动物营养学报 >

2022 , Vol. 34 >Issue 8: 5393 - 5403

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.059

研究论文 RESEARCH PAPER

岩藻多糖对大鼠肾脏氧化应激、炎症和细胞凋亡的缓解作用

  • 蒯云逸 ,
  • 蒋和浩 ,
  • 红雷 ,
  • 徐元庆
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  • 内蒙古农业大学动物科学学院, 呼和浩特 010018
蒯云逸(1998—),男,山西朔州人,本科生,研究方向为动物营养与饲料科学。E-mail:kuaiyunyi@emails.imau.edu.cn

收稿日期: 2022-01-26

  网络出版日期: 2022-08-11

基金资助

内蒙古农业大学高层次人才引进科研启动项目(NDYB2018-25);内蒙古农业大学动物科学学院青年基金(QN201924)

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Alleviating Effects of Fucoidan on Oxidative Stress, Inflammation and Apoptosis in Kidney of Rats

  • KUAI Yunyi ,
  • JIANG Hehao ,
  • HONG Lei ,
  • XU Yuanqing
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  • Collage of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2022-01-26

  Online published: 2022-08-11

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摘要

为了探讨岩藻多糖对大鼠肾脏氧化损伤的缓解作用,选取28只8周龄SD雄性大鼠,随机分为3个组,分别为对照组(n=10)、氧化应激组(n=9)以及岩藻多糖与氧化应激联合处理组(n=9)。对照组和氧化应激组饲喂基础饲粮,岩藻多糖与氧化应激联合处理组饲喂在基础饲粮中添加250 mg/kg岩藻多糖的饲粮。试验期20 d。于试验的第12天,氧化应激组和岩藻多糖与氧化应激联合处理组通过腹腔注射10 mg/kg BW的Diquat,建立氧化应激模型;对照组注射等量的生理盐水。之后继续饲喂8 d后采集大鼠血液和肾脏组织样品。结果表明:饲喂岩藻多糖能够降低氧化应激诱导的大鼠血清尿素氮(P=0.061)和肌酐(P<0.05)以及肾脏丙二醛(P＝0.052)含量的升高,并逆转由氧化应激诱导的肾脏过氧化氢酶(CAT)(P=0.064)、超氧化物歧化酶2(SOD2)(P<0.05)及核因子E2相关因子2(Nrf2)(P<0.01)基因表达的下调,从而提高肾脏CAT (P<0.05)、超氧化物歧化酶(SOD)(P=0.068)和谷胱甘肽过氧化物酶(GPx)(P=0.085)的活性。此外,饲喂岩藻多糖下调了氧化应激诱导的大鼠肾脏白细胞介素-1(IL-1)(P<0.01)、肿瘤坏死因子-α(TNF-α)(P=0.079)、核转录因子-κB (NF-κB)(P<0.05)、B淋巴细胞瘤/白血病-2相关X蛋白(Bax)(P<0.01)、半胱氨酸天冬氨酸蛋白酶-8(Caspase-8)(P<0.01)、凋亡相关因子(Fas)(P<0.01)和凋亡相关因子配体(FasL)(P=0.064)基因表达的上升,并上调了肾脏B淋巴细胞瘤/白血病-2(Bcl-2)的基因表达及Bcl-2与Bax基因表达的比值(P<0.01)。总体而言,岩藻多糖能够通过其抗氧化、抗炎及抗细胞凋亡活性缓解大鼠肾脏组织的氧化损伤。

关键词： 岩藻多糖; 肾脏; 氧化应激; 细胞凋亡

本文引用格式

蒯云逸 , 蒋和浩 , 红雷 , 徐元庆 . 岩藻多糖对大鼠肾脏氧化应激、炎症和细胞凋亡的缓解作用[J]. 动物营养学报, 2022 , 34(8) : 5393 -5403 . DOI: 10.3969/j.issn.1006-267x.2022.08.059

Abstract

This study was conducted to investigate the alleviating effects of fucoidan on kidney oxidative damage of rats. A total of 28 SD rats of 8-week-old were randomly divided into 3 groups as control group (n=10), oxidative stress group (n=9) and fucoidan combined with oxidative stress treatment group (n=9). Rats in the control group and the oxidative stress group were fed a basal diet, and those in the fucoidan combined with oxidative stress treatment group were fed the basal diet supplemented with 250 mg/kg fucoidan. The experiment lasted for 20 days. On the 12th day of the experiment, the oxidative stress group and the fucoidan combined with oxidative stress treatment group were injected intraperitoneally with 10 mg/kg BW Diquat to establish the oxidative stress model, and the control group was injected with the same amount of normal saline. After the rats were fed for another 8 days, the blood and kidney tissue samples were collected. The results showed that dietary fucoidan reduced the increase of serum contents of urea nitrogen (P=0.061) and creatinine (P<0.05) as well as the kidney malondialdehyde content (P=0.052) of rats induced by oxidative stress, and increased the activities of catalase (CAT) (P<0.05), superoxide dismutase (SOD) (P=0.068) and glutathione peroxidase (GPx) (P=0.085) in kidney by reversing the downregulation of CAT (P=0.064), superoxide dismutase 2 (SOD2) (P<0.05) and nuclear factor E2 related factor 2 (Nrf2) (P<0.01) gene expression induced by oxidative stress in kidney. In addition, dietary fucoidan down-regulated the increasing gene expression of interleukin-1 (IL-1) (P<0.01), tumor necrosis factor-α (TNF-α) (P=0.079), nuclear factor-κB (NF-κB) (P<0.05), B-cell lymphoma/leukemia-2-associated X protein (Bax) (P<0.01), Caspase-8 (P<0.01), factor associated suicide (Fas) (P<0.01) and factor associated suicide ligand (FasL) (P=0.064) in kidney of rats, and up-regulated the B-cell lymphoma/leukemia-2 (Bcl-2) gene expression and increased the ratio of Bcl-2 to Bax gene expression in kidney (P<0.01). In conclusion, fucoidan can alleviate the oxidative damage of kidney tissues of rats through its antioxidant, anti-inflammatory and anti-apoptotic activities.

Key words： fucoidan; kidney; oxidative stress; apoptosis

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