动物营养学报 >

2022 , Vol. 34 >Issue 8: 5048 - 5062

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.029

研究论文 RESEARCH PAPER

山香圆叶提取物对湿热条件下文昌鸡生长性能、屠宰性能、血清免疫和抗氧化功能及肠道微生态的影响

  • 宋文静 ,
  • 宋琼莉 ,
  • 陈小连 ,
  • 刘林秀 ,
  • 谭佳 ,
  • 邹志恒 ,
  • 魏立民 ,
  • 熊平文 ,
  • 张艳 ,
  • 孙瑞萍
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  • 1. 江西省农业科学院畜牧兽医研究所, 南昌 330200;
    2. 海南省农业科学院畜牧兽医研究所, 海口 571100;
    3. 海南省热带动物繁育与疫病研究重点实验室, 海口 570203
宋文静(1989—),女,河南安阳人,助理研究员,硕士,主要从事动物营养与饲料资源开发研究。E-mail:928152727@qq.com

收稿日期: 2022-01-10

  网络出版日期: 2022-08-11

基金资助

江西省重点研发计划一般项目(20212BBF63025);国家肉鸡产业技术体系海口综合试验站项目(CARS-41-Z17);海南省热带动物繁育与疫病研究重点实验室开放课题(HKL20200201)

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Effects of Turpiniae folium Extract on Growth Performance, Slaughter Performance, Serum Immune and Antioxidant Function and Intestinal Microecology in Wenchang Chicken under Hot-Humid Conditions

  • SONG Wenjing ,
  • SONG Qiongli ,
  • CHEN Xiaolian ,
  • LIU Linxiu ,
  • TAN Jia ,
  • ZOU Zhiheng ,
  • WEI Limin ,
  • XIONG Pingwen ,
  • ZHANG Yan ,
  • SUN Ruiping
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  • 1. Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;
    2. Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100;
    3. Key Laboratory of Tropical Animal Breeding and Disease Research, Haikou 570203, China

Received date: 2022-01-10

  Online published: 2022-08-11

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摘要

本试验旨在研究饲粮中添加不同水平山香圆叶提取物(TFE)对夏季自然湿热条件下文昌鸡生长性能、屠宰性能、血清免疫和抗氧化功能及肠道微生态的影响。选取同一饲养条件下生长至84日龄、体重接近的健康文昌鸡母鸡240羽,随机分为4组,每组6个重复,每个重复10羽。对照组饲喂不添加TFE的基础饲粮,试验组饲喂在基础饲粮中分别添加0.02%、0.04%、0.08% TFE的试验饲粮,试验期为35 d,整个饲养期舍内处于湿热环境(日均温度28.26~34.80℃,日均相对湿度67.00%~87.58%)。结果表明:1)随着饲粮TFE添加水平的增加,平均日增重(ADG)呈显著线性(P=0.004)和二次曲线(P=0.001)变化。与对照组相比,试验组料重比(F/G)呈下降趋势(0.05≤P<0.10)。2)与对照组相比,试验组血清总蛋白(TP)、免疫球蛋白M (IgM)、胰岛素(INS)含量呈升高趋势(0.05≤P<0.10);随着饲粮TFE添加水平的增加,血清白细胞介素-10(IL-10)含量呈显著线性(P=0.002)和二次曲线(P=0.006)变化;血清白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)含量呈显著线性(P=0.004,P=0.005)和二次曲线(P=0.009,P=0.005)变化。与对照组相比,饲粮添加0.04%和0.08% TFE显著降低了血清葡萄糖(GLU)、肿瘤坏死因子-α(TNF-α)、热应激蛋白70(HSP70)和丙二醛(MDA)含量(P<0.05),显著提高了血清免疫球蛋白A (IgA)、免疫球蛋白G (IgG)、IL-10含量以及总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05)。3)随着饲粮TFE添加水平增加,脾脏指数呈显著二次曲线变化(P=0.043)。4)0.04%和0.08% TFE组空肠绒毛高(VH)和绒毛高度/隐窝深度(VH/CD)显著高于对照组(P<0.05)。5)随着饲粮TFE添加水平的增加,盲肠变形菌门相对丰度呈显著线性(P=0.016)和二次曲线(P=0.011)变化。与对照组相比,试验组双歧杆菌属相对丰度呈增加趋势(0.05≤P<0.10)。与对照组相比,饲粮添加0.04%和0.08% TFE显著降低了盲肠梭杆菌门和普雷沃氏菌科_UCG-001相对丰度(P<0.05);添加0.08% TFE显著提高了盲肠厚壁菌门和乳酸菌属相对丰度(P<0.05)。综上所述,在饲粮中添加TFE可提高文昌鸡的生长性能,增强血清免疫和抗氧化功能,改善肠道黏膜形态,调节肠道菌群,缓解夏季湿热对机体的炎症损伤。在本试验条件下,基于回归模型分析,文昌鸡饲粮中TFE的适宜添加水平为0.067%。

关键词： 山香圆叶提取物; 湿热条件; 文昌鸡; 生长性能; 屠宰性能; 免疫和抗氧化功能; 肠道微生态

本文引用格式

宋文静 , 宋琼莉 , 陈小连 , 刘林秀 , 谭佳 , 邹志恒 , 魏立民 , 熊平文 , 张艳 , 孙瑞萍 . 山香圆叶提取物对湿热条件下文昌鸡生长性能、屠宰性能、血清免疫和抗氧化功能及肠道微生态的影响[J]. 动物营养学报, 2022 , 34(8) : 5048 -5062 . DOI: 10.3969/j.issn.1006-267x.2022.08.029

Abstract

The purpose of this study was to evaluate the effects of Turpiniae folium extract (TFE) supplementation on the growth performance, slaughter performance, serum immune and antioxidant function and intestinal microecology of Wenchang chicken under hot-humid condition in summer. A total 240 female Wenchang chicken at 84 days of age with similar body weight were randomly divided into 4 groups, and each group included 6 replicates with 10 birds per replicate. Birds received either a basal diet (control group) or the basal diet+0.20%, 0.40% or 0.80% TFE. The experiment period lasted for 35 days. Throughout the experiment period, birds were under hot-humid condition (average daily temperature was 28.26 to 34.80 ℃, average daily relative humidity was 67.00% to 87.58%). The results showed as follows: 1) with the TFE supplementation level increasing, the average daily gain (ADG) linearly (P=0.004) and quadratically (P=0.001) changed. Compared with the control group, feed to gain ratio (F/G) tended to decrease (0.05≤P<0.10). 2) Compared with the control group, serum total protein (TP), immunoglobulin M (IgM) and insulin (INS) contents tended to increase (0.05≤P<0.10). With the TFE supplementation level increasing, serum interleukin-10 (IL-10) content linearly (P=0.002) and quadratically (P=0.006) changed, but serum interleukin-1β (IL-1β) and interleukin 6 (IL-6) contents linearly (P=0.004, P=0.005) and quadratically (P=0.009, P=0.005) changed. Compared with the control group, dietary supplementation with 0.04% and 0.08% TFE significantly decreased the serum glucose (GLU), tumor necrosis factor-α (TNF-α), heat stress protein 70 (HSP70) and malondialdehyde (MDA) contents (P＜0.05), but significantly increased the contents of serum immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin 10 (IL-10), total antioxidant capacity (T-AOC), and activities of superoxide dismutase (SOD) and peroxidase (GSH-Px) (P<0.05). 3) With the TFE supplementation level increasing, spleen index quadratically changed (P=0.043). 4) Jejunum villus height (VH) and villus height/crypt depth (VH/CD) in 0.04% and 0.08% TFE groups were significantly higher than those in the control group (P<0.05). 5) With the TFE supplementation level increasing, the relative abundance of Proteobacteria linearly (P=0.016) and quadratically (P=0.011) changed. Compared with the control group, the relative abundance of Bifidobacterium tended to increased (0.05≤P<0.10). Compared with the control group, adding 0.04% and 0.08% TFE significantly decreased the relative abundance of Fusobacteria and Prevotellaceae_UCG-001 (P<0.05), and adding 0.08% TFE significantly increased the relative abundance of Firmicutes and Lactobacillus (P<0.05). In conclusion, TFE supplementation improves the growth performance, immune and antioxidant fuction of Wenchang chicken, alleviates inflammatory reaction, improves intestinal mucosal morphology and regulates intestinal microflora. According to the quadratic regression analyses fitted to significance parameters, the optimal dietary TFE supplementation level is 0.067%.

Key words： Turpinia folium extract; hot-humid condition; Wenchang chicken; growth performance; slaughter performance; immune and antioxidant function; intestinal microecology

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