动物营养学报 >

2022 , Vol. 34 >Issue 8: 5146 - 5155

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.038

研究论文 RESEARCH PAPER

饲粮添加丁酸梭菌对育肥山羊生长性能和直肠微生物区系的影响

  • 张城瑞 ,
  • 于清源 ,
  • 王季红 ,
  • 孙雨坤 ,
  • 宛涛 ,
  • 余奕东 ,
  • 张永根
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  • 1. 东北农业大学动物科学技术学院, 哈尔滨 150030;
    2. 内蒙古农业大学草原与资源环境学院, 呼和浩特 010010;
    3. 鄂尔多斯市农牧业科学研究院, 鄂尔多斯 017000
张城瑞(1997—),男,甘肃白银人,硕士研究生,从事反刍动物营养与饲料科学研究。E-mail:cruivan8254936@163.com

收稿日期: 2022-01-20

  网络出版日期: 2022-08-11

基金资助

财政部和农业农村部——国家现代农业产业技术体系资助;优质饲草料节水高效生产利用技术研究与示范(KJXM-EEDS-2020010-04);沙地生态系统近自然修复技术研究及产业化示范(2019ZD007)

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Effects of Dietary Supplementation with Clostridium butyricum on Growth Performance and Rectal Microflora of Fattening Goats

  • ZHANG Chengrui ,
  • YU Qingyuan ,
  • WANG Jihong ,
  • SUN Yukun ,
  • WAN Tao ,
  • YU Yidong ,
  • ZHANG Yonggen
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  • 1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
    2. College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, China;
    3. Ordos Academy of Agriculture and Animal Husbandry, Ordos 017000, China

Received date: 2022-01-20

  Online published: 2022-08-11

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摘要

本试验旨在探究饲粮添加丁酸梭菌对育肥山羊生长性能和直肠微生物区系的影响。试验选取体重[(22.00±2.09) kg]相近且健康的6月龄阿尔巴斯山羊16头,随机分为2组,每组8个重复,每个重复1头。对照组饲喂基础饲粮(精粗比=65：35),丁酸梭菌组饲喂在基础饲粮中添加2.0×108 CFU/kg丁酸梭菌(活菌数≥1.0×109 CFU/g)的试验饲粮。预试期14 d,正试期56 d。结果表明:与对照组相比,饲粮中添加丁酸梭菌对育肥山羊生长性能、养分表观消化率和氮代谢无显著影响(P＞0.05),但添加丁酸梭菌后显著增加血浆中葡萄糖(GLU)、总胆固醇(TC)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)的含量(P＜0.05);对于血浆中天冬氨酸氨基转移酶(AST)的活性以及总蛋白(TP)和球蛋白(GLB)的含量有提高的趋势(0.05＜P＜0.10)。与对照组相比,饲喂丁酸梭菌的育肥山羊直肠中克里斯滕森菌科_R-7群相对丰度显著升高(P＜0.05),而瘤胃菌科_UCG-014相对丰度显著下降(P＜0.05);直肠中梭菌属相对丰度呈降低趋势(0.05＜P＜0.10),而弯曲杆菌属相对丰度呈升高趋势(0.05＜P＜0.10)。综上所述,高精料饲粮中添加丁酸梭菌可提高山羊血浆中GLU和HDL的含量,并可通过上调有益菌的相对丰度,改善直肠微生物区系结构,但对育肥山羊的生长性能无显著影响。

关键词： 丁酸梭菌; 育肥山羊; 生长性能; 血浆生化指标; 直肠微生物区系

本文引用格式

张城瑞 , 于清源 , 王季红 , 孙雨坤 , 宛涛 , 余奕东 , 张永根 . 饲粮添加丁酸梭菌对育肥山羊生长性能和直肠微生物区系的影响[J]. 动物营养学报, 2022 , 34(8) : 5146 -5155 . DOI: 10.3969/j.issn.1006-267x.2022.08.038

Abstract

The purpose of this experiment was to investigate the effects of dietary Clostridium butyricum (C. butyricum) on growth performance and rectal microflora of fattening goats. A total of 16 healthy 6-month-old male Albas goats with similar body weight of (22.00±2.09) kg were selected and randomly divided into 2 groups with 8 replicates per group, and 1 goat per replicate: the control group was fed a basal diet (concentrate to forage ratio = 65：35); the C. butyricum group was fed the basal diet+2.0×108 CFU/kg C. butyricum (≥1.0×109 CFU/g). The pre-feeding period was 14 days, and the experimental period was 56 days. The results showed that compared with the control group, the supplementation of C. butyricum to the diet had no significant effects on the growth performance, nutrient apparent digestibility and nitrogen metabolism of fattening goats (P>0.05), but adding C. butyricum could significantly increase plasma glucose (GLU) and total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) contents (P<0.05). The activity of aspartate aminotransferase (AST) and the contents of total protein (TP) and globulin (GLB) in plasma showed an increasing trend (0.05<P<0.10). Compared with the control group, the relative abundance of Christensenaceae _R-7_group in the rectum of fattening goats fed with C. butyricum was significantly increased (P<0.05), while the relative abundance of Ruminobacteriaceae _UCG-014 was significantly decreased (P<0.05). The relative abundance of Clostridium in the rectum showed a decreasing trend (0.05＜P＜0.10), while the relative abundance of Campylobacter showed an increasing trend (0.05＜P＜0.10). In conclusion, high-concentrate diets supplementation with C. butyricum can increase the contents of GLU and HDL in goat plasma, and improve the rectal microflora structure by up-regulating the relative abundance of beneficial bacteria, but there is no significant effect on the growth performance of fattening goats.

Key words： Clostridium butyricum; fattening goats; growth performance; plasma biochemical indexes; rectal microflora

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