本试验旨在研究饲粮不同含硫氨基酸(SAA)水平对育成期海兰灰蛋鸡生长发育及后续生长和产蛋性能的影响。采用单因素试验设计,选取240只体重和胫骨长度相近的9周龄海兰灰商品代蛋鸡,随机分为4个组,每组6个重复,每个重复10只鸡。对照组饲喂SAA水平为0.54%的基础饲粮,试验组分别饲喂在基础饲粮中添加0.08%、0.16%和0.24%可消化蛋氨酸(等量替换各组沸石粉)的饲粮。各组饲粮SAA水平(实测值)分别为0.54%、0.62%、0.70%和0.78%。育成期试验期为7周(9~15周龄);后续的预产蛋期和产蛋高峰期各组采用相同饲粮,持续观察到33周龄末。结果表明:1)育成期饲粮不同SAA水平对海兰灰蛋鸡的终末体重、平均日增重和料重比均无显著影响(P>0.05)。与对照组相比,0.62% SAA组蛋鸡平均日采食量显著提高(P<0.05)。2)10和12周龄,与对照组相比,试验组蛋鸡体重显著提高(P<0.05),且随着饲粮SAA水平的提高,蛋鸡体重呈线性提高(P<0.05);10周龄,与对照组相比,试验组蛋鸡胫骨长度显著增加(P<0.05),且随着饲粮SAA水平的提高,蛋鸡胫骨长度呈线性增加(P<0.05)。3)20周龄,与对照组相比,0.62% SAA组后续产蛋期蛋鸡胫骨长度显著增加(P<0.05);同时,随着育成期饲粮SAA水平的提高,后续产蛋期蛋鸡胫骨长度呈二次曲线变化(P<0.05)。22和29周龄,育成期饲粮添加不同水平的SAA对后续产蛋期海兰灰蛋鸡体重和胫骨长度均无显著影响(P>0.05)。4)育成期饲粮不同SAA水平对21~33周龄海兰灰蛋鸡产蛋性能无显著影响(P>0.05)。综合育成期蛋鸡生长发育及后续产蛋性能,海兰灰商品代蛋鸡育成期饲粮SAA水平以0.62%~0.70%为宜。
This experiment was conducted to investigate the effects of different dietary sulfur-containing amino acid (SAA) levels on growth and development and subsequent growth and laying performance of Hy-Line grey laying hens during growing period. A total of 240 Hy-Line grey commercial laying hens of 9-week-old with similar body weight and tibia length were randomly divided into 4 groups with 6 replicates per group and 10 hens per replicate. Hens in the control group were fed a basal diet with SAA level of 0.54%, and those in experimental groups were fed the basal diet supplemented with 0.08%, 0.16% and 0.24% digestible methionine (replaced zeolite powder in each group in equal quantity), respectively. Dietary SAA levels (measured values) in each group were 0.54%, 0.62%, 0.70% and 0.78%, respectively. The growing period was 7 weeks (9 to 15 weeks of age). Then the same diet was used in the subsequent pre-laying period and peak laying period, and the observation lasted until the end of 33 weeks of age. The results showed as follows: 1) different dietary SAA levels had no significant effects on the final body weight, average daily gain and feed to gain ratio of Hy-Line grey laying hens during growing period (P>0.05). Compared with the control group, the average daily feed intake of laying hens in 0.62% SAA group was significantly increased (P<0.05). 2) At 10 and 12 weeks of age, the body weight of laying hens in experimental groups was significantly increased compared with the control group (P<0.05), which was linearly increased with the increase of dietary SAA level (P<0.05). At 10 weeks of age, the tibia length of laying hens in experimental groups was significantly increased compared with the control group (P<0.05), which was linearly increased with the increase of dietary SAA level (P<0.05). 3) At 20 weeks of age, the tibia length of laying hens in 0.62% SAA group was significantly increased compared with the control group (P<0.05); meanwhile, with the increase of dietary SAA level in growing period, the tibia length in laying period showed a quadratic curve change (P<0.05). At 22 and 29 weeks of age, different dietary SAA levels during growing period had no significant effects on body weight and tibia length of Hy-Line grey laying hens during subsequent laying period (P>0.05). 4) Different dietary SAA levels during growing period had no significant effects on laying performance of Hy-Line grey laying hens from 21 to 33 weeks of age (P>0.05). Considering the growth and development during growing period and subsequent laying performance of laying hens, the optimal dietary SAA level of Hy-Line grey commercial laying hens during growing period is 0.62% to 0.70%.
[1] TESSERAUD S,MÉTAYER COUSTARD S,COLLIN A,et al.Role of sulfur amino acids in controlling nutrient metabolism and cell functions:implications for nutrition[J].British Journal of Nutrition,2009,101(8):1132-1139.
[2] BIN P,HUANG R L,ZHOU X H.Oxidation resistance of the sulfur amino acids:methionine and cysteine[J].BioMed Research International,2017,2017:9584932.
[3] 宗恩艳,熊霞,杨焕胜,等.含硫氨基酸对猪生长性能及肠道功能的影响机制[J].生命科学研究,2016,20(5):466-470.ZONG E Y,XIONG X,YANG H S,et al.The effects and mechanisms of sulfur amino acids on growth performance and intestinal function in pigs[J].Life Science Research,2016,20(5):466-470.(in Chinese)
[4] 梁中军,韩雪娇,马秋刚,等.饲粮含硫氨基酸水平对京红蛋鸡产蛋高峰期生产性能的影响[J].动物营养学报,2015,27(12):3720-3725.LIANG Z J,HAN X J,MA Q G,et al.Effects of dietary levels of sulphur amino acids on performance of Jinghong laying hens during peak production period[J].Chinese Journal of Animal Nutrition,2015,27(12):3720-3725.(in Chinese)
[5] BROSNAN J T,BROSNAN M E.The sulfur-containing amino acids:an overview[J].Journal of Nutrition,2006,136(6 Suppl):1636S-1640S.
[6] 徐良梅,曹淑鑫,刘大伟,等.含硫氨基酸在动物肠道代谢及养殖生产中应用[J].东北农业大学学报,2019,50(2):90-96.XU L M,CAO S X,LIU D W,et al.Metabolism of sulfur amino acids in animal intestinal and application in aquaculture production[J].Journal of Northeast Agricultural University,2019,50(2):90-96.(in Chinese)
[7] MAROUFYAN E,KASIM A,HASHEMI S R,et al.The effect of methionine and threonine supplementations on immune responses of broiler chickens challenged with infectious bursal disease[J].American Journal of Applied Sciences,2010,7(1):44-50.
[8] RAMA RAO S V,PRAHARAJ N K,PANDA A K,et al.Interaction between genotype and dietary concentrations of methionine for immune function in commercial broilers[J].British Poultry Science,2003,44(1):104-112.
[9] 中华人民共和国农业部.鸡饲养标准:NY/T 33-2004[S].北京:中国农业出版社,2004.Ministry of Agriculture of the PRC.Feeding standard of chicken:NY/T 33-2004[S].Beijing:China Agriculture Press,2004.(in Chinese)
[10] 林泽铃,蒋守群.家禽蛋氨酸营养研究新进展[J].广东畜牧兽医科技,2021,46(4):1-6,19.LIN Z L,JIANG S Q.New progress of methionine nutrition in poultry[J].Guangdong Journal of Animal and Veterinary Science,2021,46(4):1-6,19.(in Chinese)
[11] MATSUSHITA K,TAKAHASHI K,AKIBA Y.Effects of adequate or marginal excess of dietary methionine hydroxy analogue free acid on growth performance,edible meat yields and inflammatory response in female broiler chickens[J].The Journal of Poultry Science,2007,44(3):265-272.
[12] KIM W K,FROELICH C A,PATTERSON P H,et al.The potential to reduce poultry nitrogen emissions with dietary methionine or methionine analogues supplementation[J].World's Poultry Science Journal,2006,62(2):338-353.
[13] AHMED M E,ABBAS T E.Effects of dietary levels of methionine on broiler performance and carcass characteristics[J].International Journal of Poultry Science,2011,10(2):147-151.
[14] WU B Y,CUI H M,PENG X,et al.Effect of methionine deficiency on the thymus and the subsets and proliferation of peripheral blood T-cell,and serum IL-2 contents in broilers[J].Journal of Integrative Agriculture,2012,11(6):1009-1019.
[15] MA M L,GENG S J,LIU M L,et al.Effects of different methionine levels in low protein diets on production performance,reproductive system,metabolism,and gut microbiota in laying hens[J].Frontiers in Nutrition,2021,8:739676.
[16] LIU Z,WU G,BRYANT M M,et al.Influence of added synthetic lysine in low-protein diets with the methionine plus cysteine to lysine ratio maintained at 0.75[J].Journal of Applied Poultry Research,2005,14(1):174-182.
[17] D'AGOSTINI P,GOMES P C,CALDERANO A A,et al.Requirement of methionine+cystine for pullets in the growing phase from 7 to 12 weeks old[J].Arquivo Brasileiro De Medicina Veterinária e Zootecnia,2012,64(6):1699-1706.
[18] 索菲娅,梁中军,马秋刚,等.7~12周龄京红商品代蛋鸡饲粮含硫氨基酸需要量的研究[J].动物营养学报,2018,30(2):553-559.SUO F Y,LIANG Z J,MA Q G,et al.Dietary sulphur amino acids requirement of Jinghong commercial layers from 7 to 12 weeks of age[J].Chinese Journal of Animal Nutrition,2018,30(2):553-559.(in Chinese)
[19] CASTRO F L S,KIM Y,XU H,et al.The effect of total sulfur amino acid levels on growth performance and bone metabolism in pullets under heat stress[J].Poultry Science,2020,99(11):5783-5791.
[20] 陈曦,刘茹,赵丽红,等.育雏前期含硫氨基酸水平对育雏、育成及产蛋期"京红1号"蛋鸡生产性能的影响[J].中国畜牧杂志,2018,54(8):75-79.CHEN X,LIU R,ZHAO L H,et al.Effects of different levels of total sulfur amino acids during 0-2wk on production performance of Jinghong layers in brooding,rearing and laying period[J].Chinese Journal of Animal Science,2018,54(8):75-79.(in Chinese)
[21] 潘迎丽,孙秋娟,张晓怡,等."京粉6号"商品蛋鸡产蛋期适宜蛋白、蛋氨酸水平的研究[J].中国饲料,2021(7):92-96.PAN Y L,SUN Q J,ZHANG X Y,et al.Study on the suitable level of protein and methionine of "Jing Tint 6" commodity laying hens[J].China Feed,2021(7):92-96.(in Chinese)
[22] DA SILVA E L,DA SILVA J H V,BERTECHINI A G,et al.Methionine+cystine requirements for light and semi-heavy pullets from 1 to 4 weeks of age fed crushed or ground rations[J].Revista Brasileira de Zootecnia,2009,38(3):500-507.