研究论文 RESEARCH PAPER

饲料添加L-精氨酸或N-氨甲酰谷氨酸对杂交鳢生长性能、血浆生化指标、肠道功能及抗氧化能力的影响

  • 李培佳 ,
  • 侯冬强 ,
  • 赵红霞 ,
  • 陈冰 ,
  • 彭凯 ,
  • 黄文 ,
  • 郑春田 ,
  • 曹俊明
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  • 1. 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
    2. 广东海洋大学水产学院, 湛江 524088;
    3. 广州飞禧特生物科技有限公司, 广州 510640
李培佳(1998—),男,河南鹤壁人,硕士研究生,从事水产动物营养与饲料研究。E-mail:2633134605@qq.com

收稿日期: 2022-02-28

  网络出版日期: 2022-08-11

基金资助

国家自然科学基金项目(31402307,31902388);广东省自然科学基金项目(2021A1515010850)

Effects of Dietary L-Arginine or N-Carbamoylglutamate on Growth Performance, Plasma Biochemical Indices, Intestinal Function and Antioxidant Ability of Hybrid Snakehead (Channa maculata ♀×Channa argus♂)

  • LI Peijia ,
  • HOU Dongqiang ,
  • ZHAO Hongxia ,
  • CHEN Bing ,
  • PENG Kai ,
  • HUANG Wen ,
  • ZHENG Chuntian ,
  • CAO Junming
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  • 1. Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China;
    3. Guangzhou Fishtech Biotechnology Co., Ltd., Guangzhou 510640, China

Received date: 2022-02-28

  Online published: 2022-08-11

摘要

本试验旨在研究饲料中添加L-精氨酸(Arg)或N-氨甲酰谷氨酸(NCG)对杂交鳢(Channa maculata ♀×Channa argus ♂)幼鱼生长性能、血浆生化指标、肠道功能和抗氧化能力的影响。试验选取初始质量为(22.02±0.02) g的杂交鳢450尾,随机分为3组,每组3个重复,每个重复50尾。3个组分别投喂添加0(对照)、0.60% Arg、0.03% NCG的3种试验饲料,饲喂8周。结果表明:与对照组相比,饲料中添加0.60% Arg显著提高了杂交鳢增重率、特定生长率(P<0.05),添加0.03% NCG显著提高了杂交鳢存活率(P<0.05),添加0.60% Arg或0.03% NCG显著提高了杂交鳢的蛋白质沉积率、全鱼粗蛋白质含量(P<0.05),显著降低了饲料系数(P<0.05)。各组间杂交鳢的全鱼水分、粗脂肪、粗灰分含量没有显著差异(P>0.05)。饲料中添加0.60% Arg或0.03% NCG对杂交鳢血浆总蛋白、甘油三酯、葡萄糖、尿素氮、总胆固醇含量及血浆谷草转氨酶、谷丙转氨酶及肠道Na+/K+ATP酶活性无显著影响(P>0.05)。与对照组相比,饲料中添加0.03% NCG显著提高了杂交鳢肠道淀粉酶活性(P<0.05),饲料中添加0.60% Arg显著提高了杂交鳢肠道蛋白酶、脂肪酶和γ-谷氨酰基转移酶活性(P<0.05)。与对照组相比,饲料中添加0.60% Arg显著增强了杂交鳢血浆总抗氧化能力(P<0.05),添加0.03% NCG显著增强了杂交鳢血浆过氧化氢酶和过氧化物酶活性(P<0.05)。饲料中添加0.60% Arg或0.03% NCG对杂交鳢血浆超氧化物歧化酶、谷胱甘肽过氧化物酶活性及丙二醛含量无显著影响(P>0.05)。综上所述,饲料中添加Arg能够通过提高肠道消化酶活性,改善杂交鳢生长性能,而添加NCG可能通过促进抗氧化能力提高杂交鳢成活率。

本文引用格式

李培佳 , 侯冬强 , 赵红霞 , 陈冰 , 彭凯 , 黄文 , 郑春田 , 曹俊明 . 饲料添加L-精氨酸或N-氨甲酰谷氨酸对杂交鳢生长性能、血浆生化指标、肠道功能及抗氧化能力的影响[J]. 动物营养学报, 2022 , 34(8) : 5304 -5312 . DOI: 10.3969/j.issn.1006-267x.2022.08.051

Abstract

This experiment was conducted to investigate the effects of dietary L-arginine (Arg) or N-carbamylglutamate (NCG) on growth performance, plasma biochemical indices, intestinal function and antioxidant ability of hybrid snakehead (Channa maculata♀×Channa argus ♂). Four hundred and fifty hybrid snakehead with an initial weight of (22.02±0.02) g were randomly divided into 3 groups with 3 replicates per group and 50 fish per replicate. Hybrid snakeheads were fed 3 experimental diets supplemented with 0 (control), 0.60% Arg and 0.03% NCG for 8 weeks, respectively. The results showed that compared with the control group, the addition of 0.60% Arg to the diet significantly increased the weight gain rate (WGR) and specific growth rate (SGR) of the hybrid snakeheads (P<0.05), and the addition of 0.03% NCG significantly increased the survival rate (SR) of the hybrid snakeheads (P<0.05), adding 0.60% Arg or 0.03% NCG significantly increased protein deposition rate (PDR) and whole body crude protein content (P<0.05), and significantly decreased feed conversion ratio (FCR) (P<0.05). There were no significant differences in water, crude fat and ash contents of whole fish among all groups (P>0.05). Diet supplemented with 0.60% Arg or 0.03% NCG had no significant effects on plasma contents of total protein, triglyceridin, glucose, urea nitrogen, total cholesterol, and the plasma activities of glutamic-oxalacetic transaminase, glutamic-pyruvic transaminase and intestinal Na+/K+ATPase (P>0.05). Compared with the control group, the addition of 0.03% NCG to the diet significantly increased the intestinal amylase activity of the hybrid snakeheads (P<0.05), and the addition of 0.60% Arg to the diet significantly increased the intestinal protease, lipase and γ-glutamyl transferase activities of the hybrid snakeheads (P<0.05). Compared with control group, 0.60% Arg supplementation significantly increased plasma total antioxidant capacity (P<0.05), and 0.03% NCG supplementation significantly increased plasma catalase and peroxidase activities (P<0.05). Diet supplemented with 0.60% Arg or 0.03% NCG had no significant effects on plasma superoxide dismutase (SOD) and glutathione peroxidase (GSH) activities and malondialdehyde (MDA) content (P>0.05). In conclusion, dietary Arg can improve the growth performance of hybrid snakeheads by improving the activities of intestinal digestive enzymes, while dietary NCG may improve the survival rate of hybrid snakeheads by promoting antioxidant ability.

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