研究论文 RESEARCH PAPER

饲料添加丁酸梭菌对黄颡鱼生长性能及血清生化指标、免疫功能和抗氧化能力的影响

  • 王海瑞 ,
  • 莫文艳 ,
  • 赵红霞 ,
  • 黄文 ,
  • 郑春田 ,
  • 曹俊明
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  • 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640
王海瑞(1995—),男,山西运城人,硕士研究生,从事水产动物营养与饲料研究。E-mail:1346612369@qq.com

收稿日期: 2022-01-20

  网络出版日期: 2022-08-11

基金资助

国家自然科学基金项目(31402307,31902388);广东省自然科学基金项目(2021A1515010850)

Effects of Adding Clostridium butyricum in Diets on Growth Performance, Serum Biochemical Indices, Immune Function and Antioxidant Ability of Yellow Catfish (Pelteobagrus fulvidraco)

  • WANG Hairui ,
  • MO Wenyan ,
  • ZHAO Hongxia ,
  • HUANG Wen ,
  • ZHENG Chuntian ,
  • CAO Junming
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  • Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2022-01-20

  Online published: 2022-08-11

摘要

本试验旨在研究饲料中添加丁酸梭菌对黄颡鱼(Pelteobagrus fulvidraco)生长性能及血清生化指标、免疫功能和抗氧化能力的影响。选取初始体重为(5.55±0.05) g的黄颡鱼450尾,随机分成5组,每组3个重复,每个重复30尾。在基础饲料中分别添加0、2、20、200、2 000 mg/kg丁酸梭菌配制5种试验饲料,分别记作G0、G1、G2、G3、G4,饲料中丁酸梭菌数量分别为0、4.8×106、4.5×107、5.1×108、3.6×109 CFU/kg,饲养周期为56 d。结果表明:1)与G0组比较,G1、G2和G3组黄颡鱼的增重率显著增加(P<0.05),饲料系数却显著降低(P<0.05)。与G0组相比,G1、G2、G3和G4组黄颡鱼的脏体比显著降低(P<0.05),G2、G3组黄颡鱼的肥满度显著升高(P<0.05)。2) G3、G4组黄颡鱼血清谷丙转氨酶活性显著低于G0组(P<0.05),G3组黄颡鱼血清谷草转氨酶活性显著低于G0组(P<0.05)。G1、G2和G3组黄颡鱼血清葡萄糖含量显著高于G0组(P<0.05)。3)与G0组相比,G3组黄颡鱼血清的谷胱甘肽含量及溶菌酶和超氧化物歧化酶活性显著升高(P<0.05),G4组黄颡鱼血清中补体3含量和碱性磷酸酶活性显著增加(P<0.05),G3、G4组黄颡鱼血清免疫球蛋白M含量显著提高(P<0.05)。G1、G2、G3和G4组黄颡鱼血清总抗氧化能力显著高于G0组(P<0.05)。4)与G0组相比,G2、G3和G4组肠道短链脂肪酸含量显著升高(P<0.05)。综上所述,以生长性能、血清免疫和抗氧化指标为评价标准,建议黄颡鱼饲料中丁酸梭菌的添加水平为200 mg/kg (5.1×108 CFU/kg)。

本文引用格式

王海瑞 , 莫文艳 , 赵红霞 , 黄文 , 郑春田 , 曹俊明 . 饲料添加丁酸梭菌对黄颡鱼生长性能及血清生化指标、免疫功能和抗氧化能力的影响[J]. 动物营养学报, 2022 , 34(8) : 5295 -5303 . DOI: 10.3969/j.issn.1006-267x.2022.08.050

Abstract

This experiment was aimed to study the effects of adding Clostridium butyricum in diets on growth performance, serum biochemical indices, immune function and antioxidant ability of yellow catfish (Pelteobagrus fulvidraco). A total of 450 yellow catfish with an initial body weight of (5.55±0.05) g were randomly divided into 5 groups with 3 replicates per group and 30 fish per replicate. Five experimental diets were prepared by adding 0, 2, 20, 200 and 2 000 mg/kg Clostridium butyricum into the basal diet, named as G0, G1, G2, G3 and G4, respectively. The number of Clostridium butyricum in the diets was 0, 4.8×106, 4.5×107, 5.1×108 and 3.6×109 CFU/kg, respectively. The feeding period was 56 days. Results showed as follows: 1) compared with G0 group, the weight gain rate of yellow catfish in G1, G2 and G3 groups was significantly increased (P<0.05), but the feed conversion ratio was significantly decreased (P<0.05). 2) The serum glutamic-pyruvic transaminase activity in G3 and G4 groups was significantly lower than that in G0 group (P<0.05), and the serum glutamic-pyruvic transaminase activity in G3 group was significantly lower than that in G0 group (P<0.05). Serum glucose content of yellow catfish in G1, G2 and G3 groups was significantly higher than that in G0 group (P<0.05). 3) Compared with G0 group, the serum glutathione, lysozyme and superoxide dismutase activities in G3 group were significantly increased (P<0.05), the serum complement 3 content and alkaline phosphatase activity in G4 group were significantly increased (P<0.05), and the serum immunoglobulin M content in G3 and G4 groups was significantly increased (P<0.05). The serum total antioxidant capacity of yellow catfish in Clostridium butyricum group was significantly higher than that in G0 group (P<0.05). 4) Compared with G0 group, intestinal short-chain fatty acid content in G2, G3 and G4 groups was significantly increased (P<0.05). In conclusion, it is recommended that the supplemental level of Clostridium butyricum is 200 mg/kg (5.1×108 CFU/kg) based on growth performance, serum immune and antioxidant indexes as evaluation criteria.

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