite Contents of Weaned Piglets

  • MEI Huadi ,
  • LI Yuanfei ,
  • TIAN Qi ,
  • LI Zhenming ,
  • RONG Ting ,
  • MA Xianyong ,
  • TIAN Zhimei ,
  • CUI Yiyan ,
  • YU Miao
Expand
  • 1. Guangdong Engineering and Technology Research Center for Quality and Safety Control and Evaluation of Livestock and Poultry Meat, Guangdong Key Laboratory of Livestock and Poultry Breeding and Nutrition, State Key Laboratory of Livestock and Poultry Breeding, South China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Maoming Branch Center of Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Maoming 525000, China

Received date: 2022-01-12

  Online published: 2022-08-11

摘要

本试验旨在探究饲粮中添加不同水平厚朴酚对断奶仔猪营养物质表观消化率、血清生化指标、粪便主要微生物数量及其代谢产物含量的影响。试验选取体重[(7.91±0.00) kg]相近且健康的21日龄"杜×长×大"三元杂交断奶仔猪144头,按体重接近原则随机分为3组,每组8个重复,每个重复6头猪。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加0.02%和0.04%厚朴酚。试验期42 d,其中预试期7 d,正试期35 d。结果显示:1)与对照组相比,饲粮中添加0.04%厚朴酚显著提高了粗蛋白质表观消化率(P<0.05)。2)与对照组相比,饲粮中添加0.04%厚朴酚显著降低了血清尿素、肌酐和甘油三酯含量(P<0.05),饲粮中添加0.02%厚朴酚显著提高了血清葡萄糖含量(P<0.05)。3)与对照组相比,饲粮中添加0.04%厚朴酚显著提高了粪便中乳杆菌属(Lactobacillus)、双歧杆菌属(Bifidobacterium)和瘤胃球菌属(Ruminococcus)数量(P<0.05),显著降低了大肠杆菌(Escherichia coli)数量(P<0.05);饲粮中添加0.02%厚朴酚显著提高了粪便中Lactobacillus数量(P<0.05),显著降低了拟杆菌门(Bacteroidetes)数量(P<0.05)。4)与对照组相比,饲粮中添加0.04%厚朴酚显著降低了粪便pH (P<0.05),显著提高了粪便中乙酸和总短链脂肪酸含量(P<0.05);饲粮中添加0.02%厚朴酚显著提高了粪便中乙酸、丙酸和总短链脂肪酸含量(P<0.05)。5)与对照组相比,饲粮中添加0.04%厚朴酚显著降低了粪便中苯酚、对甲酚、粪臭素、腐胺、尸胺和总生物胺含量(P<0.05),饲粮中添加0.02%厚朴酚显著降低了粪便中对甲酚、粪臭素、尸胺和总生物胺含量(P<0.05)。综上所述,饲粮中添加0.04%厚朴酚可提高断奶仔猪粗蛋白质表观消化率,调节机体代谢,同时调控微生物菌群结构,使后肠微生物偏向碳水化合物发酵模式。这提示饲粮中添加0.04%厚朴酚有利于促进宿主生长,并对仔猪的肠道健康发挥潜在的积极作用。

本文引用格式

梅华迪 , 李袁飞 , 田琦 , 李贞明 , 容庭 , 马现永 , 田志梅 , 崔艺燕 , 余苗 . 厚朴酚对断奶仔猪营养物质表观消化率、血清生化指标、粪便主要微生物数量及其代谢产物含量的影响[J]. 动物营养学报, 2022 , 34(8) : 4919 -4931 . DOI: 10.3969/j.issn.1006-267x.2022.08.018

Abstract

This experiment was aimed to investigate the effects of dietary different supplemental levels of magnolol on nutrient apparent digestibility, serum biochemical indices, main fecal microorganism numbers and their metabolite contents of weaned piglets. A total of 144 healthy 21-day-old "Duroc×Landrace×Large White" crossbred weaned piglets with similar body weight [(7.91±0.00) kg] were randomly divided into 3 groups with 8 replicates per group and 6 piglets per replicate. Piglets in the control group were fed a basal diet, and others in experimental groups were fed basal diets supplemented 0.02% and 0.04% magnolol, respectively. The experiment lasted for 42 days, the pre-feeding period was 7 days and the formal period was 35 days. The results showed as follows: 1) compared with the control group, dietary supplemented 0.04% magnolol significantly increased the crude protein apparent digestibility (P<0.05). 2) Compared with the control group, dietary supplemented 0.04% magnolol significantly decreased the contents of urea, creatinine and triglyceride in serum (P<0.05), and dietary supplemented 0.02% magnolol significantly increased the serum glucose content (P<0.05). 3) Compared with the control group, dietary supplemented 0.04% magnolol significantly increased the numbers of Lactobacillus, Bifidobacterium and Ruminococcus in feces (P<0.05), and significantly decreased the Escherichia coli number (P<0.05); dietary supplemented 0.02% magnolol significantly increased the fecal Lactobacillus number (P<0.05), and significantly decreased the Bacteroidetes number (P<0.05). 4) Compared with the control group, dietary supplemented 0.04% magnolol significantly decreased the fecal pH (P<0.05), and significantly increased the contents of acetate and total short-chain fatty acid in feces (P<0.05); dietary supplemented 0.02% magnolol significantly increased the contents of acetate, propionate and total short-chain fatty acids in feces (P<0.05). 5) Compared with the control group, dietary supplemented 0.04% magnolol significantly decreased the contents of phenol, p-cresol, skatole, putrescine, cadaverine and total biogenic amine in feces (P<0.05), and dietary supplemented 0.02% magnolol significantly decreased the contents of p-cresol, skatole, cadaverine and total biogenic amine in feces (P<0.05). In conclusion, dietary supplemented 0.04% magnolol can increase the crude protein apparent digestibility of weaned piglets, regulate body metabolism, and at the same time improve the microorganism structure, resulting in the trend of fermentation by hindgut microbes towards carbohydrate mode. These findings indicate that dietary supplemented 0.04% magnolol may offer potential benefits for growth performance and the gut health of the host.

参考文献

[1] 崔艺燕,田志梅,李贞明,等.植物提取物的生物学功能及其在仔猪生产上的应用[J].中国畜牧兽医,2018,45(12):3419-3430.CUI Y Y,TIAN Z M,LI Z M,et al.Progress of biological functions of plant extracts and their application in piglets[J].China Animal Husbandry&Veterinary Medicine,2018,45(12):3419-3430.(in Chinese)
[2] CHEN F,ZHANG H,DU E C,et al.Supplemental magnolol or honokiol attenuates adverse effects in broilers infected with Salmonella pullorum by modulating mucosal gene expression and the gut microbiota[J].Journal of Animal Science and Biotechnology,2021,12(1):87.
[3] LIN Q,ZHAO J F,XIE K,et al.Magnolol additive as a replacer of antibiotic enhances the growth performance of Linwu ducks[J].Animal Nutrition,2017,3(2):132-138.
[4] DU E C,FAN Q W,ZHAO N,et al.Supplemental magnolol improves the antioxidant capacity and intestinal health of broiler chickens[J].Animal Science Journal,2021,92(1):e13665.
[5] LIN Q,PENG S M,LI Y H,et al.Magnolol additive improves carcass and meat quality of Linwu ducks by modulating antioxidative status[J].Animal Science Journal,2020,91(1):e13301.
[6] 屈圣富,田琦,梅华迪,等.厚朴酚对断奶仔猪生长性能、肝脏抗氧化功能及脂代谢的影响[J].动物营养学报,2022,34(5):2884-2893.QU S F,TIAN Q,MEI H D,et al.Effects of magnolol on growth performance,liver antioxidant function and lipid metabolism of weaned piglets[J].Chinese Journal of Animal Nutrition,2022,34(5):2884-2893.(in Chinese)
[7] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.饲料中盐酸不溶灰分的测定:GB/T 23742-2009[S].北京:中国标准出版社,2009:12.General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of the People's Republic of China.Animal feeding stuffs-determination of ash insoluble in hydrochloric acid:GB/T 23742-2009[S].Beijing:Standards Press of China,2009:12.(in Chinese)
[8] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.饲料中水分和其他挥发性物质含量的测定:GB/T 6435-2006[S].北京:中国标准出版社,2007:12.General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of the People's Republic of China.Determination of moisture and other volatile mater content in feeds:GB/T 6435-2006[S].Beijing:Standards Press of China,2007:12.(in Chinese)
[9] 国家市场监督管理总局,中国国家标准化管理委员会.饲料中钙的测定:GB/T 6436-2018[S].北京:中国标准出版社,2018:12.State Administration for Market Regulation,Standardization Administration of the People's Republic of China.Determination of calcium in feeds:GB/T 6436-2018[S].Beijing:Standards Press of China,2018:12.(in Chinese)
[10] 国家市场监督管理总局,国家标准化管理委员会.饲料中总磷的测定分光光度法:GB/T 6437-2018[S].北京:中国标准出版社,2018:8.State Administration for Market Regulation,Standardization Administration of the People's Republic of China.Determination of phosphorus in feeds-spectrophotometry:GB/T 6437-2018[S].Beijing:Standards Press of China,2018:8.(in Chinese)
[11] YU M,LI Z M,CHEN W D,et al.Hermetia illucens larvae as a potential dietary protein source altered the microbiota and modulated mucosal immune status in the colon of finishing pigs[J].Journal of Animal Science and Biotechnology,2019,10(1):50.
[12] 余苗,李贞明,陈卫东,等.不同淀粉类型饲粮对育肥猪盲肠食糜主要微生物及其代谢产物的影响[J].动物营养学报,2020,32(2):613-625.YU M,LI Z M,CHEN W D,et al.Effects of different starch type diets on main microbes and their metabolites in cecal digesta of finishing pigs[J].Chinese Journal of Animal Nutrition,2020,32(2):613-625.(in Chinese)
[13] YU M,ZHANG C J,YANG Y X,et al.Long-term effects of early antibiotic intervention on blood parameters,apparent nutrient digestibility,and fecal microbial fermentation profile in pigs with different dietary protein levels[J].Journal of Animal Science and Biotechnology,2017,8:60.
[14] CHANEY A L,MARBACH E P.Modified reagents for determination of urea and ammonia[J].Clinical Chemistry,1962,8:130-132.
[15] 余苗,李贞明,王刚,等.黑水虻幼虫粉对育肥猪盲肠食糜主要微生物数量和代谢产物的影响[J].畜牧兽医学报,2020,51(2):299-310.YU M,LI Z M,WANG G,et al.Effects of Hermetia illucens larvae meal on the number of main microbes and metabolites in the cecal digesta of finishing pigs[J].Acta Veterinaria et Zootechnica Sinica,2020,51(2):299-310.(in Chinese)
[16] 王振奋,黄平,蔡国豪.厚朴酚对功能性消化不良小鼠消化液分泌和胃肠运动功能的影响[J].现代消化及介入诊疗,2020,25(4):454-458.WANG Z F,HUANG P,CAI G H.Effects of magnolol on digestive fluid secretion and gastrointestinal motion function in mice with functional dyspepsia[J].Modern Digestion&Intervention,2020,25(4):454-458.(in Chinese)
[17] CAMPBELL J M,CRENSHAW J D,POLO J.The biological stress of early weaned piglets[J].Journal of Animal Science and Biotechnology,2013,4(1):19.
[18] NICHOLSON J K,HOLMES E,KINROSS J M,et al.Metabolic phenotyping in clinical and surgical environments[J].Nature,2012,491(7424):384-392.
[19] LIU H,JI H F,ZHANG D Y,et al.Effects of Lactobacillus brevis preparation on growth performance,fecal microflora and serum profile in weaned pigs[J].Livestock Science,2015,178:251-254.
[20] 余苗,李贞明,陈卫东,等.黑水虻幼虫粉对育肥猪营养物质消化率、血清生化指标和氨基酸组成的影响[J].动物营养学报,2019,31(7):3330-3337.YU M,LI Z M,CHEN W D,et al.Effects of Hermetia illucens L.larvae meal on nutrient digestibility,serum biochemical indices and amino acid composition of finishing pigs[J].Chinese Journal of Animal Nutrition,2019,31(7):3330-3337.(in Chinese)
[21] 王杰,徐友信,屈长波,等.哺乳期补饲代乳品对仔猪生长性能、营养物质消化率及血清生化指标的影响[J].中国畜牧兽医,2016,43(12):3177-3183.WANG J,XU Y X,QU C B,et al.Effect of supplementary feeding milk replacer on growth performance,nutrient digestibility,serum biochemistry indexes in piglets during lactation[J].China Animal Husbandry&Veterinary Medicine,2016,43(12):3177-3183.(in Chinese)
[22] THONGSONG B,WIYAPORN M,KALANDAKANOND-THONGSONG S.Blood glucose,amino acid profiles and nutrient transporter gene expressions in the small intestine of low and normal birthweight piglets during the early suckling period[J].Veterinary Journal,2019,247:1-7.
[23] 巫丽娟,陈代文,毛湘冰,等.饮水中添加功能性复合添加剂对断奶仔猪生长性能、养分消化利用和血液指标的影响[J].畜牧兽医学报,2017,48(7):1365-1372.WU L J,CHEN D W,MAO X B,et al.Effects of supplementing the functional compound additive in drinking water on growth performance,nutrient digestibility and blood parameters in weaned piglets[J].Acta Veterinaria et Zootechnica Sinica,2017,48(7):1365-1372.(in Chinese)
[24] OHLAND C L,MACNAUGHTON W K.Probiotic bacteria and intestinal epithelial barrier function[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2010,298(6):G807-G819.
[25] ASHIDA H,OGAWA M,KIM M,et al.Bacteria and host interactions in the gut epithelial barrier[J].Nature Chemical Biology,2011,8(1):36-45.
[26] LEE S H,INGALE S L,KIM J S,et al.Effects of dietary supplementation with Bacillus subtilis LS 1-2 fermentation biomass on growth performance,nutrient digestibility,cecal microbiota and intestinal morphology of weanling pig[J].Animal Feed Science and Technology,2014,188:102-110.
[27] WANG W,CHEN L P,ZHOU R,et al.Increased proportions of Bifidobacterium and the Lactobacillus group and loss of butyrate-producing bacteria in inflammatory bowel disease[J].Journal of Clinical Microbiology,2014,52(2):398-406.
[28] YU M,LI Z M,CHEN W D,et al.Hermetia illucens larvae as a fishmeal replacement alters intestinal specific bacterial populations and immune homeostasis in weanling piglets[J].Journal of Animal Science,2020,98(3):skz395.
[29] CHEN X Y,SONG P X,FAN P X,et al.Moderate dietary protein restriction optimized gut microbiota and mucosal barrier in growing pig model[J].Frontiers in Cellular and Infection Microbiology,2018,8:246.
[30] MU C L,YANG Y X,SU Y,et al.Differences in microbiota membership along the gastrointestinal tract of piglets and their differential alterations following an early-life antibiotic intervention[J].Frontiers in Microbiology,2017,8:797.
[31] BARNICH N,CARVALHO F A,GLASSER A L,et al.CEACAM6 acts as a receptor for adherent-invasive E.coli,supporting ileal mucosa colonization in Crohn disease[J].The Journal of Clinical Investigation,2007,117(6):1566-1574.
[32] DAGLIA M.Polyphenols as antimicrobial agents[J].Current Opinion in Biotechnology,2012,23(2):174-181.
[33] PARKAR S G,TROWER T M,STEVENSON D E.Fecal microbial metabolism of polyphenols and its effects on human gut microbiota[J].Anaerobe,2013,23:12-19.
[34] COTTER P D,HILL C.Surviving the acid test:responses of gram-positive bacteria to low pH[J].Microbiology and Molecular Biology Reviews,2003,67(3):429-453.
[35] MA J Y,PIAO X S,SHANG Q H,et al.Mixed organic acids as an alternative to antibiotics improve serum biochemical parameters and intestinal health of weaned piglets[J].Animal Nutrition,2021,7(3):737-749.
[36] TREMAROLI V,BÄCKHED F.Functional interactions between the gut microbiota and host metabolism[J].Nature,2012,489(7415):242-249.
[37] LI R R,ZHENG M L,ZHENG M H,et al.Metagenomic analysis reveals the linkages between bacteria and the functional enzymes responsible for potential ammonia and biogenic amine production in alfalfa silage[J].Journal of Applied Microbiology,2022,132(4):2594-2604.
[38] RIST V T S,WEISS E,EKLUND M,et al.Impact of dietary protein on microbiota composition and activity in the gastrointestinal tract of piglets in relation to gut health:a review[J].Animal,2013,7(7):1067-1078.
[39] NYANGALE E P,MOTTRAM D S,GIBSON G R.Gut microbial activity,implications for health and disease:the potential role of metabolite analysis[J].Journal of Proteome Research,2012,11(12):5573-5585.
[40] DAVILA A M,BLACHIER F,GOTTELAND M,et al.Re-print of "intestinal luminal nitrogen metabolism:role of the gut microbiota and consequences for the host"[J].Pharmacological Research,2013,69(1):114-126.
[41] PI Y,GAO K,PENG Y,et al.Antibiotic-induced alterations of the gut microbiota and microbial fermentation in protein parallel the changes in host nitrogen metabolism of growing pigs[J].Animal,2019,13(2):262-272.
[42] 钟智康,王燕,吴银宝,等.单胃动物肠道细菌群落及其产臭机制研究进展[J].家畜生态学报,2018,39(6):5-11.ZHONG Z K,WANG Y,WU Y B,et al.Effect of intestinal bacteria community and its mechanism on odor production in monogastric animals[J].Acta Ecologae Animalis Domastici,2018,39(6):5-11.(in Chinese)
文章导航

/