研究论文 RESEARCH PAPER

类肠膜魏斯氏菌对猪生长性能、粪菌和代谢产物的影响

  • 刘韶娜 ,
  • 赵彦光 ,
  • 张斌 ,
  • 相德才 ,
  • 赵智勇
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  • 云南省畜牧兽医科学院, 昆明 650224
刘韶娜(1983—),女,山东招远人,副研究员,硕士,主要从事动物营养与饲料科学及肠道微生物学的研究。E-mail:289066103@qq.com

收稿日期: 2022-03-31

  网络出版日期: 2022-10-17

基金资助

云南省重大科技专项计划(202102AE090039);云南省重点研发计划项目(2018BB003)

Effects of Weissella paramesenteroides on Growth Performance, Fecal Microbiota and Metabolite in Pigs

  • LIU Shaona ,
  • ZHAO Yanguang ,
  • ZHANG Bin ,
  • XIANG Decai ,
  • ZHAO Zhiyong
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  • Yunnan Animal Science and Veterinary Institute,Kunming 650224, China

Received date: 2022-03-31

  Online published: 2022-10-17

摘要

本试验旨在研究类肠膜魏斯氏菌对猪生长性能、粪菌和代谢产物的影响。选用(228±2)日龄、体重为(95.84±10.05) kg的迪庆藏猪选育猪20头,随机分为2组,每组2个重复,每个重复5头。对照组饲喂基础饲粮,试验组饲喂在基础饲粮中添加类肠膜魏斯氏菌(107 CFU/g)的试验饲粮。预试期7 d,正试期22 d。结果表明:1)2组生长性能无显著差异(P>0.05)。2)2组粪菌的α多样性无显著差异(P>0.05)。门水平上,试验组的螺旋体门和变形菌门相对丰度显著或极显著高于对照组(P<0.05或P<0.01);属水平上,狭义梭菌属_1和密螺旋体属_2的相对丰度显著或极显著高于对照组(P<0.05或P<0.01),norank_f_F082和瘤胃球菌科_UCG-002相对丰度则显著低于对照组(P<0.05)。多级物种差异判别分析(LEfSe)获得27个生物标记物(LDA值>3.0),其中试验组获得生物标记物15个。对照组LDA值最大的是瘤胃球菌科_UCG-002,试验组LDA值最大的是狭义梭菌属_1。3)偏最小二乘法判别分析(PLS-DA)发现,正负离子模式下,样品完全分开,试验组2-羟基十六烷酸、N-di-腺嘌呤脱氧核苷酸、2-羟基十四烷酸和Avocadene acetate的差异表达倍数显著或极显著上调(P<0.05或P<0.01),Corchorifatty acid F和2,3-丁炔前列腺素E1差异表达倍数显著或极显著下调(P<0.05或P<0.01),试验组2-羟基十四烷酸和Corchorifatty acid F分别是上调和下调差异倍数最高的代谢产物。综上所述,添加类肠膜魏斯氏菌能改变猪粪便的菌群结构,显著提高短链脂肪酸产生菌(狭义梭菌属_1)的相对丰度,提高脂肪酸类物质的差异表达倍数(2-羟基十六烷酸、2-羟基十四烷酸和Avocadene acetate),降低2,3-丁炔前列腺素E1的差异表达倍数,具有潜在的益生功能。

本文引用格式

刘韶娜 , 赵彦光 , 张斌 , 相德才 , 赵智勇 . 类肠膜魏斯氏菌对猪生长性能、粪菌和代谢产物的影响[J]. 动物营养学报, 2022 , 34(9) : 5597 -5608 . DOI: 10.3969/j.issn.1006-267x.2022.09.016

Abstract

The study aimed to explore the effects of the Weissella paramesenteroides on the growth performance, fecal microbiota and metabolite in pigs. Twenty Diqing Tibetan pig breeding pigs at (228±2) days of age and with body weight of (95.84±10.05) kg were randomly divided into 2 groups with two repetitions in each group and 5 pigs in each repetition. The pigs in control group were fed a basal diet, and the pigs in experimental group were fed the basal diet supplemented with Weissella paramesenteroides (107 CFU/g), respectively. The experimental period was 22 days with 7 day pre-experiment. The results showed as follows:1) there is no significant difference between the two groups in growth performance (P>0.05). 2) The alpha diversity of 2 groups had no significant difference (P>0.05). At the phylum level, the relative abundance of Spirochaetes (P<0.01) and Proteobacteria (P<0.05) in experimental group were significantly higher than those in the control group. At the genus level, the relative abundance of Clostridium_sensu_stricto_1 (P<0.05) and Treponema_2 (P<0.01) in experimental group was significantly higher than thoese in the control group, the relative abundance of norank_f_F082 and Ruminococcaceae_UCG-002 was significantly lower than those in the control group (P<0.05). When the LDA value was higher than 3.0, 27 biomarkers were obtained, while experimental group was obtained 15 biomarkers. Clostridium_sensu_stricto_1 was the most significant in experimental group, and that in control group was Ruminococcaceae_UCG-002. 3) Partial least squares discrimination analysis (PLS-DA) showed that the two groups were separated in positive and negative model, the metabolite analysis showed that the differential expression multiples of 2-hydroxyhexadecanoic acid, N-di-deoxyadenosine monophosphate, 2-hydroxymyristic acid and avocadene acetate were up-regulated (P<0.05 or P<0.01), those of 2 3-dinor prostaglandin E1 and corchorifatty acid F were down-regulated significantly in experimental group (P<0.05 or P<0.01), those of 2-hydroxymyristic acid and corchorifatty acid F were the most significant, respectively. In summary, the addition of Weissella paramesenteroides can optimize the structure of fecal microbiota in pigs, improve the relative abundance of Clostridium_sensu_stricto_1 which can produce the short chain fatty, improve the differential expression multiples of 2-hydroxyhexadecanoic acid, 2-hydroxymyristic acid and avocadene acetate which belongs to fatty acid substances, reduces the differential expression multiples of 2,3-dinor prostaglandin E1. It has the potential function of health-keeping and metabolism-promoting.

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