研究论文 RESEARCH PAPER

发酵桑叶对肥育猪生长性能以及血浆、尿液代谢物的影响

  • 崔艺燕 ,
  • 彭苏 ,
  • 李贞明 ,
  • 宋敏 ,
  • 余苗 ,
  • 邓盾 ,
  • 田志梅 ,
  • 容庭 ,
  • 刘志昌 ,
  • 马现永
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  • 1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
    2. 岭南现代农业科学与技术广东省实验室茂名分中心, 茂名 525000
崔艺燕(1987—),女,广东佛山人,助理研究员,硕士,从事动物营养与饲料科学、生态养殖与环境控制的研究。E-mail:958117076@qq.com

收稿日期: 2022-03-23

  网络出版日期: 2022-10-17

基金资助

广东省重点领域研发计划项目(2020B020225005);广州市农村科技特派员项目(20212100077);省级农业科技创新推广及农业资源与生态环境保护建设项目(2021KJ266);广东省农业科学院学科团队建设项目(202118TD);饲料产业技术体系创新团队建设项目(2022KJ115);茂名实验室自主科研项目(2021ZZ003);韶关市科技计划项目(200731114530834)

Effects of Fermented Mulberry Leaves on Growth Performance, Plasma and Urine Metabolites of Finishing Pigs

  • CUI Yiyan ,
  • PENG Su ,
  • LI Zhenming ,
  • SONG Min ,
  • YU Miao ,
  • DENG Dun ,
  • TIAN Zhimei ,
  • RONG Ting ,
  • LIU Zhichang ,
  • MA Xianyong
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  • 1. Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, The Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China

Received date: 2022-03-23

  Online published: 2022-10-17

摘要

本试验旨在研究饲粮中添加发酵桑叶对肥育猪生长性能以及血浆、尿液代谢物的影响。选取18头体重(78.19±2.05) kg的杜×长×大阉公猪,随机分为2组,每组9个重复,每个重复1头猪。对照组饲喂基础饲粮,发酵桑叶组在基础饲粮中添加10%发酵桑叶。试验期69 d。结果表明:1)与对照组相比,饲粮中添加发酵桑叶对肥育猪生长性能无显著影响(P>0.05)。2)与对照组相比,发酵桑叶组血浆肌酐、尿酸、甘油三酯含量和乳酸脱氢酶活性显著降低(P<0.05),血浆谷草转氨酶活性有降低趋势(P=0.079)。3)与对照组相比,发酵桑叶组尿液肌酐含量显著提高(P<0.05),尿液尿酸含量和乳酸脱氢酶活性显著降低(P<0.05)。4)与对照组相比,发酵桑叶组血浆谷胱甘肽含量显著提高(P<0.05)。5)与对照组相比,发酵桑叶组血浆白细胞介素(IL)-2、IL-6、IL-8、IL-22、肿瘤坏死因子-α、干扰素-γ、免疫球蛋白(Ig) A含量显著提高(P<0.05),血浆IgG和IgM含量有提高趋势(P=0.084和P=0.068)。由此可见,饲粮中添加10%发酵桑叶对肥育猪生长性能无负面影响,改善了血浆和尿液代谢物含量,提高了机体抗氧化能力和免疫功能。

本文引用格式

崔艺燕 , 彭苏 , 李贞明 , 宋敏 , 余苗 , 邓盾 , 田志梅 , 容庭 , 刘志昌 , 马现永 . 发酵桑叶对肥育猪生长性能以及血浆、尿液代谢物的影响[J]. 动物营养学报, 2022 , 34(9) : 5620 -5629 . DOI: 10.3969/j.issn.1006-267x.2022.09.018

Abstract

This experiment aimed to investigate the effects of fermented mulberry leaves on growth performance, plasma and urine metabolites of finishing pigs. Eighteen with body weight of (78.19±2.05) kg Duroc×Landrace×Large White barrow pigs were randomly assigned to 2 groups with 9 replicates per group and 1 pig per replicate. Pigs in the control group were fed a basal diet, and pigs in fermented mulberry leaves group were fed the basal diet supplemented with 10% fermented mulberry leaves. The experiment lasted for 69 days. The results showed as follows:1) compared with the control group, dietary fermented mulberry leaves had no significant effect on growth performance of finishing pigs (P>0.05). 2) Compared with the control group, the creatinine, uric acid, triglyceride contents and lactate dehydrogenase activity in plasma of fermented mulberry leaves group were significantly decreased (P<0.05), and the plasma glutamic oxaloacetic transaminase activity tended to decrease (P=0.079). 3) Compared with the control group, the urine creatinine content of fermented mulberry leaves group was significantly increased (P<0.05), while the uric acid content and lactate dehydrogenase activity in urine were significantly decreased (P<0.05). 4) Compared with the control group, the plasma glutathione content of fermented mulberry leaves group was significantly increased (P<0.05). 5) Compared with the control group, the contents of interleukin (IL)-2, IL-6, IL-8, IL-22, tumor necrosis factor-α, interferon-γ and immunoglobulin (Ig) A in plasma of fermented mulberry leaves group were significantly increased (P<0.05), and the contents of IgG and IgM in plasma tended to increase (P=0.084 and P=0.068). In conclusion, dietary 10% fermented mulberry leaves have no negative effect on the growth performance, while improve the plasma and urine metabolite contents, and enhance the antioxidant capacity and immune function of finishing pigs.

参考文献

[1] HASSAN F U, ARSHAD M A, LI M W, et al.Potential of mulberry leaf biomass and its flavonoids to improve production and health in ruminants:mechanistic insights and prospects[J].Animals, 2020, 10(11):2076.
[2] MA G Q, CHAI X Y, HOU G G, et al.Phytochemistry, bioactivities and future prospects of mulberry leaves:a review[J].Food Chemistry, 2022, 372:131335.
[3] HUSSAIN F, RANA Z, SHAFIQUE H, et al.Phytopharmacological potential of different species of Morus alba and their bioactive phytochemicals:a review[J].Asian Pacific Journal of Tropical Biomedicine, 2017, 7(10):950-956.  
[4] YU Y F, ZHANG B, XIA Y H, et al.Bioaccessibility and transformation pathways of phenolic compounds in processed mulberry (Morus alba L.) leaves after in vitro gastrointestinal digestion and faecal fermentation[J].Journal of Functional Foods, 2019, 60:103406.
[5] LIU Y Y, LI Y H, XIAO Y, et al.Mulberry leaf powder regulates antioxidative capacity and lipid metabolism in finishing pigs[J].Animal Nutrition, 2021, 7(2):421-429.  
[6] ZENG Z, JIANG J J, YU J, et al.Effect of dietary supplementation with mulberry (Morus alba L.) leaves on the growth performance, meat quality and antioxidative capacity of finishing pigs[J].Journal of Integrative Agriculture, 2019, 18(1):143-151.  
[7] CHEN Z J, XIE Y Q, LUO J Y, et al.Dietary supplementation with Moringa oleifera and mulberry leaf affects pork quality from finishing pigs[J].Journal of Animal Physiology and Animal Nutrition, 2021, 105(1):72-79.  
[8] ZHAO X J, YANG R L, BI Y H, et al.Effects of dietary supplementation with mulberry (Morus alba L.) leaf polysaccharides on immune parameters of weanling pigs[J].Animals, 2019, 10(1):35.
[9] FAN L J, PENG Y, WU D, et al.Morus nigra L. leaves improve the meat quality in finishing pigs[J].Journal of Animal Physiology and Animal Nutrition, 2020, 104(6):1904-1911.  
[10] SUN H, LUO Y, ZHAO F F, et al.The effect of replacing wildrye hay with mulberry leaves on the growth performance, blood metabolites, and carcass characteristics of sheep[J].Animals, 2020, 10(11):2018.
[11] WANG B, LUO H L.Effects of mulberry leaf silage on antioxidant and immunomodulatory activity and rumen bacterial community of lambs[J].BMC Microbiology, 2021, 21(1):250.
[12] ZHOU Z M, ZHOU B, REN L P, et al.Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on finishing steer growth performance, blood biochemical parameters, and carcass characteristics[J].PLoS One, 2014, 9(1):e85406.
[13] OUYANG J L, WANG M Z, HOU Q R, et al.Effects of dietary mulberry leaf powder in concentrate on the rumen fermentation and ruminal epithelium in fattening Hu sheep[J].Animals, 2019, 9(5):218.
[14] ZHANG Y P, YIN C, SCHROYEN M, et al.Effects of the inclusion of fermented mulberry leaves and branches in the gestational diet on the performance and gut microbiota of sows and their offspring[J].Microorganisms, 2021, 9(3):604.
[15] ZHAO X J, LI L, LUO Q L, et al.Effects of mulberry (Morus alba L.) leaf polysaccharides on growth performance, diarrhea, blood parameters, and gut microbiota of early-weanling pigs[J].Livestock Science, 2015, 177:88-94.
[16] CHEN G S, SU Y Y, CAI Y, et al.Comparative transcriptomic analysis reveals beneficial effect of dietary mulberry leaves on the muscle quality of finishing pigs[J].Veterinary Medicine and Science, 2019, 5(4):526-535.  
[17] DING Y N, JIANG X D, YAO X F, et al.Effects of feeding fermented mulberry leaf powder on growth performance, slaughter performance, and meat quality in chicken broilers[J].Animals, 2021, 11(11):3294.
[18] MUN D, KYOUNG H, KONG M, et al.Effects of bacillus-based probiotics on growth performance, nutrient digestibility, and intestinal health of weaned pigs[J].Journal of Animal Science and Technology, 2021, 63(6):1314-1327.  
[19] LAN R X, KIM I H.Effects of Bacillus licheniformis and Bacillus subtilis complex on growth performance and faecal noxious gas emissions in growing-finishing pigs[J].Journal of the Science of Food and Agriculture, 2019, 99(4):1554-1560.  
[20] ZHANG S, YOO D H, AO X, et al.Effects of dietary probiotic, liquid feed and nutritional concentration on the growth performance, nutrient digestibility and fecal score of weaning piglets[J].Asian-Australasian Journal of Animal Sciences, 2020, 33(10):1617-1623.  
[21] KIROS T G, LUISE D, DERAKHSHANI H, et al.Effect of live yeast Saccharomyces cerevisiae supplementation on the performance and cecum microbial profile of suckling piglets[J].PLoS One, 2019, 14(7):e0219557.
[22] RAMOS O Y, BASUALDO M, LIBONATTI C, et al.Current status and application of lactic acid bacteria in animal production systems with a focus on bacteria from honey bee colonies[J].Journal of Applied Microbiology, 2020, 128(5):1248-1260.  
[23] LEE M R, KIM J E, CHOI J Y, et al.Anti-obesity effect in high-fat-diet-induced obese C57BL/6 mice:study of a novel extract from mulberry (Morus alba) leaves fermented with Cordyceps militaris[J].Experimental and Therapeutic Medicine, 2019, 17(3):2185-2193.
[24] HWANG J, SHIEH D E, SHYU Y S, et al.Antidiabetic, antihyperlipidemic, and antioxidant activities of mulberry lees fermented products in diabetic mice[J].Journal of Food Science, 2018, 83(11):2866-2872.  
[25] FAN L J, PENG Y, WU D, et al.Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model[J].Journal of Ethnopharmacology, 2020, 249:112416.
[26] CRONIN P, JOYCE S A, O'TOOLE P W, et al.Dietary fibre modulates the gut microbiota[J].Nutrients, 2021, 13(5):1655.
[27] WANG T T, HE C Q.Pro-inflammatory cytokines:the link between obesity and osteoarthritis[J].Cytokine & Growth Factor Reviews, 2018, 44:38-50.
[28] VAARA S T, GLASSFORD N, EASTWOOD G M, et al.Point-of-care creatinine measurements to predict acute kidney injury[J].Acta Anaesthesiologica Scandinavica, 2020, 64(6):766-773.  
[29] CHEN R H, WANG Q, LI Z F, et al.Studies on effect of Tongfengxiaofang in HUM model mice using a UPLC-ESI-Q-TOF/MS metabolomic approach[J].Biomedical Chromatography, 2021, 35(8):e5118.
[30] LIU Y Y, LI Y H, PENG Y L, et al.Dietary mulberry leaf powder affects growth performance, carcass traits and meat quality in finishing pigs[J].Journal of Animal Physiology and Animal Nutrition, 2019, 103(6):1934-1945.  
[31] MÉNDEZ-SALAZAR E O, MARTÍNEZ-NAVA G A.Uric acid extrarenal excretion:the gut microbiome as an evident yet understated factor in gout development[J].Rheumatology International, 2022, 42(3):403-412.  
[32] HAVLIK J, PLACHY V, FERNANDEZ J, et al.Dietary purines in vegetarian meat analogues[J].Journal of the Science of Food and Agriculture, 2010, 90(14):2352-2357.  
[33] ZHU J X, WANG Y, KONG L D, et al.Effects of biota orientalis extract and its flavonoid constituents, quercetin and rutin on serum uric acid levels in oxonate-induced mice and xanthine dehydrogenase and xanthine oxidase activities in mouse liver[J].Journal of Ethnopharmacology, 2004, 93(1):133-140.  
[34] WANG C P, WANG X, ZHANG X, et al.Morin improves urate excretion and kidney function through regulation of renal organic ion transporters in hyperuricemic mice[J].Journal of Pharmacy and Pharmaceutical Sciences, 2010, 13(3):411-427.  
[35] WANG C P, WANG Y M, WANG X, et al.Mulberroside a possesses potent uricosuric and nephroprotective effects in hyperuricemic mice[J].Planta Medica, 2011, 77(8):786-794.  
[36] GABRIAL S G N, SHAKIB M C R, GABRIAL G N.Protective role of vitamin C intake on muscle damage in male adolescents performing strenuous physical activity[J].Open Access Macedonian Journal of Medical Sciences, 2018, 6(9):1594-1598.  
[37] KOGUCHI T, KOGUCHI H, NAKAJIMA H, et al.Dietary fiber suppresses elevation of uric acid and urea nitrogen concentrations in serum of rats with renal dysfunction induced by dietary adenine[J].International Journal for Vitamin and Nutrition Research, 2004, 74(4):253-263.  
[38] KHOSROSHAHI H T, ABEDI B, GHOJAZADEH M, et al.Effects of fermentable high fiber diet supplementation on gut derived and conventional nitrogenous product in patients on maintenance hemodialysis:a randomized controlled trial[J].Nutrition & Metabolism, 2019, 16:18.
[39] TARRAHI M J, NAMJOO I, BORZOO-ISFAHANI M, et al.Can probiotics supplementation improve glycemic and renal status in diabetic nephropathy?A systematic review and meta-analysis of clinical trials[J].Endocrine, Metabolic & Immune Disorders Drug Targets, 2022, 22(1):143-158.  
[40] ULLAH N, KHAN M A, KHAN S, et al.Nephro-protective potential of Morus alba, a prospective experimental study on animal models[J].Pharmaceutical Biology, 2016, 54(3):530-535.  
[41] SHENG Y, LIU J Y, ZHENG S J, et al.Mulberry leaves ameliorate obesity through enhancing brown adipose tissue activity and modulating gut microbiota[J].Food & Function, 2019, 10(8):4771-4781.  
[42] REBAI O, BELKHIR M, BOUJELBEN A, et al.Morus alba leaf extract mediates neuroprotection against glyphosate-induced toxicity and biochemical alterations in the brain[J].Environmental Science and Pollution Research International, 2017, 24(10):9605-9613.  
[43] KOVACS-NOLAN J, RUPA P, MATSUI T, et al.In vitro and ex vivo uptake of glutathione (GSH) across the intestinal epithelium and fate of oral GSH after in vivo supplementation[J].Journal of Agricultural and Food Chemistry, 2014, 62(39):9499-9506.  
[44] LIN W C, LEE M T, CHANG S C, et al.Effects of mulberry leaves on production performance and the potential modulation of antioxidative status in laying hens[J].Poultry Science, 2017, 96(5):1191-1203.  
[45] LI Z W, CHEN X M, LIU G, et al.Antioxidant activity and mechanism of resveratrol and polydatin isolated from mulberry (Morus alba L.)[J].Molecules, 2021, 26(24):7574.
[46] MENIAILO M E, MALASHCHENKO V V, SHMAROV V A, et al.Interleukin-8 favors pro-inflammatory activity of human monocytes/macrophages[J].International Immunopharmacology, 2018, 56:217-221.
[47] KEIR M, YI T S, LU T, et al.The role of IL-22 in intestinal health and disease[J].The Journal of Experimental Medicine, 2020, 217(3):e20192195.
[48] YANG X Y, PARK G S, LEE M H, et al.Toll-like receptor 4-mediated immunoregulation by the aqueous extract of Mori fructus[J].Phytotherapy Research, 2009, 23(12):1713-1720.  
[49] CHANG B Y, KOO B S, KIM S Y.Pharmacological activities for Morus alba L., focusing on the immunostimulatory property from the fruit aqueous extract[J].Foods, 2021, 10(8):1966.
[50] WANG X, HAO G L, WANG B Y, et al.Function and dysfunction of plasma cells in intestine[J].Cell & Bioscience, 2019, 9:26.
[51] GESSNER D K, BONARIUS M, MOST E, et al.Effects of polyphenol-rich plant products from grape or hop as feed supplements on the expression of inflammatory, antioxidative, cytoprotective and endoplasmic reticulum stress-related genes and the antioxidative status in the liver of piglets[J].Journal of Animal Physiology and Animal Nutrition, 2017, 101(5):e185-e194.
[52] HE X R, FANG J C, RUAN Y L, et al.Structures, bioactivities and future prospective of polysaccharides from Morus alba (white mulberry):a review[J].Food Chemistry, 2018, 245:899-910.
[53] KABURAGI T, YAMANO T, FUKUSHIMA Y, et al.Effect of Lactobacillus johnsonii La1 on immune function and serum albumin in aged and malnourished aged mice[J].Nutrition, 2007, 23(4):342-350.  
[54] LEE J, LEE H J, LEE J J.Coating rice with mulberry leaves rich in deoxynojirimycin ameliorates hyperglycemia and dyslipidemia in C57BL/KsJ db/db mice[J].Nutrition Research and Practice, 2018, 12(6):469-478.  
[55] LEE H J, LEE H, CHOI Y I, et al.Effect of lactic acid bacteria-fermented mulberry leaf extract on the improvement of intestinal function in rats[J].Korean Journal for Food Science of Animal Resources, 2017, 37(4):561-570.  
[56] JIANG Y G, WANG C Y, JIN C, et al.Improved 1-deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism[J].Brazilian Journal of Microbiology, 2014, 45(2):721-729.  
[57] LEE M R, KIM J E, PARK J J, et al.Protective role of fermented mulberry leave extract in LPS induced inflammation and autophagy of RAW264.7 macrophage cells[J].Molecular Medicine Reports, 2020, 22(6):4685-4695.  
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