研究论文 RESEARCH PAPER

维生素A对哺乳期滩羔羊生长性能、抗氧化能力、血清脂质代谢指标及肌内脂肪沉积相关基因表达的影响

  • 李海海 ,
  • 张腱皓 ,
  • 魏大为 ,
  • 寇启芳 ,
  • 张桂杰
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  • 1. 宁夏大学农学院动物科学系, 银川 750021;
    2. 红寺堡区天源良种羊繁育养殖有限公司, 吴忠 751900
李海海(1995—),男,山东菏泽人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:2601931502@qq.com

收稿日期: 2022-03-03

  网络出版日期: 2022-08-11

基金资助

国家自然科学基金项目(31960672);滩羊营养需要标准制定及高效转化技术研究项目(2021BBF02034);宁夏高等学校一流学科建设(草学学科)资助项目(NXYLXK2017A01)

Effects of Vitamin A on Growth Performance, Antioxidant Capacity, Serum Lipid Metabolism Indices and Expression of Genes Related to Intramuscular Fat Deposition of Lactating Tan Lambs

  • LI Haihai ,
  • ZHANG Jianhao ,
  • WEI Dawei ,
  • KOU Qifang ,
  • ZHANG Guijie
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  • 1. Department of Animal Science, School of Agriculture, Ningxia University, Yinchuan 750021, China;
    2. Hongsibu District Tianyuan Well-Bred Sheep Breeding Co., Ltd., Wuzhong 751900, China

Received date: 2022-03-03

  Online published: 2022-08-11

摘要

本试验旨在探究肌内脂肪生成关键窗口期补饲维生素A对哺乳期滩羔羊生长性能、抗氧化能力、血清脂质代谢指标及肌内脂肪沉积相关基因表达的影响。选用健康、胎次和体重[(46.83±1.30) kg]相近的经产滩羊母羊60只,进行同期发情和人工授精处理。分娩后选择出生日期、体重[(4.38±0.23) kg]相近的健康滩羔羊45只,随机分为3个组,每组3个重复(圈),每个重复5只羔羊。在正常哺乳的基础上,3组羔羊在1日龄和1月龄分别灌服0(CON组)、30 000(LVA组)和60 000 IU/只(HVA组)维生素A。试验期70 d。结果表明,与CON组相比:1) LVA组和HVA组羔羊断奶重显著提高(P<0.05);2) LVA组和HVA组血清视黄酸含量显著提高(P<0.05),HVA组血清视黄醇含量显著降低(P<0.05);3) LVA组背最长肌锌指蛋白423(ZFP423)和过氧化物酶体增殖物激活受体γ(PPARγ) mRNA相对表达量显著提高(P<0.05),HVA组背最长肌ZFP423 mRNA相对表达量显著提高(P<0.05);4) LVA组和HVA组血清总胆固醇、甘油三酯和低密度脂蛋白含量显著提高(P<0.05);5) LVA组和HVA组血清总抗氧化能力和超氧化物歧化酶活性显著提高(P<0.05)、血清丙二醛含量显著降低(P<0.05),LVA组血清谷胱甘肽过氧化物酶活性显著提高(P<0.05)。综上所述,维生素A能够提高哺乳期滩羔羊生长性能及抗氧化能力,提高背最长肌脂肪沉积相关基因表达,并对血清脂质代谢指标有一定的影响,建议添加量为30 000 IU。

本文引用格式

李海海 , 张腱皓 , 魏大为 , 寇启芳 , 张桂杰 . 维生素A对哺乳期滩羔羊生长性能、抗氧化能力、血清脂质代谢指标及肌内脂肪沉积相关基因表达的影响[J]. 动物营养学报, 2022 , 34(8) : 5234 -5243 . DOI: 10.3969/j.issn.1006-267x.2022.08.045

Abstract

This experiment was conducted to investigate the effects of vitamin A supplementation on the growth performance, antioxidant capacity, serum lipid metabolism indices and gene expression related to intramuscular fat deposition of lactating Tan lambs during the critical window period of intramuscular adipogenesis. Sixty Tan sheep with similar health, parity and body weight [(46.83±1.30) kg] were selected for simultaneous estrus and artificial insemination. After delivery, 45 healthy Tan lambs with similar birth date and weight [(4.38±0.23) kg] were selected and randomly divided into 3 groups, each group had 3 repetitions (pens), and each repetition had 5 lambs. In addition to normal lactation, these lambs were fed with 0 (CON group), 30 000 (LVA group) and 60 000 IU per lamb (HVA group) vitamin A at 1 day and 1 month of age, respectively. The experimental period was 70 days. The results showed that compared with the control group: 1) the weaning weight of lambs in LVA and HVA groups was significantly increased (P<0.05); 2) the serum retinoic acid content in LVA and HVA groups was significantly increased (P<0.05), while the serum retinol content in HVA group was significantly decreased (P<0.05); 3) the mRNA relative expression levels of zinc finger protein 423 (ZFP423) and peroxisome proliferator-activated receptor γ (PPARγ) in longissimus dorsi in LVA group was significantly increased (P<0.05), and the ZFP423 mRNA relative expression level in longissimus dorsi in HVA group was significantly increased (P<0.05); 4) the serum contents of total cholesterol, triglyceride and low density lipoprotein in LVA and HVA groups were significantly increased (P<0.05); 5) the serum total antioxidant capacity and superoxide dismutase activity in LVA and HVA groups were significantly increased (P<0.05), but the serum malondialdehyde content was significantly decreased (P<0.05), and the serum glutathione peroxidase activity in LVA group was significantly increased (P<0.05). In conclusion, vitamin A can increase the growth performance and antioxidant capacity of Tan lambs during lactation, increase the expression of fat deposition-related genes in longissimus dorsi, and have some effects on serum lipid metabolism indexes. Therefore, the appropriate dosage of vitamin A for Tan lambs is 30 000 IU.

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